Abstract: Quantitative radiobiological models can be used to
assess the optimum clinical outcome from sophisticated therapeutic
modalities by calculating tumor control probability (TCP) and normal
tissue complication probability (NTCP). In this study two 3D-CRT
and an IMRT treatment plans were developed with an initial
prescription dose of 60 Gy in 2 Gy/fraction to prostate. Sensitivity of
TCP and Complication free tumor control probability (P+) to the
different values of α/β ratio was investigated for various prescription
doses planned to be delivered in either a fixed number of fractions (I)
or in a fixed dose per fraction (II) in each of the three different
treatment plans. High dose/fraction and high α/β value result in
comparatively smaller P+ and IMRT plans resulted in the highest P+,
mainly due to the decrease in NTCP. If α/β is lower than expected,
better tumor control can be achieved by increasing dose/fraction but
decreasing the number of fractions.
Abstract: Medicinal plants are now gaining attractiveness in
treatment of bacterial infections and food preservation. The objective
of this study was to assess antibacterial activity of some medicinal
plants on pathogenic bacteria. Screening of antibacterial activity of
aqueous and methanol extracts of some plants: Jojoba, Ginger, Sage,
Thyme and Clove against Bacillus cereus, Salmonella typhimurium,
Staphylococcus aureus, Clostridium perfringens and Escherichia coli
were investigated. Antibacterial activity was performed by agar
diffusion and disc diffusion method. Jatropha, Jojoba, Clove and
Ginger extracts showed notable bacterial activity in the first
screening step then selected to be tested against Bacillus cereus
(Gram+), Staphylococcus aureus (Gram+) and Salmonella
typhimurium (Gram−) and their effect was compared using
antibiotics as control. Screening results showed potential antibacterial
activity of the tested plant extracts against the screened bacterial
strains. It was found that methanol extracts exhibited higher
antibacterial activity than aqueous extracts. Methanol extract of
Jatropha showed the highest inhibition zone against Staphylococcus
aureus (Gram+) with 24.00 mm diameter, compared to the other
plant extracts followed by clove. Meanwhile, the inhibition zones of
methanol extracts of Jojoba and Ginger were the same (12mm).The
Gram-positive bacteria were found to be more sensitive to aqueous
and methanol extracts than Gram-negative bacteria.
Abstract: Durian is the flagship fruit of Mindanao and there is
an abundance of several cultivars with many confusing identities/
names.
The project was conducted to develop procedure for reliable and
rapid detection and sorting of durian planting materials. Moreover, it
is also aimed to establish specific genetic or DNA markers for routine
testing and authentication of durian cultivars in question.
The project developed molecular procedures for routine testing.
SSR primers were also screened and identified for their utility in
discriminating durian cultivars collected.
Results of the study showed the following accomplishments:
1. Twenty (29) SSR primers were selected and identified based on
their ability to discriminate durian cultivars,
2. Optimized and established standard procedure for identification
and authentication of Durian cultivars
3. Genetic profile of durian is now available at Biotech Unit
Our results demonstrate the relevance of using molecular
techniques in evaluating and identifying durian clones. The most
polymorphic primers tested in this study could be useful tools for
detecting variation even at the early stage of the plant especially for
commercial purposes. The process developed combines the efficiency
of the microsatellites development process with the optimization of
non-radioactive detection process resulting in a user-friendly protocol
that can be performed in two (2) weeks and easily incorporated into
laboratories about to start microsatellite development projects. This
can be of great importance to extend microsatellite analyses to other
crop species where minimal genetic information is currently
available. With this, the University can now be a service laboratory
for routine testing and authentication of durian clones.
Abstract: The grain quality of chickpea in Iran is low and
instable, which may be attributed to the evolution of cultivars with a
narrow genetic base making them vulnerable to biotic stresses. Four
chickpea varieties from diverse geographic origins were chosen and
arranged in a randomized complete block design. Mesorhizobium sp.
cicer strain SW7 was added to all the chickpea seeds. Chickpea seeds
were planted on October 9, 2013. Each genotype was sown 5 m in
length, with 35 cm inter-row spacing, in 3 rows. Weeds were
removed manually in all plots. Results showed that Analysis of
variance on the studied traits showed significant differences among
genotypes for N, P, K and Fe contents of chickpea, but there is not a
significant difference among Ca, Zn and Mg continents of chickpea.
