Abstract: Yeast cells live in a constantly changing environment that requires the continuous adaptation of their genomic program in order to sustain their homeostasis, survive and proliferate. Due to the advancement of high throughput technologies, there is currently a large amount of data such as gene expression, gene deletion and protein-protein interactions for S. Cerevisiae under various environmental conditions. Mining these datasets requires efficient computational methods capable of integrating different types of data, identifying inter-relations between different components and inferring functional groups or 'modules' that shape intracellular processes. This study uses computational methods to delineate some of the mechanisms used by yeast cells to respond to environmental changes. The GRAM algorithm is first used to integrate gene expression data and ChIP-chip data in order to find modules of coexpressed and co-regulated genes as well as the transcription factors (TFs) that regulate these modules. Since transcription factors are themselves transcriptionally regulated, a three-layer regulatory cascade consisting of the TF-regulators, the TFs and the regulated modules is subsequently considered. This three-layer cascade is then modeled quantitatively using artificial neural networks (ANNs) where the input layer corresponds to the expression of the up-stream transcription factors (TF-regulators) and the output layer corresponds to the expression of genes within each module. This work shows that (a) the expression of at least 33 genes over time and for different stress conditions is well predicted by the expression of the top layer transcription factors, including cases in which the effect of up-stream regulators is shifted in time and (b) identifies at least 6 novel regulatory interactions that were not previously associated with stress-induced changes in gene expression. These findings suggest that the combination of gene expression and protein-DNA interaction data with artificial neural networks can successfully model biological pathways and capture quantitative dependencies between distant regulators and downstream genes.
Abstract: Cement, the most widely used construction material
is very brittle and characterized by low tensile strength and strain
capacity. Macro to nano fibers are added to cement to provide
tensile strength and ductility to it. Carbon Nanotube (CNT), one of
the nanofibers, has proven to be a promising reinforcing material in
the cement composites because of its outstanding mechanical
properties and its ability to close cracks at the nano level. The
experimental investigations for CNT reinforced cement is costly,
time consuming and involves huge number of trials. Mathematical
modeling of CNT reinforced cement can be done effectively and
efficiently to arrive at the mechanical properties and to reduce the
number of trials in the experiments. Hence, an attempt is made to
numerically study the effective mechanical properties of CNT
reinforced cement numerically using Representative Volume
Element (RVE) method. The enhancement in its mechanical
properties for different percentage of CNTs is studied in detail.
Abstract: Restoration research has become important on principle recently in Czech Republic. The reason is simple. More than 70 % of mined brown coal comes from the North Bohemian Basin these days. Open cast brown coal mining has lead to large damage on the landscape. Reclamation of phytotoxic areas is one of the serious problems in the North Bohemian Basin. It mainly concerns the areas with the occurrence of overburden rocks from the coal bed enriched with coal. The presented paper includes the characteristics of the important phytotoxic areas and the methodology of their reclamation. The results are documented with the long term monitoring of physical, mineralogical, chemical and pedological parameters of rocks in the testing areas.
Abstract: In the present work, study of the vibration of thin cylindrical shells made of a functionally gradient material (FGM) composed of stainless steel and nickel is presented. Material properties are graded in the thickness direction of the shell according to volume fraction power law distribution. The objective is to study the natural frequencies, the influence of constituent volume fractions and the effects of boundary conditions on the natural frequencies of the FG cylindrical shell. The study is carried out using third order shear deformation shell theory. The analysis is carried out using Hamilton's principle. The governing equations of motion of FG cylindrical shells are derived based on shear deformation theory. Results are presented on the frequency characteristics, influence of constituent volume fractions and the effects of clamped-free boundary conditions
Abstract: Many natural language expressions are ambiguous, and
need to draw on other sources of information to be interpreted.
