Abstract: This study aims to screen out and to optimize the
major nutrients for maximum carotenoid production and
antioxidation characteristics by Rhodotorula rubra. It was found that
supplementary of 10 g/l glucose as carbon source, 1 g/l ammonium
sulfate as nitrogen source and 1 g/l yeast extract as growth factor in
the medium provided the better yield of carotenoid content of 30.39
μg/g cell dry weight the amount of antioxidation of Rhodotorula
rubra by DPPH, ABTS and MDA method were 1.463%, 34.21% and
34.09 μmol/l, respectively.
Abstract: The White Kwao (Pueraria mirifica), a potent phytoestrogenic medicinal plant, has long been use in Thailand as a traditional folkmedicine. However, no scientific information of the direct effect of White Kwao on the development of mammalian embryo was available. Therefore, the purpose of this study was to investigate the effect of White Kwao extract on the in vitro development and implantation rate of mouse embryos. This study was designed into two experiments. In the first experiment, the two-cell stage mouse embryos were collected from the oviduct of superovulated mature female mice, and randomly cultured in three different media, the M16, M16 supplemented with 0.52μg esthinylestradiol-17β, and M16 supplemented with 10 mg/ml White Kwao extract. The culture was incubated in CO2 incubator at 37 oC . After the embryos were cultivated, the developments of embryos were observed every 24 hours for 5 days. The development rate of embryos from the two-cell stage to blastocyst stage in the media was with White Kwao was significantly higher (p
Abstract: With high speed vessels getting ever more sophisti-cated, travelling at higher and higher speeds and operating in With high speed vessels getting ever more sophisticated,
travelling at higher and higher speeds and operating in areas of
high maritime traffic density, training becomes of the highest priority
to ensure that safety levels are maintained, and risks are adequately
mitigated. Training onboard the actual craft on the actual route still
remains the most effective way for crews to gain experience. However,
operational experience and incidents during the last 10 years
demonstrate the need for supplementary training whether in the area
of simulation or man to man, man/ machine interaction. Training and
familiarisation of the crew is the most important aspect in preventing
incidents. The use of simulator, computer and web based training
systems in conjunction with onboard training focusing on critical
situations will improve the man machine interaction and thereby
reduce the risk of accidents. Today, both ship simulator and bridge
teamwork courses are now becoming the norm in order to improve
further emergency response and crisis management skills. One of the
main causes of accidents is the human factor. An efficient way to
reduce human errors is to provide high-quality training to the personnel
and to select the navigators carefully.areas of high maritime traffic density, training becomes of the highest priority to ensure that safety levels are maintained, and risks are adequately mitigated. Training onboard the actual craft on the actual route still remains the most effective way for crews to gain experience. How-ever, operational experience and incidents during the last 10 years demonstrate the need for supplementary training whether in the area of simulation or man to man, man/ machine interaction. Training and familiarisation of the crew is the most important aspect in preventing incidents. The use of simulator, computer and web based training systems in conjunction with onboard training focusing on critical situations will improve the man machine interaction and thereby reduce the risk of accidents. Today, both ship simulator and bridge teamwork courses are now becoming the norm in order to improve further emergency response and crisis management skills. One of the main causes of accidents is the human factor. An efficient way to reduce human errors is to provide high-quality training to the person-nel and to select the navigators carefully. KeywordsCBT - WBT systems, Human factors.
Abstract: Natural pozzolan (NP) is one of the potential
prehistoric alternative binders in the construction industry. It has
been investigated as cement replacement in ordinary concrete by
several researchers for many purposes. Various supplementary
cementitious materials (SCMs) such as fly ash, limestone dust and
silica fume are widely used in the production of SCC; however,
limited studies to address the effect of NP on the properties of SCC
are documented. The current research is composed of different SCC
paste and concrete mixtures containing different replacement levels
of local NP as an alternative SCM. The effect of volume of paste
containing different amounts of local NP related to W/B ratio and
cement content on SCC fresh properties was assessed. The variations
in the fresh properties of SCC paste and concrete represented by
slump flow (flowability) and the flow rate were determined and
discussed. The results indicated that the flow properties of SCC paste
and concrete mixtures, at their optimized superplasticizer dosages,
were affected by the binder content of local NP and the total volume
fraction of SCC paste.
