Abstract: In vitro plant regeneration has been successfully obtained from basal shoot explant of Vetiveria zizanioides through indirect organogenesis. The explant was cultured in Murashige & Skoog’s (MS) media supplemented with 2,4-D, IAA, and kinetin in various concentrations. Callus was well induced in media supplemented with 2 ppm 2,4-D, 1 ppm IAA, and 1 ppm kinetin. This callus was then transferred to MS media supplemented with 1 - 5 ppm of BAP for shoot regeneration. The media supplemented with 3 ppm BAP was a suitable medium for shoot induction, as well as for shoot multiplication. Rooting was well developed in shoot following transferred to half MS media containing 0.2 ppm IBA. Plantlet was then transferred to husk charcoal for acclimatization, and almost all (90%) of plantlets were survived during acclimatization.
Abstract: Mechanical design of the thin-film solar framed
module and mounting system is important to enhance module
reliability and to increase areas of applications. The stress induced by
different mounting positions played a main role controlling the
stability of the whole mechanical structure. From the finite element
method, under the pressure from the back of module, the stress at Lc
(center point of the Long frame) increased and the stresses at Center,
Corner and Sc (center point of the Short frame) decreased while the
mounting position was away from the center of the module. In addition,
not only the stress of the glass but also the stress of the frame
decreased. Accordingly it was safer to mount in the position away
from the center of the module. The emphasis of designing frame
system of the module was on the upper support of the Short frame.
Strength of the overall structure and design of the corner were also
important due to the complexity of the stress in the Long frame.
Abstract: A numerical study on the influence of electroosmotic flow on analyte preconcentration by isotachophoresis ( ITP) is made. We consider that the double layer induced electroosmotic flow ( EOF) counterbalance the electrophoretic velocity and a stationary ITP stacked zones results. We solve the Navier-Stokes equations coupled with the Nernst-Planck equations to determine the local convective velocity and the preconcentration dynamics of ions. Our numerical algorithm is based on a finite volume method along with a secondorder upwind scheme. The present numerical algorithm can capture the the sharp boundaries of step-changes ( plateau mode) or zones of steep gradients ( peak mode) accurately. The convection of ions due to EOF reduces the resolution of the ITP transition zones and produces a dispersion in analyte zones. The role of the electrokinetic parameters which induces dispersion is analyzed. A one-dimensional model for the area-averaged concentrations based on the Taylor-Aristype effective diffusivity is found to be in good agreement with the computed solutions.
Abstract: Based on experimental data using accelerometry technology there was developed an analytical model that approximates human induced ground reaction forces in vertical, longitudinal and lateral directions ascending and descending the stairs. Proposed dynamic loading factors and corresponding phase shifts for the first five harmonics of continuous walking force history in case of stair ascend and descend. Into account is taken imperfectness of individual footfall forcing functions, differences between continuous walking force histories among individuals. There is proposed mean synthetic continuous walking force history that can be used in numerical simulations of human movement on the stairs.
Abstract: In this paper, the effect of atmospheric turbulence on
bit error probability in free-space optical CDMA scheme with
Sequence Inverse Keyed (SIK) optical correlator receiver is analyzed.
Here Intensity Modulation scheme is considered for transmission.
The turbulence induced fading is described by the newly introduced
gamma-gamma pdf[1] as a tractable mathematical model for
atmospheric turbulence. Results are evaluated with Gold and Kasami
code & it is shown that Gold sequence can be used for more
efficient transmission than Kasami sequence in an atmospheric
turbulence channel.
Abstract: Experimental investigations were carried out in the
Manchester Tidal flow Facility (MTF) to study the flow patterns in
the region around and adjacent to a hypothetical headland in tidal
(oscillatory) ambient flow. The Planar laser-induced fluorescence
(PLIF) technique was used for visualization, with fluorescent dye
released at specific points around the headland perimeter and in its
adjacent recirculation zone. The flow patterns can be generalized into
the acceleration, stable flow and deceleration stages for each halfcycle,
with small variations according to location, which are more
distinct for low Keulegan-Carpenter number (KC) cases. Flow
patterns in the mixing region are unstable and complex, especially in
the recirculation zone. The flow patterns are in agreement with
previous visualizations, and support previous results in steady
ambient flow. It is suggested that the headland lee could be a viable
location for siting of pollutant outfalls.
