Multiple Shoot Formation of Paphiopedilum 'Delrosi'
Shoots, with three leaves, of Paphiopedilum 'Delrosi'
were used as explants for multiple shoot induction. Modified
Hyponex medium was supplemented with thidiazuron (TDZ), N6-
benzyladenine (BA) or kinetin (Kn) alone and in combinations with
2,4-dichlorophenoxyacetic acid (2,4-D). All explants were cultured
for 15 weeks. It was found that TDZ alone at the concentration of
0.45μM or in combination with 4.52μM 2,4-D and 8.88μM BA in
combination with 13.56μM 2,4-D promoted multiple shoots. The
highest shoot sprouting efficiencies (80.0, 90.0 and 80.0%) and new
shoot numbers (1.5, 1.3 and 1.1) were obtained, respectively. Fresh
weight, height, numbers of leaf and root of new shoots and initial
explants were discussed.
[1] H. Koopowitz, Tropical slipper orchids: Paphiopedilum and
Phragmipedium species and hybrids. TimberPress Inc., Oregon,
2008.
[2] L. Yung, and L. Nean, L. 2001. "Effect of capsule maturation,
medium composition and suspension culture on in vitro germination
of Paphiopedilum primulinum seed." Journal of the Chinese Society
for Horticultural Science, vol. 47, pp. 147-156, 2001.
[3] Y. H. Lin, D. Jun, L. Chen, and C. Z. Lin, C. Z. 2004. "Seed
germination in vitro of Paphiopedilum armeniacum and P.
micranthum." Acta Horticulturae Sinica, vol. 31, pp. 540-542, 2004.
[4] C. M. Sama, and M. Kalita, "In vitro propagation of a terrestrial
endangered orchid Paphiopedilum insigne (Wall. Ex. Lindl) Pfitz."
Journal of Phytological Research, vol. 17, pp. 1-5, 2004.
[5] B. Long, A. X. Niemiera, and Z. Y. Chen, "In vitro propagation of
four threatened Paphiopedilum species (Orchidaceae)." Plant Cell,
Tissue and Organ Culture, vol. 101, pp. 151-162, 2010.
[6] P. I. Hong, J. T. Chen, and W. C. Chang, "Plant regeneration via
protocorm-like body formation and shoot multiplication from seedderived
callus of a maudiae type slipper orchid." Acta Physiologia
Plantarum, vol. 30, pp. 755-759, 2008.
[7] C. Y. Ng, and N. M. Saleh, "In vitro propagation of Paphiopedilum
orchid through formation of protocorm-like bodies." Plant Cell,
Tissue and Organ Culture, vol. 105, pp. 193-202, 2011.
[8] T. Y. Chen, J. T. Chen, and W. C. Chang, "Multiple shoot formation
and plant regeneration from stem nodal explants of Paphiopedilum
orchids." In Vitro Cellular & Developmental Biology−Plant, vol. 38,
pp. 595-597, 2002.
[9] C. Y. Ng, and N. M. Saleh, "In vitro multiplication of the rare and
endangered slipper orchid, Paphiopedilum rothschildianum
(orchidaceae). African Journal of Biotechnology, vol. 9, pp. 2062-
2068, 2010.
[10] T. Y. Chen, J. T. Chen, and C. W. Chang, "Plant regeneration
through direct shoot bud formation from leaf cultures of
Paphiopedilum orchids." Plant Cell, Tissue and Organ Culture, vol.
76, pp. 11-15, 2004.
[11] E. Nisayan, C. Thepsithar, and A. Thongpukdee, "Multiple shoot
induction from seed derived shoots of Paphiopedilum." Acta
Hortticulturae, vol. 878, pp. 199-204, 2010.
[12] D. B. Duncan, "Multiple range and multiple F test." Biometrics, vol.
11, pp. 1-42, 1955.
[13] T. Murashige, and F. Skoog, "A revised medium for rapid growth
and bioassays with tobacco tissue culture." Physiologia Plantarum,
vol. 15, pp. 473-497, 1962.
[14] Y. Lee, and N. Lee, "Plant regeneration from protocorm-derived
callus of Cyperidium formosanum." In Vitro Cellular &
Developmental Biology−Plant, vol. 39, pp. 475-479, 2003.
[15] H. Shimura, and Y. Koda, "Micropropagation of Cyperidium
macranthos var. rebunense through protocorm-like bodies derived
from mature seeds." Plant Cell, Tissue and Organ Culture, vol. 78,
pp. 273--276, 2004.
[16] L. Huang, C. Lin, C. Kuo, B. Huang, and T. Murashige,
"Paphiopedilum cloning in vitro." Scientia Horticulturae, vol. 91,
pp. 111-121, 2001.
[1] H. Koopowitz, Tropical slipper orchids: Paphiopedilum and
Phragmipedium species and hybrids. TimberPress Inc., Oregon,
2008.
