Abstract: The process parameters, temperature, pH and
substrate concentration, were optimized for the production of
gentamicin using Micromonouspora echinospora. Experiments were
carried out according to central composite design in response surface
method. The optimum conditions for the maximum production of
gentamicin were found to be: temperature – 31.7oC, pH – 6.8 and
substrate concentration – 3%. At these optimized conditions the
production of gentamicin was found to be – 1040 mg/L. The R2 value
of 0.9465 indicates a good fitness of the model.
Abstract: The nature, prevalence, cellular composition of
leukocyte infiltrates and immunohistochemical characteristics of
their constituent cells in the liver of patients with chronic viral
hepatitis B and C were investigated. It was found that the area of
distribution and cellular composition of infiltrates depended on the
virus type and process activity. The expediency of
immunohistochemical study using leukocyte infiltrates from liver
biopsies of patients with viral hepatitis aimed at clarifying diagnosis,
making prognosis, and choice of optimal treatment with elements of
immune correction is emphasized.
Abstract: 16-Mercaptohexadecanoic acid (MHDA) and tripeptide glutathione conjugated with gold nanoparticles (Au-NPs) are characterized by Fourier Transform InfaRared (FTIR) spectroscopy combined with Surface-enhanced Raman scattering (SERS) spectroscopy. Surface Plasmon Resonance (SPR) technique based on FTIR spectroscopy has become an important tool in biophysics, which is perspective for the study of organic compounds. FTIR-spectra of MHDA shows the line at 2500 cm-1 attributed to thiol group which is modified by presence of Au-NPs, suggesting the formation of bond between thiol group and gold. We also can observe the peaks originate from characteristic chemical group. A Raman spectrum of the same sample is also promising. Our preliminary experiments confirm that SERS-effect takes place for MHDA connected with Au-NPs and enable us to detected small number (less than 106 cm-2) of MHDA molecules. Combination of spectroscopy methods: FTIR and SERS – enable to study optical properties of Au- NPs and immobilized bio-molecules in context of a bio-nano-sensors.
Abstract: A study has been carried out to determine the effect of
coating two commercial glass-ionomer cements in either petroleum
jelly or wax. After coating, specimens were stored in water for 24 or
168 hours, then the coating removed and the surface examined.
Coating in wax was found to increase the surface hardness
significantly compared with the uncoated control, whereas coating
the specimens in petroleum jelly led to only a slight increase in
surface hardness. Coating in wax led to no detectable ion release
after either 24 or 168 hours, though there was some ion release after
the coating had been removed and the specimens exposed to water
for a further 24 hours. This shows that soluble species remained in
these specimens. Overall, this study confirms the idea that immature
glass-ionomers should be protected from early exposure to moisture,
and that the protection offered by petroleum jelly is only modest.
Abstract: In this paper we intend to ascertain the state of the art on multifingered end-effectors, also known as robotic hands or dexterous robot hands, and propose an experimental setup for an innovative task based design approach, involving cutting edge technologies in motion capture. After an initial description of the capabilities and complexity of a human hand when grasping objects, in order to point out the importance of replicating it, we analyze the mechanical and kinematical structure of some important works carried out all around the world in the last three decades and also review the actuators and sensing technologies used. Finally we describe a new design philosophy proposing an experimental setup for the first stage using recent developments in human body motion capture systems that might lead to lighter and always more dexterous robotic hands.
Abstract: Recently, the design is becoming important in product development. The technology which is a strong point of Japan is immediately caught up by the foreign countries, and the price competition begins. Therefore companies tend to plan differentiation of products by the design or a color. The purpose of my work was to consider the optimal color for using by product development. We needed to clarify the thing leading to color preference for this purpose. Two kinds of investigations were made. By the first investigation, we found out that a geographical factor difference existed in color preference. Then, investigation which regarded the difference as latitude was conducted. However, the result expected from the difference in latitude was not obtained. It seems that it is necessary to set up difference of latitude a little more greatly, or to reexamine by other geographical factors.