Abstract: In most wheat growing moderate regions and
especially in the north of Iran climate, is affected grain filling by
several physical and abiotic stresses. In this region, grain filling often
occurs when temperatures are increasing and moisture supply is
decreasing. The experiment was designed in RCBD with split plot
arrangements with four replications. Four irrigation treatments
included (I0) no irrigation (check); (I1) one irrigation (50 mm) at
heading stage; (I2) two irrigation (100 mm) at heading and anthesis
stage; and (I3) three irrigation (150 mm) at heading, anthesis and
early grain filling growth stage, two wheat cultivars (Milan and
Shanghai) were cultured in the experiment. Totally raining was 453
mm during the growth season. The result indicated that biological
yield, grain yield and harvest index were significantly affected by
irrigation levels. I3 treatment produced more tillers number in m2,
fertile tillers number in m2, harvest index and biological yield. Milan
produced more tillers number in m2, fertile tillers in m2, while
Shanghai produced heavier tillers and grain 1000 weight. Plant height
was significant in wheat varieties while were not statistically
significant in irrigation levels. Milan produced more grain yield,
harvest index and biological yield. Grain yield shown that I1, I2, and
I3 produced increasing of 5228 (21%), 5460 (27%) and 5670 (29%)
kg ha-1, respectively. There was an interaction of irrigation and
cultivar on grain yields. In the absence of the irrigation reduced grain
1000 weight from 45 to 40 g. No irrigation reduced soil moisture
extraction during the grain filling stage. Current assimilation as a
source of carbon for grain filling depends on the light intercepting
viable green surfaces of the plant after anthesis that due to natural
senescence and the effect of various stresses. At the same time the
demand by the growing grain is increasing. It is concluded from
research work that wheat crop irrigated Milan cultivar could increase
the grain yield in comparison with Shanghai cultivar. Although, the
grain yield of Shanghai under irrigation was slightly lower than
Milan. This grain yield also was related to weather condition, sowing
date, plant density and location conditions and management of
fertilizers, because there was not significant difference in biological
and straw yield. The best result was produced by I1 treatment. I2 and
I3 treatments were not significantly difference with I1 treatment.
Grain yield of I1 indicated that wheat is under soil moisture
deficiency. Therefore, I1 irrigation was better than I0.
Abstract: The cDNA encoding the 326 amino acids of a Class I
basic chitinase gene from Leucaena leucocephala de Wit (KB3,
Genbank accession: AAM49597) was cloned under the control of
CaMV35S promoter in pCAMBIA 1300 and transferred to
Koshihikari. Calli of Koshihikari rice was transformed with
agrobacterium with this construct expressing the chitinase and β-
glucouronidase (GUS). The frequencies of calli 90 % has been
obtained from rice seedlings cultured on NB medium. The high
regeneration frequencies, 74% was obtained from calli cultured on
regeneration medium containing 4 mg/l BAP, and 7 g/l phytagel at
25°C. Various factors were studied in order to establish a procedure
for the transformation of Koshihikari Agrobacterium tumefaciens.
Supplementation of 50 mM acetosyringone to the medium during
coculivation was important to enhance the frequency to transient
transformation. The 4 week-old scutellum-derived calli were
excellent starting materials. Selection medium based on NB medium
supplement with 40 mg/l hygromycin and 400 mg/l cefotaxime were
an optimized medium for selection of transformed rice calli. The
percentage of transformation 70 was obtained. Recombinant calli and
regenerated rice plants were checked the expression of chitinase and
gus by PCR, northern blot gel, southern blot gel, and gus assay.
Chitinase and gus were expressed in all parts of recombinant rice.
The rice line expressing the KB3 chiitnase was more resistant to the
blast fungus Fusarium monoliforme than control line.
