Antioxidant Enzymes and Crude Mitochondria ATPases in the Radicle of Germinating Bean (Vigna unguiculata) Exposed to Different Concentrations of Crude Oil

The study examined the effect of Bonny Light whole
crude oil (WC) and its water soluble fraction (WSF) on the activities
of antioxidant enzymes (catalase (CAT) and superoxide dismutase
(SOD)) and crude mitochondria ATPases in the radicle of
germinating bean (Vigna unguiculata). The percentage germination,
level of lipid peroxidation, antioxidant enzyme and mitochondria
Ca2+ and Mg2+ ATPase activities were measured in the radicle of
bean after 7, 14 and 21 days post germination. Viable bean seeds
were planted in soils contaminated with 10ml, 25ml and 50ml of
whole crude oil (WC) and its water soluble fraction (WSF) to obtain
2, 5 and 10% v/w crude oil contamination. There was dose dependent
reduction of the number of bean seeds that germinated in the
contaminated soils compared with control (p<0.001). The activities
of the antioxidant enzymes, as well as, adenosine triphosphatase
enzymes, were also significantly (p<0.001) altered in the radicle of
the plants grown in contaminated soil compared with the control.
Generally, the level of lipid peroxidation was highest after 21 days
post germination when compared with control. Stress to germinating
bean caused by Bonny Light crude oil or its water soluble fraction
resulted in adaptive changes in crude mitochondria ATPases in the
radicle.

• Stella O. Olubodun
• George E. Eriyamremu

• Antioxidant enzymes
• Bonny Light crude oil
• Radicle
• Mitochondria ATPases.

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