Abstract: Problem of food preservation is extremely important
for mankind. Viscous damage ("illness") of bread results from
development of Bacillus spp. bacteria. High temperature resistant
spores of this microorganism are steady against 120°C) and remain in
bread during pastries, potentially causing spoilage of the final
product. Scientists are interested in further characterization of bread
spoiling Bacillus spp. species. Our aim was to find weather yeast
Saccharomyces cerevisiae strains that are able to produce natural
antimicrobial killer factor can preserve bread illness. By diffusion
method, we showed yeast antagonistic activity against spore-forming
bacteria. Experimental technological parameters were the same as for
bakers' yeasts production on the industrial scale. Risograph test
during dough fermentation demonstrated gas production. The major
finding of the study was a clear indication of the presence of killer
yeast strain antagonistic activity against rope in bread causing
bacteria. After demonstrating antagonistic effect of S. cerevisiae on
bacteria using solid nutrient medium, we tested baked bread under
provocative conditions. We also measured formation of carbon
dioxide in the dough, dough-making duration and quality of the final
products, when using different strains of S. cerevisiae. It is
determined that the use of yeast S. cerevisiae RCAM 01730 killer
strain inhibits appearance of rope in bread. Thus, natural yeast
antimicrobial killer toxin, produced by some S. cerevisiae strains is
an anti-rope in bread protector.
Abstract: In the present research, whole meal barley flour
(WBF) was supplemented with gelatinized corn flour (GCF) in 0 and
30%. Whole meal wheat flour (WWF) was mixed with defatted rice
bran (DRB) to produce 0, 20, 25, and 30% replacement levels.
Rheological properties of dough were studied. Thermal properties
and starch crystallinity of flours were evaluated. Flat bread, balady
bread and pie were prepared from the different flour blends. The
different bakeries were sensory evaluated. Color of raw materials and
crust of bakery products were determined. Nutrients contents of raw
flours and food products were assessed. Results showed that addition
of GCF to WBF increased the viscosity and falling number of the
produced dough. Water absorption, dough development time and
dough stability increased with increasing the level of DRB in dough
while, weakening and mixing tolerance index decreased.
Extensibility and energy decreased, while, resistance to extension
increased as DRB level increased. Gelatinized temperature of WWF,
WBF, GCF, and DRB were 13.26, 35.09, 28.33, and 39.63,
respectively. Starch crystallinity was affected when DRB was added
to WWF. The highest protein content was present in balady bread
made from 70% WWF and 30% DRB. The highest calcium,
phosphorus, and potassium levels were present in products made
from 100% WBF. Sensory attributes of the products were slightly
affected by adding DRB and GCF. Conclusion: Addition of DRB or
GCF to WWF or WBF, respectively affect the physical, chemical,
rheological and sensory properties of balady bread, flat bread, and pie
while improved their nutritive values.
Abstract: Diets high in processed foods have been found to lack
essential micro-nutrients for optimum human development and
overall health. Some micro-nutrients such as copper (Cu) have been
found to enhance the inflammatory response through its oxidative
functions, thereby having a role in cardiovascular disease, metabolic
syndrome, diabetes and related complications. This research study
was designed to determine if food crops could be bio-fortified with
micro-nutrients by growing sprouts on mineral fortified fiber mats. In
the feasibility study described in this contribution, recycled cellulose
fibers and clay, saturated with either micro-nutrient copper ions or
copper nanoparticles, were converted to a novel mineral-cellulose
fiber carrier of essential micro-nutrient and of antimicrobial
properties. Seeds of Medicago sativa (alfalfa), purchased from a
commercial, organic supplier were germinated on engineered
cellulose fiber mats. After the appearance of the first leaves, the
sprouts were dehydrated and analyzed for Cu content. Nutrient
analysis showed ~2 increase in Cu of the sprouts grown on the fiber
mats with copper particles, and ~4 increase on mats with ionic copper
as compared to the control samples. This study illustrates the
potential for the use of engineered mats as a viable way to increase
the micro-nutrient composition of locally-grown food crops and the
need for additional research to determine the uptake, nutritional
implications and risks of micro-nutrient bio-fortification.
Abstract: Polycyclic Aromatic Hydrocarbons (PAHs) are
formed mainly because of incomplete combustion of organic
materials during industrial, domestic activities or natural occurrence.