Abstract: The effects of chitosan, a biodegradable polymer,
were studied in Grammatophyllum speciosum protocorm-like bodies
(PLBs) in vitro culture. The chitosan concentration of 0, 5, 10, 15,
20, 25, 50 or 100 mg/l were supplemented in half-strength Murashige
and Skoog (1/2 MS) liquid or on agar media containing 2% (w/v)
sucrose. The results showed that liquid medium supplemented with
15 mg/l chitosan showed the highest relative growth rate (7-fold
increase) of PLBs. On 1/2 MS agar medium supplemented with 25
mg/l chitosan gave the highest relative growth rate (4-fold increase).
The relative growth rate of G. speciosum PLBs on agar medium was
significantly lower than that in liquid medium. Moreover, chitosan,
supplemented to agar medium promoted shoot formation but not
rooting. However, supplementation at too high a level, such as 100
mg/l can inhibit growth and kill PLBs.
Abstract: The purpose of this study was to assess the value of Second Life among post-secondary instructors with experience using Second Life as an educational tool. Using Everett Rogers-s diffusion of innovations theory, survey respondents (N = 162), were divided into three adopter categories: innovators, early adopters and the early majority. Respondents were from 15 countries and 25 academic disciplines, indicating the considerable potential this innovation has to be adopted across many different borders and in many areas of academe. Nearly 94% of respondents said they plan to use Second Life again as an educational tool. However, no significant differences were found in instructors- levels of satisfaction with Second Life as an educational tool or their perceived effect on student learning across adopter categories. On the other hand, instructors who conducted class fully in Second Life were significantly more satisfied than those who used Second Life as only a small supplement to a real-world class. Overall, personal interest factors, rather than interpersonal communication factors, most influenced respondents- decision to adopt Second Life as an educational tool. In light of these findings, theoretical implications are discussed and practical suggestions are provided.
Abstract: This study was designed to determine effect of
supplemented tomato pomace and fobrolytic enzyme on egg
production and egg quality. A total of 40 CP brown laying hens (95
week old) were used in completely randomized design in 2x2
factorial arrangement with or without enzyme supplementation. Four
dietary treatments included: Control (C), Fibrolytic enzyme (FE),
10% Tomato pomace (TP), and Fibrolytic enzyme + 10 % Tomato
pomace (FE+TP). Each of the four dietary treatments was fed up to
30 days (10 birds/treatment). Live performance, egg production, egg
weight and quality were determined for whole period. Dietary
treatments had no effect (P>0.05) on live performance, egg weight,
yolk color, and egg production. Therefore, laying hens fed diets with
fibrolytic enzyme were significantly (P
Abstract: Martingale model diagnostic for assessing the fit of logistic regression model to recurrent events data are studied. One way of assessing the fit is by plotting the empirical standard deviation of the standardized martingale residual processes. Here we used another diagnostic plot based on martingale residual covariance. We investigated the plot performance under several types of model misspecification. Clearly the method has correctly picked up the wrong model. Also we present a test statistic that supplement the inspection of the two diagnostic. The test statistic power agrees with what we have seen in the plots of the estimated martingale covariance.
Abstract: In many industries, control charts is one of the most
frequently used tools for quality management. Hotelling-s T2 is used
widely in multivariate control chart. However, it has little defect when
detecting small or medium process shifts. The use of supplementary
sensitizing rules can improve the performance of detection. This study
applied sensitizing rules for Hotelling-s T2 control chart to improve the
performance of detection. Support vector machines (SVM) classifier
to identify the characteristic or group of characteristics that are
responsible for the signal and to classify the magnitude of the mean
shifts. The experimental results demonstrate that the support vector
machines (SVM) classifier can effectively identify the characteristic
or group of characteristics that caused the process mean shifts and the
magnitude of the shifts.
Abstract: In this paper, a new efficient method for load balancing in low voltage distribution systems is presented. The proposed method introduces an improved Leap-frog method for optimization. The proposed objective function includes the difference between three phase currents, as well as two other terms to provide the integer property of the variables; where the latter are the status of the connection of loads to different phases. Afterwards, a new algorithm is supplemented to undertake the integer values for the load connection status. Finally, the method is applied to different parts of Tabriz low voltage network, where the results have shown the good performance of the proposed method.
