Abstract: Agriculture is the backbone of economy of Pakistan
and cotton is the major agricultural export and supreme source of raw
fiber for our textile industry. To combat severe problems of insect
and weed, combination of three genes namely Cry1Ac, Cry2A and
EPSPS genes was transferred in locally cultivated cotton variety
MNH-786 with the use of Agrobacterium mediated genetic
transformation. The present study focused on the molecular screening
of transgenic cotton plants at T3 generation in order to confirm
integration and expression of all three genes (Cry1Ac, Cry2A and
EPSP synthase) into the cotton genome. Initially, glyphosate spray
assay was used for screening of transgenic cotton plants containing
EPSP synthase gene at T3 generation. Transgenic cotton plants which
were healthy and showed no damage on leaves were selected after 07
days of spray. For molecular analysis of transgenic cotton plants in
the laboratory, the genomic DNA of these transgenic cotton plants
were isolated and subjected to amplification of the three genes. Thus,
seventeen out of twenty (Cry1Ac gene), ten out of twenty (Cry2A
gene) and all twenty (EPSP synthase gene) were produced positive
amplification. On the base of PCR amplification, ten transgenic plant
samples were subjected to protein expression analysis through
ELISA. The results showed that eight out of ten plants were actively
expressing the three transgenes. Real-time PCR was also done to
quantify the mRNA expression levels of Cry1Ac and EPSP synthase
gene. Finally, eight plants were confirmed for the presence and active
expression of all three genes at T3 generation.
Abstract: This paper describes an analysis of Yacht Simulator
international trends and also explains about Yacht. The results are
summarized as follows. Attached to the cockpit are sensors that feed
-back information on rudder angle, boat heel angle and mainsheet
tension to the computer. Energy expenditure of the sailor measure
indirectly using expired gas analysis for the measurement of VO2 and
VCO2. At sea course configurations and wind conditions can be preset
to suit any level of sailor from complete beginner to advanced sailor.
Abstract: The air transport impact on environment is more than
ever a limitative obstacle to the aeronautical industry continuous
growth. Over the last decades, considerable effort has been carried
out in order to obtain quieter aircraft solutions, whether by changing
the original design or investigating more silent maneuvers. The
noise propagated by rotating surfaces is one of the most important
sources of annoyance, being present in most aerial vehicles. Bearing
this is mind, CEIIA developed a new computational chain for
noise prediction with in-house software tools to obtain solutions in
relatively short time without using excessive computer resources. This
work is based on the new acoustic tool, which aims to predict the
rotor noise generated during steady and maneuvering flight, making
use of the flexibility of the C language and the advantages of GPU
programming in terms of velocity. The acoustic tool is based in the
Formulation 1A of Farassat, capable of predicting two important
types of noise: the loading and thickness noise. The present work
describes the most important features of the acoustic tool, presenting
its most relevant results and framework analyses for helicopters and
UAV quadrotors.
Abstract: The objective of this study was to identify the optimal
level of partial replacement of Portland cement by the ashes
originating from burning straw and bagasse from sugar cane (ASB).
Order to this end, were made five series of flat plates and cylindrical
bodies: control and others with the partial replacement in 20, 30, 40
and 50% of ASB in relation to the mass of the Ordinary Portland
cement, and conducted a mechanical testing of simple axial
compression (cylindrical bodies) and the four-point bending (flat
plates) and determined water absorption (WA), bulk density (BD)
and apparent void volume (AVV) on both types of specimens. Based
on the data obtained, it may be noted that the control treatment
containing only Portland cement, obtained the best results. However,
the cylindrical bodies with 20% ashes showed better results
compared to the other treatments. And in the formulations plates, the
treatment which showed the best results was 30% cement
replacement by ashes.
Abstract: This paper examines how “Zakat” provides fair
income redistribution and aids the struggle against poverty. Providing
fair income redistribution and combating poverty constitutes some of
the fundamental tasks performed by countries all over the world.
Each country seeks a solution for these problems according to their
political, economic and administrative styles through applying
various economic and financial policies. The same situation can be
handled via “zakat” association in Islam. Nowadays, we observe
different versions of “zakat” in developed countries. Applications
such as negative income tax denote merely a different form of
“zakat” that is being applied almost in the same way but under
changed names. However, the minimum values to donate under zakat
(e.g. 85 gr. gold and 40 animals) get altered and various amounts are
put into practice. It might be named as negative income tax instead of
zakat, nonetheless, these applications are based on the Holy Koran
and the hadith released 1400 years ago. Besides, considering the
savage and slavery in the world at those times, we might easily
recognize the true value of the zakat being applied for the first time
then in the Islamic system. Through zakat, governments are able to
transfer incomes to the poor as a means of enabling them achieve the
minimum standard of living required. With regards to who benefits
from the Zakat, an objective and fair criteria was used to determine
who benefits from the zakat contrary to the notion that it was based
on peoples’ own choices. Since the zakat is obligatory, the transfers
do not get forwarded directly but via the government and get
distributed, which requires vast governmental organizations. Through
the application of Zakat, reduced levels of poverty can be achieved
and also ensure the fair income redistribution.