Their toxicity and contamination of terrestrial and aquatic ecosystem
have been established. However, with limited validity index, previous
research has focused on PAHs isomer pair ratios of variable
physicochemical properties in source identification. The objective of
this investigation was to determine the empirical validity of Pearson
Correlation Coefficient (PCC) and Cluster Analysis (CA) in PAHs
source identification along soil samples of different land uses.
Therefore, 16 PAHs grouped, as Endocrine Disruption Substances
(EDSs) were determined in 10 sample stations in top and sub soils
seasonally. PAHs was determined the use of Varian 300 gas
chromatograph interfaced with flame ionization detector. Instruments
and reagents used are of standard and chromatographic grades
respectively. PCC and CA results showed that the classification of
PAHs along pyrolitic and petrogenic organics used in source
signature is about the predominance PAHs in environmental matrix.
Therefore, the distribution of PAHs in the studied stations revealed
the presence of trace quantities of the vast majority of the sixteen
PAHs, which may ultimately inhabit the actual source signature
authentication. Therefore, factors to be considered when evaluating
possible sources of PAHs could be; type and extent of bacterial
metabolism, transformation products/substrates, and environmental
factors such as salinity, pH, oxygen concentration, nutrients, light
intensity, temperature, co-substrates, and environmental medium are
hereby recommended as factors to be considered when evaluating
possible sources of PAHs.
Abstract: In this glasshouse study, we developed a new imagebased
non-destructive technique for detecting leaf P status of
different crops such as cotton, tomato and lettuce. The plants were
grown on a nutrient solution containing different P concentrations,
e.g. 0%, 50% and 100% of recommended P concentration (P0 = no P,
L; P1 = 2.5 mL 10 L-1 of P and P2 = 5 mL 10 L-1 of P). After 7 weeks
of treatment, the plants were harvested and data on leaf P contents
were collected using the standard destructive laboratory method and
at the same time leaf images were collected by a handheld crop image
sensor. We calculated leaf area, leaf perimeter and RGB (red, green
and blue) values of these images. These data were further used in
linear discriminant analysis (LDA) to estimate leaf P contents, which
successfully classified these plants on the basis of leaf P contents.
The data indicated that P deficiency in crop plants can be predicted
using leaf image and morphological data. Our proposed nondestructive
imaging method is precise in estimating P requirements of
different crop species.
Abstract: Household waste form a larger proportion of waste
generated across the state, accumulation of organic waste is an
apparent problem and the existing dump sites could be overstress.
Niger state has abundant arable land and water resources thus should
be one of the highest producers of agricultural crops in the country.
However, the major challenge to agricultural sector today is loss of
soil nutrient coupled with high cost of fertilizer. These have
continued to increase the use of fertilizer and decomposed solid waste
for enhance agricultural yield, which have varying effects on the soil
as well a threat to human livelihood. Consequently, vegetable yield
samples from poultry droppings, decomposed household waste
manure, NPK treatments and control from each replication were
subjected to proximate analysis to determine the nutritional and antinutritional
component as well as heavy metal concentration. Data
collected was analyzed using SPSS software and Randomized
complete Block Design means were compared. The result shows that
the treatments do not devoid the concentrations of any nutritional
components while the anti-nutritional analysis proved that NPK had
higher oxalate content than control and organic treats. The
concentration of lead and cadmium are within safe permissible level
while the mercury level exceeded the FAO/WHO maximum
permissible limit for the entire treatments depicts the need for urgent
intervention to minimize mercury levels in soil and manure in order
to mitigate its toxic effect. Thus, eco-agriculture should be widely
accepted and promoted by the stakeholders for soil amendment,
higher yield, strategies for sustainable environmental protection, food
security, poverty eradication, attainment of sustainable development
and healthy livelihood.
