Abstract: In this study, six bacterial isolates of a slightly
thermophilic organism from the Sg. Klah hot spring, Malaysia were
successfully isolated and designated as M7T55D1, M7T55D2,
M7T55D3, M7T53D1, M7T53D2 and M7T53D3 respectively. The
bacterial isolates were screened for their cellulose hydrolytic ability
on Carboxymethlycellulose agar medium. The isolated bacterial
strains were identified morphologically, biochemically and
molecularly with the aid of 16S rDNA sequencing. All of the bacteria
showed their optimum growth at a slightly alkaline pH of 7.5 with a
temperature of 55°C. All strains were Gram-negative, non-spore
forming type, strictly aerobic, catalase-positive and oxidase-positive
with the ability to produce thermostable cellulase. Based on BLASTn
results, bacterial isolates of M7T55D2 and M7T53D1 gave the
highest homology (97%) with similarity to Tepidimonas ignava while
isolates M7T55D1, M7T55D3, M7T53D2 and M7T53D3 showed
their closest homology (97%-98%) with Tepidimonas thermarum.
These cellulolytic thermophiles might have a commercial potential to
produce valuable thermostable cellulase.
Abstract: Multipotent mesenchymal stromal cells (MSCs)
possess immunomodulatory properties. The effect of MSCs on the
crucial cellular immunity compartment – T-cells is of a special
interest. It is known that MSC tissue niche and expected milieu of
their interaction with T- cells are characterized by low oxygen
concentration, whereas the in vitro experiments usually are carried
out at a much higher ambient oxygen (20%). We firstly evaluated
immunomodulatory effects of MSCs on T-cells at tissue-related
oxygen (5%) after interaction implied cell-to-cell contacts and
paracrine factors only. It turned out that MSCs under reduced oxygen
can effectively suppress the activation and proliferation of PHAstimulated
T-cells and can provoke decrease in the production of
proinflammatory and increase in anti-inflammatory cytokines. In
hypoxia some effects were amplified (inhibition of proliferation, antiinflammatory
cytokine profile shift). This impact was more evident
after direct cell-to-cell interaction; lack of intercellular contacts could
revoke the potentiating effect of hypoxia.
Abstract: We used high-precision Global Positioning System
(GPS) to geodetically constrain the motion of stations in the
Darjiling-Sikkim Himalayan (DSH) wedge and examine the
deformation at the Indian-Tibetan plate boundary using IGS
(International GPS Service) fiducial stations. High-precision GPS
based displacement and velocity field was measured in the DSH
between 1997 and 2009. To obtain additional insight north of the
Indo-Tibetan border and in the Darjiling-Sikkim-Tibet (DaSiT)
wedge, published velocities from four stations J037, XIGA, J029 and
YADO were also included in the analysis. India-fixed velocities or
the back-slip was computed relative to the pole of rotation of the
Indian Plate (Latitude 52.97 ± 0.22º, Longitude - 0.30 ± 3.76º, and
Angular Velocity 0.500 ± 0.008º/ Myr) in the DaSiT wedge.
Dislocation modelling was carried out with the back-slip to model the
best possible solution of a finite rectangular dislocation or the
causative fault based on dislocation theory that produced the
observed back-slip using a forward modelling approach. To find the
best possible solution, three different models were attempted. First,
slip along a single thrust fault, then two thrust faults and in finally,
three thrust faults were modelled to simulate the back-slip in the
DaSiT wedge. The three-fault case bests the measured displacements
and is taken as the best possible solution.
Abstract: In order to evaluate the performance of a unified power
flow controller (UPFC), mathematical models for steady state and
dynamic analysis are to be developed. The steady state model is
mainly concerned with the incorporation of the UPFC in load flow
studies. Several load flow models for UPFC have been introduced
in literature, and one of the most reliable models is the decoupled
UPFC model. In spite of UPFC decoupled load flow model simplicity,
it is more robust compared to other UPFC load flow models and it
contains unique capabilities. Some shortcoming such as additional
set of nonlinear equations are to be solved separately after the load
flow solution is obtained. The aim of this study is to investigate the
different control strategies that can be realized in the decoupled load
flow model (individual control and combined control), and the impact
of the location of the UPFC in the network on its control parameters.
Abstract: Biological conversion of biomass to methane has
received increasing attention in recent years. Grasses have been
explored for their potential anaerobic digestion to methane. In this
review, extensive literature data have been tabulated and classified.
