Interspecific Variation in Heat Stress Tolerance and Oxidative Damage among 15 C3 Species

The C3 plants are frequently suffering from exposure to high temperature stress which limits the growth and yield of these plants. This study seeks to clarify the physiological mechanisms of heat tolerance in relation to oxidative stress in C3 species. Fifteen C3 species were exposed to prolonged moderately high temperature stress 36/30°C for 40 days in a growth chamber. Chlorophyll fluorescence (Fv/Fm) showed great difference among species at 40 days of the stress. The species showed decreases in Fv/Fm and increases in malondialdehyde (MDA) content under stress condition as well as negative correlation between Fv/Fm and MDA (r = -0.61*) at 40 days of the stress. Hydrogen peroxide (H2O2) content before and after stress in addition to its response under stress showed great differences among species. The results suggest that the difference in heat tolerance among C3 species is closely associated with the ability to suppress oxidative damage but not with the content of reactive oxygen species (ROS) which is regulated by complex network.

The Tyrosinase and Cyclooxygenase Inhibitory Activities and Cytotoxicity Screening of Tamarindus indica Seeds

The methanolic extracts from seeds of tamarind (Tamarindus indica) was prepared by Soxhlet apparatus extraction and evaluated for total phenolic content by Folin-Ciocalteu method. Then, methanolic extract was screened biological activities (In vitro) for anti-melanogenic activity by tyrosinase inhibition test, antiinflammation activity by cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2) inhibition test, and cytotoxic screening test with Vero cells. The results showed that total phenolic content, which contained in extract, was contained 27.72 mg of gallic acid equivalent per g of dry weight. The ability to inhibit tyrosinase enzyme, which exerted by Tamarind seed extracts (1 mg/ml) was 52.13 ± 0.42 %. The extract was not possessed inhibitory effect to COX-1 and COX-2 enzymes and cytotoxic effect to Vero cells. The finding is concludes that tested seed extract was possessed antimelanogenic activity with non-toxic effects. However, there was not exhibited anti-inflammatory activity. Further studies include the use of advance biological models to confirm this biological activity, as well as, the isolation and characterization of the purified compounds that it was contained.

Mathematical Models for Overall Gas Transfer Coefficient Using Different Theories and Evaluating Their Measurement Accuracy

Oxygen transfer, the process by which oxygen is transferred from the gaseous to liquid phase, is a vital part of the waste water treatment process. Because of low solubility of oxygen and consequent low rate of oxygen transfer, sufficient oxygen to meet the requirement of aerobic waste does not enter through normal surface air water interface. Many theories have come up in explaining the mechanism of gas transfer and absorption of non-reacting gases in a liquid, of out of which, Two film theory is important. An exiting mathematical model determines approximate value of Overall Gas Transfer coefficient. The Overall Gas Transfer coefficient, in case of Penetration theory, is 1.13 time more than that obtained in case of Two film theory. The difference is due to the difference in assumptions in the two theories. The paper aims at development of mathematical model which determines the value of Overall Gas Transfer coefficient with greater accuracy than the existing model.

Groundwater Quality Improvement by Using Aeration and Filtration Methods

An experiment was conducted using two aeration methods (water-into-air and air-into-water) and followed by filtration processes using manganese greensand material. The properties of groundwater such as pH, dissolved oxygen, turbidity and heavy metal concentration (iron and manganese) will be assessed. The objectives of this study are i) to determine the effective aeration method and ii) to assess the effectiveness of manganese greensand as filter media in removing iron and manganese concentration in groundwater. Results showed that final pH for all samples after treatment are in range from 7.40 and 8.40. Both aeration methods increased the dissolved oxygen content. Final turbidity for groundwater samples are between 3 NTU to 29 NTU. Only three out of eight samples achieved iron concentration of 0.3mg/L and less and all samples reach manganese concentration of 0.1mg/L and less. Air-into-water aeration method gives higher percentage of iron and manganese removal compare to water-into-air method.

