Abstract: Some Metapenaeus monoceros cox1 gene fragments were isolated, purified, sequenced, and comparatively analyzed with some other Crustacean Cox1 gene sequences (obtained from National Center for Biotechnology Information). This work was designed for testing the efficiency of this system in reconstruction of phylogenetic relations among some Crustacean species belonging to four genera (Metapenaeus, Artemia, Daphnia and Calanus). The single nucleotide polymorphism and haplotype diversity were calculated for all estimated mt-DNA fragments. The genetic distance values were 0.292, 0.015, 0.151, and 0.09 within Metapenaeus species, Calanus species, Artemia species, and Daphnia species, respectively. The reconstructed phylogenetic tree is clustered into some unique clades. Cytochrome oxidase subunit 1 gene (cox1) was a powerful system in reconstruction of phylogenetic relations among evaluated crustacean species.
Abstract: Nowadays, biometrical characterizations of Artemia
cysts are used as one of the most important factors in the study of
Artemia populations and intraspecific particularity; meanwhile these
characters can be used as economical indices. For example, typically
high hatching efficiency is possible due to the small diameter of
cysts (high number per gram); therefore small diameter of cysts
show someway high quality of cysts. This study was performed
during a ten year period, including two different ecological
conditions: rainy and drought. It is important from two different
aspects because it covers alteration of A. urmiana during ten years
also its variation in the best and worst environmental situations in
which salinity increased from 173.8 ppt in 1994 to 280.8 ppt in
2003/4. In this study the biometrical raw data of Artemia urmiana
cysts at seven stations from the Urmia Lake in 1994 and their seven
identical locations at 26 studied stations in 2003/4 were reanalyzed
again and compared together. Biometrical comparison of untreated
and decapsulated cysts in each of the seven similar stations showed a
highly significant variation between 1994 and 2003/4. Based on this
study, in whole stations the untreated and decapsulated cysts from
1994 were larger than cysts of 2003/4 without any exception. But
there was no logical relationship between salinity and chorion
thickness in the Urmia Lake. With regard to PCA analyses the
stations of two different studied years certainly have been separated
with factor 1 from each other. In conclusion, the interaction between
genetic and environmental factors can determine and explain
variation in the range of cysts diameter in Artemia.
Abstract: The aim of the study was to investigate phytochemical
properties, antimicrobial activity and cytotoxicity of Aloe vera. The
phytochemical screening of the extracts of leaves of A. vera revealed
the presence of bioactive compounds such as alkaloids, tannins,
flavonoids phenolic compounds, and etc. with absence of cyanogenic
glycosides. Three different solvents such as methanol, ethanol and
Di-Methyl sulfoxide were used to screen the antimicrobial activity of
A. vera leaves against four human clinical pathogens by agar well
diffusion method. The maximum antibacterial activities were
observed in methanol extract followed by ethanol and Di-Methyl
sulfoxide. It was also found that remarkable antibacterial activities
with methanolic and ethanolic extracts of A. vera compared with the
standard antibiotic, tetracycline that was not active against E. coli
and S. boydii and supported the view that A. vera is a potent
antimicrobial agent compared with the conventional antibiotic.
Moreover, the brine shrimps (Artemia salina) toxicity test exhibited
LC50 value was 569.52 ppm. The resulting data indicated that the A.
vera plant have less toxic effects on brine shrimp. Hence, it is
signified that Aloe vera plant extract is safe to be used as an
antimicrobial agent.
Abstract: Endemic Artemia franciscana populations can be found throughout the American continent and also as an introduced specie in several country all over the world, such as in the Mediterranean region where Artemia franciscana was identified as an invasive specie replacing native Artemia parthenogenetica and Artemia salina. In the present study, the characterization of the new invasive Artemia franciscana reported from Sabkhet Halk El-Menzel (Tunisia) was done based on the cysts biometry, nauplii instar-I length, Adult sexual dimorphism and fatty acid profile. The mean value of the diameter of non-decapsulated and decapsulated cysts, chorion thickness and naupliar length is 235.8, 226.3, 4.75 and 426.8 μm, respectively. Sexual dimorphism for adults specimen showed that maximal distance between compound eyes, diameter for compound eyes, length of first antenna and the abdomen length compared to the total body length ratio, are the most important variables for males and females discrimination with a total contribution of 62.39 %. The analysis of fatty acid methyl esters profile of decapsulated cysts resulted in low levels of linolenic acid (LLA, C18:3n-3) and high levels of eicosapentaenoic acid (EPA, C20:5n-3) with 3.11 and 11.10 %, respectively. Low quantity of docosahexaenoic acid (DHA, 22:6n-3) was also observed with 0.17 mg.g-1 dry weight.
Abstract: Artemia is one of the most conspicuous invertebrates
associated with aquaculture. It can be considered as a model
organism, offering numerous advantages for comprehensive and
multidisciplinary studies using morphologic or molecular methods.
Since DNA extraction is an important step of any molecular
experiment, a new and a rapid method of DNA extraction from adult
Artemia was described in this study. Besides, the efficiency of this
technique was compared with two widely used alternative techniques,
namely Chelex® 100 resin and SDS-chloroform methods. Data
analysis revealed that the new method is the easiest and the most cost
effective method among the other methods which allows a quick and
efficient extraction of DNA from the adult animal.
Abstract: Larval rearing and seed production of most of tetra fishes (Family: Characidae) is critical due to their small size larvae and limited numbers of spawning attempts. During the present study the effect of different live foods on growth and survival of neon tetra, Paracheirodon innesi larvae (length 3.1 ± 0.012mm, weight 0.048 ± 0.00015mg) and early fry (length = 6.44 ± 0.025mm, weight = 0.64 ± 0.003mg and 13 days old) was determined in two experiments. Experiment I was conducted for rearing the larvae by using mixed green water and Infusoria whereas, in Experiment II, early fry were fed with mixed zooplankton, decapsulated Artemia cyst and Artemia nauplii. The larvae fed on mixed green water showed significant (p