Abstract: The main objective of this study was to determine the effects
of vermicompost and amino acids on the qualitative and quantitative yield of
chamomile. The experiment was conducted during the growing season of
2010 at the Alborz Medical Research Center. The Treatment groups consisted
of vermicompost (0, 5, 10, 15 and 20 tons/ha) and the sprays of amino acids
(budding stag, flowering stage, and budding + flowering stage). The
experimental design was a factorial experiment based on Randomized
Complete Block Design (RCBD) with three replications. The present results
have shown that the highest plant height, flower head diameter, fresh and dry
flower yield and significant essential oil content were obtained by using 20-
ton vermicompost per hectare. Effects of amino acids were similar to those
seen in vermicompost treatment and all measured traits were seen to be
significant after the spray of amino acids at the budding + flowering stage).
Abstract: Plant tissue culture is an important in vitro technology applied for agricultural and industrial production. A sterile condition of culture medium is one of the main aspects. The alternative technique for medium sterilization to replace autoclaving was carried out. For sterilization of plant tissue culture medium without autoclaving, ten commercial pure essential oils and 5 disinfectants were tested. Each essential oil or disinfectant was added to a 20-mL Murashige and Skoog (MS) medium before medium was solidified in a 120-mL container, kept for 2 weeks before evaluating sterile conditions. Treated media, supplemented with essential oils or disinfectants, were compared to control medium, autoclaved at 121 degree Celsius for 15 min. Sterile conditions of MS medium were found 100% from betel oil or clove oil (18 mL/20 mL medium), cinnamon oil (36 mL/20 mL medium), lavender oil or holy basil oil (108 mL/20 mL medium), and lemon oil or tea tree oil or turmeric oil (252 mL/20 mL medium), compared to 100% sterile condition from autoclaved medium. For disinfectants, 2% iodine + 2.4% potassium iodide, 2% merbromine solution, 10% povidone-iodine, 6% sodium hypochlorite or 0.1% thimerosal at 36 mL/20 mL medium provided 100% sterile conditions. Furthermore, growth of new shoots from chrysanthemum node explants on treated media (fresh weight, shoot length, root length and number of node) were also reported and discussed in the comparison of those on autoclaved medium.
Abstract: This research is to study the performance of heat
pump dryer for drying of kaffir lime leaves under different media and
to compare the color values and essential oil content of final products
after drying. In the experiments, kaffir lime leaves were dried in the
closed-loop system at drying temperatures of 40, 50 and 60 oC. The
drying media used in this study were hot air, CO2 and N2 gases. The
velocity of drying media in the drying chamber was 0.4 m/s with
bypass ratio of 30%. The initial moisture content of kaffir lime leaves
was approximately 180-190 % d.b. It was dried until down to a final
moisture content of 10% d.b. From the experiments, the results
showed that drying rate, the coefficient of performance (COP) and
specific energy consumption (SEC) depended on drying temperature.
While drying media did not affect on drying rate. The time for kaffir
lime leaves drying at 40, 50 and 60 oC was 10, 5 and 3 hours,
respectively. The performance of the heat pump system decreased
with drying temperature in the range of 2.20-3.51. In the aspect of
final product color, the greenness and overall color had a great
change under drying temperature at 60 oC rather than drying at 40
and 50 oC. When compared among drying media, the greenness and
overall color of product dried with hot air at 60 oC had a great change
rather than dried with CO2 and N2.
Abstract: The plant world is the source of many medicines.
Recently, researchers have estimated that there are approximately
400,000 plant species worldwide, of which about a quarter or a third
have been used by societies for medicinal purposes. The human uses
of plants for thousands of years to treat various ailments, in many
developing countries, much of the population trust in traditional
doctors and their collections of medicinal plants to treat them.