Abstract: This study aims at improving the urban hydrological
cycle of the Orléans agglomeration (France) and understanding the
relationship between physical and chemical parameters of urban
surface runoff and the hydrological conditions. In particular water
quality parameters such as pH, conductivity, total dissolved solids,
major dissolved cations and anions, and chemical and biological
oxygen demands were monitored for three types of urban water
discharges (wastewater treatment plant output (WWTP), storm
overflow and stormwater outfall) under two hydrologic scenarios (dry
and wet weather). The first results were obtained over a period of five
months. Each investigated (Ormes, l’Egoutier and La Corne) outfall
represents an urban runoff source that receives water from runoff
roads, gutters, the irrigation of gardens and other sources of flow over
the Earth’s surface that drains in its catchments and carries it to the
Loire River. In wet weather conditions there is rain water runoff and
an additional input from the roof gutters that have entered the
stormwater system during rainfall. For the comparison the results La
Chilesse is a storm overflow that was selected in our study as a
potential source of waste water which is located before the (WWTP). The comparison of the physical-chemical parameters (total
dissolved solids, turbidity, pH, conductivity, dissolved organic
carbon (DOC), concentration of major cations and anions) together
with the chemical oxygen demand (COD) and biological oxygen
demand (BOD) helped to characterize sources of runoff waters in the
different watersheds. It also helped to highlight the infiltration of
wastewater in some stormwater systems that reject directly in the
Loire River. The values of the conductivity measured in the outflow
of Ormes were always higher than those measured in the other two
outlets. The results showed a temporal variation for the Ormes outfall
of conductivity from 1465 μS cm-1 in the dry weather flow to 650 μS
cm-1 in the wet weather flow and also a spatial variation in the wet
weather flow from 650 μS cm-1 in the Ormes outfall to 281 μS cm-1
in L’Egouttier outfall. The ultimate BOD (BOD28) showed a
significant decrease in La Corne outfall from 181 mg L-1 in the wet
weather flow to 95 mg L-1 in the dry weather flow because of the
nutrient load that was transported by the runoff.
Abstract: Salinity is one of the major factors limiting crop
production in an arid environment. Despite its global importance
soybean production suffer the problems of salinity stress causing
damages at plant development. So it is implacable to either search for
salinity enhancement of soybean plants. Therefore, in the current
study we try to clarify the mechanism that might be involved in the
ameliorating effects of osmo-protectants such as proline and glycine
betaine as well as, compost application on soybean plants grown
under salinity stress. The experiment was conducted under
greenhouse conditions at the Graduate School of Biosphere Science
Laboratory of Hiroshima University, Japan in 2011. The experiment
was designed as a spilt-split plot based on randomized complete
block design with four replications. The treatments could be
summarized as follows; (i) salinity concentrations (0 and 15 mM), (ii)
compost treatments (0 and 24 t ha-1) and (iii) the exogenous, proline
and glycine betaine concentrations (0 mM and 25 mM) for each.
Results indicated that salinity stress induced reduction in growth and
physiological aspects (dry weight per plant, chlorophyll content, N
and K+ content) of soybean plant compared with those of the
unstressed plants. On the other hand, salinity stress led to increases in
the electrolyte leakage ratio, Na and proline contents. Special
attention was paid to, the tolerance against salt stress was observed,
the improvement of salt tolerance resulted from proline, glycine
betaine and compost were accompanied with improved K+, and
proline accumulation. While, significantly decreased electrolyte
leakage ratio and Na+ content. These results clearly demonstrate that
harmful effect of salinity could reduce on growth aspects of soybean.
Consequently, exogenous osmoprotectants combine with compost
will effectively solve seasonal salinity stress problem and are a good
strategy to increase salinity resistance of soybean in the drylands.
Abstract: Present work deals with the possible use of fertigation
in agriculture and its impact on the availability of mineral nitrogen
(Nmin) in topsoil and subsoil horizons. The aim of the present study is
to demonstrate the effect of the organic matter presence in fertigation
on microbial transformation and availability of mineral nitrogen
forms. The main investigation reason is the potential use of pretreated
waste water, as a source of organic carbon (Corg) and residual
nutrients (Nmin) for fertigation. Laboratory experiment has been
conducted to demonstrate the effect of the arable land fertilization
method on the Nmin availability in different depths of the soil with
the usage of model experimental containers filled with soil from
topsoil and podsoil horizons that were taken from the precise area.
Tufted hairgrass (Deschampsia caespitosa) has been chosen as a
model plant. The water source protection zone Brezova nad Svitavou
has been a research area where significant underground reservoirs of
drinking water of the highest quality are located. From the second
half of the last century local sources of drinking water show
nitrogenous compounds increase that get here almost only from
arable lands. Therefore, an attention of the following text focuses on
the fate of mineral nitrogen in the complex plant-soil. Research
results show that the fertigation application with Corg in a
combination with mineral fertilizer can reduce the amount of Nmin
leached from topsoil horizon of agricultural soils. In addition, some
plants biomass production reduces may occur.
