Abstract: The effect of calcium salts on the storage stability and on the quality attributes of both fresh and processed product (guava powder) of white flesh guavas (var ‘Allahabad safeda’) was studied. The pulp behavioral studies of fully ripened guava fruits indicated that fruits pretreated with 3% and 4.5% calcium chloride had the least viscosity. The guava pulp powder using spray drying technique was developed and its storage stability and the moisture sorption studies were carried out for product quality evaluation at normal storage condition (27°C; 65%RH). Results revealed that powder obtained from 3% calcium chloride pretreated guavas was found to be at par with the powder obtained from control guavas after 90 days of normal storage. Studies on microbiological quality of guava pulp powder indicated that among the treatments powder obtained from guava fruit pretreated with 3% calcium chloride to be the most effective through restricting microbial counts of total plate count, yeast, mold, Staphylococcus and E. coli below their permissible limit. Moisture sorption studies of guava powder revealed that foil laminate 12μm PET/9 μm foil/38-40 μm is the most suitable packaging material recommended.
Abstract: The study explores the hybrid combination of Hydrodynamic Cavitation (HC) and Subcritical Wet Air Oxidation-based pretreatment of complex industrial effluent to enhance the biodegradability selectively (without major COD destruction) to facilitate subsequent enhanced downstream processing via anaerobic or aerobic biological treatment. Advanced oxidation based techniques can be less efficient as standalone options and a hybrid approach by combining Hydrodynamic Cavitation (HC), and Wet Air Oxidation (WAO) can lead to a synergistic effect since both the options are based on common free radical mechanism. The HC can be used for initial turbulence and generation of hotspots which can begin the free radical attack and this agitating mixture then can be subjected to less intense WAO since initial heat (to raise the activation energy) can be taken care by HC alone. Lab-scale venturi-based hydrodynamic cavitation and wet air oxidation reactor with biomethanated distillery wastewater (BMDWW) as a model effluent was examined for establishing the proof-of-concept. The results indicated that for a desirable biodegradability index (BOD: COD - BI) enhancement (up to 0.4), the Cavitation (standalone) pretreatment condition was: 5 bar and 88 min reaction time with a COD reduction of 36 % and BI enhancement of up to 0.27 (initial BI - 0.17). The optimum WAO condition (standalone) was: 150oC, 6 bar and 30 minutes with 31% COD reduction and 0.33 BI. The hybrid pretreatment (combined Cavitation + WAO) worked out to be 23.18 min HC (at 5 bar) followed by 30 min WAO at 150oC, 6 bar, at which around 50% COD was retained yielding a BI of 0.55. FTIR & NMR analysis of pretreated effluent indicated dissociation and/or reorientation of complex organic compounds in untreated effluent to simpler organic compounds post-pretreatment.
Abstract: Environment free of pollutants should be the concern of every individual but with industrialization and urbanization it is difficult to achieve. In view of achieving a pollution limited environment at low cost, a study was conducted on the use of bioremediation technology to remediate hydrocarbons and three heavy metals namely; copper (Cu), zinc (Zn) and iron (Fe) from a typical petroleum refinery wastewater in a closed system. Physicochemical and microbiological characteristics on the wastewater sample revealed that it was polluted with the aforementioned pollutants. Isolation and identification of microorganisms present in the wastewater sample revealed the presence of Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus and Staphylococcus epidermidis. Bioremediation experiments carried out on five batch reactors with different compositions but at same environmental conditions revealed that treatment T5 (boosted with the association of Bacillus subtilis, Micrococcus luteus) gave the best result in terms of oil and grease content removal (i.e. 67% in 63 days). In addition, these microorganisms were able of reducing the concentrations of heavy metals in the sample. Treatments T5, T3 (boosted with Bacillus subtilis only) and T4 (boosted with Micrococcus luteus only) gave optimum percentage uptakes of 65, 75 and 25 for Cu, Zn and Fe respectively.