Interpretation of the e word تعاون to be considered as a noun or a verb
depends on the presence of contextual cues. To interpret words we
need to be able to discriminate between different usages. This paper
proposes a hybrid of based- rules and a machine learning method for
tagging Arabic words. The particularity of Arabic word that may be
composed of stem, plus affixes and clitics, a small number of rules
dominate the performance (affixes include inflexional markers for
tense, gender and number/ clitics include some prepositions,
conjunctions and others). Tagging is closely related to the notion of
word class used in syntax. This method is based firstly on rules (that
considered the post-position, ending of a word, and patterns), and
then the anomaly are corrected by adopting a memory-based learning
method (MBL). The memory_based learning is an efficient method to
integrate various sources of information, and handling exceptional
data in natural language processing tasks. Secondly checking the
exceptional cases of rules and more information is made available to
the learner for treating those exceptional cases. To evaluate the
proposed method a number of experiments has been run, and in
order, to improve the importance of the various information in
learning.
Abstract: In mechanical and environmental engineering, mixed
convection is a frequently encountered thermal fluid phenomenon
which exists in atmospheric environment, urban canopy flows, ocean
currents, gas turbines, heat exchangers, and computer chip cooling
systems etc... . This paper deals with a numerical investigation of
mixed convection in a vertical heated channel. This flow results from
the mixing of the up-going fluid along walls of the channel with the
one issued from a flat nozzle located in its entry section. The fluiddynamic
and heat-transfer characteristics of vented vertical channels
are investigated for constant heat-flux boundary conditions, a
Rayleigh number equal to 2.57 1010, for two jet Reynolds number
Re=3 103 and 2104 and the aspect ratio in the 8-20 range. The system
of governing equations is solved with a finite volumes method and an
implicit scheme. The obtained results show that the turbulence and
the jet-wall interaction activate the heat transfer, as does the drive of
ambient air by the jet. For low Reynolds number Re=3 103, the
increase of the aspect Ratio enhances the heat transfer of about 3%,
however; for Re=2 104, the heat transfer enhancement is of about
12%. The numerical velocity, pressure and temperature fields are
post-processed to compute the quantities of engineering interest such
as the induced mass flow rate, and average Nusselt number, in terms
of Rayleigh, Reynolds numbers and dimensionless geometric
parameters are presented.
Abstract: This study explored the relationship between
occupational stress and the perceived effectiveness of men and
women managers in Ghanaian organizations. The exploration is
underlined by attempt to understand the degree to which male and
female managers in Ghanaian organizations experience occupational
stress at the workplace. The purpose is to examine the sources and
extents of occupational stress experienced by male and female
managers in Ghana. Data was collected using questionnaires and
analyzed using both descriptive statistics and correlation analysis.
The results showed that female managers in Ghana are more likely to
report of more stress experiences in the workplace than their male
counterparts. The female managers are more likely to perceive role
conflict and alienation as job stressors while the male managers
perceived blocked career as a major source of workplace stress. It is
concluded that despite the female managers experiencing enormous
level of occupational stress, there was no significant differences
between their managerial effectiveness and that of the male.
Abstract: There are many sources trough which the soil get
enriched and contaminated with REEs. The determination of REEs in
environmental samples has been limited because of the lack of
sensitive analytical techniques. Soil samples were collected from
four sites including open cast coal mine, natural coal burning, coal
washery and control in the coal field located in Dhanbad, India.
Total concentrations of rare earth elements (REEs) were determined
using the inductively coupled plasma atomic absorption spectrometry
in order to assess enrichment status in the coal field. Results showed
that the mean concentrations of La, Pr, Eu, Tb, Ho, and Tm in open
cast mine and natural coal burning sites were elevated compared to
the reference concentrations, while Ce, Nd, Sm, and Gd were
elevated in coal washery site. When compared to reference soil,
heavy REEs (HREEs) were enriched in open cast mines and natural
coal burning affected soils, however, the HREEs were depleted in the
coal washery sites. But, the Chondrite-normalization diagram showed
significant enrichment for light REEs (LREEs) in all the soils. High
concentration of Pr, Eu, Tb, Ho, Tm, and Lu in coal mining and coal
burning sites may pose human health risks. Factor analysis showed
that distribution and relative abundance of REEs of the coal washery
site is comparable with the control. Eventually washing or cleaning
of coal could significantly decrease the emission of REEs from coal
into the environment.