Essential oils have many therapeutic properties. In herbal medicine,
they are used for their antiseptic properties against infectious
diseases of fungal origin, against dermatophytes, those of bacterial
origin. The aim of our study is to determine the antimicrobial effect
of essential oils of the plant Trigonella focnum greacum on some
pathogenic bacteria, it is a medicinal plant used in traditional
therapy. The test adopted is based on the diffusion method on solid
medium (Antibiogram), this method determines the sensitivity or
resistance of a microorganism vis-à-vis the extract studied. Our study
reveals that the essential oil of the plant Trigonella focnum greacum
has a different effect on the resistance of germs. For staphiloccocus
Pseudomonnas aeroginosa and Krebsilla, are moderately sensitive
strains, also Escherichia coli and Candida albicans represents a high
sensitivity. By against Proteus is a strain that represents a weak
sensitivity.
Abstract: Our results showed that for the growth of qualitative
seedling and vegetative raw material of ðó. marschallianus Willd. and
T. serphyllum L. it is more profitable to use the in vitro and
hydroponics combined method. In in vitro culture it is possible to do
micro-propagation whole year with 98-99% rhizogenesis. 30000
micro-plants were obtained from one explant during 9 months.
Hydroponic conditions provide the necessary microclimate for
microplants where the survival rate without acclimatization was
93.3%. The essential oil content in hydroponic dry herb of both
species in vegetative and blossom phase was 1.3% whereas in wild
plants it was 1.2%, the content of extractive substances and vitamin
C also exceeded wild plants. Our biochemical and radiochemical
investigations indicated that the medicinal raw materials obtained
from hydroponic and wild plants of Thymus species correspond to
the demands of SPh XI, and the content of artificial radionuclides
does not exceed the MACL.
Abstract: The purpose of this study was to elucidate the factors affecting antimicrobial effectiveness of essential oils against food spoilage and pathogenic bacteria. The minimum inhibition concentrations (MIC) of the essential oils, were determined by turbidimetric technique using Biocreen C, analyzer. The effects of pH ranging from 7.3 to 5.5 in absence and presence of essential oils and/or NaCl on the lag time and mean generation time of the bacteria at 370C, were carried out and results were determined showed that, combination of low pH and essential oil at 370C had additive effects against the test micro-organisms. The combination of 1.2 % (w/v) of NaCl and clove essential oil at 0.0325% (v/v) was effective against E. coli. The use of concentrations less than MIC in combination with low pH and or NaCl has the potential of being used as an alternative to “traditional food preservatives".
Abstract: This study was conducted to evaluate the antifungal
activities of Cinnamomum zeylanicum and Origanum vulgare L.
essential oil against Aspergillus flavus in culture media and tomato
paste. 200 ppm of cinnamon and 500 ppm of oregano completely
inhibited A. flavus growth in culture media, while in tomato paste 300
ppm of cinnamon and 200 ppm of oregano had the same effect. Test
panel evaluations revealed that samples with 100 and 200 ppm
cinnamon were acceptable. The results may suggest the potential use
of Cinnamomum zeylanicum essential oil as natural preservative in
tomato paste.
Abstract: Coriander is an annual and herbaceous plant, belong to
the apiaceae family. This plant is cultivated world widely. It is well
known for having medicinal properties. The aim of this experiment
was to study seed quality of species grown in Kermanshah
conditions. The experiment was carried out in research farm, Campus
of Agriculture and Natural Resources, Razi University, Kermanshah,
Iran. Coriander (local type) was grown in late spring May (5th and
20th) and Jun (4th and 19th), and plant density (10, 30, 50 and 70
plants m-2) in 2009. The experimental plots were laid out in a
factorial according to a randomized complete block design with three
replications. The fruits were harvest between 83.5 – 106.5 days after
sowing. The essential oil and oil content was extracted by Clevenger
and Soxhlet apparatuses, respectively. Results showed that delay at
planting date increased the oil content. Also, with the increase at
plant density was decreased oil content and essential oil.
Abstract: The traditional method for essential oil extraction from agarwood (Aquilaria Crassna) is to soak it in water and follow with hydrodistillation. The effect of various agarwood pretreatments: ethanol, acid, alkaline, enzymes, and ultrasound, and the effect of subcritical water extraction(SWE) was studied to compare with the traditional method. The major compositions of agarwood oil from hydrodistillation were aroma compounds as follow: aristol-9-en-8- one (21.53%), selina-3, 7(11)-diene (12.96%), τ-himachalene (9.28%), β-guaiene (5.79%), hexadecanoic acid (4.90%) and guaia- 3,9-diene (4.21%). Whereas agarwood oil from pretreatments with ethanol and ultrasound, and SWE got fatty acid compounds. Extraction of agarwood oil using these pretreatments could improve the agarwood oil yields up to 2 times that of the traditional method. The components of the pretreated sample with diluted acid (H2SO4) at pH 4 gave quite similar results as the traditional method. Therefore, the enhancement of essential oil from agarwood depends on requirement of type of extracted oil that involved extraction methods.