The influences of several parameters on the potential of these
feedstocks to produce methane are presented. Lignocellulosic
biomass represents a mostly unused source for biogas and ethanol
production. Many factors, including lignin content, crystallinity of
cellulose, and particle size, limit the digestibility of the hemicellulose
and cellulose present in the lignocellulosic biomass. Pretreatments
have used to improve the digestibility of the lignocellulosic biomass.
Each pretreatment has its own effects on cellulose, hemicellulose and
lignin, the three main components of lignocellulosic biomass. Solidstate
anaerobic digestion (SS-AD) generally occurs at solid
concentrations higher than 15%. In contrast, liquid anaerobic
digestion (AD) handles feedstocks with solid concentrations between
0.5% and 15%. Animal manure, sewage sludge, and food waste are
generally treated by liquid AD, while organic fractions of municipal
solid waste (OFMSW) and lignocellulosic biomass such as crop
residues and energy crops can be processed through SS-AD. An
increase in operating temperature can improve both the biogas yield
and the production efficiency, other practices such as using AD
digestate or leachate as an inoculant or decreasing the solid content
may increase biogas yield but have negative impact on production
efficiency. Focus is placed on substrate pretreatment in anaerobic
digestion (AD) as a means of increasing biogas yields using today’s
diversified substrate sources.
Abstract: Application of hulls processing technologies, based on high-concentrated energy sources (laser and plasma technologies), allow improve shipbuilding production. It is typical for high-speed vessels construction using steel and aluminum alloys with high precision hulls required. Report describes high-performance technologies for plasma welding (using direct current of reversed polarity), laser, and hybrid laser-arc welding of hulls structures developed by JSC “SSTC”
Abstract: This paper analyzes innovation trends in South Korea
by means of the number of patent applications filed by residents and
nonresidents during the period 1965 to 2012. Making use of patent
data released by the World Intellectual Property Organization
(WIPO), we search for the presence of multiple structural changes in
patent application series in this country. These changes may suggest
that firms’ innovative activity has been modified as a result of
implementing some science, technology and innovation (STI)
policies. Accordingly, the new regulations implemented in this
country in the last decades have influenced its innovative activity.
The question conducting this research is thus how STI policies in
South Korea have influenced its innovation activity. The results
confirm the existence of multiple structural changes in the series of
patent applications resulting from alternative STI policies
implemented during these years.
Abstract: The fight against climate change and the replacement
of fossil energies nearing exhaustion gradually emerge as major
societal and economic challenges. It is possible to develop common
dates of low commercial value, and put on the local and international
market a new generation of products with high added values such as
bio ethanol. Besides its use in chemical synthesis, bio ethanol can be
blended with gasoline to produce a clean fuel while improving the
octane.
Abstract: Fibrin degradation is an important part in prevention
or treatment of intravascular thrombosis and cardiovascular diseases.
Plasmin like fibrinolytic enzymes has given new hope to patient with
cardiovascular diseases by treating fibrin aggregation related diseases
with traditional plasminogen activator which have many side effects.
Various researches involving wide range of sources for production of
fibrinolytic proteases, from bacteria, fungi, insects and fermented
foods. But few have looked into endophytic fungi as a potential
source. Sixteen (16) endophytic fungi were isolated from Hibiscus sp.
leaves from six different locations in Shah Alam, Selangor. Only two
endophytic fungi, FH3 and S13 showed positive fibrinolytic protease
activities. FH3 produced 5.78cm and S13 produced 4.48cm on Skim
Milk Agar after 4 days of incubation at 27°C. Fibrinolytic activity
was observed; 3.87cm and 1.82cm diameter clear zone on fibrin plate
of FH3 and S13 respectively. 18srRNA was done for identification of
the isolated fungi with positive fibrinolytic protease. S13 had the
highest similarity (100%) to that of Penicillium citrinum strain TG2
and FH3 had the highest similarity (99%) to that of Fusarium sp.
FW2PhC1, Fusarium sp. 13002, Fusarium sp. 08006, Fusarium
equiseti strain Salicorn 8 and Fungal sp. FCASAn-2. Media
composition variation showed the effects of carbon nitrogen on
protein concentration, where the decrement of 50% of media
composition caused drastic decrease in protease of FH3 from 1.081 to
0.056 and also S13 from 2.946 to 0.198.
Abstract: The study examines the influence of marital status on
consumers of products and services using blogs as a source of
information. A pre-designed questionnaire was used to collect the
primary data from the respondents (experiences). Data were collected
from one hundred and eighty seven respondents residing in and
around the Emirates of Sharjah and Dubai of the United Arab
Emirates. The collected data was analyzed with the help of statistical
tools such as averages, percentages, factor analysis, Student’s t-test
and Structural Equation Modelling Technique.