Abstract: The restrained construction zoning, an important part
in the urban master plan, is a necessary planning tool to control the city
sprawl, to guarantee the reservation implementation of the various
types of protective elements, and to realize the storage of the essential
urban spatial resources. Simultaneously, owing to the diverse
constitutes of restrained construction area and the various stakeholders
involved in, its planning requires an overall consideration of all
elements from the perspective of coordination+, balance and
practicability to deal with the problems and conflicts in this process.
Taking Yangzijin Ecological Restrained Construction Area in
Yangzhou as an example, this study analyzes all the potential actors,
agencies and stakeholders in this restrained construction area, as well
as the relevant conflicts between each other. Besides, this study tries to
build up a planning procedure based on the framework of governance
theory, and proposes a possible planning method that combines
"rigidity" and "flexibility" to protect the ecological limitation
boundary, to take every interest into account, and to promote economic
development in a harmonious society.
Abstract: Macro invertebrates have been used to monitor
organic pollution in rivers and streams. Several biotic indices based
on macro invertebrates have been developed over the years including
the Biological Monitoring Working Party (BMWP). A new biotic
index, the Gammarus:Asellus ratio has been recently proposed as an
index of organic pollution. This study tested the validity of the
Gammarus:Asellus ratio as an index of organic pollution, by
examining the relationship between the Gammarus:Asellus ratio and
physical chemical parameters, and other biotic indices such as
BMWP and, Average Score Per Taxon (ASPT) from lakes and
streams at Markeaton Park, Allestree Park and Kedleston Hall,
Derbyshire. Macro invertebrates were sampled using the standard
five minute kick sampling techniques physical and chemical
environmental variables were obtained based on standard sampling
techniques. Eighteen sites were sampled, six sites from Markeaton
Park (three sites across the stream and three sites across the lake). Six
sites each were also sampled from Allestree Park and Kedleston Hall
lakes. The Gammarus:Asellus ratio showed an opposite significant
positive correlations with parameters indicative of organic pollution
such as the level of nitrates, phosphates, and calcium and also
revealed a negatively significant correlations with other biotic indices
(BMWP/ASPT). The BMWP score correlated positively significantly
with some water quality parameters such as dissolved oxygen and
flow rate, but revealed no correlations with other chemical
environmental variables. The BMWP score was significantly higher
in the stream than the lake in Markeaton Park, also The ASPT scores
appear to be significantly higher in the upper Lakes than the middle
and lower lakes. This study has further strengthened the use of
BMWP/ASPT score as an index of organic pollution. But additional
application is required to validate the use of Gammarus:Asellus as a
rapid bio monitoring tool.
Abstract: In this investigation, we have evaluated the effects of
arsenic trioxide on hepatic function in pregnant and lactating Swiss
albino mice and their suckling pups. Experiments were carried out on
female mice given 175 ppm As2O3 in their drinking water from the
14th day of pregnancy until day 14 after delivery. Our results showed
a significant decrease in plasma levels of total protein and albumin,
cholesterol and triglyceride in As2O3 treated mice and their pups. The
hyperbilirubinemia and the increased plasma total alkaline
phosphatase activity suggested the presence of cholestasis.
Transaminase activities as well as lactate deshydrogenase activity in
plasma, known as biomarkers of hepatocellular injury, were elevated
indicating hepatic cells’ damage after treatment with As2O3.
Exposure to arsenic led to an increase of liver thiobarbituric acid
reactive substances level along with a concomitant decrease in the
activities of superoxide dismutase, catalase and glutathione
peroxidase and in glutathione.
Abstract: Although, arsenic trioxide has been the subject of
toxicological research, in vitro cytotoxicity and genotoxicity studies
using relevant cell models and uniform methodology are not well
elucidated. Hence, the aim of the present study was to evaluate the
cytotoxicity and genotoxicity induced by arsenic trioxide in human
keratinocytes (HaCaT) using the MTT [3-(4, 5-dimethylthiazol-2-yl)-
2,5-diphenyltetrazolium bromide] and alkaline single cell gel
electrophoresis (Comet) assays, respectively. Human keratinocytes
were treated with different doses of arsenic trioxide for 4 h prior to
cytogenetic assessment. Data obtained from the MTT assay indicated
that arsenic trioxide significantly reduced the viability of HaCaT cells
in a dose-dependent manner, showing an IC50 value of 34.18 ± 0.6
μM. Data generated from the comet assay also indicated a significant
dose-dependent increase in DNA damage in HaCaT cells associated
with arsenic trioxide exposure. We observed a significant increase in
comet tail length and tail moment, showing an evidence of arsenic
trioxide -induced genotoxic damage in HaCaT cells. This study
confirms that the comet assay is a sensitive and effective method to
detect DNA damage caused by arsenic.