Abstract: Mustard leaves are rich in folates, vitamin A, K and
B-complex. Mustard greens are low in calories and fats and rich in
dietary fiber. They are rich in potassium, manganese, iron, copper,
calcium, magnesium and low in sodium. It is very rich in antioxidants
and Phytonutrients. For the optimization of process variables
(moisture content and mustard leave powder), the experiments were
conducted according to central composite Face Centered Composite
design of RSM. The mustard leaves powder was replaced with
composite flour (a combination of rice, chickpea and corn in the ratio
of 70:15:15). The extrudate was extruded in a twin screw extruder at
a barrel temperature of 120°C. The independent variables were
mustard leaves powder (2-10 %) and moisture content (12-20 %).
Responses analyzed were bulk density, water solubility index, water
absorption index, lateral expansion, antioxidant activity, total
phenolic content, and overall acceptability. The optimum conditions
obtained were 7.19 g mustard leaves powder in 100g premix having
16.8% moisture content (w.b).
Abstract: Continuous upflow filters can combine the nutrient
(nitrogen and phosphate) and suspended solid removal in one unit
process. The contaminant removal could be achieved chemically or
biologically; in both processes the filter removal efficiency depends
on the interaction between the packed filter media and the influent. In
this paper a residence time distribution (RTD) study was carried out
to understand and compare the transfer behaviour of contaminants
through a selected filter media packed in a laboratory-scale
continuous up flow filter; the selected filter media are limestone and
white dolomite. The experimental work was conducted by injecting a
tracer (red drain dye tracer –RDD) into the filtration system and then
measuring the tracer concentration at the outflow as a function of
time; the tracer injection was applied at hydraulic loading rates
(HLRs) (3.8 to 15.2 m h-1). The results were analysed according to
the cumulative distribution function F(t) to estimate the residence
time of the tracer molecules inside the filter media. The mean
residence time (MRT) and variance σ2 are two moments of RTD that
were calculated to compare the RTD characteristics of limestone with
white dolomite. The results showed that the exit-age distribution of
the tracer looks better at HLRs (3.8 to 7.6 m h-1) and (3.8 m h-1) for
limestone and white dolomite respectively. At these HLRs the
cumulative distribution function F(t) revealed that the residence time
of the tracer inside the limestone was longer than in the white
dolomite; whereas all the tracer took 8 minutes to leave the white
dolomite at 3.8 m h-1. On the other hand, the same amount of the
tracer took 10 minutes to leave the limestone at the same HLR. In
conclusion, the determination of the optimal level of hydraulic
loading rate, which achieved the better influent distribution over the
filtration system, helps to identify the applicability of the material as
filter media. Further work will be applied to examine the efficiency
of the limestone and white dolomite for phosphate removal by
pumping a phosphate solution into the filter at HLRs (3.8 to 7.6 m h-1).
Abstract: A pilot field study was conducted at the Jagjeetpur
Municipal Sewage treatment plant situated in the Haridwar town in
Uttarakhand state, India. The objectives of the present study were to
study the effect of treated wastewater on the production of various
paddy varieties (Sharbati, PR-114, PB-1, Menaka, PB1121 and PB
1509) and the emission of GHG gases (CO2, CH4 and N2O) as
compared to the same varieties grown in the control plots irrigated
with fresh water. Of late, the concept of water footprint assessment
has emerged, which explains enumeration of various types of water
footprints of an agricultural entity from its production to processing
stages. Paddy, the most water demanding staple crop of Uttarakhand
state, displayed a high green water footprint value of 2474.12 m3/
Ton. Most of the wastewater irrigated varieties displayed up to 6%
increase in production, except Menaka and PB-1121, which showed a
reduction in production (6% and 3% respectively), due to pest and
insect infestation. The treated wastewater was observed to be rich in
Nitrogen (55.94 mg/ml Nitrate), Phosphorus (54.24 mg/ml) and
Potassium (9.78 mg/ml), thus rejuvenating the soil quality and not
requiring any external nutritional supplements. A Percentage increase
of GHG gases of irrigation with treated municipal wastewater as
compared to control plots was observed as 0.4% - 8.6% (CH4), 1.1%
- 9.2% (CO2), and 0.07% - 5.8% (N2O). The variety, Sharbati,
displayed maximum production (5.5 ton/ha) and emerged as the most
resistant variety against pests and insects. The emission values of
CH4, CO2 and N2O were 729.31 mg/m2/d, 322.10 mg/m2/d and
400.21 mg/m2/d in water stagnant condition.