Abstract: A simultaneous study on indoor and outdoor particulate matter concentrations was done in five elementary schools in central parts of Tehran, Iran. Three sizes of particles including PM10, PM2.5 and PM1.0 were measured in 13 classrooms within this schools during winter (January, February and March) 2009. A laserbased portable aerosol spectrometer Model Grimm-1.108, was used for the continuous measurement of particles. The average indoor concentration of PM10, PM2.5 and PM1.0 in studied schools were 274 μg/m3, 42 μg/m3 and 19 μg/m3 respectively; and average outdoor concentrations of PM10, PM2.5 and PM1.0 were evaluated to be 22 μg/m3, 38 μg/m3 and 140 μg/m3 respectively.
Abstract: The iron loss is a source of detuning in vector controlled
induction motor drives if the classical rotor vector controller is used for
decoupling. In fact, the field orientation will not be satisfied and the
output torque will not truck the reference torque mostly used by Loss
Model Controllers (LMCs). In addition, this component of loss, among
others, may be excessive if the vector controlled induction motor is
driving light loads. In this paper, the series iron loss model is used to
develop a vector controller immune to iron loss effect and then an LMC
to minimize the total power loss using the torque generated by the speed
controller.
Abstract: In this paper, naturally immobilized lipase, Carica
papaya lipase, catalyzed biodiesel production from fish oil was
studied. The refined fish oil, extracted from the discarded parts of
fish, was used as a starting material for biodiesel production. The
effects of molar ratio of oil: methanol, lipase dosage, initial water
activity of lipase, temperature and solvent were investigated. It was
found that Carica papaya lipase was suitable for methanolysis of fish
oil to produce methyl ester. The maximum yield of methyl ester
could reach up to 83% with the optimal reaction conditions: oil:
methanol molar ratio of 1: 4, 20% (based on oil) of lipase, initial
water activity of lipase at 0.23 and 20% (based on oil) of tert-butanol
at 40oC after 18 h of reaction time. There was negligible loss in
lipase activity even after repeated use for 30 cycles.
Abstract: Droplet size distributions in the cold spray of a fuel
are important in observed combustion behavior. Specification of
droplet size and velocity distributions in the immediate downstream
of injectors is also essential as boundary conditions for advanced
computational fluid dynamics (CFD) and two-phase spray transport
calculations. This paper describes the development of a new model to
be incorporated into maximum entropy principle (MEP) formalism
for prediction of droplet size distribution in droplet formation region.
The MEP approach can predict the most likely droplet size and
velocity distributions under a set of constraints expressing the
available information related to the distribution.
In this article, by considering the mechanisms of turbulence
generation inside the nozzle and wave growth on jet surface, it is
attempted to provide a logical framework coupling the flow inside the
nozzle to the resulting atomization process. The purpose of this paper
is to describe the formulation of this new model and to incorporate it
into the maximum entropy principle (MEP) by coupling sub-models
together using source terms of momentum and energy. Comparison
between the model prediction and experimental data for a gas turbine
swirling nozzle and an annular spray indicate good agreement
between model and experiment.
Abstract: Simultaneous effects of temperature, immersion time, salt concentration, sucrose concentration, pressure and convective dryer temperature on the combined osmotic dehydration - convective drying of edible button mushrooms were investigated. Experiments were designed according to Central Composite Design with six factors each at five different levels. Response Surface Methodology (RSM) was used to determine the optimum processing conditions that yield maximum water loss and rehydration ratio and minimum solid gain and shrinkage in osmotic-convective drying of edible button mushrooms. Applying surfaces profiler and contour plots optimum operation conditions were found to be temperature of 39 °C, immersion time of 164 min, salt concentration of 14%, sucrose concentration of 53%, pressure of 600 mbar and drying temperature of 40 °C. At these optimum conditions, water loss, solid gain, rehydration ratio and shrinkage were found to be 63.38 (g/100 g initial sample), 3.17 (g/100 g initial sample), 2.26 and 7.15%, respectively.
