Abstract: The present study consisted of an applied test in meat
system to assess the effectiveness of three bio agents bacteriocinproducing
strains: Lm24: Lactobacillus sakei, Lm14and Lm25:
Pediococcus spp. Two tests were carried out: The ex situ test was
intended for three batches added with crude bacteriocin solutions at
12.48 AU/ml for Lm25 and 8.4 AU/ml for Lm14 and Lm24. However, the
in situ one consisted of four batches; three of them inoculated with
one bacteriocinogenic Lm25, Lm14, Lm24, respectively. The fourth one
was used in mixture: Lm14+m24 at approximately of 107 CFU/ml. The
two used tests were done in the presence of the pathogen
St. aureus ATCC 6538, as a test strain at 103 CFU/ml. Another batch
served as a positive or a negative control was used too. The
incubation was performed at 7°C. Total viable counts, staphylococci
and lactic acid bacteria, at the beginning and at selected times with
interval of three days were enumerated. Physico-chemical
determinations (except for in situ test): pH, dry mater, sugars, fat and
total protein, at the beginning and at end of the experiment, were
done, according to the international norms. Our results confirmed the
ex situ effectiveness. Furthermore, the batches affected negatively the
total microbial load over the incubation days, and showed a
significant regression in staphylococcal load at day seven, for Lm14,
Lm24, and Lm25 of 0.73, 2.11, and 2.4 log units. It should be noticed
that, at the last day of culture, staphylococcal load was nil for the
three batches. In the in situ test, the cultures displayed less inhibitory
attitude and recorded a decrease in staphylococcal load, for Lm14,
Lm24, Lm25, Lm14+m24 of 0.73, 0.20, 0.86, 0.032 log units. Therefore,
physicochemical analysis for Lm14, Lm24, Lm25, Lm14+m24 showed an
increase in pH from 5.50 to 5.77, 6.18, 5.96, 7.22, a decrease in dry
mater from 7.30% to 7.05%, 6.87%, 6.32%, 6.00%.This result
reflects the decrease in fat ranging from 1.53% to 1.49%, 1.07%,
0.99%, 0.87%; and total protein from 6.18% to 5.25%, 5.56%,
5.37%, 5.5%. This study suggests that the use of selected strains as
Lm25 could lead to the best results and would help in preserving and
extending the shelf life of lamb meat.
Abstract: Bacteria flora of Clarias gariepinus collected from two natural habitats namely Owena River (freshwater) and Igbokoda lagoon (brackish water) were examined using standard microbiological procedures. Thirteen bacterial species were identified. The result indicated that from the identified bacteria isolated, Vibrio sp, Proteus sp. Shigella sp. and E. coli were present in both habitats (fresh and brackish waters). Others were habitat-selective such as Salmonella sp., Pseudomonas sp, Enterococcus sp, Staphylococcus sp. that were found only in freshwater habitat. While Branhamella sp, Streptococcus sp. and Micrococcus sp. were found in brackish water habitat. Bacteria load from Owena river (freshwater) was found to be the highest load recorded at 6.21 x 104cfu. T-test analysis also revealed that there was a marked significant difference between bacterial load in guts of sampled Clarias from fresh water and brackish water habitats.
Abstract: Changes in the sensory, chemical and microbiological quality of the Mediterranean hake during post-catch handling and distribution were investigated. 115 fish samples were seasonally received during three stages of the transfer route from the sea to the consumer and two storage methods were recorded, seawater and ice storage. Microbiological evaluation revealed higher status for the ice stored samples regarding heterotrophic bacteria (2.68 log cfu/g and 1.92 log cfu/g at 22oC and 37°C respectively) and psychrotrophic counts (3.20 log cfu/g), with statistically significant differences among storage methods. Sensory evaluation also revealed higher status for the ice stored samples with a mean quality index of 0.17 and a spoilage time estimated at 30 hours, in contrast to seawater storage, which varied from 0.28 to 0.3, and a 14-hour estimated spoilage. Detected pathogens were identified mainly in the seawater stored samples, posing questions on the quality of the product reaching the seafood markets.