[2] L. Yung, and L. Nean, L. 2001. "Effect of capsule maturation,
medium composition and suspension culture on in vitro germination
of Paphiopedilum primulinum seed." Journal of the Chinese Society
for Horticultural Science, vol. 47, pp. 147-156, 2001.
[3] Y. H. Lin, D. Jun, L. Chen, and C. Z. Lin, C. Z. 2004. "Seed
germination in vitro of Paphiopedilum armeniacum and P.
micranthum." Acta Horticulturae Sinica, vol. 31, pp. 540-542, 2004.
[4] C. M. Sama, and M. Kalita, "In vitro propagation of a terrestrial
endangered orchid Paphiopedilum insigne (Wall. Ex. Lindl) Pfitz."
Journal of Phytological Research, vol. 17, pp. 1-5, 2004.
[5] B. Long, A. X. Niemiera, and Z. Y. Chen, "In vitro propagation of
four threatened Paphiopedilum species (Orchidaceae)." Plant Cell,
Tissue and Organ Culture, vol. 101, pp. 151-162, 2010.
[6] P. I. Hong, J. T. Chen, and W. C. Chang, "Plant regeneration via
protocorm-like body formation and shoot multiplication from seedderived
callus of a maudiae type slipper orchid." Acta Physiologia
Plantarum, vol. 30, pp. 755-759, 2008.
[7] C. Y. Ng, and N. M. Saleh, "In vitro propagation of Paphiopedilum
orchid through formation of protocorm-like bodies." Plant Cell,
Tissue and Organ Culture, vol. 105, pp. 193-202, 2011.
[8] T. Y. Chen, J. T. Chen, and W. C. Chang, "Multiple shoot formation
and plant regeneration from stem nodal explants of Paphiopedilum
orchids." In Vitro Cellular & Developmental Biology−Plant, vol. 38,
pp. 595-597, 2002.
[9] C. Y. Ng, and N. M. Saleh, "In vitro multiplication of the rare and
endangered slipper orchid, Paphiopedilum rothschildianum
(orchidaceae). African Journal of Biotechnology, vol. 9, pp. 2062-
2068, 2010.
[10] T. Y. Chen, J. T. Chen, and C. W. Chang, "Plant regeneration
through direct shoot bud formation from leaf cultures of
Paphiopedilum orchids." Plant Cell, Tissue and Organ Culture, vol.
76, pp. 11-15, 2004.
[11] E. Nisayan, C. Thepsithar, and A. Thongpukdee, "Multiple shoot
induction from seed derived shoots of Paphiopedilum." Acta
Hortticulturae, vol. 878, pp. 199-204, 2010.
[12] D. B. Duncan, "Multiple range and multiple F test." Biometrics, vol.
11, pp. 1-42, 1955.
[13] T. Murashige, and F. Skoog, "A revised medium for rapid growth
and bioassays with tobacco tissue culture." Physiologia Plantarum,
vol. 15, pp. 473-497, 1962.
[14] Y. Lee, and N. Lee, "Plant regeneration from protocorm-derived
callus of Cyperidium formosanum." In Vitro Cellular &
Developmental Biology−Plant, vol. 39, pp. 475-479, 2003.
[15] H. Shimura, and Y. Koda, "Micropropagation of Cyperidium
macranthos var. rebunense through protocorm-like bodies derived
from mature seeds." Plant Cell, Tissue and Organ Culture, vol. 78,
pp. 273--276, 2004.
[16] L. Huang, C. Lin, C. Kuo, B. Huang, and T. Murashige,
"Paphiopedilum cloning in vitro." Scientia Horticulturae, vol. 91,
pp. 111-121, 2001.
@article{"International Journal of Biological, Life and Agricultural Sciences:53078", author = "Aree Thongpukdee and Ekasit Nisayan and Chockpisit Thepsithar", title = "Multiple Shoot Formation of Paphiopedilum 'Delrosi'", abstract = "Shoots, with three leaves, of Paphiopedilum 'Delrosi'
were used as explants for multiple shoot induction. Modified
Hyponex medium was supplemented with thidiazuron (TDZ), N6-
benzyladenine (BA) or kinetin (Kn) alone and in combinations with
2,4-dichlorophenoxyacetic acid (2,4-D). All explants were cultured
for 15 weeks. It was found that TDZ alone at the concentration of
0.45μM or in combination with 4.52μM 2,4-D and 8.88μM BA in
combination with 13.56μM 2,4-D promoted multiple shoots. The
highest shoot sprouting efficiencies (80.0, 90.0 and 80.0%) and new
shoot numbers (1.5, 1.3 and 1.1) were obtained, respectively. Fresh
weight, height, numbers of leaf and root of new shoots and initial
explants were discussed.", keywords = "Paphiopedilum, terrestrial orchids, in vitro culture, micropropagation, multiple shoot induction", volume = "7", number = "6", pages = "380-4", }