This study highlighted a successful possibility of reuse of
wastewater for non-potable purposes offering the potential for
exploiting this resource that can replace or reduce the existing use of
fresh water sources in agriculture sector.
Abstract: This study was conducted to determine that carrot powder obtain by different drying methods (oven and vacuum-freeze dryer) of carrot unfit for human consumption that whether feed additives in animal nutrition or not. Carrots randomly divided 2 groups. First group was dried by using oven, second group was by using vacuum freeze dryer methods. Dried carrot prepared from fresh carrot was analysed nutrient matter (energy, crude protein, crude oil, crude ash, beta carotene, mineral concentration and colour). The differences between groups in terms of energy, crude protein, ash, Ca and Mg was not significant (P>0,05). Crude oil, P, beta carotene content and colour values (L, a, b) with vacuum-freeze dryer group was greater than oven group (P
Abstract: For this study, a town based soil database created in
Gümüsçay District of Biga Town, Çanakkale, Turkey. Crop and
livestock production are major activities in the district. Nutrient
management is mainly based on commercial fertilizer application
ignoring the livestock manure. Within the boundaries of district, 122
soil sampling points determined over the satellite image. Soil samples
collected from the determined points with the help of handheld
Global Positioning System. Labeled samples were sent to a
commercial laboratory to determine 11 soil parameters including
salinity, pH, lime, organic matter, nitrogen, phosphorus, potassium,
iron, manganese, copper and zinc. Based on the test results soil maps
for mentioned parameters were developed using remote sensing, GIS,
and geostatistical analysis. In this study we developed a GIS database
that will be used for soil nutrient management. Methods were
explained and soil maps and their interpretations were summarized in
the study.
Abstract: Whey is the lactose rich by-product of the dairy
industry, having good amount of nutrient reservoir. Most abundant
nutrients are lactose, soluble proteins, lipids and mineral salts.
Disposing of whey by most of milk plants which do not have proper
pre-treatment system is the major issue. As a result of which, there
can be significant loss of potential food and energy source. Thus,
whey has been explored as the substrate for the synthesis of different
value added products such as enzymes. β-galactosidase is one of the
important enzymes and has become the major focus of research due
to its ability to catalyze both hydrolytic as well as
transgalactosylation reaction simultaneously. The enzyme is widely
used in dairy industry as it catalyzes the transformation of lactose to
glucose and galactose, making it suitable for the lactose intolerant
people. The enzyme is intracellular in both bacteria and yeast,
whereas for molds, it has an extracellular location. The present work
was carried to utilize the whey for the production of β-galactosidase
enzyme using both yeast and fungal cultures. The yeast isolate
Kluyveromyces marxianus WIG2 and various fungal strains have
been used in the present study. Different disruption techniques have
also been investigated for the extraction of the enzyme produced
intracellularly from yeast cells. Among the different methods tested
for the disruption of yeast cells, SDS-chloroform showed the
maximum β-galactosidase activity. In case of the tested fungal
cultures, Aureobasidium pullulans NCIM 1050 was observed to be
the maximum extracellular enzyme producer.
Abstract: Optimal feeding, including optimal micronutrient
intake, becomes one of the ways to overcome the long-term
consequences of undernutrition. Macronutrient and micronutrient
intake were important to a rapid growth and development of young
children. The study objective was to assess macro and micronutrient
intake and its adequacy in children aged 12-23 months. This survey
was a cross-sectional study, involving 83 caregivers with children
aged 12-23 months old in Senen Sub-district, Central Jakarta selected
through simple random sampling. Data on nutrient intake was
obtained through interview using single 24-hour recall. Repeated 24-
hour recall to sub-sample was done to estimate the proportion of
nutrient inadequacy. The highest prevalence of nutrient inadequacy
was iron (52.4%), followed by vitamin C (30.9%) and zinc (28.8%).