Abstract: Microbial air contamination of the outdoor air in Marine Durres-s Harbour (Durres, Albania) was estimated by sedimentation technique in August-October 2008. The sampling areas were: Ferry Terminal (FT), Fishery Harbor (FH), East Zone (EZ), Fuel Quay (FQ) and Apollonian Beach (AB). The aim of this study was to measure the number of aerobic plate count (mesophilic aerobic bacteria) and fungi (yeasts and molds) in the outdoor air in these areas. The number of colonies that were formed determines the number of cells at the moment in the outdoor air; respectively the number of mesophilic aerobic bacteria and yeasts and molds. The measure of bacteria and fungi used is CFU (Colony Forming Units) per Petri dish. It is said that marine harbours are very polluted areas. The aim of study was the definition of mesophilic aerobic bacteria and yeasts and molds number, and the comparison of microorganisms number in air sampling areas.
Abstract: This study was carried out to reveal the bacterial composition of aerosol in the studied abattoirs. Bacteria isolated were characterized according to microbiological standards. Factors such as temperature and distance were considered as variable in this study. The isolation was carried out at different temperatures such as 27oC, 31oC and 29oC and at various distances of 100meters and 200meters away from the slaughter sites. Result obtained showed that strains of Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Lactobacillus alimentarius and Micrococcus sp. were identified. The total viable counts showed that more microorganisms were present in the morning while the least viable count of 388cfu was recorded in the evening period of this study. This study also showed that more microbial loads were recorded the further the distance is to the slaughter site. Conclusively, the array of bacteria isolated suggests that abattoir sites may be a potential source of pathogenic organisms to commuters if located within residential environment.
Abstract: An experiment was conducted in October 2008 due the ability replacement plant associate biofertilizers by chemical fertilizers and the qualifying rate of chemical N fertilizers at the moment of using this biofertilizers and the interaction of this biofertilizer on each other. This field experiment has been done in Persepolis (Throne of Jamshid) and arrange by using factorial with the basis of randomized complete block design, in three replication Azespirilium SP bacteria has been admixed with consistence 108 cfu/g and inoculated with seeds of wheat, The streptomyces SP has been used in amount of 550 gr/ha and concatenated on clay and for the qualifying range of chemical fertilizer 4 level of N chemical fertilizer from the source of urea (N0=0, N1=60, N2=120, N3=180) has been used in this experiment. The results indicated there were Significant differences between levels of Nitrogen fertilizer in the entire characteristic which has been measured in this experiment. The admixed Azespirilium SP showed significant differences between their levels in the characteristics such as No. of fertile ear, No. of grain per ear, grain yield, grain protein percentage, leaf area index and the agronomic fertilizer use efficiency. Due the interaction streptomyses with Azespirilium SP bacteria this actinomycet didn-t show any statistically significant differences between it levels.
Abstract: The effects of commercial or bovine yeasts on the
performance and blood variables of broiler chickens intoxicated with
aflatoxin were investigated in broilers. Four hundred eighty broilers
(Arbor Acres; 3-wk-old) were randomly assigned to 4 groups. Each
group (120 broiler chickens) was further randomly divided into 6
replicates of 20 chickens. The treatments were control diet without
additives (treatment 1), 250 ppb AFB1 (treatment 2), commercial
yeast, Saccharomyces cerevisiae, (CY 2.5 x 107 CFU/g) + 250 ppb
AFB1 (treatment 3) and bovine yeast, Saccharomyces cerevisiae,
(BY 2.5 x 107 CFU/g + 250 ppb AFB1 (treatment 4). Complete
randomized design (CRD) was used in the experiment. Feed
consumption and body weight were recorded at every five-day
period. On day 42, carcass compositions were determined from 30
birds per treatment. While chicks were sacrificed, 3-4 ml blood
sample was taken and stored frozen at (-20°C) for serum chemical
analysis to determine effects of consumption of diets on blood
chemistry (total protein, albumin, glucose, urea, cholesterol and
triglycerides). There were no significant differences in ADFI among
the treatments(P>0.05). However, BWG, FCR and mortality were
highly significantly different (P
Abstract: Sediment and mangrove root samples from Iko River
Estuary, Nigeria were analyzed for microbial and polycyclic
aromatic hydrocarbon (PAH) content. The total heterotrophic
bacterial (THB) count ranged from 1.1x107 to 5.1 x107 cfu/g, total
fungal (TF) count ranged from 1.0x106 to 2.7x106 cfu/g, total
coliform (TC) count ranged from 2.0x104 to 8.0x104cfu/g while
hydrocarbon utilizing bacterial (HUB) count ranged from 1.0x 105 to
5.0 x 105cfu/g. There was a range of positive correlation (r = 0.72 to
0.93) between THB count and total HUB count, respectively. The
organisms were Staphylococcus aureus, Bacillus cereus,
Flavobacterium breve, Pseudomonas aeruginosa, Erwinia
amylovora, Escherichia coli, Enterobacter sp, Desulfovibrio sp,
Acinetobacter iwoffii, Chromobacterium violaceum, Micrococcus
sedentarius, Corynebacterium sp, and Pseudomonas putrefaciens.