Abstract: Sorghum flour was supplemented with 15 and 30%
chickpea flour. Sorghum flour and the supplement were fermented at
35 oC for 0, 8, 16, and 24 h. Changes in pH, titrable acidity, total
soluble solids, protein content, in vitro protein digestibility and
amino acid composition were investigated during fermentation and/or
after supplementation of sorghum flour with chickpea. The pH of the
fermenting material decreased sharply with a concomitant increase in
the titrable acidity. The total soluble solids remained unchanged with
progressive fermentation time. The protein content of sorghum
cultivar was found to be 9.27 and that of chickpea was 22.47%. The
protein content of sorghum cultivar after supplementation with15 and
30% chickpea was significantly (P ≤ 0.05) increased to 11.78 and
14.55%, respectively. The protein digestibility also increased after
fermentation from 13.35 to 30.59 and 40.56% for the supplements,
respectively. Further increment in protein content and digestibility
was observed when supplemented and unsupplemented samples were
fermented for different periods of time. Cooking of fermented
samples was found to increase the protein content slightly and
decreased digestibility for both supplements. Amino acid content of
fermented and fermented and cooked supplements was determined.
Supplementation was found to increase the lysine and therionine
content. Cooking following fermentation decreased lysine,
isoleucine, valine and sulfur containg amino acids.
Abstract: This paper presents the outcomes of a qualitative
study which aims to investigate the pedagogical potentials of serious
games in the preparation of future teachers. The authors discuss the
existing problems and barriers associated with the organization of
teaching practices in Bulgaria as part of the pre-service teacher
training, as well as the attitudes and perceptions of the interviewed
academics, teachers and trainees concerning the integration of serious
games in the teaching practicum. The study outcomes strongly
confirm the positive attitudes of the respondents to the introduction
of virtual learning environments for the development of professional
skills of future teachers as a supplement to the traditional forms of
education. Through the inclusion of serious games it is expected to
improve the quality of practical training of pre-service teachers as
they overcome many of the problems identified in the existing
teaching practices. The outcomes of the study will inform the design
of the educational simulation software which is part of the project
SimAula Tomorrow's Teachers Training.
Abstract: Shoots, with three leaves, of Paphiopedilum 'Delrosi'
were used as explants for multiple shoot induction. Modified
Hyponex medium was supplemented with thidiazuron (TDZ), N6-
benzyladenine (BA) or kinetin (Kn) alone and in combinations with
2,4-dichlorophenoxyacetic acid (2,4-D). All explants were cultured
for 15 weeks. It was found that TDZ alone at the concentration of
0.45μM or in combination with 4.52μM 2,4-D and 8.88μM BA in
combination with 13.56μM 2,4-D promoted multiple shoots. The
highest shoot sprouting efficiencies (80.0, 90.0 and 80.0%) and new
shoot numbers (1.5, 1.3 and 1.1) were obtained, respectively. Fresh
weight, height, numbers of leaf and root of new shoots and initial
explants were discussed.
Abstract: Chest pain is one of the most prevalent complaints
among adults that cause the people to attend to medical centers. The
aim was to determine the prevalence and risk factors of chest pain
among over 30 years old people in Tehran. In this cross-sectional
study, 787 adults took part from Apr 2005 until Apr 2006. The
sampling method was random cluster sampling and there were 25
clusters. In each cluster, interviews were performed with 32 over 30
years old, people lived in those houses. In cases with chest pain, extra
questions asked. The prevalence of CP was 9% (71 cases). Of them
21 cases (6.5%) were in 41-60 year age ranges and the remainders
were over 61 year old. 19 cases (26.8%) mentioned CP in resting
state and all of the cases had exertion onset CP. The CP duration was
10 minutes or less in all of the cases and in most of them (84.5%), the
location of pain mentioned left anterior part of chest, left anterior part
of sternum and or left arm. There was positive history of myocardial
infarction in 12 cases (17%). There was significant relation between
CP and age, sex and between history of myocardial infarction and
marital state of study people. Our results are similar to other studies-
results in most parts, however it is necessary to perform
supplementary tests and follow up studies to differentiate between
cardiac and non-cardiac CP exactly.
