Abstract: Anaerobic batch experiments were conducted to investigate the effect of magnetite-supplementation (7 mM) on methane production from digested sludge undergoing two different microbial growth phases, namely fresh sludge (exponential growth phase) and degassed sludge (endogenous decay phase). Three different particle sizes were assessed: small (50 - 150 nm), medium (168 – 490 nm) and large (800 nm - 4.5 µm) particles. Results show that, in the case of the fresh sludge, magnetite significantly enhanced the methane production rate (up to 32%) and reduced the lag phase (by 15% - 41%) as compared to the control, regardless of the particle size used. However, the cumulative methane produced at the end of the incubation was comparable in all treatment and control bottles. In the case of the degassed sludge, only the medium-sized magnetite particles increased significantly the methane production rate (12% higher) as compared to the control. Small and large particles had little effect on the methane production rate but did result in an extended lag phase which led to significantly lower cumulative methane production at the end of the incubation period. These results suggest that magnetite produces a clear and positive effect on methane production only when an active and balanced microbial community is present in the anaerobic digester. It is concluded that, (i) the effect of magnetite particle size on increasing the methane production rate and reducing lag phase duration is strongly influenced by the initial metabolic state of the microbial consortium, and (ii) the particle size would positively affect the methane production if it is provided within the nanometer size range.
Abstract: This study investigates the production of renewable energy (biogas) from biomedical hazard waste (blood) and eco-friendly disposal. Biogas is produced by the bacterial anaerobic digestion of biomaterial (blood). During digestion process bacterial feeding result in breaking down chemical bonds of the biomaterial and changing its features, by the end of the digestion (biogas production) the remains become manure as known. That has led to the economic and eco-friendly disposal of hazard biomedical waste (blood). The samples (Whole blood, Red blood cells 'RBCs', Blood platelet and Fresh Frozen Plasma ‘FFP’) are collected and measured in terms of carbon to nitrogen C/N ratio and total solid, then filled in connected flasks (three flasks) using water displacement method. The results of trails showed that the platelet and FFP failed to produce flammable gas, but via a gas analyzer, it showed the presence of the following gases: CO, HC, CO₂, and NOX. Otherwise, the blood and RBCs produced flammable gases: Methane-nitrous CH₃NO (99.45%), which has a blue color flame and carbon dioxide CO₂ (0.55%), which has red/yellow color flame. Methane-nitrous is sometimes used as fuel for rockets, some aircraft and racing cars.
Abstract: In this study, raw coffee wastewater (CWW) was used as a complex substrate for anaerobic digestion. The inoculum adaptation stage, microbial diversity analysis and biomethane potential (BMP) tests were performed. A fast inoculum adaptation stage was used by the replacement of vinasse to CWW in an anaerobic sequential batch reactor (AnSBR) operated at mesophilic conditions. Illumina MiSeq sequencing was used to analyze the microbial diversity. While, BMP tests using inoculum adapted to CWW were carried out at different inoculum to substrate (I/S) ratios (2:1, 3:1 and 4:1, on a VS basis). Results show that the adaptability percentage was increased gradually until it reaches the highest theoretical value in a short time of 10 d; with a methane yield of 359.10 NmL CH4/g COD-removed; Methanobacterium beijingense was the most abundant microbial (75%) and the greatest specific methane production was achieved at I/S ratio 4:1, whereas the lowest was obtained at 2:1, with BMP values of 320 NmL CH4/g VS and 151 NmL CH4/g VS, respectively. In conclusion, gradual replacement of substrate was a feasible method to adapt the inoculum in a short time even using complex raw substrates, whereas in the BMP tests, the specific methane production was proportional to the initial amount of inoculum.
Abstract: Extracting energy from biomass is an important alternative to produce different types of energy (heat, electricity, or both) assuring low pollution and better efficiency. It is a new yet reliable approach to reduce green gas emission by extracting methane from industry effluents and use it to power machinery. We focused in our project on using paper and mill effluents, treated in a UASB reactor. The methane produced is used in the factory’s power supply. The aim of this work is to develop an electronic system using Arduino platform connected to a gas sensor, to measure and display the curve of daily methane production on processing. The sensor will send the gas values in ppm to the Arduino board so that the later sends the RS232 hardware protocol. The code developed with processing will transform the values into a curve and display it on the computer screen.
Abstract: In this study, the anaerobic treatability of chicken manure diluted with tap water (with an influent feed ratio of 1 kg of fresh chicken manure to 6 liter of tap water) was investigated in a lab-scale anaerobic sludge bed (ASB) reactor inoculated with the granular sludge already adapted to chicken manure. The raw waste digested in this study was the manure from laying-hens having average total solids (TS) of about 30% with ca. 60% volatile content. The ASB reactor was fed semi-continuously at ambient operating temperature range (17-23◦C) at a HRT of 13 and 26 days for about 6 months, respectively. The respective average total and soluble chemical oxygen demand (COD) removals were ca. 90% and 75%, whereas average biomethane production rate was calculated ca. 180 lt per kg of CODremoved from the ASB reactor at an average HRT of 13 days. Moreover, total suspended solids (TSS) and volatile suspended solids (VSS) in the influent were reduced more than 97%. Hence, high removals of the organic compounds with respective biogas production made anaerobic stabilization of the diluted chicken manure by ASB reactor at ambient operating temperatures viable. By this way, external heating up to 35◦C (i.e. anaerobic processes have been traditionally operated at mesophilic conditions) could be avoided in the scope of this study.