Abstract: The study assessed the effect of crude oil applied at rates, 0, 2, 5, and 10% on the fractional chemical forms and availability of some metals in soils from Usen, Edo State, with no known crude oil contamination and soil from a crude oil spill site in Ubeji, Delta State, Nigeria. Three methods were used to determine the bioavailability of metals in the soils: maize (Zea mays) plant, EDTA and BCR sequential extraction. The sequential extract acid soluble fraction of the BCR extraction (most labile fraction of the soils, normally associated with bioavailability) were compared with total metal concentration in maize seedlings as a means to compare the chemical and biological measures of bioavailability. Total Fe was higher in comparison to other metals for the crude oil contaminated soils. The metal concentrations were below the limits of 4.7% Fe, 190mg/kg Cu and 720mg/kg Zn intervention values and 36mg/kg Cu and 140mg/kg Zn target values for soils provided by the Department of Petroleum Resources (DPR) guidelines. The concentration of the metals in maize seedlings increased with increasing rates of crude oil contamination. Comparison of the metal concentrations in maize seedlings with EDTA extractable concentrations showed that EDTA extracted more metals than maize plant.
Abstract: The paper presents the results of the molecular
genetics analysis in sports research, with special emphasis to use
genetic information in diagnosing of motoric predispositions in Roma
boys from East Slovakia. The ability and move are the basic
characteristics of all living organisms. The phenotypes are influenced
by a combination of genetic and environmental factors. Genetic tests
differ in principle from the traditional motoric tests, because
the DNA of an individual does not change during life. The aim of
the presented study was to examine motion abilities and to determine
the frequency of ACTN3 (R577X) gene in Roma children. Genotype
data were obtained from 138 Roma and 155 Slovak boys from 7 to 15
years old. Children were investigated on physical performance level
in association with their genotype. Biological material for genetic
analyses comprised samples of buccal swabs. Genotypes were
determined using Real Time High resolution melting PCR method
(Rotor-Gene 6000 Corbett and Light Cycler 480 Roche). The
software allows creating reports of any analysis, where information
of the specific analysis, normalized and differential graphs and many
information of the samples are shown. Roma children of analyzed
group legged to non-Romany children at the same age in all the
compared tests. The % distribution of R and X alleles in Roma
children was different from controls. The frequency of XX genotype
was 9.26%, RX 46.33% and RR was 44.41%. The frequency of XX
genotype was 9.26% which is comparable to a frequency of an Indian
population. Data were analyzed with the ANOVA test.
Abstract: This research focuses on assessing the ground water quality of Northern Lebanon affected by saline water intrusion. The chemical, physical and microbiological parameters were collected in various seasons spanning over the period of two years. Results were assessed using Geographic Information System (GIS) due to its visual capabilities in presenting the pollution extent in the studied region. Future projections of the excessive pumping were also simulated using GIS in order to assess the extent of the problem of saline intrusion in the near future.
Abstract: Not all proteins have the same nutritional value, since
protein quality strongly depends on its amino acid composition and
digestibility. The meat of game animals could be a high protein
source because of its well-balanced essential amino acids
composition. Investigations about biochemical composition of game
meat such as wild boar (Sus scrofa scrofa), roe deer (Capreolus
capreolus) and beaver (Castor fiber) are not very much. Therefore,
the aim of the investigation was evaluate protein composition of
game meat hunted in Latvia. The biochemical analysis, evaluation of
connective tissue and essential amino acids in meat samples were
done, the amino acids score were calculate. Results of analysis
showed that protein content 20.88-22.05% of all types of meat
samples is not different statistically. The content of connective tissue
from 1.3% in roe deer till 1.5% in beaver meat allowed classified
game animal as high quality meat. The sum of essential amino acids
in game meat samples were determined 7.05–8.26g100g-1. Roe deer
meat has highest protein content and lowest content of connective
tissues among game meat hunted in Latvia. Concluded that amino
acid score for limiting amino acids phenylalanine and tyrosine is high
and shows high biological value of game meat.