The PAH were Naphthalene, 2-Methylnaphthalene, Acenapthylene,
Acenaphthene, Fluorene, Phenanthene, Anthracene, Fluoranthene,
Pyrene, Benzo(a)anthracene, Chrysene, Benzo(b)fluoranthene,
Benzo(k)fluoranthene, Benzo(a)pyrene, Dibenzo(a,h)anthracene,
Benzo(g,h,l)perylene ,Indeno(1,2,3-d)pyrene with individual PAH
concentrations that ranged from 0.20mg/kg to 1.02mg/kg, 0.20mg/kg
to 1.07mg/kg and 0.2mg/kg to 4.43mg/kg in the benthic sediment,
epipellic sediment and mangrove roots, respectively. Total PAH
ranged from 6.30 to 9.93mg/kg, 6.30 to 9.13mg/kg and 9.66 to
16.68mg/kg in the benthic sediment, epipellic sediment and
mangrove roots, respectively. The high concentrations in the
mangrove roots are indicative of bioaccumulation of the pollutant in
the plant tissue. The microorganisms are of ecological significance
and the detectable quantities of polycyclic aromatic hydrocarbon
could be partitioned and accumulated in tissues of infaunal and
epifaunal organisms in the study area.
Abstract: Processing tabah bamboo shoot as fermented pickle is
one of the way to increase the shelf life of this bamboo shoot. The
advantage of this shoot is low concentration of hydro cyanic acid
(HCN) make it potential for functional food product. This study
aimed to determine the characteristic of tabah bamboo shoot pickle
such as total of lactic acid bacteria (LAB), pH, total acidity, and
hydro cyanic acid (HCN) content, and also find the LAB’s type
involved during fermentation, and organic acids’ profiles. The pickle
was made by natural fermentation with 6% salt concentration and
fermentation conducted for 13 days.
The result showed during the fermentation time, in the 4th day
LAB’s number was highest as much as 72 x 107 CFU/ml and the
lowest pH was 3.09. We also found decreasing in HCN from 37.8
ppm at the beginning to 20.52 ppm at the end of fermentation
process. The organic acids detected during the fermentation were
lactic acid with the highest concentration was 0.0546 g/100 g and
small amount of acetic acid. By using PCR method, the 18 of LABs
which had rod shape were detected as member of Lactobacillus spp.,
in which 17 strains detected as L. plantarum.
Abstract: The present study addresses problems and solutions
related to new functional food production. Wheat (Triticum aestivum
L) bran obtained from industrial mill company “Dobeles
dzirnavieks”, was used to investigate them as raw material like
nutrients for Bifidobacterium lactis Bb-12. Enzymatic hydrolysis of
wheat bran starch was carried out by α-amylase from Bacillus
amyloliquefaciens (Sigma Aldrich). The Viscozyme L purchased
from (Sigma Aldrich) were used for reducing released sugar.