Biomethanation of Palm Oil Mill Effluent (POME) by Membrane Anaerobic System (MAS) using POME as a Substrate

The direct discharge of palm oil mill effluent (POME) wastewater causes serious environmental pollution due to its high chemical oxygen demand (COD) and biochemical oxygen demand (BOD). Traditional ways for POME treatment have both economical and environmental disadvantages. In this study, a membrane anaerobic system (MAS) was used as an alternative, cost effective method for treating POME. Six steady states were attained as a part of a kinetic study that considered concentration ranges of 8,220 to 15,400 mg/l for mixed liquor suspended solids (MLSS) and 6,329 to 13,244 mg/l for mixed liquor volatile suspended solids (MLVSS). Kinetic equations from Monod, Contois and Chen & Hashimoto were employed to describe the kinetics of POME treatment at organic loading rates ranging from 2 to 13 kg COD/m3/d. throughout the experiment, the removal efficiency of COD was from 94.8 to 96.5% with hydraulic retention time, HRT from 400.6 to 5.7 days. The growth yield coefficient, Y was found to be 0.62gVSS/g COD the specific microorganism decay rate was 0.21 d-1 and the methane gas yield production rate was between 0.25 l/g COD/d and 0.58 l/g COD/d. Steady state influent COD concentrations increased from 18,302 mg/l in the first steady state to 43,500 mg/l in the sixth steady state. The minimum solids retention time, which was obtained from the three kinetic models ranged from 5 to 12.3 days. The k values were in the range of 0.35 – 0.519 g COD/ g VSS • d and values were between 0.26 and 0.379 d-1. The solids retention time (SRT) decreased from 800 days to 11.6 days. The complete treatment reduced the COD content to 2279 mg/l equivalent to a reduction of 94.8% reduction from the original.

Nutrients Removal from Municipal Wastewater Treatment Plant Effluent using Eichhornia Crassipes

Water hyacinth has been used in aquatic systems for wastewater purification in many years worldwide. The role of water hyacinth (Eichhornia crassipes) species in polishing nitrate and phosphorus concentration from municipal wastewater treatment plant effluent by phytoremediation method was evaluated. The objective of this project is to determine the removal efficiency of water hyacinth in polishing nitrate and phosphorus, as well as chemical oxygen demand (COD) and ammonia. Water hyacinth is considered as the most efficient aquatic plant used in removing vast range of pollutants such as organic matters, nutrients and heavy metals. Water hyacinth, also referred as macrophytes, were cultivated in the treatment house in a reactor tank of approximately 90(L) x 40(W) x 25(H) in dimension and built with three compartments. Three water hyacinths were placed in each compartments and water sample in each compartment were collected in every two days. The plant observation was conducted by weight measurement, plant uptake and new young shoot development. Water hyacinth effectively removed approximately 49% of COD, 81% of ammonia, 67% of phosphorus and 92% of nitrate. It also showed significant growth rate at starting from day 6 with 0.33 shoot/day and they kept developing up to 0.38 shoot/day at the end of day 24. From the studies conducted, it was proved that water hyacinth is capable of polishing the effluent of municipal wastewater which contains undesirable amount of nitrate and phosphorus concentration.

Design and Control Strategy of Diffused Air Aeration System

During the past decade, pond aeration systems have been developed which will sustain large quantities of fish and invertebrate biomass. Dissolved Oxygen (DO) is considered to be among the most important water quality parameters in fish culture. Fishponds in aquaculture farms are usually located in remote areas where grid lines are at far distance. Aeration of ponds is required to prevent mortality and to intensify production, especially when feeding is practical, and in warm regions. To increase pond production it is necessary to control dissolved oxygen. Artificial intelligence (AI) techniques are becoming useful as alternate approaches to conventional techniques or as components of integrated systems. They have been used to solve complicated practical problems in various areas and are becoming more and more popular nowadays. This paper presents a new design of diffused aeration system using fuel cell as a power source. Also fuzzy logic control Technique (FLC) is used for controlling the speed of air flow rate from the blower to air piping connected to the pond by adjusting blower speed. MATLAB SIMULINK results show high performance of fuzzy logic control (FLC).