Abstract: Problem of food preservation is extremely important
for mankind. Viscous damage ("illness") of bread results from
development of Bacillus spp. bacteria. High temperature resistant
spores of this microorganism are steady against 120°C) and remain in
bread during pastries, potentially causing spoilage of the final
product. Scientists are interested in further characterization of bread
spoiling Bacillus spp. species. Our aim was to find weather yeast
Saccharomyces cerevisiae strains that are able to produce natural
antimicrobial killer factor can preserve bread illness. By diffusion
method, we showed yeast antagonistic activity against spore-forming
bacteria. Experimental technological parameters were the same as for
bakers' yeasts production on the industrial scale. Risograph test
during dough fermentation demonstrated gas production. The major
finding of the study was a clear indication of the presence of killer
yeast strain antagonistic activity against rope in bread causing
bacteria. After demonstrating antagonistic effect of S. cerevisiae on
bacteria using solid nutrient medium, we tested baked bread under
provocative conditions. We also measured formation of carbon
dioxide in the dough, dough-making duration and quality of the final
products, when using different strains of S. cerevisiae. It is
determined that the use of yeast S. cerevisiae RCAM 01730 killer
strain inhibits appearance of rope in bread. Thus, natural yeast
antimicrobial killer toxin, produced by some S. cerevisiae strains is
an anti-rope in bread protector.
Abstract: MicroRNAs are small non-coding RNA found in
many different species. They play crucial roles in cancer such as
biological processes of apoptosis and proliferation. The identification
of microRNA-target genes can be an essential first step towards to
reveal the role of microRNA in various cancer types. In this paper,
we predict miRNA-target genes for lung cancer by integrating
prediction scores from miRanda and PITA algorithms used as a
feature vector of miRNA-target interaction. Then, machine-learning
algorithms were implemented for making a final prediction. The
approach developed in this study should be of value for future studies
into understanding the role of miRNAs in molecular mechanisms
enabling lung cancer formation.
Abstract: Growth and remodeling of biological structures have
gained lots of attention over the past decades. Determining the
response of living tissues to mechanical loads is necessary for a wide
range of developing fields such as prosthetics design or computerassisted
surgical interventions. It is a well-known fact that biological
structures are never stress-free, even when externally unloaded. The
exact origin of these residual stresses is not clear, but theoretically,
growth is one of the main sources. Extracting body organ’s shapes
from medical imaging does not produce any information regarding
the existing residual stresses in that organ. The simplest cause of such
stresses is gravity since an organ grows under its influence from
birth. Ignoring such residual stresses might cause erroneous results in
numerical simulations. Accounting for residual stresses due to tissue
growth can improve the accuracy of mechanical analysis results. This
paper presents an original computational framework based on gradual
growth to determine the residual stresses due to growth. To illustrate
the method, we apply it to a finite element model of a healthy human
face reconstructed from medical images. The distribution of residual
stress in facial tissues is computed, which can overcome the effect of
gravity and maintain tissues firmness. Our assumption is that tissue
wrinkles caused by aging could be a consequence of decreasing
residual stress and thus not counteracting gravity. Taking into
account these stresses seems therefore extremely important in
maxillofacial surgery. It would indeed help surgeons to estimate
tissues changes after surgery.