Abstract: The steady-state temperature for one-dimensional transpiration cooling system has been conducted experimentally and numerically to investigate the heat transfer characteristics of combined convection and radiation. The Nickel –Chrome (Ni-Cr) open-cellular porous material having porosity of 0.93 and pores per inch (PPI) of 21.5 was examined. The upper surface of porous plate was heated by the heat flux of incoming radiation varying from 7.7 - 16.6 kW/m2 whereas air injection velocity fed into the lower surface was varied from 0.36 - 1.27 m/s, and was then rearranged as Reynolds number (Re). For the report of the results in the present study, two efficiencies including of temperature and conversion efficiency were presented. Temperature efficiency indicating how close the mean temperature of a porous heat plate to that of inlet air, and increased rapidly with the air injection velocity (Re). It was then saturated and had a constant value at Re higher than 10. The conversion efficiency, which was regarded as the ability of porous material in transferring energy by convection after absorbed from heat radiation, decreased with increasing of the heat flux and air injection velocity. In addition, it was then asymptotic to a constant value at the Re higher than 10. The numerical predictions also agreed with experimental data very well.
Abstract: In this experimental investigation shake table tests
were conducted on two reduced models that represent normal single
room building constructed by Compressed Stabilized Earth Block
(CSEB) from locally available soil. One model was constructed with
earthquake resisting features (EQRF) having sill band, lintel band and
vertical bands to control the building vibration and another one was
without Earthquake Resisting Features. To examine the seismic
capacity of the models particularly when it is subjected to long-period
ground motion by large amplitude by many cycles of repeated
loading, the test specimen was shaken repeatedly until the failure.
The test results from Hi-end Data Acquisition system show that
model with EQRF behave better than without EQRF. This modified
masonry model with new material combined with new bands is used
to improve the behavior of masonry building.
Abstract: A well balanced numerical scheme based on
stationary waves for shallow water flows with arbitrary topography
has been introduced by Thanh et al. [18]. The scheme was
constructed so that it maintains equilibrium states and tests indicate
that it is stable and fast. Applying the well-balanced scheme for the
one-dimensional shallow water equations, we study the early shock
waves propagation towards the Phuket coast in Southern Thailand
during a hypothetical tsunami. The initial tsunami wave is generated
in the deep ocean with the strength that of Indonesian tsunami of
2004.
Abstract: The purpose of present paper was to investigate
perceptions of Korean secondary school students about social issues
related to biological sciences. Twenty issues were selected based on
topics of articles in the newspaper from 2005 to 2010. The issues were
categorized into biotechnology, health-disease and environment
domains. Subjects were 541 high school students (male 253 and
female 288). On the survey, students were asked to answer on 5-point
Lickert scales how they thought of the effect of biological phenomena
or events related to biological issues on the individual life and the
society. They perceived that the biological issues would be more
effectible on the society than on the individual life. Female students
had a little more perceptions than males.
Abstract: In recent years image watermarking has become an
important research area in data security, confidentiality and image
integrity. Many watermarking techniques were proposed for medical
images. However, medical images, unlike most of images, require
extreme care when embedding additional data within them because
the additional information must not affect the image quality and
readability. Also the medical records, electronic or not, are linked to
the medical secrecy, for that reason, the records must be confidential.
To fulfill those requirements, this paper presents a lossless
watermarking scheme for DICOM images. The proposed a fragile
scheme combines two reversible techniques based on difference
expansion for patient's data hiding and protecting the region of
interest (ROI) with tamper detection and recovery capability.