Abstract: Proteins levels produced by bacteria may be increased
in stressful surroundings, such as in the presence of antibiotics. It
appears that many antimicrobial agents or antibiotics, when used at
low concentrations, have in common the ability to activate or repress
gene transcription, which is distinct from their inhibitory effect.
There have been comparatively few studies on the potential of
antibiotics or natural compounds in nature as a specific chemical
signal that can trigger a variety of biological functions. Therefore,
this study was focusing on the effect of essential oils from
Cymbopogon flexuosus and C. nardus in regulating proteins
production by Bacillus subtilis ATCC 21332. The Minimum
Inhibition Concentrations (MICs) of both essential oils on B. subtilis
were determined by using microdilution assay, resulting 0.2% and
1.56% for each C. flexuosus and C. nardus subsequently. The
bacteria were further exposed to each essential oils at concentration
of 0.01XMIC for 2 days. The proteins were then isolated and
analyzed by sodium dodecyl sulfate polyacrylamide gel
electrophoresis (SDS-PAGE). Protein profile showed that a band
with approximate size of 250 kD was appeared for the treated
bacteria with essential oils. Thus, Bacillus subtilis ATCC 21332 in
stressful condition with the presence of essential oils at low
concentration could induce the protein production.
Abstract: Team distillation assisted by microwave extraction
(SDAM) considered as accelerated technique extraction is a
combination of microwave heating and steam distillation, performed
at atmospheric pressure. SDAM has been compared with the same
technique coupled with the cryogrinding of seeds (SDAM -CG).
Isolation and concentration of volatile compounds are performed by a
single stage for the extraction of essential oil from Cuminum
cyminum seeds. The essential oils extracted by these two methods for
5 min were quantitatively (yield) and qualitatively (aromatic profile)
no similar. These methods yield an essential oil with higher amounts
of more valuable oxygenated compounds, and allow substantial
savings of costs, in terms of time, energy and plant material. SDAM
and SDAM-CG is a green technology and appears as a good
alternative for the extraction of essential oils from aromatic plants.
Abstract: The essential oil of M. × piperita L. was formulated
into a topical gel. The prepared gel was characterized for its pH,
viscosity, spreadiblity, consistency and extrudiblity, while its stability
was evaluated under different temperature conditions. The prepared
M. × piperita oil gel was clear and transparent. The pH value of
developed gel was 6.6, while its viscosity was 1200 cP. Spreadability
and consistency of the M. × piperita oil gel was 10.7 g.cm/sec and 7
mm, respectively. The prepared gel showed good extrudiblity. During
the stability studies, no significant change in pH and viscosity as a
function of time for gel was observed, indicating stability of prepared
formulation. The gel developed in this study is expected to forward
the usage of M. × piperita essential towards commercial application.
Abstract: This paper presents a hybrid fuzzy-PD plus PID
(HFPP) controller and its application to steam distillation process for
essential oil extraction system. Steam temperature is one of the most
significant parameters that can influence the composition of essential
oil yield. Due to parameter variations and changes in operation
conditions during distillation, a robust steam temperature controller becomes nontrivial to avoid the degradation of essential oil quality.
Initially, the PRBS input is triggered to the system and output of steam temperature is modeled using ARX model structure. The
parameter estimation and tuning method is adopted by simulation
using HFPP controller scheme. The effectiveness and robustness of
proposed controller technique is validated by real time
implementation to the system. The performance of HFPP using 25 and 49 fuzzy rules is compared. The experimental result demonstrates the proposed HFPP using 49 fuzzy rules achieves a
better, consistent and robust controller compared to PID when considering the test on tracking the set point and the effects due to disturbance.