Abstract: Magnetic Resonance Imaging Contrast Agents
(MRI-CM) are significant in the clinical and biological imaging as
they have the ability to alter the normal tissue contrast, thereby
affecting the signal intensity to enhance the visibility and detectability
of images. Superparamagnetic Iron Oxide (SPIO) nanoparticles,
coated with dextran or carboxydextran are currently available for
clinical MR imaging of the liver. Most SPIO contrast agents are
T2 shortening agents and Resovist (Ferucarbotran) is one of a
clinically tested, organ-specific, SPIO agent which has a low
molecular carboxydextran coating. The enhancement effect of
Resovist depends on its relaxivity which in turn depends on factors
like magnetic field strength, concentrations, nanoparticle properties,
pH and temperature. Therefore, this study was conducted to
investigate the impact of field strength and different contrast
concentrations on enhancement effects of Resovist. The study
explored the MRI signal intensity of Resovist in the physiological
range of plasma from T2-weighted spin echo sequence at three
magnetic field strengths: 0.47 T (r1=15, r2=101), 1.5 T (r1=7.4,
r2=95), and 3 T (r1=3.3, r2=160) and the range of contrast
concentrations by a mathematical simulation. Relaxivities of r1 and r2
(L mmol-1 Sec-1) were obtained from a previous study and the selected
concentrations were 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5,
0.6, 0.7, 0.8, 0.9, 1.0, 2.0, and 3.0 mmol/L. T2-weighted images were
simulated using TR/TE ratio as 2000 ms /100 ms. According to the
reference literature, with increasing magnetic field strengths, the
r1 relaxivity tends to decrease while the r2 did not show any
systematic relationship with the selected field strengths. In parallel,
this study results revealed that the signal intensity of Resovist at lower
concentrations tends to increase than the higher concentrations. The
highest reported signal intensity was observed in the low field strength
of 0.47 T. The maximum signal intensities for 0.47 T, 1.5 T and 3 T
were found at the concentration levels of 0.05, 0.06 and 0.05 mmol/L,
respectively. Furthermore, it was revealed that, the concentrations
higher than the above, the signal intensity was decreased
exponentially. An inverse relationship can be found between the field
strength and T2 relaxation time, whereas, the field strength was
increased, T2 relaxation time was decreased accordingly. However,
resulted T2 relaxation time was not significantly different between
0.47 T and 1.5 T in this study. Moreover, a linear correlation of
transverse relaxation rates (1/T2, s–1) with the concentrations of
Resovist can be observed. According to these results, it can conclude
that the concentration of SPIO nanoparticle contrast agents and the
field strengths of MRI are two important parameters which can affect the signal intensity of T2-weighted SE sequence. Therefore, when MR
imaging those two parameters should be considered prudently.
Abstract: Landfill waste is a common problem as it has an
economic and environmental impact even if it is closed. Landfill
waste contains a high density of various persistent compounds such
as heavy metals, organic and inorganic materials. As persistent
compounds are slowly-degradable or even non-degradable in the
environment, they often produce sublethal or even lethal effects on
aquatic organisms. The aims of the present study were to estimate
sublethal effects of the Kairiai landfill (WGS: 55°55‘46.74“,
23°23‘28.4“) leachate on the locomotor activity of rainbow trout
Oncorhynchus mykiss juveniles using the original system package
developed in our laboratory for automated monitoring, recording and
analysis of aquatic organisms’ activity, and to determine patterns of
fish behavioral response to sublethal effects of leachate. Four
different concentrations of leachate were chosen: 0.125; 0.25; 0.5 and
1.0 mL/L (0.0025; 0.005; 0.01 and 0.002 as part of 96-hour LC50,
respectively). Locomotor activity was measured after 5, 10 and 30
minutes of exposure during 1-minute test-periods of each fish (7 fish
per treatment). The threshold-effect-concentration amounted to 0.18
mL/L (0.0036 parts of 96-hour LC50). This concentration was found
to be even 2.8-fold lower than the concentration generally assumed to
be “safe” for fish. At higher concentrations, the landfill leachate
solution elicited behavioral response of test fish to sublethal levels of
pollutants. The ability of the rainbow trout to detect and avoid
contaminants occurred after 5 minutes of exposure. The intensity of
locomotor activity reached a peak within 10 minutes, evidently
decreasing after 30 minutes. This could be explained by the
physiological and biochemical adaptation of fish to altered
environmental conditions. It has been established that the locomotor
activity of juvenile trout depends on leachate concentration and
exposure duration. Modeling of these parameters showed that the
activity of juveniles increased at higher leachate concentrations, but
slightly decreased with the increasing exposure duration. Experiment
results confirm that the behavior of rainbow trout juveniles is a
sensitive and rapid biomarker that can be used in combination with
the system for fish behavior monitoring, registration and analysis to
determine sublethal concentrations of pollutants in ambient water.