Influence of Degradative Enzymatic Activities on the Shelf Life of Ready-to-Eat Prickly Pear Fruits

Prickly pear fruit (Opuntia ficus indica L. Miller) belongs to the Cactaceae family. This species is very sensitive to low storage temperatures (< 5°C) which cause damages. The fruits can be peeled, suitably packaged and successfully commercialized as a ready-to-eat product. The main limit to the extension of the shelf life is the production of off-flavors due to different factors, the growth of microorganisms and the action of endogenous enzymes. Lipoxygenase (LOX) and Pectinesterase (PE) are involved in fruit degradation. In particular, LOX pathway is directly responsible for lipid oxidation, and the subsequent production of off-flavours, while PE causes the softening of fruit during maturation. They act on the texture and shelf-life of post-harvest, packaged fruits, as a function of the the grown of microorganisms and packaging technologies used. The aim of this work is to compare the effect of different packaging technologies on the shelf life extension of ready-to-eat prickly pear fruits with regards for the enzymes activities.

Numerical Study of Oxygen Enrichment on NO Pollution Spread in a Combustion Chamber

In this study, a 3D combustion chamber was simulated using FLUENT 6.32. Aim to obtain detailed information on combustion characteristics and _ nitrogen oxides in the furnace and the effect of oxygen enrichment in a combustion process. Oxygenenriched combustion is an effective way to reduce emissions. This paper analyzes NO emission, including thermal NO and prompt NO. Flow rate ratio of air to fuel is varied as 1.3, 3.2 and 5.1 and the oxygen enriched flow rates are 28, 54 and 68 lit/min. The 3D Reynolds Averaged Navier Stokes (RANS) equations with standard k-ε turbulence model are solved together by Fluent 6.32 software. First order upwind scheme is used to model governing equations and the SIMPLE algorithm is used as pressure velocity coupling. Results show that for AF=1.3, increase the oxygen flow rate of oxygen reduction in NO emissions is Lance. Moreover, in a fixed oxygen enrichment condition, increasing the air to fuel ratio will increase the temperature peak, but not the NO emission rate. As a result, oxygen enrichment can reduce the NO emission at this kind of furnace in low air to fuel rates.

Fenton’s Oxidation as Post-Treatment of a Mature Municipal Landfill Leachate

Mature landfill leachates contain some macromolecular organic substances that are resistant to biological degradation. Recently, Fenton-s oxidation has been investigated for chemical treatment or pre-treatment of mature landfill leachates. The aim of this study was to reduce the recalcitrant organic load still remaining after the complete treatment of a mature landfill leachate by Fenton-s oxidation post-treatment. The effect of various parameters such as H2O2 to Fe2+ molar ratio, dosage of Fe2+ reagent, initial pH, reaction time and initial chemical oxygen demand (COD) strength, that have an important role on the oxidation, was analysed. A molar ratio H2O2/Fe2+ = 3, a Fe2+ dosage of 4 mmol·L-1, pH 3, and a reaction time of 40 min were found to achieve better oxidation performances. At these favorable conditions, COD removal efficiency was 60.9% and 31.1% for initial COD of 93 and 743 mg·L-1 respectively (diluted and non diluted leachate). Fenton-s oxidation also presented good results for color removal. In spite of being extremely difficult to treat this leachate, the above results seem rather encouraging on the application of Fenton-s oxidation.