Almost 12% children had inadequate energy intake. More than half
of children (62.6%) were anemic (25.3% were severely anemic).
Micronutrient inadequacy, especially iron, was more problematic
than macronutrient inadequacy in the study area.
Abstract: Extracting and determining chlorophyll pigments
(chlorophyll a and b) in green leaves are the procedures based on the
solvent extraction of pigments in samples using N,Ndimethylformamide
as the extractant. In this study, two species of
soluble inorganic selenium forms, selenite (SeIV) and selenate (SeVI)
at different concentrations were investigated on maize plants that
were growing in nutrient solutions during 2 weeks and at the end of
the experiment, amounts of chlorophyll a and b for first and second
leaves of maize were measured. In accordance with the results we
observed that our regarded Se concentrations in both forms of SeIV
and SeVI were not effective on maize plants’ chlorophyll a and b
significantly although high level of 3 mg.kg-1 SeIV had negative affect
on growth of the samples that had been treated by it but about SeVI
samples we did not observe this state and our different considered
SeVI concentrations were not toxic for maize plants.
Abstract: Advanced treatments such as forward osmosis (FO)
can be used to separate or reject nutrients from secondary treated
effluents. Forward osmosis uses the chemical potential across the
membrane, which is the osmotic pressure gradient, to induce water to
flow through the membrane from a feed solution (FS) into a draw
solution (DS). The performance of FO is affected by the membrane
characteristics, composition of the FS and DS, and operating
conditions. The aim of this study was to investigate the optimum
velocity and temperature for nutrient rejection and water flux
performance in FO treatments. MgCl2 was used as the DS in the FO
process. The results showed that higher cross flow velocities yielded
higher water fluxes. High rejection of nutrients was achieved by using
a moderate cross flow velocity at 0.25 m/s. Nutrient rejection was
insensitive to temperature variation, whereas water flux was
significantly impacted by it. A temperature of 25°C was found to be
good for nutrient rejection.
Abstract: The grain quality of chickpea in Iran is low and
instable, which may be attributed to the evolution of cultivars with a
narrow genetic base making them vulnerable to biotic stresses. Four
chickpea varieties from diverse geographic origins were chosen and
arranged in a randomized complete block design. Mesorhizobium sp.
cicer strain SW7 was added to all the chickpea seeds. Chickpea seeds
were planted on October 9, 2013. Each genotype was sown 5 m in
length, with 35 cm inter-row spacing, in 3 rows. Weeds were
removed manually in all plots. Results showed that Analysis of
variance on the studied traits showed significant differences among
genotypes for N, P, K and Fe contents of chickpea, but there is not a
significant difference among Ca, Zn and Mg continents of chickpea.
The experimental coefficient of variation (CV) varied from 7.3 to
15.8. In general, the CV value lower than 20% is considered to be
good, indicating the accuracy of conducted experiments. The highest
grain N was observed in Hashem and Jam cultivars. The highest grain
P was observed in Jam cultivar. Phosphorus content (mg/100g)
ranged from 142.3 to 302.3 with a mean value of 221.3. The negative
correlation (-0.126) was observed between the N and P of chickpea
cultivars. The highest K and Fe contents were observed in Jam
cultivar.
Abstract: Hydrothermal liquefaction (HTL) is a technique for obtaining clean biofuel from biomass in the presence of heat and pressure in an aqueous medium which leads to a decomposition of this biomass to the formation of various products. A role of operating conditions is essential for the bio-oil and other products’ yield and also quality of the products. The effects of these parameters were investigated in regards to the composition and yield of the products. Chlorellaceae microalgae were tested under different HTL conditions to clarify suitable conditions for extracting bio-oil together with value-added co-products. Firstly, different microalgae loading rates (5-30%) were tested and found that this parameter has not much significant to product yield. Therefore, 10% microalgae loading rate was selected as a proper economical solution for conditioned schedule at 250oC and 30 min-reaction time. Next, a range of temperature (210-290oC) was applied to verify the effects of each parameter by keeping the reaction time constant at 30 min. The results showed no linkage with the increase of the reaction temperature and some reactions occurred that lead to different product yields. Moreover, some nutrients found in the aqueous product are possible to be utilized for nutrient recovery.