Abstract: Since the marine environmental conditions are
extremely different from the other ones, marine actinomycetes might
produce novel bioactive compounds. Therefore, actinomycete strains
were screened from marine water and sediment samples collected
from the coastal areas of Northern Vietnam. Ninety-nine
actinomycete strains were obtained on starch-casein agar media by
dilution technique, only seven strains, named HP112, HP12, HP411,
HPN11, HP 11, HPT13 and HPX12, showed significant antibacterial
activity against both gram-positive and gram-negative bacteria
(Bacillus subtilis ATCC 6633, Staphylococcus epidemidis ATCC
12228, Escherichia coli ATCC 11105). Further studies were carried
out with the most active HP411 strain against Candida albicans
ATCC 10231. This strain could grow rapidly on starch casein agar
and other media with high salt containing 7-10% NaCl at 28-30oC.
Spore-chain of HP411 showed an elongated and circular shape with
10 to 30 spores/chain. Identification of the strain was carried out by
employing the taxonomical studies including the 16S rRNA
sequence. Based on phylogenetic and phenotypic evidence it is
proposed that HP411 to be belongs to species Streptomyces
variabilis. The potent of the crude extract of fermentation broth of
HP411 that are effective against wide range of pathogens: both grampositive,
gram-negative and fungi. Further studies revealed that the
crude extract HP411 could obtain the anticancer activity for cancer
cell lines: Hep-G2 (liver cancer cell line); RD (cardiac and skeletal
muscle letters cell line); FL (membrane of the uterus cancer cell line).
However, the actinomycetes from marine ecosystem will be useful
for the discovery of new drugs in the future.
Abstract: Using ETABS software, this study analyzed 23
buildings to evaluate effects of mistakes during construction phase on
buildings structural behavior. For modelling, two different loadings
were assumed: 1) design loading and 2) loading due to the effects of
mistakes in construction phase. Research results determined that
considering traditional construction methods for buildings resulted in
a significant increase in dead loads and consequently intensified the
displacements and base-shears of buildings under seismic loads.
Abstract: Food contamination occurs during post process
handling. This leads to spoilage and growth of pathogenic
microorganisms in the food, thereby reducing its shelf life or
spreading of food borne diseases. Several methods are tried and one
of which is use of antimicrobial packaging. Here, papain, a protease
enzyme, is covalently immobilized with the help of glutarldehyde on
polyurethane and used as a food wrap to protect food from microbial
contamination. Covalent immobilization of papain was achieved at a
pH of 7.4; temperature of 4°C; glutaraldehyde concentration of 0.5%;
incubation time of 24h; and 50mg of papain. The formation of -C=Nobserved
in the Fourier transform infrared spectrum confirmed the
immobilization of the enzyme on the polymer. Immobilized enzyme
retained higher activity than the native free enzyme. The modified
polyurethane showed better reduction of Staphylococcus aureus
biofilm than bare polymer film (eight folds reduction in live colonies,
two times reduction in protein and 6 times reduction in
carbohydrates). The efficacy of this was studied by wrapping it over
S. aureus contaminated cottage cheese (paneer) and cheese and
stored at a temperature of 4°C for 7days. The modified film reduced
the bacterial contamination by eight folds when compared to the bare
film. FTIR also indicated reduction in lipids, sugars and proteins in
the biofilm.
Abstract: Preparation of nanoparticles of cerium oxide and
adsorption of bovine serum albumin on them were studied. Particle
size distribution and influence of pH on zeta potential of prepared
CeO2 were determined. Average size of prepared cerium oxide
nanoparticles was 9 nm. The simultaneous measurements of the
bovine serum albumin adsorption and zeta potential determination of
the (adsorption) suspensions were carried out. The adsorption
isotherms were found to be of typical Langmuir type; values of the
bovine serum albumin adsorption capacities were calculated.
Increasing of pH led to decrease of zeta potential and decrease of
adsorption capacity of cerium oxide nanoparticles. The maximum
adsorption capacity was found for strongly acid suspension (am =
118 mg/g). The samples of nanoceria with positive zeta potential
adsorbed more bovine serum albumin on the other hand, the samples
with negative zeta potential showed little or no protein adsorption.
