In vitro Plant Regeneration of Java Vetiver (Vetiveria zizanioides)
In vitro plant regeneration has been successfully obtained from basal shoot explant of Vetiveria zizanioides through indirect organogenesis. The explant was cultured in Murashige & Skoog’s (MS) media supplemented with 2,4-D, IAA, and kinetin in various concentrations. Callus was well induced in media supplemented with 2 ppm 2,4-D, 1 ppm IAA, and 1 ppm kinetin. This callus was then transferred to MS media supplemented with 1 - 5 ppm of BAP for shoot regeneration. The media supplemented with 3 ppm BAP was a suitable medium for shoot induction, as well as for shoot multiplication. Rooting was well developed in shoot following transferred to half MS media containing 0.2 ppm IBA. Plantlet was then transferred to husk charcoal for acclimatization, and almost all (90%) of plantlets were survived during acclimatization.
[1] Leupin, R. E., M. Leupin, C. Ehret, K. H. Erismann, and B. Witholt.
2000. Compact callus induction and plant reneration of a non-flowering
vetiver from Java. Plant Cell, Tissue and Organ Culture. 62: 115-123.
[2] Danha, L. T., Mamucari, P. Truog, and N. Foester. 2009. Response
surface method applied to super critical carbon dioxide extraction of
Vetiveria zizanioides essential oil. Engineering Journal. 155 : 617-626.
[3] Greenfield, J. C. 2002. Vetiver Grass An Essential Grass for The
Conservation of Planet Earth. Infinity Publishing. Pennsylvania
[4] Srivastava, J., H. Chandra, S. Nandita. 2007. Allelophatic Response of
Vetiveria zizanioides on Members of the Family Enterobacteriaceae
and Pseudomonas spp. Environmentalist. 27(10): 253-260.
[5] Indrawanto, C., Eriyatno, A. M. Fauzi, Machfud, Sukardi, dan N.
Soetrisno. 2008. Forecasting of Vetiver Prices: An Application of
artificial Neural Network Method. Indonesian Journal of Agriculture.
1(1): 58-63.
[6] George, E. F., and P. D. Sherrington. 1984. Plant Propagation by Tissue
Culture. Exegetics Ltd. P 709.
[7] Neumann, K. H., A. Kumar and J. Imani. 2009. Tissue Culture – a Tool
in Biotechnology. Springer-Verlag. Berlin. Pp. 79
[8] Schulze, J. 2007. Improvements in Cereal Tissue Culture by
Thidiazuron: A Review. Fruit, Vegetable and Cereal Science and
Biotechnology. 1: 64 – 79
[9] Yu, H., W. Wang, Y. Wang and B. Hou. 2012. High frequency wheat
regeneration from leaf tissue explants of regenerated plantlets. Adv.
Biosci. & Biotech. 3 : 46 - 50
[10] Mucciarelli, M., M. Gallino, S. Scannerini, and M. Maffei. 1993. Callus
induction and plant regeneration in Vetiveria zizanioides. Plant Cell,
Tissue and Organ Culture. 35: 267- 271.
[11] Sangduen, N. and S. Prasertsongskun. 2009. Regeneration and
application: from suspension cultured-derived inflorescence of Vetiveria
zizanioides (L.) Nash. to selection of herbicide resistant cells. Au. J. T.
12 : 135 – 148
[12] Murashige, T. and F. Skoog. 1962. A Revised Medium for Rapid
Growth and Bio Assays with Tobacco Tissue Cultures. Physiol. Plant.
15 : 473 - 497
[13] Klerk, J., Hall, M. A., George, E. F. dan Geert, M. (2008): Plant
Propagation by Tissue Culture 3rdEdition, Springer, Netherlands, 175-
205.
[14] Rao, A. M., Kavi, K. P. B. dan Ananda, R. L. (1998): Callus Induction
and High Frequecy Plant Regeneration in Italia Millet (Setaria italica).
Plant Cell Report, 7, 557-559.
[15] Ohnoutkova, L., Ehrenbergerova, J. dan Serhantova, V. (2004) : Callus
Induction and Regeneration Efficiency of Spring Barley Cultivar
Registered in the Czech Republic, Journal of Plant Soil Environmental,
50 (10), 456-462.
[16] Rashid, U., S. Ali., G. M. Ali., N. Ayub., and M. S. Masood. 2009.
Establishment of an efficient callus induction and plant regeneration
system in Pakistani wheat (Triticum aestivum) cultivars. Electronic
Journal of Biotechnology. 12 (3): 1-12.
[17] Davis, M. J., R. Baker, and J. J. Hanan. 1977. Clonal multiplication of
carnation by micropropagation. J. Am. Soc. Hortic. Sci. 102: 48-53.
[18] Kobayashi, K. B. Zbell, and J. Reinert. 1981. A high affinity binding site
for cytokinin to a particulate fraction in carrot suspension cells.
Protoplasma. 106 : 145 – 155.
[19] Bhojwani, S. S. dan Razdan, M. K. (1983): Plant Tissue Culture: Theory
and Practice, Elsevier, Amsterdam, 25-42.
[20] Hartmann, H. T, D. E. Kester, F. T. Davies, R. L. Gene. 1997. Plant
propagation. principles and practices (sixth ed). Prentice Hall. p. 75
[21] Santoso and Nursandi. 2002. Kultur Jaringan Tanaman. Penerbit Univ.
Muhammadiyah Malang. Malan.
[1] Leupin, R. E., M. Leupin, C. Ehret, K. H. Erismann, and B. Witholt.