Bifidibacterium lactis Bb-12 purchased from (Probio-Tec® CHR
Hansen) was cultivated in enzymatically hydrolysed wheat bran
mash. All procedures ensured the number of active Bifidobacterium
lactis Bb-12 in the final product reached 105 CFUg-1. After enzymatic
and bacterial fermentations sample were freeze dried for analysis of
chemical compounds. All experiments were performed at Faculty of
Food Technology of Latvia University of Agriculture in January-
March 2013. The obtained results show that both types of wheat bran
(enzymatically treated and non-treated) influenced the fermentative
activity and number of Bifidibacterium lactis Bb-12 viable in wheat
bran mash. Amount of acidity strongly increase during the wheat
bran mash fermentation. The main objective of this work was to
create low-energy functional enzymatically and bacterially treated
food from wheat bran using enzymatic hydrolysis of carbohydrates
and following cultivation of Bifidobacterium lactis Bb-12.
Abstract: The objectives were to identify cyanide-degrading
bacteria and study cyanide removal efficiency. Agrobacterium
tumefaciens SUTS 1 was isolated. This is a new strain of
microorganisms for cyanide degradation. The maximum growth rate
of SUTS 1 obtained 4.7 × 108 CFU/ml within 4 days. The cyanide
removal efficiency was studied at 25, 50, and 150 mg/L cyanide. The
residual cyanide, ammonia, nitrate, nitrite, pH, and cell counts were
analyzed. At 25 and 50 mg/L cyanide, SUTS 1 obtained similar
removal efficiency approximately 87.50%. At 150 mg/L cyanide,
SUTS 1 enhanced the cyanide removal efficiency up to 97.90%. Cell
counts of SUTS 1 increased when the cyanide concentration was set
at lower. The ammonia increased when the removal efficiency
increased. The nitrate increased when the ammonia decreased but the
nitrite did not detect in all experiments. pH values also increased
when the cyanide concentrations were set at higher.
Abstract: An implant elicits a biological response in the
surrounding tissue which determines the acceptance and long-term
function of the implant. Dental implants have become one of the
main therapy methods in clinic after teeth lose. A successful implant
is in contact with bone and soft tissue represent by fibroblasts. In our
study we focused on the interaction between six different chemically
and physically modified titanium implants (Tis-MALP, Tis-O, Tis-
OA, Tis-OPAAE, Tis-OZ, Tis-OPAE) with alveolar fibroblasts as
well as with five type of microorganisms (S. epidermis, S.mutans, S.
gordonii, S. intermedius, C.albicans). The analysis of microorganism
adhesion was determined by CFU (colony forming unite) and biofilm
formation. The presence of α3β1 and vinculin expression on alveolar
fibroblasts was demonstrated using phospho specific cell based
ELISA (PACE). Alveolar fibroblasts have the highest expression of
these proteins on Tis-OPAAE and Tis-OPAE. It corresponds with
results from bacterial adhesion and biofilm formation and it was
related to the lowest production of collagen I by alveolar fibroblasts
on Tis-OPAAE titanium disc.
Abstract: Lactic acid alone and its combined application with
nisin were evaluated for reducing population of naturally occurring
microorganisms on chilled shrimp. Fresh shrimps were dipped in 0,
1.0% and 2.0% (v/v) lactic acid alone and their combined application
with 0.04 (g/L/kg) nisin solution for 10 min. Total plate counts of
aerobic bacteria (TPCs), Psychrotrophic counts, population of
Pseudomonas spp., H2S producing bacteria and Lactic acid bacteria
(LAB) on shrimps were determined during storage at 4 °C. The
results indicated that total plate counts were 2.91 and 2.63 log CFU/g
higher on untreated shrimps after 7 and 14 days of storage,
respectively, than on shrimps treated with 2.0% lactic acid combined
with 0.04 (g/L/kg) nisin. Both concentrations of lactic acid indicated
significant reduction on Pseudomonas counts during storage, while
2.0% lactic acid combined with nisin indicated the highest reduction.
In addition, H2S producing bacteria were more sensitive to high
concentration of lactic acid combined with nisin during storage.