Abstract: The concentration levels of six heavy metals (Cd, Cr,
Fe, Ni, Pb and Zn) and two mineral elements (Ca and Mg) were
determined in soil samples collected from the vicinity of two auto
mechanic workshops in Sabon-Gari, Kaduna state, Nigeria, using
Atomic Absorption Spectrometry (AAS), in order to compare the
gradation of their concentrations with distance and depth of soil from
the workshop sites. At site 1, concentrations of Lead, Chromium, Iron
and Zinc were generally found to be above the World Health
Organization limits, while those of Nickel and Cadmium fell within
the limits. Iron had the highest concentration with a range of 176.274
ppm to 489.127 ppm at depths of 5 cm to 15 cm and a distance range
of 5 m to 15 m, while the concentration of cadmium was least with a
range of 0.001 ppm to 0.008 ppm at similar depth and distance
ranges. In addition, there was more of calcium (11.521 ppm to
121.709 ppm), in all the samples, than magnesium (11.293 ppm to
21.635 ppm). Similar results were obtained for site II. The
concentrations of all the metals analyzed showed a downward
gradient with increase in depth and distance from both workshop sites
except for iron and zinc at site 2. The immediate and remote
implications of these findings on the biota are discussed.
Abstract: The study examined the effect of Bonny Light whole
crude oil (WC) and its water soluble fraction (WSF) on the activities
of antioxidant enzymes (catalase (CAT) and superoxide dismutase
(SOD)) and crude mitochondria ATPases in the radicle of
germinating bean (Vigna unguiculata). The percentage germination,
level of lipid peroxidation, antioxidant enzyme and mitochondria
Ca2+ and Mg2+ ATPase activities were measured in the radicle of
bean after 7, 14 and 21 days post germination. Viable bean seeds
were planted in soils contaminated with 10ml, 25ml and 50ml of
whole crude oil (WC) and its water soluble fraction (WSF) to obtain
2, 5 and 10% v/w crude oil contamination. There was dose dependent
reduction of the number of bean seeds that germinated in the
contaminated soils compared with control (p
Abstract: This study was conducted to investigate the effect of
the antioxidant activity of germinated African Yam Bean (AYB) on
oxidative stress markers in alloxan induced diabetic rat. Rats were
randomized into three groups; control, diabetic and germinated AYB
– treated diabetic rats. The Total phenol and flavonoid content and
DPPH radical scavenging activity before and after germination were
investigated. The glucose level, lipid peroxidation and reduced
glutathione of the animals were also determined using standard
technique for four weeks. Germination increased the total phenol,
flavonoid and antioxidant activity of AYB extract by 19.14%,
32.28% and 57.25% respectively. The diabetic rats placed on
germinated AYB diet had a significant decrease in the blood glucose
and lipid peroxidation with a corresponding increase in glutathione
(p
Abstract: Since columns are the most important elements of the
structures, failure of one column in a critical location can cause a
progressive collapse. In this respect, the repair and strengthening of
columns is a very important subject to reduce the building failure and
to keep the columns capacity. Twenty columns with different
parameters is tested and analysis. Eleven typical confined reinforced
concrete (RC) columns with different types of techniques are
assessment. And also, four confined concrete columns with plastic
tube (PVC) are tested with and with four paralleling tested of
unconfined plain concrete. The techniques of confined RC columns
are mortar strengthening, Steel rings strengthening, FRP
strengthening. Moreover, the technique of confined plain concrete
(PC) column is used PVC tubes. The columns are tested under
uniaxial compressive loads studied the effect of confinement on the
structural behavior of circular RC columns. Test results for each
column are presented in the form of crack patterns, stress-strain
curves. Test results show that confining of the RC columns using
different techniques of strengthening results significant improvement
of the general behavior of the columns and can used in construction.
And also, tested confined PC columns with PVC tubes results shown
that the confined PC with PVC tubes can be used in economical
building. The theoretical model for predicted column capacity is
founded with experimental factor depends on the confined techniques
used and the strain reduction.
Abstract: The use of information tools is a common activity for
students of any educational stage when they encounter online
learning activities. Finding the relevant information for particular
learning tasks is the topic of this paper as it investigates the use of
information tools for a group of student participants. The paper
describes and discusses the results with particular implications for
use in higher education, and the findings suggest that improvement in
assessment design and subsequent student learning may be achieved
by structuring the purposefulness of information tools usage and
online reading behaviors of university students.