2000. Compact callus induction and plant reneration of a non-flowering
vetiver from Java. Plant Cell, Tissue and Organ Culture. 62: 115-123.
[2] Danha, L. T., Mamucari, P. Truog, and N. Foester. 2009. Response
surface method applied to super critical carbon dioxide extraction of
Vetiveria zizanioides essential oil. Engineering Journal. 155 : 617-626.
[3] Greenfield, J. C. 2002. Vetiver Grass An Essential Grass for The
Conservation of Planet Earth. Infinity Publishing. Pennsylvania
[4] Srivastava, J., H. Chandra, S. Nandita. 2007. Allelophatic Response of
Vetiveria zizanioides on Members of the Family Enterobacteriaceae
and Pseudomonas spp. Environmentalist. 27(10): 253-260.
[5] Indrawanto, C., Eriyatno, A. M. Fauzi, Machfud, Sukardi, dan N.
Soetrisno. 2008. Forecasting of Vetiver Prices: An Application of
artificial Neural Network Method. Indonesian Journal of Agriculture.
1(1): 58-63.
[6] George, E. F., and P. D. Sherrington. 1984. Plant Propagation by Tissue
Culture. Exegetics Ltd. P 709.
[7] Neumann, K. H., A. Kumar and J. Imani. 2009. Tissue Culture – a Tool
in Biotechnology. Springer-Verlag. Berlin. Pp. 79
[8] Schulze, J. 2007. Improvements in Cereal Tissue Culture by
Thidiazuron: A Review. Fruit, Vegetable and Cereal Science and
Biotechnology. 1: 64 – 79
[9] Yu, H., W. Wang, Y. Wang and B. Hou. 2012. High frequency wheat
regeneration from leaf tissue explants of regenerated plantlets. Adv.
Biosci. & Biotech. 3 : 46 - 50
[10] Mucciarelli, M., M. Gallino, S. Scannerini, and M. Maffei. 1993. Callus
induction and plant regeneration in Vetiveria zizanioides. Plant Cell,
Tissue and Organ Culture. 35: 267- 271.
[11] Sangduen, N. and S. Prasertsongskun. 2009. Regeneration and
application: from suspension cultured-derived inflorescence of Vetiveria
zizanioides (L.) Nash. to selection of herbicide resistant cells. Au. J. T.
12 : 135 – 148
[12] Murashige, T. and F. Skoog. 1962. A Revised Medium for Rapid
Growth and Bio Assays with Tobacco Tissue Cultures. Physiol. Plant.
15 : 473 - 497
[13] Klerk, J., Hall, M. A., George, E. F. dan Geert, M. (2008): Plant
Propagation by Tissue Culture 3rdEdition, Springer, Netherlands, 175-
205.
[14] Rao, A. M., Kavi, K. P. B. dan Ananda, R. L. (1998): Callus Induction
and High Frequecy Plant Regeneration in Italia Millet (Setaria italica).
Plant Cell Report, 7, 557-559.
[15] Ohnoutkova, L., Ehrenbergerova, J. dan Serhantova, V. (2004) : Callus
Induction and Regeneration Efficiency of Spring Barley Cultivar
Registered in the Czech Republic, Journal of Plant Soil Environmental,
50 (10), 456-462.
[16] Rashid, U., S. Ali., G. M. Ali., N. Ayub., and M. S. Masood. 2009.
Establishment of an efficient callus induction and plant regeneration
system in Pakistani wheat (Triticum aestivum) cultivars. Electronic
Journal of Biotechnology. 12 (3): 1-12.
[17] Davis, M. J., R. Baker, and J. J. Hanan. 1977. Clonal multiplication of
carnation by micropropagation. J. Am. Soc. Hortic. Sci. 102: 48-53.
[18] Kobayashi, K. B. Zbell, and J. Reinert. 1981. A high affinity binding site
for cytokinin to a particulate fraction in carrot suspension cells.
Protoplasma. 106 : 145 – 155.
[19] Bhojwani, S. S. dan Razdan, M. K. (1983): Plant Tissue Culture: Theory
and Practice, Elsevier, Amsterdam, 25-42.
[20] Hartmann, H. T, D. E. Kester, F. T. Davies, R. L. Gene. 1997. Plant
propagation. principles and practices (sixth ed). Prentice Hall. p. 75
[21] Santoso and Nursandi. 2002. Kultur Jaringan Tanaman. Penerbit Univ.
Muhammadiyah Malang. Malan.
@article{"International Journal of Biological, Life and Agricultural Sciences:52003", author = "Iriawati and R. R. Esyanti and W. Natalia and N. Zahya", title = "In vitro Plant Regeneration of Java Vetiver (Vetiveria zizanioides)", abstract = "In vitro plant regeneration has been successfully obtained from basal shoot explant of Vetiveria zizanioides through indirect organogenesis. The explant was cultured in Murashige & Skoog’s (MS) media supplemented with 2,4-D, IAA, and kinetin in various concentrations. Callus was well induced in media supplemented with 2 ppm 2,4-D, 1 ppm IAA, and 1 ppm kinetin. This callus was then transferred to MS media supplemented with 1 - 5 ppm of BAP for shoot regeneration. The media supplemented with 3 ppm BAP was a suitable medium for shoot induction, as well as for shoot multiplication. Rooting was well developed in shoot following transferred to half MS media containing 0.2 ppm IBA. Plantlet was then transferred to husk charcoal for acclimatization, and almost all (90%) of plantlets were survived during acclimatization.
", keywords = "Callus, plantlet regeneration, shoot induction, Vetiveria zizanioides.", volume = "7", number = "9", pages = "833-3", }