Abstract: Heat-inducible gene expression vectors are useful for hyperthermia-induced cancer gene therapy, because the combination
of hyperthermia and gene therapy can considerably improve the therapeutic effects. In the present study, we developed an enhanced
heat-inducible transgene expression system in which a heat-shock
protein (HSP) promoter and tetracycline-responsive transactivator
were combined. When the transactivator plasmid containing the
tetracycline-responsive transactivator gene was co-transfected with
the reporter gene expression plasmid, a high level of heat-induced gene expression was observed compared with that using the HSP
promoter without the transactivator. In vitro evaluation of the
therapeutic effect using HeLa cells showed that heat-induced therapeutic gene expression caused cell death in a high percentage of
these cells, indicating that this strategy is promising for cancer gene therapy.
Abstract: Recent theorizations on the cognitive process of moral
judgment have focused on the role of intuitions and emotions, marking
a departure from previous emphasis on conscious, step-by-step
reasoning. My study investigated how being in a disgusted mood state
affects moral judgment.
Participants were induced to enter a disgusted mood state through
listening to disgusting sounds and reading disgusting descriptions.
Results shows that they, when compared to control who have not been
induced to feel disgust, are more likely to endorse actions that are
emotionally aversive but maximizes utilitarian return
The result is analyzed using the 'emotion-as-information' approach
to decision making. The result is consistent with the view that
emotions play an important role in determining moral judgment.
Abstract: Numerical studies have been carried out using a
validated two-dimensional RNG k-epsilon turbulence model for the
design optimization of a thrust vector control system using shock
induced supersonic secondary jet. Parametric analytical studies have
been carried out with various secondary jets at different divergent
locations, jet interaction angles, jet pressures. The results from the
parametric studies of the case on hand reveal that the primary nozzle
with a small divergence angle, downstream injections with a distance
of 2.5 times the primary nozzle throat diameter from the primary
nozzle throat location warrant higher efficiency over a certain range
of jet pressures and jet angles. We observed that the supersonic
secondary jet opposing the core flow with jets interaction angle of
40o to the axis far downstream of the nozzle throat facilitates better
thrust vectoring than the secondary jet with same direction as that of
core flow with various interaction angles. We concluded that fixing
of the supersonic secondary jet nozzle pointing towards the throat
direction with suitable angle at a distance 2 to 4 times of the primary
nozzle throat diameter, as the case may be, from the primary nozzle
throat location could facilitate better thrust vectoring for the
supersonic aerospace vehicles.
Abstract: In the territories where high-intensity
earthquakes are frequent is paid attention to the solving of the
seismic problems. In the paper are described two
computational model variants based on finite element method
of the construction with different subsoil simulation (rigid or
elastic subsoil) is used. For simulation and calculations
program system based on method final elements ANSYS was
used. Seismic responses calculations of residential building
structure were effected on loading characterized by
accelerogram for comparing with the responses spectra
method.
Abstract: The process of laser absorption in the skin during
laser irradiation was a critical point in medical application
treatments. Delivery the correct amount of laser light is a critical
element in photodynamic therapy (PDT). More amounts of laser
light able to affect tissues in the skin and small amount not able to
enhance PDT procedure in skin. The knowledge of the skin tone
laser dependent distribution of 635 nm radiation and its penetration
depth in skin is a very important precondition for the investigation of
advantage laser induced effect in (PDT) in epidermis diseases
(psoriasis). The aim of this work was to estimate an optimum effect
of diode laser (635 nm) on the treatment of epidermis diseases in
different color skin. Furthermore, it is to improve safety of laser in
PDT in epidermis diseases treatment. Advanced system analytical
program (ASAP) which is a new approach in investigating the PDT,
dependent on optical properties of different skin color was used in
present work. A two layered Realistic Skin Model (RSM); stratum
corneum and epidermal with red laser (635 nm, 10 mW) were used
for irradiative transfer to study fluence and absorbance in different
penetration for various human skin colors. Several skin tones very
fair, fair, light, medium and dark are used to irradiative transfer. This
investigation involved the principles of laser tissue interaction when
the skin optically injected by a red laser diode. The results
demonstrated that the power characteristic of a laser diode (635 nm)
can affect the treatment of epidermal disease in various color skins.