Further research should be focused on software improvement aimed
to include more parameters of aquatic organisms’ behavior and to
investigate the most rapid and appropriate behavioral responses in
different species. In practice, this study could be the basis for the
development and creation of biological early-warning systems
(BEWS).
Abstract: River Hindon is an important river catering the
demand of highly populated rural and industrial cluster of western
Uttar Pradesh, India. Water quality of river Hindon is deteriorating at
an alarming rate due to various industrial, municipal and agricultural
activities. The present study aimed at identifying the pollution
sources and quantifying the degree to which these sources are
responsible for the deteriorating water quality of the river. Various
water quality parameters, like pH, temperature, electrical
conductivity, total dissolved solids, total hardness, calcium, chloride,
nitrate, sulphate, biological oxygen demand, chemical oxygen
demand, and total alkalinity were assessed. Water quality data
obtained from eight study sites for one year has been subjected to the
two multivariate techniques, namely, principal component analysis
and cluster analysis. Principal component analysis was applied with
the aim to find out spatial variability and to identify the sources
responsible for the water quality of the river. Three Varifactors were
obtained after varimax rotation of initial principal components using
principal component analysis. Cluster analysis was carried out to
classify sampling stations of certain similarity, which grouped eight
different sites into two clusters. The study reveals that the
anthropogenic influence (municipal, industrial, waste water and
agricultural runoff) was the major source of river water pollution.
Thus, this study illustrates the utility of multivariate statistical
techniques for analysis and elucidation of multifaceted data sets,
recognition of pollution sources/factors and understanding
temporal/spatial variations in water quality for effective river water
quality management.
Abstract: Strong anthropogenic impact has uncontrolled
consequences on the nature of the soil. Hence, up-to-date sustainable
methods of soil state improvement are essential. Investigators provide
the evidence that biochar can positively effects physical, chemical,
and biological soil properties and the abundance of mycorrhizal fungi
which are in the focus of this study. The main aim of the present
investigation is to demonstrate the effect of two types of plant growth
promoting bacteria (PGPB) inoculums along with the beech wood
biochar and mineral N additives on mycorrhizal colonization.
Experiment has been set up in laboratory conditions with containers
filled with arable soil from the protection zone of the main water
source “Brezova nad Svitavou”. Lactuca sativa (lettuce) has been
selected as a model plant. Based on the obtained data, it can be
concluded that mycorrhizal colonization increased as the result of
combined influence of biochar and PGPB inoculums amendment. In
addition, correlation analyses showed that the numbers of main
groups of cultivated bacteria were dependent on the degree of
mycorrhizal colonization.
Abstract: The main purpose of this study is to assess the
sediment quality and potential ecological risk in marine sediments in
Gymea Bay located in south Sydney, Australia. A total of 32 surface
sediment samples were collected from the bay. Current track
trajectories and velocities have also been measured in the bay. The
resultant trace elements were compared with the adverse biological
effect values Effect Range Low (ERL) and Effect Range Median
(ERM) classifications. The results indicate that the average values of
chromium, arsenic, copper, zinc, and lead in surface sediments all
reveal low pollution levels and are below ERL and ERM values. The
highest concentrations of trace elements were found close to
discharge points and in the inner bay, and were linked with high
percentages of clay minerals, pyrite and organic matter, which can
play a significant role in trapping and accumulating these elements.