Abstract: This study investigates the influence of low
temperature thermo-chemical pretreatment of organic food waste on
performance of COD solubilisation. Both temperature and alkaline
agent were reported to have effect on solubilizing any possible
biomass including organic food waste. The three independent
variables considered in this pretreatment were temperature (50-90oC),
pretreatment time (30-120 minutes) and alkaline concentration,
sodium hydroxide, NaOH (0.7-15 g/L). The maximal condition
obtained were 90oC, 15 g/L NaOH for 2 hours. Solubilisation has
potential in enhancing methane production by providing high amount
of soluble components at early stage during anaerobic digestion.
Abstract: Biological conversion of biomass to methane has
received increasing attention in recent years. Grasses have been
explored for their potential anaerobic digestion to methane. In this
review, extensive literature data have been tabulated and classified.
The influences of several parameters on the potential of these
feedstocks to produce methane are presented. Lignocellulosic
biomass represents a mostly unused source for biogas and ethanol
production. Many factors, including lignin content, crystallinity of
cellulose, and particle size, limit the digestibility of the hemicellulose
and cellulose present in the lignocellulosic biomass. Pretreatments
have used to improve the digestibility of the lignocellulosic biomass.
Each pretreatment has its own effects on cellulose, hemicellulose and
lignin, the three main components of lignocellulosic biomass. Solidstate
anaerobic digestion (SS-AD) generally occurs at solid
concentrations higher than 15%. In contrast, liquid anaerobic
digestion (AD) handles feedstocks with solid concentrations between
0.5% and 15%. Animal manure, sewage sludge, and food waste are
generally treated by liquid AD, while organic fractions of municipal
solid waste (OFMSW) and lignocellulosic biomass such as crop
residues and energy crops can be processed through SS-AD. An
increase in operating temperature can improve both the biogas yield
and the production efficiency, other practices such as using AD
digestate or leachate as an inoculant or decreasing the solid content
may increase biogas yield but have negative impact on production
efficiency. Focus is placed on substrate pretreatment in anaerobic
digestion (AD) as a means of increasing biogas yields using today’s
diversified substrate sources.
Abstract: In this study, we illustrated the performance and
microbial community of single- and two-phase systems anaerobically
co-digesting cassava pulp and pig manure. The results showed that
the volatile solid reduction and biogas productivity of two-phase
CSTR were 66 ± 4% and 2000 ± 210 ml l-1 d-1, while those of singlephase
CSTR were 59 ± 1% and 1670 ± 60 ml l-1 d-1, respectively. Codigestion
in two-phase CSTR gave higher 12% solid degradation and
25% methane production than single-phase CSTR. Phylogenetic
analysis of 16S rDNA clone library revealed that the Bacteroidetes
were the most abundant group, followed by the Clostridia in singlephase
CSTR. In hydrolysis/acidification reactor of two-phase system,
the bacteria within the phylum Firmicutes, especially Clostridium,
Eubacteriaceae and Lactobacillus were the dominant phylogenetic
groups. Among the Archaea, Methanosaeta sp. was the exclusive
predominant in both digesters while the relative abundance of
Methanosaeta sp. and Methanospirillum hungatei differed between
the two systems.
Abstract: This paper describes technological possibilities to
enhance methane productionin the anaerobic stabilization of wastewater treatment plant excess sludge. This objective can be achieved by the addition of waste residues: crude glycerol from biodiesel production and residues from fishery. The addition
ofglycerol in an amount by weight of 2 – 5% causes enhancement of methane production of about 250 – 400%. At the same time the
percentage increase of total solids concentration in the outgoing sludge is ten or more times less. The containment of methane in
biogas is higher in case of admixed substrate.
Abstract: In this research, an anaerobic co-digestion using decanter cake from palm oil mill industry to improve the biogas production from frozen seafood wastewater is studied using Continuously Stirred Tank Reactor (CSTR) process. The experiments were conducted in laboratory-scale. The suitable Hydraulic Retention Time (HRT) was observed in CSTR experiments with 24 hours of mixing time using the mechanical mixer. The HRT of CSTR process impacts on the efficiency of biogas production. The best performance for biogas production using CSTR process was the anaerobic codigestion for 20 days of HRT with the maximum methane production rate of 1.86 l/d and the average maximum methane production of 64.6%. The result can be concluded that the decanter cake can improve biogas productivity of frozen seafood wastewater.