Surface charge or better say zeta potential of CeO2 nanoparticles
plays the key role in adsorption of proteins on such type of materials.
Abstract: Through this paper we present a method for automatic
generation of ontological model from any data source using Model
Driven Architecture (MDA), this generation is dedicated to the
cooperation of the knowledge engineering and software engineering.
Indeed, reverse engineering of a data source generates a software
model (schema of data) that will undergo transformations to generate
the ontological model. This method uses the meta-models to validate
software and ontological models.
Abstract: The aim of this study is to determine the effect of age
and physiological status on progesterone and energy metabolism of
Ouled Djellal (O.D) breed ewes. 40 healthy ewes were divided into
two groups, primiparous and multiparous, with 20 ewes in each
group. The body weights (BW) (Kg) were 46.6 ± 4.20 and 59.2 ±
3.02, and consuming less 25 to 30% of their basal energetic
requirements. The values of serum glucose, triglycerides and
cholesterol were lower in pregnant than in non-pregnant ewes. The
high to very high significant differences were found during the 15th
week of pregnancy for glycaemia and triglyceridemia respectively.
Concerning serum progesterone, a very highly significant difference
(p
Abstract: The postharvest quality management of tomatoes is
important to limit the amount of losses that occur due to deterioration
between harvest and consumption. This study was undertaken to
investigate the effects of pre- and postharvest integrated agrotechnologies,
involving greenhouse microclimate and postharvest
storage conditions, on the postharvest quality attributes of four
tomato cultivars. Tomato fruit firmness, colour (hue angle (h°) and
L* value), pH and total soluble solids for the cultivars Bona,
Star 9037, Star 9009 and Zeal, grown in a fan-pad evaporativelycooled
and an open-ended naturally-ventilated tunnel, were harvested
at the mature-green stage. The tomatoes were stored for 28 days
under cold storage conditions, with a temperature of 13°C and RH of
85%, and under ambient air conditions, with a temperature of 23±
2°C and RH of 52± 4%. This study has provided information on the
effect of integrated pre-harvest and postharvest agro-technologies,
involving greenhouse microclimate and postharvest storage
environment on the postharvest quality attributes of four of the
tomato cultivars in South Africa. NVT-grown tomatoes retained
better textural qualities, but ripened faster by changing from green to
red faster, although these were reduced under cold storage conditions.
FPVT-grown tomatoes had lower firmness, but ripened slowly with
higher colour attributes. With cold storage conditions, the firmness of
FPVT-grown tomatoes was maintained. Cultivar Bona firmness and
colour qualities depreciated the fastest, but it had higher TSS content
and lower pH values. Star 9009 and Star 9037 presented better
quality, by retaining higher firmness and ripening slowly, but they
had the lowest TSS contents and high pH values, especially
Star 9037. Cold storage improved the firmness of tomato cultivars
with poor textural quality and faster colour changes.
Abstract: In today’s world, the LED display has been used for
presenting visual information under various circumstances. Such
information is an important intermediary in the human information
processing. Researchers have been investigated diverse factors that
influence this process effectiveness. The letter size is undoubtedly
one major factor that has been tested and recommended by many
standards and guidelines. However, viewing information on the
display from direct perpendicular position is a typical assumption
whereas many actual events are required viewing from the angles.
This current research aims to study the effect of oblique viewing
angle and viewing distance on ability to recognize alphabet, number,
and English word. The total of ten participants was volunteered to our
3 x 4 x 4 within subject study. Independent variables include three
distance levels (2, 6, and 12 m), four oblique angles (0, 45, 60, 75
degree), and four target types (alphabet, number, short word, and
long word). Following the method of constant stimuli our study
suggests that the larger oblique angle, ranging from 0 to 75 degree
from the line of sight, results in significant higher legibility threshold
or larger font size required (p-value < 0.05). Viewing distance factor
also shows to have significant effect on the threshold (p-value
Abstract: GRF, Growth regulating factor, genes encode a novel
class of plant-specific transcription factors. The GRF proteins play a
role in the regulation of cell numbers in young and growing tissues
and may act as transcription activations in growth and development
of plants. Identification of GRF genes and their expression are
important in plants to performance of the growth and development of
various organs. In this study, to better understanding the structural
and functional differences of GRFs family, 45 GRF proteins
sequences in A. thaliana, Z. mays, O. sativa, B. napus, B. rapa, H.
vulgare and S. bicolor, have been collected and analyzed through
bioinformatics data mining. As a result, in secondary structure of
GRFs, the number of alpha helices was more than beta sheets and in
all of them QLQ domains were completely in the biggest alpha helix.