Objectives of the study are to know the reasons how married and
unmarried or single consumers of products and services are
motivated to use blogs as a source of information, to know whether
the consumers of products and services irrespective of their marital
status share their views and experiences with other bloggers and to
know the respondents’ future intentions towards blogging.
The study revealed the following: Majority of the respondents
have the motivation to blog because they are willing to receive
comments on what they post about services, convenience of blogs to
search for information about services and products, by blogging
respondents share information on the symptoms of a disease/ disorder
that may be experienced by someone, helps to share information
about ready to cook mix products and are keen to spend more time
blogging in the future.
Abstract: A simple multi-wavelength passively Q-switched
Erbium-doped fiber laser (EDFL) is demonstrated using low cost
multi-walled carbon nanotubes (MWCNTs) based saturable absorber
(SA), which is prepared using polyvinyl alcohol (PVA) as a host
polymer. The multi-wavelength operation is achieved based on
nonlinear polarization rotation (NPR) effect by incorporating 50 m
long photonic crystal fiber (PCF) in the ring cavity. The EDFL
produces a stable multi-wavelength comb spectrum for more than 14
lines with a fixed spacing of 0.48 nm. The laser also demonstrates a
stable pulse train with the repetition rate increases from 14.9 kHz to
25.4 kHz as the pump power increases from the threshold power of
69.0 mW to the maximum pump power of 133.8 mW. The minimum
pulse width of 4.4 μs was obtained at the maximum pump power of
133.8 mW while the highest energy of 0.74 nJ was obtained at pump
power of 69.0 mW.
Abstract: The biomass-based fuels have become great concern in order to replace the petroleum-based fuels. Biofuels are a wide range of fuels referred to liquid, gas and solid fuels produced from biomass. Recently, higher chain alcohols such as 3-methyl-1-butanol and isobutanol have become a better candidate compared to bioethanol in order to replace gasoline as transportation fuel. Therefore, in this study, 3-methyl-1-butanol was produced through a fermentation process by yeast. Several types of yeast involved in this research including Saccharomyces cerevisiae, Kluyveromyces lactis GG799 and Pichia pastoris (KM71H, GS115 and X33). The result obtained showed that K. lactis GG799 gave the highest concentration of 3-methyl-1-butanol at 274 mg/l followed by S. cerevisiae, P. pastoris GS115, P. pastoris KM71H and P. pastoris X33 at 265 mg/l, 190 mg/l, 182 mg/l and 174 mg/l respectively. Based on the result, it proved that yeast have a potential in producing 3-methyl-1-butanol naturally.
Abstract: In the present work, detailed analysis on flow characteristics of a pair of immiscible liquids through horizontal pipeline is simulated by using ANSYS FLUENT 6.2. Moderately viscous oil and water (viscosity ratio = 107, density ratio = 0.89 and interfacial tension = 0.024 N/m) have been taken as system fluids for the study. Volume of Fluid (VOF) method has been employed by assuming unsteady flow, immiscible liquid pair, constant liquid properties, and co-axial flow. Meshing has been done using GAMBIT. Quadrilateral mesh type has been chosen to account for the surface tension effect more accurately. From the grid independent study, we have selected 47037 number of mesh elements for the entire geometry. Simulation successfully predicts slug, stratified wavy, stratified mixed and annular flow, except dispersion of oil in water, and dispersion of water in oil. Simulation results are validated with horizontal literature data and good conformity is observed. Subsequently, we have simulated the hydrodynamics (viz., velocity profile, area average pressure across a cross section and volume fraction profile along the radius) of stratified wavy and annular flow at different phase velocities. The simulation results show that in the annular flow, total pressure of the mixture decreases with increase in oil velocity due to the fact that pipe cross section is completely wetted with water. Simulated oil volume fraction shows maximum at the centre in core annular flow, whereas, in stratified flow, maximum value appears at upper side of the pipeline. These results are in accord with the actual flow configuration. Our findings could be useful in designing pipeline for transportation of crude oil.
Abstract: Abundant and cheap agricultural waste of oil palm trunk (OPT) juice was used to produce bioethanol. Two strains of Saccharomyces cerevisiae and a strain of Pichia stipitis were used to produce bioethanol from the OPT juice. Fermentation was conducted at previously optimized condition at 30oC and without shaking. The kinetic parameters were estimated and calculated. Monod equation and Hinshelwood model is used to relate the specific growth to the concentration of the limiting substrate and also to simulate bioethanol production rate. Among the three strains, single S. cerevisiae Kyokai no. 7 produce the highest ethanol yield of 0.477 g/l.h within the shortest time (12 h). This yeast also produces more than 20 g/l ethanol concentration within 10 h of fermentation.