Patient's data are embedded into ROI, while recovery data are
embedded into region of non-interest (RONI). The experimental
results show that the original image can be exactly extracted from the
watermarked one in case of no tampering. In case of tampered ROI,
tampered area can be localized and recovered with a high quality
version of the original area.
Abstract: A given polynomial, possibly with multiple roots, is
factored into several lower-degree distinct-root polynomials with
natural-order-integer powers. All the roots, including multiplicities,
of the original polynomial may be obtained by solving these lowerdegree
distinct-root polynomials, instead of the original high-degree
multiple-root polynomial directly.
The approach requires polynomial Greatest Common Divisor
(GCD) computation. The very simple and effective process, “Monic
polynomial subtractions" converted trickily from “Longhand
polynomial divisions" of Euclidean algorithm is employed. It
requires only simple elementary arithmetic operations without any
advanced mathematics.
Amazingly, the derived routine gives the expected results for the
test polynomials of very high degree, such as p( x) =(x+1)1000.
Abstract: Bioleaching of spent catalyst using moderate thermophilic chemolithotrophic acidophiles in growth medium without Fe source was investigated with two different pulp densities and three different size fractions. All the experiments were conducted on shake flasks at a temperature of 65 °C. The leaching yield of Ni and Al was found to be promising with very high leaching yield of 92-96% followed by Al as 41-76%, which means both Ni and Al leaching were favored by the moderate thermophilic bioleaching compared to the mesophilic bioleaching. The acid consumption was comparatively higher for the 10% pulp density experiments. Comparatively minimal difference in the leaching yield with different size fractions and different pulp densities show no requirement of grinding and using low pulp density less than 10%. This process would rather be economical as well as eco-friendly process for future optimization of the recovery of metal values from spent catalyst.
Abstract: In this paper the use of sequential machines for recognizing actions taken by the objects detected by a general tracking algorithm is proposed. The system may deal with the uncertainty inherent in medium-level vision data. For this purpose, fuzzification of input data is performed. Besides, this transformation allows to manage data independently of the tracking application selected and enables adding characteristics of the analyzed scenario. The representation of actions by means of an automaton and the generation of the input symbols for finite automaton depending on the object and action compared are described. The output of the comparison process between an object and an action is a numerical value that represents the membership of the object to the action. This value is computed depending on how similar the object and the action are. The work concludes with the application of the proposed technique to identify the behavior of vehicles in road traffic scenes.
Abstract: Reinforced Concrete (RC) structures strengthened
with fiber reinforced polymer (FRP) lack in thermal resistance under
elevated temperatures in the event of fire. This phenomenon led to
the lining of strengthened concrete with thin high performance
cementitious composites (THPCC) to protect the substrate against
elevated temperature. Elevated temperature effects on THPCC, based
on different cementitious materials have been studied in the past but
high-alumina cement (HAC)-based THPCC have not been well
characterized. This research study will focus on the THPCC based on
HAC replaced by 60%, 70%, 80% and 85% of ground granulated
blast furnace slag (GGBS). Samples were evaluated by the
measurement of their mechanical strength (28 & 56 days of curing)
after exposed to 400°C, 600°C and 28°C of room temperature for
comparison and corroborated by their microstructure study. Results
showed that among all mixtures, the mix containing only HAC
showed the highest compressive strength after exposed to 600°C as
compared to other mixtures. However, the tensile strength of THPCC
made of HAC and 60% GGBS content was comparable to the
THPCC with HAC only after exposed to 600°C. Field emission
scanning electron microscopy (FESEM) images of THPCC
accompanying Energy Dispersive X-ray (EDX) microanalysis
revealed that the microstructure deteriorated considerably after
exposure to elevated temperatures which led to the decrease in
mechanical strength.