Abstract: Nuclear matrix protein 22 (NMP22) is a FDA approved
biomarker for bladder cancer. The objective of this study is to develop
a simple NMP22 immumosensor (NMP22-IMS) for accurate
measurement of NMP22. The NMP22-IMS was constructed with
NMP22 antibody immobilized on screen-printed carbon electrodes.
The construction procedures and antibody immobilization are simple.
Results showed that the NMP22-IMS has an excellent (r2³0.95)
response range (20 – 100 ng/mL). In conclusion, a simple and reliable
NMP22-IMS was developed, capable of precisely determining urine
NMP22 level.
Abstract: In this paper we address the issue of classifying the fluorescent intensity of a sample in Indirect Immuno-Fluorescence (IIF). Since IIF is a subjective, semi-quantitative test in its very nature, we discuss a strategy to reliably label the image data set by using the diagnoses performed by different physicians. Then, we discuss image pre-processing, feature extraction and selection. Finally, we propose two ANN-based classifiers that can separate intrinsically dubious samples and whose error tolerance can be flexibly set. Measured performance shows error rates less than 1%, which candidates the method to be used in daily medical practice either to perform pre-selection of cases to be examined, or to act as a second reader.
Abstract: The ability of the brain to organize information and generate the functional structures we use to act, think and communicate, is a common and easily observable natural phenomenon. In object-oriented analysis, these structures are represented by objects. Objects have been extensively studied and documented, but the process that creates them is not understood. In this work, a new class of discrete, deterministic, dissipative, host-guest dynamical systems is introduced. The new systems have extraordinary self-organizing properties. They can host information representing other physical systems and generate the same functional structures as the brain does. A simple mathematical model is proposed. The new systems are easy to simulate by computer, and measurements needed to confirm the assumptions are abundant and readily available. Experimental results presented here confirm the findings. Applications are many, but among the most immediate are object-oriented engineering, image and voice recognition, search engines, and Neuroscience.
Abstract: As in today's semiconductor industries test costs can make up to 50 percent of the total production costs, an efficient test error detection becomes more and more important. In this paper, we present a new machine learning approach to test error detection that should provide a faster recognition of test system faults as well as an improved test error recall. The key idea is to learn a classifier ensemble, detecting typical test error patterns in wafer test results immediately after finishing these tests. Since test error detection has not yet been discussed in the machine learning community, we define central problem-relevant terms and provide an analysis of important domain properties. Finally, we present comparative studies reflecting the failure detection performance of three individual classifiers and three ensemble methods based upon them. As base classifiers we chose a decision tree learner, a support vector machine and a Bayesian network, while the compared ensemble methods were simple and weighted majority vote as well as stacking. For the evaluation, we used cross validation and a specially designed practical simulation. By implementing our approach in a semiconductor test department for the observation of two products, we proofed its practical applicability.
Abstract: This study presents a mathematical modeling approach to the planning of HIV therapies on an individual basis. The model replicates clinical data from typical-progressors to AIDS for all stages of the disease with good agreement. Clinical data from rapid-progressors and long-term non-progressors is also matched by estimation of immune system parameters only. The ability of the model to reproduce these phenomena validates the formulation, a fact which is exploited in the investigation of effective therapies. The therapy investigation suggests that, unlike continuous therapy, structured treatment interruptions (STIs) are able to control the increase in both the drug-sensitive and drug-resistant virus population and, hence, prevent the ultimate progression from HIV to AIDS. The optimization results further suggest that even patients characterised by the same progression type can respond very differently to the same treatment and that the latter should be designed on a case-by-case basis. Such a methodology is presented here.
