Abstract: The adsorption of bovine serum albumin (BSA), immunoglobulin G (IgG) and fibrinogen (Fgn) on fluorinated selfassembled monolayers have been studied using time of flight secondary ion mass spectrometry (ToF-SIMS) and Spectroscopic Ellipsometry (SE). The objective of the work has to establish the utility of ToF-SIMS for the determination of the amount of protein adsorbed on the surface. Quantification of surface adsorbed proteins was carried out using SE and a good correlation between ToF-SIMS results and SE was achieved. The surface distribution of proteins were also analysed using Atomic Force Microscopy (AFM). We show that the surface distribution of proteins strongly affect the ToFSIMS results.
Abstract: Coated tool inserts can be considered as the backbone
of machining processes due to their wear and heat resistance.
However, defects of coating can degrade the integrity of these inserts
and the number of these defects should be minimized or eliminated if
possible. Recently, the advancement of coating processes and
analytical tools open a new era for optimizing the coating tools.
First, an overview is given regarding coating technology for cutting
tool inserts. Testing techniques for coating layers properties, as well
as the various coating defects and their assessment are also surveyed.
Second, it is introduced an experimental approach to examine the
possible coating defects and flaws of worn multicoated carbide
inserts using two important techniques namely scanning electron
microscopy and atomic force microscopy. Finally, it is
recommended a simple procedure for investigating manufacturing
defects and flaws of worn inserts.
Abstract: In this study we investigate silica nanoparticle (SiO2- NP) effects on the structure and phase properties of supported lipid monolayers and bilayers, coupling surface pressure measurements, fluorescence microscopy and atomic force microscopy. SiO2-NPs typically in size range of 10nm to 100 nm in diameter are tested. Our results suggest first that lipid molecules organization depends to their nature. Secondly, lipid molecules in the vinicity of big aggregates nanoparticles organize in liquid condensed phase whereas small aggregates are localized in both fluid liquid-expanded (LE) and liquid-condenced (LC). We demonstrated also by atomic force microscopy that by measuring friction forces it is possible to get information as if nanoparticle aggregates are recovered or not by lipid monolayers and bilayers.