The lowest concentrations of trace elements were found to be on the
shoreline of the bay, which contained high percentages of sand
fractions. It is postulated that the fine particles and trace elements are
disturbed by currents and tides, then transported and deposited in
deeper areas. The current track velocities recorded in Gymea Bay had
the capability to transport fine particles and trace element pollution
within the bay. As a result, hydrodynamic measurements were able to
provide useful information and to help explain the distribution of
sedimentary particles and geochemical properties. This may lead to
knowledge transfer to other bay systems, including those in remote
areas. These activities can be conducted at a low cost, and are
therefore also transferrable to developing countries. The advent of
portable instruments to measure trace elements in the field has also
contributed to the development of these lower cost and easily applied
methodologies available for use in remote locations and low-cost
economies.
Abstract: Biochar can be produced from the waste matter and its
application has been associated with returning of carbon in large
amounts into the soil. The impacts of this material on physical and
chemical properties of soil have been described. The biggest part of
the research work is dedicated to the hypothesis of this material’s
toxic effects on the soil life regarding its effect on the soil biological
component. At present, it has been worked on methods which could
eliminate these undesirable properties of biochar. One of the
possibilities is to mix biochar with organic material, such as compost,
or focusing on the natural processes acceleration in the soil. In the
experiment has been used as the addition of compost as well as the
elimination of toxic substances by promoting microbial activity in
aerated water environment. Biochar was aerated for 7 days in a
container with a volume of 20 l. This way modified biochar had six
times higher biomass production and reduce mineral nitrogen
leaching. Better results have been achieved by mixing biochar with
compost.
Abstract: This research work presents the surface
thermodynamics approach to M-TB/HIV-Human sputum
interactions. This involved the use of the Hamaker coefficient
concept as a surface energetics tool in determining the interaction
processes, with the surface interfacial energies explained using van
der Waals concept of particle interactions. The Lifshitz derivation for
van der Waals forces was applied as an alternative to the contact
angle approach which has been widely used in other biological
systems. The methodology involved taking sputum samples from
twenty infected persons and from twenty uninfected persons for
absorbance measurement using a digital Ultraviolet visible
Spectrophotometer. The variables required for the computations with
the Lifshitz formula were derived from the absorbance data. The
Matlab software tools were used in the mathematical analysis of the
data produced from the experiments (absorbance values). The
Hamaker constants and the combined Hamaker coefficients were
obtained using the values of the dielectric constant together with the
Lifshitz Equation. The absolute combined Hamaker coefficients
A132abs and A131abs on both infected and uninfected sputum samples
gave the values of A132abs = 0.21631x10-21Joule for M-TB infected
sputum and Ã132abs = 0.18825x10-21Joule for M-TB/HIV infected
sputum. The significance of this result is the positive value of the
absolute combined Hamaker coefficient which suggests the existence
of net positive van der waals forces demonstrating an attraction
between the bacteria and the macrophage. This however, implies that
infection can occur. It was also shown that in the presence of HIV,
the interaction energy is reduced by 13% conforming adverse effects
observed in HIV patients suffering from tuberculosis.
Abstract: Strong anthropogenic impact has uncontrolled
consequences on the nature of the soil. Hence, up-to-date sustainable
methods of soil state improvement are essential. Investigators provide
the evidence that biochar can positively effects physical, chemical,
and biological soil properties and the abundance of mycorrhizal fungi
which are in the focus of this study. The main aim of the present
investigation is to demonstrate the effect of two types of plant growth
promoting bacteria (PGPB) inoculums along with the beech wood
biochar and mineral N additives on mycorrhizal colonization.
Experiment has been set up in laboratory conditions with containers
filled with arable soil from the protection zone of the main water
source “Brezova nad Svitavou”. Lactuca sativa (lettuce) has been
selected as a model plant. Based on the obtained data, it can be
concluded that mycorrhizal colonization increased as the result of
combined influence of biochar and PGPB inoculums amendment. In
addition, correlation analyses showed that the numbers of main
groups of cultivated bacteria were dependent on the degree of
mycorrhizal colonization.