Abstract: The removal of hydrogen sulphide is required for reasons of health, odour problems, safety and corrosivity problems. The means of removing hydrogen sulphide mainly depend on its concentration and kind of medium to be purified. The paper deals with a method of hydrogen sulphide removal from the air by its catalytic oxidation to elemental sulphur with the use of Fe-EDTA complex. The possibility of obtaining fibrous filtering materials able to remove small concentrations of H2S from the air were described. The base of these materials is fibrous ion exchanger with Fe(III)- EDTA complex immobilized on their functional groups. The complex of trivalent iron converts hydrogen sulphide to elemental sulphur. Bivalent iron formed in the reaction is oxidized by the atmospheric oxygen, so complex of trivalent iron is continuously regenerated and the overall process can be accounted as pseudocatalytic. In the present paper properties of several fibrous catalysts based on ion exchangers with different chemical nature (weak acid,weak base and strong base) were described. It was shown that the main parameters affecting the process of catalytic oxidation are:concentration of hydrogen sulphide in the air, relative humidity of the purified air, the process time and the content of Fe-EDTA complex in the fibres. The data presented show that the filtering layers with anion exchange package are much more active in the catalytic processes of hydrogen sulphide removal than cation exchanger and inert materials. In the addition to the nature of the fibres relative air humidity is a critical factor determining efficiency of the material in the air purification from H2S. It was proved that the most promising carrier of the Fe-EDTA catalyst for hydrogen sulphide oxidation are Fiban A-6 and Fiban AK-22 fibres.
Abstract: In this study, aerobic digestion of tannery industry
wastewater was carried out using mixed culture obtained from
common effluent treatment plant treating tannery wastewater. The
effect of pH, temperature, inoculum concentration, agitation speed
and initial substrate concentration on the reduction of organic matters
were found. The optimum conditions for COD reduction was found
to be pH - 7 (60%), temperature - 30ÔùªC (61%), inoculum
concentration - 2% (61%), agitation speed - 150rpm (65%) and initial
substrate concentration - 1560 mg COD/L (74%). Kinetics studies
were carried by using Monod model, First order, Diffusional model
and Singh model. From the results it was found that the Monod
model suits well for the degradation of tannery wastewater using
mixed microbial consortium.
Abstract: Simultaneous effects of temperature, immersion time, salt concentration, sucrose concentration, pressure and convective dryer temperature on the combined osmotic dehydration - convective drying of edible button mushrooms were investigated. Experiments were designed according to Central Composite Design with six factors each at five different levels. Response Surface Methodology (RSM) was used to determine the optimum processing conditions that yield maximum water loss and rehydration ratio and minimum solid gain and shrinkage in osmotic-convective drying of edible button mushrooms. Applying surfaces profiler and contour plots optimum operation conditions were found to be temperature of 39 °C, immersion time of 164 min, salt concentration of 14%, sucrose concentration of 53%, pressure of 600 mbar and drying temperature of 40 °C. At these optimum conditions, water loss, solid gain, rehydration ratio and shrinkage were found to be 63.38 (g/100 g initial sample), 3.17 (g/100 g initial sample), 2.26 and 7.15%, respectively.
Abstract: Nowadays, butyl acetate, a pineapple flavor has been applied widely in food, beverage, cosmetic and pharmaceutical industries. In this study, Butyl acetate, a flavor ester was successfully synthesized via green synthesis of enzymatic reaction route. Commercial immobilized lipase from Rhizomucor miehei (Lipozyme RMIM) was used as biocatalyst in the esterification reaction between acetic acid and butanol. Various reaction parameters such as reaction time (RT), temperature (T) and amount of enzyme (E) were chosen to optimize the reaction synthesis in solvent-free system. The optimum condition to produce butyl acetate was at reaction time (RT), 18 hours; temperature (T), 37°C and amount of enzyme, 25 % (w/w of total substrate). Analysis of yield showed that at optimum condition, >78 % of butyl acetate was produced. The product was confirmed as butyl acetate from FTIR analysis whereby the presence of an ester group was observed at wavenumber of 1742 cm-1.