Abstract: As the web continues to grow exponentially, the idea
of crawling the entire web on a regular basis becomes less and less
feasible, so the need to include information on specific domain,
domain-specific search engines was proposed. As more information
becomes available on the World Wide Web, it becomes more difficult
to provide effective search tools for information access. Today,
people access web information through two main kinds of search
interfaces: Browsers (clicking and following hyperlinks) and Query
Engines (queries in the form of a set of keywords showing the topic
of interest) [2]. Better support is needed for expressing one's
information need and returning high quality search results by web
search tools. There appears to be a need for systems that do reasoning
under uncertainty and are flexible enough to recover from the
contradictions, inconsistencies, and irregularities that such reasoning
involves. In a multi-view problem, the features of the domain can be
partitioned into disjoint subsets (views) that are sufficient to learn the
target concept. Semi-supervised, multi-view algorithms, which
reduce the amount of labeled data required for learning, rely on the
assumptions that the views are compatible and uncorrelated. This
paper describes the use of semi-structured machine learning approach
with Active learning for the “Domain Specific Search Engines". A
domain-specific search engine is “An information access system that
allows access to all the information on the web that is relevant to a
particular domain. The proposed work shows that with the help of
this approach relevant data can be extracted with the minimum
queries fired by the user. It requires small number of labeled data and
pool of unlabelled data on which the learning algorithm is applied to
extract the required data.
Abstract: CTMA-bentonite and BTEA-Bentonite prepared by Na-bentonite cation exchanged with cetyltrimethylammonium(CTMA) and benzyltriethylammonium (BTEA). Products were characterized by XRD and IR techniques.The d001 spacing value of CTMA-bentonite and BTEA-bentonite are 7.54Å and 3.50Å larger than that of Na-bentonite at 100% cation exchange capacity, respectively. The IR spectrum showed that the intensities of OH stretching and bending vibrations of the two organoclays decreased greatly comparing to untreated Na-bentonite. Batch experiments were carried out at 303 K, 318 K and 333 K to obtain the sorption isotherms of Crystal violet onto the two organoclays. The results show that the sorption isothermal data could be well described by Freundlich model. The dynamical data for the two organoclays fit well with pseudo-second-order kinetic model. The adsorption capacity of CTMA-bentonite was found higher than that of BTEA-Bentonite. Thermodynamic parameters such as changes in the free energy (ΔG°), the enthalpy (ΔH°) and the entropy (ΔS°) were also evaluated. The overall adsorption process of Crystal violet onto the two organoclays were spontaneous, endothermic physisorption. The CTMA-bentonite and BTEA-Bentonite could be employed as low-cost alternatives to activated carbon in wastewater treatment for the removal of color which comes from textile dyes.
Abstract: Shadoo protein (Sho) was described in 2003 as the newest member of Prion protein superfamily [1]. Sho has similar structural motifs like prion protein (PrP) that is known for its central role in transmissible spongiform enchephalopathies. Although a great number of functions have been proposed, the exact physiological function of PrP is not known yet. Investigation of the function and localization of Sho may help us to understand the function of the Prion protein superfamily. Analyzing the subcellular localization of YFP-tagged forms of Sho, we detected the protein in the plasma membrane and in the nucleus of various cell lines. To reveal the localization of the endogenous protein we generated antibodies against Shadoo as well as employed commercially available anti-Shadoo antibodies: i) EG62 anti-mouse Shadoo antibody generated by Eurogentec Ltd.; ii) S-12 anti-human Shadoo antibody by Santa Cruz Biotechnology Inc.; iii) R-12 anti-mouse Shadoo antibody by Santa Cruz Biotechnology Inc.; iv) SPRN antibody against human Shadoo by Abgent Inc. We carried out immunocytochemistry on non-transfected HeLa, Zpl 2-1, Zw 3-5, GT1-1, GT1-7 and SHSY5Y cells as well as on YFP-Sho, Sho-YFP, and YFP-GPI transfected HeLa cells. Their specificity (in antibody-peptide competition assay) and co-localization (with the YFP signal) were assessed.