In all GRFs, QLQ and WRC domains were completely protected
except in AtGRF9. These proteins have no trans-membrane domain
and due to have nuclear localization signals act in nuclear and they
are component of unstable proteins in the test tube.
Abstract: Aluminium and its alloys have excellent corrosion
resistant properties, ease of fabrication and high specific strength to
weight ratio. In this investigation an attempt has been made to study
the effect of different post weld heat treatment methods on the
mechanical and metallurgical properties of TIG welded joints of the
commercial aluminium alloy. Three different methods of post weld
heat treatments are solution heat treatment, artificial ageing and
combination of solution heat treatment and artificial aging are given
to TIG welded aluminium joints. Mechanical and metallurgical
properties of As welded joints of the aluminium alloys and post weld
heat treated joints of the aluminium alloys were examined.
Abstract: Esterification of p-bromo-m-cresol led to formation of
2-(4-bromo-3-methylphenoxy)acetate (1). 2-(4-Bromo-3-methyl
phenoxy)acetohydrazide (2) is derived from Compound (1) by
hydrazination. Compound (2) was reacted with different aromatic
aldehydes to yield N-(substituted benzylidiene)-2-(4-bromo-3-methyl
phenoxy)acetamide(3a-c). Cyclization of compound (3a-c) with
thioglycolic acid yielded 2-(4-bromo-3-methylphenoxy)-N-(4-oxo-2-
arylthiazolidin-3-yl) acetamide (4a-c). The newly synthesized
compounds were characterized on the basis of spectral studies and
evaluated for antibacterial and antifungal activities.
Abstract: Recently, there have been a lot of earthquakes in Japan.
It is necessary to promote seismic isolation devices for buildings. The
devices have been hardly diffused in attached houses, because the
devices are very expensive. We should develop a low-cost seismic
isolation device for detached houses. We suggested a new seismic
isolation device which uses a two-layer circular tube as a unit. If
hysteresis is produced in the two-layer circular tube under lateral
compression load, we think that the two-layer circular tube can have
energy absorbing capacity. It is necessary to contact the outer layer
and the inner layer to produce hysteresis. We have previously reported
how the inner layer comes in contact with the outer layer from a
perspective of analysis used mechanics of materials. We have clarified
that the inner layer comes in contact with the outer layer under a lateral
compression load. In this paper, we explored contact area between the
outer layer and the inner layer under a lateral compression load by
using FEA. We think that changing the inner layer’s thickness is
effective in increase the contact area. In order to change the inner
layer’s thickness, we changed the shape of the inner layer. As a result,
the contact area changes depending on the inner layer’s thickness.
Additionally, we experimented to check whether hysteresis occurs in
fact. As a consequence, we can reveal hysteresis in the two-layer
circular tube under the condition.