Abstract: In this paper the vibration of a synchronous belt drive
during start-up is analyzed and discussed. Besides considering the
belt elasticity, the model here proposed also takes into consideration
the electromagnetic response of the DC motor. The solution of the
motion equations is obtained by means of the modal analysis in
state space, which allows to obtain the decoupling of all equations,
without introducing the hypothesis of proportional damping. The
mathematical model of the transmission and the solution algorithms
have been implemented within a computing software that allows the
user to simulate the dynamics of the system and to evaluate the effects
due to the elasticity of the belt branches and to the electromagnetic
behavior of the DC motor. In order to show the details of the
calculation procedure, the paper presents a case study developed with
the aid of the above-mentioned software.
Abstract: The Analytic Hierarchy Process is frequently used
approach for solving decision making problems. There exists wide
range of software programs utilizing that approach. Their main
disadvantage is that they are relatively expensive and missing
intermediate calculations. This work introduces a Microsoft Excel
add-in called DAME – Decision Analysis Module for Excel.
Comparing to other computer programs DAME is free, can work
with scenarios or multiple decision makers and displays intermediate
calculations. Users can structure their decision models into three
levels – scenarios/users, criteria and variants. Items on all levels can
be evaluated either by weights or pair-wise comparisons. There are
provided three different methods for the evaluation of the weights of
criteria, the variants as well as the scenarios – Saaty’s Method,
Geometric Mean Method and Fuller’s Triangle Method.
Multiplicative and additive syntheses are supported. The proposed
software package is demonstrated on couple of illustrating examples
of real life decision problems.
Abstract: This article deals with the perceived quality of regional products in the Moravian-Silesian region in the Czech Republic. Research was focused on finding out what do consumers perceive as a quality product and what characteristics make a quality product. The data were obtained by questionnaire survey andanalysed by IBM SPSS. From the thousands of respondents the representative sample of 719 for MS region was created based on demographic factors of gender, age, education and income. The research analysis disclosed that consumers in MS region are still price oriented and that the preference of quality over price does not depend on regional brand knowledge.
Abstract: Strontium hexaferrite (SrFe12O19; Sr-ferrite) is one of
the well-known materials for permanent magnets. In this study, Mtype
strontium ferrite was prepared by following the conventional
ceramic method from steelmaking by-product. Initial materials;
SrCO3 and by-product, were mixed together in the composition of
SrFe12O19 in different Sr/Fe ratios. The mixtures of these raw
materials were dry-milled for 6h. The blended powder was presintered
(i.e. calcination) at 1000°C for different times periods, then
cooled down to room temperature. These pre-sintered samples were
re-milled in a dry atmosphere for 1h and then fired at different
temperatures in atmospheric conditions, and cooled down to room
temperature. The produced magnetic powder has a dense hexagonal
grain shape structure. The calculated energy product values for the
produced samples ranged from 0.3 to 2.4 MGOe.
Abstract: The classroom of the 21st century is an ever changing forum for new and innovative thoughts and ideas. With increasing technology and opportunity, students have rapid access to information that only decades ago would have taken weeks to obtain. Unfortunately, new techniques and technology is not a cure for the fundamental problems that have plagued the classroom ever since education was established. Class size has been an issue long debated in academia. While it is difficult to pin point an exact number, it is clear that in this case more does not mean better. By looking into the success and pitfalls of classroom size the true advantages of smaller classes will become clear. Previously, one class was comprised of 50 students. Being seventeen and eighteen-year-old students, sometimes it was quite difficult for them to stay focused. To help them understand and gain much knowledge, a researcher introduced “The Theory of Multiple Intelligence” and this, in fact, enabled students to learn according to their own learning preferences no matter how they were being taught. In this lesson, the researcher designed a cycle of learning activities involving all intelligences so that everyone had equal opportunities to learn.
Abstract: The aim of this paper is to create a proposal for determining the costs of logistics processes by using process-oriented calculation methods. The traditional approach is that logistics costs are part of manufacturing overhead which is usually calculated as a percentage surcharge. Therefore in the traditional approach it is not obvious where and in which activities costs were incurred. So it is impossible to trace logistics costs to products. Our point of view is trying to fix or at least improve this issue. Another benefit of applying the process approach is identification of logistics processes which are otherwise part of manufacturing overhead. In the first part this paper describes the development of process-oriented methods over time. The next part shows the possibility of implementing the process-oriented method called Prozesskostenrechnung to logistics processes. The conclusion summarizes advantages and disadvantages of using this method in logistics.