Power absorption of the various human skins were recorded and
analyzed in order to find the influence of the melanin in PDT
treatment in epidermal disease. A two layered RSM show that the
change in penetration depth in epidermal layer of the color skin has a
larger effect on the distribution of absorbed laser in the skin; this is
due to the variation of the melanin concentration for each color.
Abstract: The objective of this work is to present a expertise on
flooding hazard analysis and how to reduce the risk. The analysis
concerns the disaster induced by the flood on November 10/11, 2001
in the Bab El Oued district of the city of Algiers.The study begins by
an expertise of damages in related with the urban environment and
the history of the urban growth of the site. After this phase, the work
is focalized on the identification of the existing correlations between
the development of the town and its vulnerability. The final step
consists to elaborate the interpretations on the interactions between
the urban growth, the sewerage network and the vulnerability of the
urban system.In conclusion, several recommendations are formulated
permitting the mitigation of the risk in the future. The principal
recommendations concern the new urban operations and the existing
urbanized sites.
Abstract: The solvated electron is self-trapped (polaron) owing
to strong interaction with the quantum polarization field. If the
electron and quantum field are strongly coupled then the collective
localized state of the field and quasi-particle is formed. In such a
formation the electron motion is rather intricate. On the one hand the
electron oscillated within a rather deep polarization potential well
and undergoes the optical transitions, and on the other, it moves
together with the center of inertia of the system and participates in
the thermal random walk. The problem is to separate these motions
correctly, rigorously taking into account the conservation laws. This
can be conveniently done using Bogolyubov-Tyablikov method of
canonical transformation to the collective coordinates. This
transformation removes the translational degeneracy and allows one
to develop the successive approximation algorithm for the energy and
wave function while simultaneously fulfilling the law of conservation
of total momentum of the system. The resulting equations determine
the electron transitions and depend explicitly on the translational
velocity of the quasi-particle as whole. The frequency of optical
transition is calculated for the solvated electron in ammonia, and an
estimate is made for the thermal-induced spectral bandwidth.
Abstract: In a previously developed fast vortex method, the
diffusion of the vortex sheet induced at the solid wall by the no-slip
boundary conditions was modeled according to the approximation
solution of Koumoutsakos and converted into discrete blobs in the
vicinity of the wall. This scheme had been successfully applied to a
simulation of the flow induced with an impulsively initiated circular
cylinder. In this work, further modifications on this vortex method are
attempted, including replacing the approximation solution by the
boundary-element-method solution, incorporating a new algorithm for
handling the over-weak vortex blobs, and diffusing the vortex sheet
circulation in a new way suitable for high-curvature solid bodies. The
accuracy is thus largely improved. The predictions of lift and drag
coefficients for a uniform flow past a NASA airfoil agree well with the
existing literature.
Abstract: This paper presents the study of induced currents and
temperature distribution in gear heated by induction process using 2D
finite element (FE) model. The model is developed by coupling
Maxwell and heat transfer equations into a multi-physics model. The
obtained results allow comparing the medium frequency (MF) and
high frequency (HF) cases and the effect of machine parameters on
the evolution of induced currents and temperature during heating.
The sensitivity study of the temperature profile is conducted and the
case hardness is predicted using the final temperature profile. These
results are validated using tests and give a good understanding of
phenomena during heating process.