Abstract: Background: Breast milk may impact early brain
development, with potentially important biological, medical and
social implications. There is an important discussion on which is the
adequate breastfeeding extension to the development consolidation
and how the children breastfeeding affects their psychomotor
development, in the first year of life, and in following periods as
well. Some special fats (LC PUFA) contained in breast milk play a
key role in the brain’s maturation and cognitive development or
social skills. These capacities created during breastfeeding time
would be unfolded throughout all lifespan. Aim of the study: In our research, we have studied the effect of
breastfeeding in preschooler's psychomotor assessment.
Method: This study was conducted in a sample of 158 preschool
children in Vlorë, Albania. We have measured the psychometric
parameters of preschoolers with ASQ-3 (Age&Stage Questionnaires-
3). The studied sample was divided in three groups according to their
breastfeeding duration (3, 6 and 12 months). Results: Children breastfed for only 3 months have definitely
lower psychometric scores compared to the ones with 6 or more
months of breastfeeding (respectively 217 to 239 ASQ-3 scores). Six
and twelvemonth breastfed children have progressively more odds to
have high levels of psychomotor development comparing to those
with only 3 months of breastfeeding. The most affected psychometric
domains by shortness of breastfeeding are Communication and
Global motor. Conclusion: This leads to conclusion that to ensure high
psychomotor parameters during childhood is necessary breastfeeding
for at least 6 months.
Abstract: Cancer is still one of the serious diseases threatening
the lives of human beings. How to have an early diagnosis and
effective treatment for tumors is a very important issue. The animal
carcinoma model can provide a simulation tool for the studies of
pathogenesis, biological characteristics, and therapeutic effects.
Recently, drug delivery systems have been rapidly developed to
effectively improve the therapeutic effects. Liposome plays an
increasingly important role in clinical diagnosis and therapy for
delivering a pharmaceutic or contrast agent to the targeted sites.
Liposome can be absorbed and excreted by the human body, and is
well known that no harm to the human body. This study aimed to
compare the therapeutic effects between encapsulated (doxorubicin
liposomal, Lipodox) and un-encapsulated (doxorubicin, Dox)
anti-tumor drugs using magnetic resonance imaging (MRI).
Twenty-four New Zealand rabbits implanted with VX2 carcinoma at
left thighs were classified into three groups: control group (untreated),
Dox-treated group, and LipoDox-treated group, 8 rabbits for each
group. MRI scans were performed three days after tumor implantation.
A 1.5T GE Signa HDxt whole body MRI scanner with a high
resolution knee coil was used in this study. After a 3-plane localizer
scan was performed, three-dimensional (3D) fast spin echo (FSE)
T2-weighted Images (T2WI) was used for tumor volumetric
quantification. Afterwards, two-dimensional (2D) spoiled gradient
recalled echo (SPGR) dynamic contrast-enhanced (DCE) MRI was
used for tumor perfusion evaluation. DCE-MRI was designed to
acquire four baseline images, followed by contrast agent Gd-DOTA
injection through the ear vein of rabbit. A series of 32 images were
acquired to observe the signals change over time in the tumor and
muscle. The MRI scanning was scheduled on a weekly basis for a
period of four weeks to observe the tumor progression longitudinally.
The Dox and LipoDox treatments were prescribed 3 times in the first
week immediately after the first MRI scan; i.e. 3 days after VX2 tumor
implantation. ImageJ was used to quantitate tumor volume and time
course signal enhancement on DCE images. The changes of tumor size
showed that the growth of VX2 tumors was effectively inhibited for
both LipoDox-treated and Dox-treated groups. Furthermore, the tumor
volume of LipoDox-treated group was significantly lower than that of
Dox-treated group, which implies that LipoDox has better therapeutic effect than Dox. The signal intensity of LipoDox-treated group is
significantly lower than that of the other two groups, which implies
that targeted therapeutic drug remained in the tumor tissue. This study
provides a radiation-free and non-invasive MRI method for
therapeutic monitoring of targeted liposome on an animal tumor
model.