Abstract: In this paper we evaluated the efficacy of
photodynamic treatment of infected wounds on pig animal model by
diffuse reflectance spectrometry. The study was conducted on fifteen
wounds contaminated with Staphylococcus aureus bacteria that were
incubated for 30 min with methylene blue solution (c = 3.3 x 10-3 M)
and exposed to laser radiations (λ = 670 nm, P = 15 mW) for 15 min.
The efficiency of photodynamic inactivation of bacteria was
evaluated by microbiological exams and diffuse reflectance
spectrometry. The results of the microbiological exams showed that
the bacterial concentration has decreased from 6.93±0.138
logCFU/ml to 3.12±0.108 logCFU/ml. The spectral examination
showed that the diffuse reflectance of wounds contaminated with
Staphylococcus aureus has decreased from 5.06±0.036 % to
3.36±0.025 %. In conclusion, photodynamic therapy is an effective
method for the treatment of infected wounds and there is a correlation
between the CFU count and diffuse reflectance.
Abstract: White scar oyster (Crassostrea belcheri) is often eaten
raw and being the leading vehicle for foodborne disease, especially
Salmonella Weltevreden which exposed the prominent and most
resistant to radiation. Gamma irradiation at a low dose of 1 kGy was
enough to eliminate S. Weltevreden contaminated in oyster meat at a
level up to 5 log CFU/g while it still retain the raw characteristics and
equivalent sensory quality as the non-irradiated one. Process
development of ready-to-eat chilled oyster meat was conducted by
shucking the meat, individually packed in plastic bags, subjected to 1
kGy gamma radiation at chilled condition and then stored in 4oC
refrigerated temperature. Microbiological determination showed the
absence of S. Weltevreden (5 log CFU/g initial inoculated) along the
whole storage time of 30 days. Sensory evaluation indicated the
decreasing in sensory scores along storage time which determining
the product shelf life to be 18 days compared to 15 days of nonirradiated
one. The most advantage of developed process was to
provide the safe raw oyster to consumers and in addition sensory
quality retained and 3-day extension shelf life also exist.
Abstract: In the present communication, we have proposed
some new generalized measure of fuzzy entropy based upon real
parameters, discussed their and desirable properties, and presented
these measures graphically. An important property, that is,
monotonicity of the proposed measures has also been studied.
Abstract: The microbiological and physicochemical
characteristics of wetland soils in Eket Local Government Area were
studied between May 2001 and June 2003. Total heterotrophic
bacterial counts (THBC), total fungal counts (TFC), and total
actinomycetes counts (TAC) were determined from soil samples
taken from four locations at two depths in the wet and dry seasons.
Microbial isolates were characterized and identified. Particle size and
chemical parameters were also determined using standard methods.
THBC ranged from 5.2 (+0.17) x106 to 1.7 (+0.18) x107 cfu/g and
from 2.4 (+0.02) x106 to 1.4 (+0.04) x107cfu/g in the wet and dry
seasons, respectively. TFC ranged from 1.8 (+0.03) x106 to 6.6 (+
0.18) x106 cfu/g and from 1.0 (+0.04) x106 to 4.2 (+ 0.01) x106 cfu/g
in the wet and dry seasons, respectively .TAC ranged from 1.2
(+0.53) x106 to 6.0 (+0.05) x106 cfu/g and from 0.6 (+0.01) x106 to
3.2 (+ 0.12) x106 cfu/g in the wet and dry season, respectively.
Acinetobacter, Alcaligenes, Arthrobacter, Bacillus, Beijerinckja,
Enterobacter, Micrococcus, Flavobacterium, Serratia, Enterococcus,
and Pseudomonas species were predominant bacteria while
Aspergillus, Fusarium, Mucor, Penicillium, and Rhizopus were the
dominant fungal genera isolated. Streptomyces and Norcadia were
the actinomycetes genera isolated. The particle size analysis showed
high sand fraction but low silt and clay. The pH and % organic
matter were generally acidic and low, respectively at all locations.
Calcium dominated the exchangeable bases with low electrical
conductivity and micronutrients. These results provide the baseline
data of Eket wetland soils for its management for sustainable
agriculture.