Assessment of Water Pollution of Kowsar Dam Reservoir

The reservoir of Kowsar dam supply water for different usages such as aquaculture farms , drinking, agricultural and industrial usages for some provinces in south of Iran. The Kowsar dam is located next to the city of Dehdashat in Kohgiluye and Boyerahmad province in southern Iran. There are some towns and villages on the Kowsar dam watersheds, which Dehdasht and Choram are the most important and populated twons in this area, which can to be sources of pollution for water reservoir of the Kowsar dam . This study was done to determine of water pollution of the Kowsar dam reservoir which is one of the most important water resources of Kohkiloye and Boyerahmad and Bushehr provinces in south-west Iran. In this study , water samples during 12 months were collected to examine Biochemical Oxygen Demand (BOD) and Dissolved Oxygen(DO) as a criterion for evaluation of water pollution of the reservoir. In summary ,the study has shown Maximum, average and minimum levels of BOD have observed 25.9 ,9.15 and 2.3 mg/L respectively and statistical parameters of data such as standard deviation , variance and skewness have calculated 7.88, 62 and 1.54 respectively. Finally the results were compared with Iranian national standards. Among the analyzed samples, as the maximum value of BOD (25.9 mg/L) was observed at the May 2010 , was within the maximum admissible limits by the Iranian standards.

Resistive RAM Based on Hfox and its Temperature Instability Study

High performance Resistive Random Access Memory (RRAM) based on HfOx has been prepared and its temperature instability has been investigated in this work. With increasing temperature, it is found that: leakage current at high resistance state increases, which can be explained by the higher density of traps inside dielectrics (related to trap-assistant tunneling), leading to a smaller On/Off ratio; set and reset voltages decrease, which may be attributed to the higher oxygen ion mobility, in addition to the reduced potential barrier to create / recover oxygen ions (or oxygen vacancies); temperature impact on the RRAM retention degradation is more serious than electrical bias.

Biomass and Pigment Production by Monascus during Miniaturized Submerged Culture on Adlay

Three reactor types were explored and successfully used for pigment production by Monascus: shake flasks, and shaken and stirred miniaturized reactors. Also, the use of dielectric spectroscopy for the on-line measurement of biomass levels was explored. Shake flasks gave good pigment yields, but scale up is difficult, and they cannot be automated. Shaken bioreactors were less successful with pigment production than stirred reactors. Experiments with different impeller speeds in different volumes of liquid in the reactor confirmed that this is most likely due oxygen availability. The availability of oxygen appeared to affect biomass levels less than pigment production; red pigment production in particular needed very high oxygen levels. Dielectric spectroscopy was effectively used to continuously measure biomass levels during the submerged fungal fermentation in the shaken and stirred miniaturized bioreactors, despite the presence of the solid substrate particles. Also, the capacitance signal gave useful information about the viability of the cells in the culture.

Deoxygenation of Beef Fat over Pd Supported Mesoporous TiO2 Catalyst Prepared by Single-Step Sol-Gel Process with Surfactant Template

Deoxygenation of beef fat for the production of hydrogenated biodiesel is investigated in a high pressure continuous flow fixed bed reactor over palladium-supported mesoporous titania catalyst synthesized via a combined single-step sol-gel process with surfactant-assisted templating method (SATM). The catalyst possessed a mesoporous charactheristic with high surface area and narrow pore size distribution. The main products of all Pd/TiO2 catalysts are n-heptadecane (n-C17) and n-pentadecane (n-C15) resulting from decarbonylation reaction. Pd/TiO2 catalyst synthesized via a combined single-step sol-gel process with SATM (SSSG) gave higher activity and selectivity to the desired products when compared to IWI/SG-TiO2 and IWI/P25-TiO2, respectively. SSSG catalyst gave the average conversion up to 80-90 % and 80 % for the selectivity in diesel range hydrocarbons. This result may cause by the higher surface area and the ability in dispersion of palladium ion in mesoporous of TiO2 during sol-gel process.