Abstract: Kigelia africana (Lam.) Benth. (Bignoniaceae) is a
reputed traditional remedy for various human ailments such as skin
diseases, microbial infections, melanoma, stomach troubles,
metabolic disorders, malaria and general pains. In spite of the fruit
being widely used for purposes related to its antibacterial and
antifungal properties, the chemical constituents associated with the
activity have not been fully identified. To elucidate the active
principles, we evaluated the antimicrobial activity of fruit extracts
and purified fractions against Staphylococcus aureus, Enterococcus
faecalis, Moraxella catarrhalis, Escherichia coli, Candida albicans
and Candida tropicalis. Shade-dried fruits were powdered and
extracted with hydroalcoholic (1:1) mixture by soaking at room
temperature for 72 h. The crude extract was further fractionated by
column chromatography, with successive elution using hexane,
dichloromethane, ethyl acetate, acetone and methanol. The
dichloromethane and ethyl acetate fractions were combined and
subjected to column chromatography to furnish a wax and oil from
the eluates of 20% and 40% ethyl acetate in hexane, respectively. The
GC-MS and GC×GC-MS results revealed that linoleic acid, linolenic
acid, palmitic acid, arachidic acid and stearic acid were the major
constituents in both oil and wax. The crude hydroalcoholic extract
exhibited the strongest activity with MICs of 0.125-0.5 mg/mL,
followed by the ethyl acetate (MICs = 0.125-1.0 mg/mL),
dichloromethane (MICs = 0.250-2.0 mg/mL), hexane (MICs = 0.25-
2.0 mg/mL), acetone (MICs = 0.5-2.0 mg/mL) and methanol (MICs =
1.0-2.0 mg/mL), whereas the wax (MICs = 2.0-4.0 mg/mL) and oil
(MICs = 4.0-8.0 mg/mL) showed poor activity. The study concludes
that synergistic interactions of chemical constituents could be
responsible for the antimicrobial activity of K. africana fruits, which
needs a more holistic approach to understand the mechanism of its
antimicrobial activity.
Abstract: A finite difference/front tracking method is used to
study the motion of three-dimensional deformable drops suspended in
plane Poiseuille flow at non-zero Reynolds numbers. A parallel
version of the code was used to study the behavior of suspension on a
reasonable grid resolution (grids). The viscosity and density of drops
are assumed to be equal to that of the suspending medium. The effect
of the Reynolds number is studied in detail. It is found that drops
with small deformation behave like rigid particles and migrate to an
equilibrium position about half way between the wall and the
centerline (the Segre-Silberberg effect). However, for highly
deformable drops there is a tendency for drops to migrate to the
middle of the channel, and the maximum concentration occurs at the
centerline. The effective viscosity of suspension and the fluctuation
energy of the flow across the channel increases with the Reynolds
number of the flow.
Abstract: Due to the advancement of Internet technology, online
learning is widely used in higher education institutions. Online
learning offers several means of communication, including online
forum. Through online forum, students and instructors are able to
discuss and share their knowledge and expertise without having a
need to attend the face-to-face, ordinary classroom session. The
purposes of this study are to analyze the students’ levels of
participation and critical thinking, types of action and factors
influencing their participation in online forum. A total of 41
postgraduate students undertaking a course in educational technology
from a public university in Malaysia were involved in this study. In
this course, the students participated in a weekly online forum as part
of the course requirement. Based on the log data file extracted from
the online forum, the students’ type of actions (view, add, update,
delete posts) and their levels of participation (passive, moderate or
active) were identified. In addition, the messages posted in the forum
were analyzed to gauge their level of critical thinking. Meanwhile,
the factors that might influence their online forum participation were
measured using a 24-items questionnaire. Based on the log data, a
total of 105 posts were sent by the participants. In addition, the
findings show that (i) majority of the students are moderate
participants, with an average of two to three posts per person, (ii)
viewing posts are the most frequent type of action (85.1%), and
followed by adding post (9.7%). Furthermore, based on the posts
they made, the most frequent type of critical thinking observed was
justification (50 input or 19.0%), followed by linking ideas and
interpretation (47 input or 18%), and novelty (38 input or 14.4%).
The findings indicate that online forum allows for social interaction
and can be used to measure the students’ critical thinking skills. In
order to achieve this, monitoring students’ activities in the online
forum is recommended.
Abstract: Off-site construction methods have played an
important role in the construction sector in the past few decades. It is
increasingly becoming a major alternative technique and strategic
direction compared to traditional in-situ method. It produces a
significant amount of value for the construction industry and the
economy more generally. To date, an impressive number of studies
have been lunched on the perceived perception of off-site
construction. However, it seems that a quantifying benefit on the
offsite construction area is lacking. Therefore, this paper examines
the recent research literature on the benefits of off- site construction
and provides future direction. In the beginning, this paper provides a
brief history and current value of the off-site construction followed
by a detailed discussion on the benefit of off-site construction. These
benefits include but not limited to time saving, quality improvement,
relieving skills shortages, cost reduction and productivity
improvement. Toward this end, off-site construction should learn
from other productive industry similar to services or manufacturing
industry by applying operational management tools and techniques
with extensive focus on employee empowerment will shed the light
on future uptake of Off-site construction. This study is of value in
providing scholars have a clear picture of perceived benefit of off-site
construction research and give an opportunities for future uptake of
off-site method.