Abstract: A scaffold is necessary for tooth regeneration because of its three-dimensional geometry. For restoration of defect, it is necessary for the scaffold to be prepared in the shape of the defect. Sponges made from polyvinyl alcohol with formalin cross-linking (PVF sponge) have been used for scaffolds for bone formation in vivo. To induce osteogenesis within the sponge, methods of growing rat bone marrow cells (rBMCs) among the fiber structures in the sponge might be considered. Storage of rBMCs among the fibers in the sponge coated with dextran (10 kDa) was tried. After seeding of rBMCs to PVF sponge immersed in dextran solution at 2 g/dl concentration, osteogenesis was recognized in subcutaneously implanted PVF sponge as a scaffold in vivo. The level of osteocalcin was 25.28±5.71 ng/scaffold and that of Ca was 129.20±19.69 µg/scaffold. These values were significantly higher than those in sponges without dextran coating (p
Abstract: F-actin fibrils are the cytoskeleton of osteocytes. They react in a dynamic manner to mechanical loading, and strength and
reposition their efforts to reinforce the cells structure. We hypothesize that f-actin is temporarly disrupted after loading and repolymerizes
in a new orientation to oppose the applied load. In vitro studies are conducted to determine f-actin disruption after varying mechanical stimulus parameters that are known to affect bone
formation. Results indicate that the f-actin cytoskeleton is disrupted in vitro as a function of applied mechanical stimulus parameters and
that the f-actin bundles reassemble after loading induced disruption
within 3 minutes after cessation of loading. The disruption of the factin
cytoskeleton depends on the magnitude of stretch, the numbers
of loading cycles, frequency, the insertion of rest between loading
cycles and extracellular calcium. In vivo studies also demonstrate
disruption of the f-actin cytoskeleton in cells embedded in the bone
matrix immediately after mechanical loading. These studies suggest
that adaptation of the f-actin fiber bundles of the cytoskeleton in
response to applied loads occurs by disruption and subsequent repolymerization.
Abstract: Engineered nanoparticles’ usage rapidly increased in
various applications in the last decade due to their unusual properties.
However, there is an ever increasing concern to understand their
toxicological effect in human health. Particularly, metal and metal
oxide nanoparticles have been used in various sectors including
biomedical, food and agriculture. But their impact on human health is
yet to be fully understood. In this present investigation, we assessed
the toxic effect of engineered nanoparticles (ENPs) including Ag,
MgO and Co3O4 nanoparticles (NPs) on human mesenchymal stem
cells (hMSC) adopting cell viability and cellular morphological
changes as tools The results suggested that silver NPs are more toxic
than MgO and Co3O4NPs. The ENPs induced cytotoxicity and
nuclear morphological changes in hMSC depending on dose. The cell
viability decreases with increase in concentration of ENPs. The
cellular morphology studies revealed that ENPs damaged the cells.
These preliminary findings have implications for the use of these
nanoparticles in food industry with systematic regulations.
Abstract: Electron multiplying charge coupled devices (EMCCDs) have revolutionized the world of low light imaging by introducing on-chip multiplication gain based on the impact ionization effect in the silicon. They combine the sub-electron readout noise with high frame rates. Signal-to-noise Ratio (SNR) is an important performance parameter for low-light-level imaging systems. This work investigates the SNR performance of an EMCCD operated in Non-inverted Mode (NIMO) and Inverted Mode (IMO). The theory of noise characteristics and operation modes is presented. The results show that the SNR of is determined by dark current and clock induced charge at high gain level. The optimum SNR performance is provided by an EMCCD operated in NIMO in short exposure and strong cooling applications. In contrast, an IMO EMCCD is preferable.
Abstract: In areas of low to moderate seismicity many building contents and equipment are not positively fixed to the floor or tied to adjacent walls. Under seismic induced horizontal vibration, such contents and equipment can suffer from damage by either overturning or impact associated with rocking. This paper focuses on the estimation of shock on typical contents and equipment due to rocking. A simplified analytical model is outlined that can be used to estimate the maximum acceleration on a rocking object given its basic geometric and mechanical properties. The developed model was validated against experimental results. The experimental results revealed that the maximum shock acceleration can be underestimated if the static stiffness of the materials at the interface between the rocking object and floor is used rather than the dynamic stiffness. Excellent agreement between the model and experimental results was found when the dynamic stiffness for the interface material was used, which was found to be generally much higher than corresponding static stiffness under different investigated boundary conditions of the cushion. The proposed model can be a beneficial tool in performing a rapid assessment of shock sensitive components considered for possible seismic rectification.