Induction of Alternative Oxidase Activity in Candida albicans by Oxidising Conditions

Candida albicans ATCC 10231 had low endogenous activity of the alternative oxidase compared with that of C. albicans ATCC 10261. In C. albicans ATCC 10231 the endogenous activity declined as the cultures aged. Alternative oxidase activity could be induced in C. albicans ATCC 10231 by treatment with cyanide, but the induction of this activity required the presence of oxygen which could be replaced, at least in part, with high concentrations of potassium ferricyanide. We infer from this that the expression of the gene encoding the alternative oxidase is under the control of a redoxsensitive transcription factor.

Treatment of Petroleum Refinery Wastewater by using UASB Reactors

Petroleum refineries discharged large amount of wastewater -during the refining process- that contains hazardous constituents that is hard to degrade. Anaerobic treatment process is well known as an efficient method to degrade high strength wastewaters. Up-flow Anaerobic Sludge Blanker (UASB) is a common process used for various wastewater treatments. Two UASB reactors were set up and operated in parallel to evaluate the treatment efficiency of petroleum refinery wastewater. In this study four organic volumetric loading rates were applied (i.e. 0.58, 0.89, 1.21 and 2.34 kg/m3·d), two loads to each reactor. Each load was applied for a period of 60 days for the reactor to acclimatize and reach steady state, and then the second load applied. The chemical oxygen demand (COD) removals were satisfactory with the removal efficiencies at the loadings applied were 78, 82, 83 and 81 % respectively.

General Haemodynamics, Aerobic Potential and Strategy for Adaptation of Students to Team Sports

Differentiated impact of team sports (basketball, indoor soccer, handball) on general haemodynamics and aerobic potential of students who specialize in technical subjects is detected only on the fourth year of studies in the institute of higher education. Those who play basketball and indoor soccer have shown increase of stroke and minute volume of blood indices, pumping and contractile function of the heart, oxygenation of blood and oxygen delivery to tissues, aerobic energy supply and balance of sympathetic and parasympathetic activity of the nervous regulation mechanism of the circulatory system. Those who play handball have shown these indices statistically decreased. On the whole playing basketball and indoor soccer optimizes the strategy for adaptation of students to the studying process, but playing handball does the opposite thing. The leading factor for adaptation of students is: those who play basketball have increase of minute blood volume which stipulates velocity of the system blood circulation and well-timed oxygen delivery to tissues; those who play indoor soccer have increase of power and velocity of contractile function of the heart; those who play handball have increase of resistance of thorax to the system blood flow which minimizes contractile function of the heart, blood oxygen saturation and delivery of oxygen to tissues.

Construction of Water Electrolyzer for Single Slice O2/H2 Polymer Electrolyte Membrane Fuel Cell

In the first part of the research work, an electrolyzer (10.16 cm dia and 24.13 cm height) to produce hydrogen and oxygen was constructed for single slice O2/H2 fuel cell using cation exchange membrane. The electrolyzer performance was tested with 23% NaOH, 30% NaOH, 30% KOH and 35% KOH electrolyte solution with current input 4 amp and 2.84 V from the rectifier. Rates of volume of hydrogen produced were 0.159 cm3/sec, 0.155 cm3/sec, 0.169 cm3/sec and 0.163 cm3/sec respectively from 23% NaOH, 30% NaOH, 30% KOH and 35% KOH solution. Rates of volume of oxygen produced were 0.212 cm3/sec, 0.201 cm3/sec, 0.227 cm3/sec and 0.219 cm3/sec respectively from 23% NaOH, 30% NaOH, 30% KOH and 35% KOH solution (1.5 L). In spite of being tested the increased concentration of electrolyte solution, the gas rate does not change significantly. Therefore, inexpensive 23% NaOH electrolyte solution was chosen to use as the electrolyte in the electrolyzer. In the second part of the research work, graphite serpentine flow plates, fiberglass end plates, stainless steel screen electrodes, silicone rubbers were made to assemble the single slice O2/H2 polymer electrolyte membrane fuel cell (PEMFC).