Abstract: A knowledge-based expert system with the acronym
RASPE is developed as an application tool to help decision makers in
construction companies make informed decisions about managing
risks in pipeline construction projects. Choosing to use expert
systems from all available artificial intelligence techniques is due to
the fact that an expert system is more suited to representing a
domain’s knowledge and the reasoning behind domain-specific
decisions. The knowledge-based expert system can capture the
knowledge in the form of conditional rules which represent various
project scenarios and potential risk mitigation/response actions. The
built knowledge in RASPE is utilized through the underlying
inference engine that allows the firing of rules relevant to a project
scenario into consideration. Paper provides an overview of the
knowledge acquisition process and goes about describing the
knowledge structure which is divided up into four major modules.
The paper shows one module in full detail for illustration purposes
and concludes with insightful remarks.
Abstract: This research focused on comparing the critical
thinking of the teacher students before and after using Miller’s Model
learning activities and investigating their opinions. The sampling
groups were (1) fourth year 33 student teachers majoring in Early
Childhood Education and enrolling in semester 1 of academic year
2013 (2) third year 28 student teachers majoring in English and
enrolling in semester 2 of academic year 2013 and (3) third year 22
student teachers majoring in Thai and enrolling in semester 2 of
academic year 2013. The research instruments were (1) lesson plans
where the learning activities were settled based on Miller’s Model (2)
critical thinking assessment criteria and (3) a questionnaire on
opinions towards Miller’s Model based learning activities. The
statistical treatment was mean, deviation, different scores and T-test.
The result unfolded that (1) the critical thinking of the students after
the assigned activities was better than before and (2) the students’
opinions towards the critical thinking improvement activities based
on Miller’s Model ranged from the level of high to highest.
Abstract: One image is worth more than thousand words.
Images if analyzed can reveal useful information. Low level image
processing deals with the extraction of specific feature from a single
image. Now the question arises: What technique should be used to
extract patterns of very large and detailed image database? The
answer of the question is: “Image Mining”. Image Mining deals with
the extraction of image data relationship, implicit knowledge, and
another pattern from the collection of images or image database. It is
nothing but the extension of Data Mining. In the following paper, not
only we are going to scrutinize the current techniques of image
mining but also present a new technique for mining images using
Genetic Algorithm.
Abstract: The study of the electrical signals produced by neural
activities of human brain is called Electroencephalography. In this
paper, we propose an automatic and efficient EEG signal
classification approach. The proposed approach is used to classify the
EEG signal into two classes: epileptic seizure or not. In the proposed
approach, we start with extracting the features by applying Discrete
Wavelet Transform (DWT) in order to decompose the EEG signals
into sub-bands. These features, extracted from details and
approximation coefficients of DWT sub-bands, are used as input to
Principal Component Analysis (PCA). The classification is based on
reducing the feature dimension using PCA and deriving the supportvectors
using Support Vector Machine (SVM). The experimental are
performed on real and standard dataset. A very high level of
classification accuracy is obtained in the result of classification.
Abstract: The belief of a heavenly God is enshrined to all
Abrahamic religions which form the three major religions of the
world today. Muslims believe in Allah who is above the seven
heavens. The youth in the United Arab Emirates (UAE) study Islamic
courses as part of their high school curriculum and are required to
take at least one Islamic course at the university level to gain credit
hours towards their general education (GENED). This paper provides
an insight of what the youth studying in the UAE think of where
Allah is. Our analysis shows that a big number of Muslim youth were
not sure, especially those from the Middle Eastern and Arab countries
bringing to conclusion that this subject needs to be revisited again in
the course work.
Abstract: The well been of human beings on construction site is
very important, many man power had been lost through accidents
which kills or make workers physically unfit to carry out construction
activities, these in turn have multiple effects on the whole economy.
Thus it is necessary to put all safety items and regulations in place
before construction activities can commence. This study was carried
out in Ondo state of Nigeria to known and analyse the state of health
and safety of construction workers in the state. The study was done
using first hand observation method, 50 construction project sites
were visited in 10 major towns of Ondo state, questionnaires were
distributed and the results were analysed. The result show that
construction workers are being exposed to a lot of construction site
hazards due to lack of inadequate safety programmes and nonprovision
of appropriate safety materials for workers on site. From the
data gotten for each site visited and the statistical analysis, it can be
concluded that occurrence of accident on construction sites depends
significantly on the available safety facilities on the sites. The result of
the regression statistics show that the level of significant of the
dependence of occurrence of accident on the availability of safety
items on site is 0.0362 which is less than 0.05 maximum significant
level required. Therefore a vital way of sustaining our building
strategy is by given a detail attention to provision of adequate health
and safety items on construction sites which will reduce the
occurrence of accident, loss of man power and death of skilled
workers among others.