Abstract: Cloud service brokering is a new service paradigm that
provides interoperability and portability of application across multiple
Cloud providers. In this paper, we designed Cloud service brokerage
system, anyBroker, supporting integrated service provisioning and
SLA based service lifecycle management. For the system design, we
introduce the system concept and whole architecture, details of main
components and use cases of primary operations in the system. These
features ease the Cloud service provider and customer’s concern and
support new Cloud service open market to increase Cloud service
profit and prompt Cloud service echo system in Cloud computing
related area.
Abstract: Fritillaria oranensis (Liliaceae) was described in 1874
by pomel from Algeria. Plant samples have been collected from the
mount of Tessala (Sidi-Bel-Abbes). The morphological features of
various organs of the plant are described in detail. In the
morphological part of the study, features of various organs of the
plants such as stem and leaf were determined and illustrated.
Ecological studies provide information about the physical and
chemical structure of soil types in Tessala Mountain. The aim of this
original investigation is to put forth ecological and anatomical
features of these species for the first time, but at the same time given
detailed account of the morphological characteristics of the stem and
leaf of Fritillaria oranensis.
Abstract: The purpose of this project is to carry out an analysis
and determine the profile of actual lean manufacturing processes in
the Metropolitan Area of Bucaramanga. Through the analysis of
qualitative and quantitative variables it was possible to establish how
these manufacturers develop production practices that ensure their
competitiveness and productivity in the market.
In this study, a random sample of metallurgic and wrought iron
companies was applied, following which a quantitative focus and
analysis was used to formulate a qualitative methodology for
measuring the level of lean manufacturing procedures in the industry.
A qualitative evaluation was also carried out through a multivariate
analysis using the Numerical Taxonomy System (NTSYS) program
which should allow for the determination of Lean Manufacturing
profiles.
Through the results it was possible to observe how the companies
in the sector are doing with respect to Lean Manufacturing Practices,
as well as identify the level of management that these companies
practice with respect to this topic. In addition, it was possible to
ascertain that there is no one dominant profile in the sector when it
comes to Lean Manufacturing.
It was established that the companies in the metallurgic and
wrought iron industry show low levels of Lean Manufacturing
implementation. Each one carries out diverse actions that are
insufficient to consolidate a sectoral strategy for developing a
competitive advantage which enables them to tie together a
production strategy.
Abstract: The aim of present study was to monitor the presence
of Trichodina sp. in Rainbow trout, Oncorhynchus mykiss collected
from various fish farms in the western provinces of Iran during
January, 2013- January, 2014. Out of 675 sampled fish 335, (49.16%)
were infested with Trichodina. The highest prevalence was observed
in the spring and winter followed by autumn and summer. In general,
the intensity of infection was low except in cases where outbreaks of
Trichodiniasis endangered the survival of fish in some ponds. In light
infestation Trichodina is usually present on gills, fins and skin of
apparently healthy fish. Clinical signs of Trichodiniasis only appear
on fish with heavy infections and cases of moderate ones that are
usually exposed to one or more stress factors including, rough
handling during transportation from ponds, overcrowdness,
malnutrition, high of free ammonia and low of oxygen concentration.
Clinical signs of Trichodiniasis in sampled fish were sluggish
movement, loss of appetite, black coloration, necrosis and ulcer on
different parts of the body, detached scales and excessive
accumulation of mucous in gill pouches. The most obvious
histopathological changes in diseased fish were sloughing of the
epidermal layer, aggregation of leucocytes and melanine-carrying
cells (between the dermis and hypodermis) and proliferative changes
including hyperplasia and hypertrophy of the epithelial lining cells of
gill filaments which resulted in fusion of secondary lamellae. Control
of Trichodiniasis, has been achieved by formalin bath treatment at a
concentration of 250 ppm for one hour.