Abstract: A feeding trial was conducted to investigate the effect
of periodically use of garlic on performance and carcass
characteristics in broiler chickens. 240 1-day-old Ross broiler chicks
randomly allocated into the 10 dietary treatments (A, B, C, D, E, F,
G, H, I and J) for 6 wk. Treatment A or control group, received basal
diet (based on standards of Ross management guidelines) without
supplementation of garlic powder while B, C and D dietary
treatments were basal diet supplemented with 0.5, 1 and 3% garlic
powder, respectively for the whole time of experiment (6 weeks).
Birds in group E, F and G were fed control diet supplemented with
0.5, 1 and 3% garlic powder, respectively just in their starter diet (0-
21d). Birds in three other treatments (H, I and J) received control diet
for the first 21 days and 0.5, 1 and 3% of garlic powder was added to
their finisher diets, respectively. 1 and 3% supplemented groups in
finisher period had better performance as compared with other
groups. Since present study conducted in optimum and antiseptic
conditions, it seems that better or more responses could be expected
in performance if the raising conditions would not be healthy.
Abstract: For sterilization of Phalaenopsis culture medium without autoclaving, selected single sterilizing agents and in combinations were added to a 25ml Hyponex medium in a 120ml glass container. Treated liquid and solid media, supplemented with sterilizing agents, were compared to a control medium, autoclaved at 121°C for 15min. It was found that 90(L of 10% povidone-iodine, 150(L of 5.25% sodium hypochlorite, 150(L of 2% mercurochrome, 90(L of 2.5% iodine + 2.5% potassium iodine in combination with 10% providone-iodine (1:3) and 30(L of 2.5% iodine + 2.5% potassium iodide in combination with 2% mercurochrome showed 100% sterile conditions in liquid medium but provided 75, 100, 50, 75 and 80% sterile conditions, respectively, in solid medium. For growth of Phalaenopsis protocorms, 90(L of 10% povidone-iodine in liquid Hyponex medium gave the comparable growth of protocorms to control medium while 150(L of 5.25% sodium hypochlorite in solid medium provided the promising growth of protocorms. Growth of protocorms, whole fresh weight, numbers of leaf and root, root length and number of protocorm-like bodies, was discussed.
Abstract: The effect of cassava root ensiled with cassava top or
legumes on voluntary feed intake and milk production were
determined in 12 dairy cows using a 4×3 change-over design.
Experimental period were 30 days long and consisted of 14 days of
adaptation. Silage was prepared from cassava root mixed with
cassava top or legumes at ratio 60:40. Cows were allotted at random
to receive ad libitum one of four rations: T1) control, T2) cassava
root +cassava top-silages, T3) cassava root +hamata - silages and T4)
cassava root +Thapra stylo-silages.
The dry matter intake (BW0.75) was higher (P< 0.05) in cow fed
with silages diets compared with T1. However, the intake of T2 was
higher among treatments. Milk production was lowest in cow fed
with T1. Among silages based diets, milk production was not
significantly different but 4%FCM was higher in cow fed T2. Milk
compositions were not affected by feeding diets.
It is concluded that feeding cassava root ensiled with its leaves as
a supplement increased dry matter intake and significantly improved
4%FCM. The combination of cassava root and legume silages did not
improve the feed intake but did increase the milk production.
Abstract: In vitro plant regeneration has been successfully obtained from basal shoot explant of Vetiveria zizanioides through indirect organogenesis. The explant was cultured in Murashige & Skoog’s (MS) media supplemented with 2,4-D, IAA, and kinetin in various concentrations. Callus was well induced in media supplemented with 2 ppm 2,4-D, 1 ppm IAA, and 1 ppm kinetin. This callus was then transferred to MS media supplemented with 1 - 5 ppm of BAP for shoot regeneration. The media supplemented with 3 ppm BAP was a suitable medium for shoot induction, as well as for shoot multiplication. Rooting was well developed in shoot following transferred to half MS media containing 0.2 ppm IBA. Plantlet was then transferred to husk charcoal for acclimatization, and almost all (90%) of plantlets were survived during acclimatization.