The experimental coefficient of variation (CV) varied from 7.3 to
15.8. In general, the CV value lower than 20% is considered to be
good, indicating the accuracy of conducted experiments. The highest
grain N was observed in Hashem and Jam cultivars. The highest grain
P was observed in Jam cultivar. Phosphorus content (mg/100g)
ranged from 142.3 to 302.3 with a mean value of 221.3. The negative
correlation (-0.126) was observed between the N and P of chickpea
cultivars. The highest K and Fe contents were observed in Jam
cultivar.
Abstract: The efficiency of the actuation system of exoskeletons
and active orthoses for lower limbs is a significant aspect of the
design of such devices because it affects their efficacy. The F-IVT is
an innovative actuation system to power artificial knee joint with
energy recovery capabilities. Its key and non-conventional elements
are a flywheel that acts as a mechanical energy storage system, and
an Infinitely Variable Transmission (IVT). The design of the F-IVT
can be optimized for a certain walking condition, resulting in a heavy
reduction of both the electric energy consumption and of the electric
peak power. In this work, by means of simulations of level ground
walking at different speeds, it is demonstrated that the F-IVT is still
an advantageous actuator which permits to save energy consumption
and to downsize the electric motor even when it does not work in
nominal conditions.
Abstract: A chromium-loaded ash originating from incineration of tannery sludge under anoxic conditions was mixed with low grade soda-lime glass powder coming from commercial glass bottles. The relative weight proportions of ash over glass powder tested were 30/70, 40/60 and 50/50. The solid mixtures, formed in green state compacts, were sintered at the temperature range of 800o C up to 1200o C. The resulting products were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectrometry (EDXS) and micro-indentation. The above methods were employed to characterize the various phases, microstructure and hardness of the produced materials. Thermal treatment at 800o C and 1000o C produced opaque ceramic products composed of a variety of chromium-containing and chromium-free crystalline phases. Thermal treatment at 1200o C gave rise to composite products, where only chromium-containing crystalline phases were detected. Hardness results suggest that specific products are serious candidates for structural applications.
Abstract: Moving into a new era of healthcare, new tools and
devices are developed to extend and improve health services, such as
remote patient monitoring and risk prevention. In this concept,
Internet of Things (IoT) and Cloud Computing present great
advantages by providing remote and efficient services, as well as
cooperation between patients, clinicians, researchers and other health
professionals. This paper focuses on patients suffering from bipolar
disorder, a brain disorder that belongs to a group of conditions
called affective disorders, which is characterized by great mood
swings. We exploit the advantages of Semantic Web and Cloud
Technologies to develop a patient monitoring system to support
clinicians. Based on intelligently filtering of evidence-knowledge and
individual-specific information we aim to provide treatment
notifications and recommended function tests at appropriate times or
concluding into alerts for serious mood changes and patient’s nonresponse
to treatment. We propose an architecture as the back-end
part of a cloud platform for IoT, intertwining intelligence devices
with patients’ daily routine and clinicians’ support.
Abstract: In this article, the antibiogram and heavy metal
resistance profile of the bacteria isolated from total 34 studied
animals (Pelophylax ridibundus = 12; Mauremys rivulata = 14;
Natrix natrix = 8) captured around the Biga Stream, are described.
There was no database information on antibiogram and heavy metal
resistance profile of bacteria from these area’s amphibians and
reptiles.
A total of 200 bacteria were successfully isolated from cloaca and
oral samples of the aquatic amphibians and reptiles as well as from
the water sample. According to Jaccard’s similarity index, the degree
of similarity in the bacterial flora was quite high among the
amphibian and reptile species under examination, whereas it was
different from the bacterial diversity in the water sample. The most
frequent isolates were A. hydrophila (31.5%), B. pseudomallei
(8.5%), and C. freundii (7%). The total numbers of bacteria obtained
were as follows: 45 in P. ridibundus, 45 in N. natrix 30 in M.
rivulata, and 80 in the water sample. The result showed that
cefmetazole was the most effective antibiotic to control the bacteria
isolated in this study and that approximately 93.33% of the bacterial
isolates were sensitive to this antibiotic. The multiple antibiotic
resistances (MAR) index indicated that P. ridibundus (0.95) > N.
natrix (0.89) > M. rivulata (0.39). Furthermore, all the tested heavy
metals (Pb+2, Cu+2, Cr+3, and Mn+2) inhibit the growth of the bacterial
isolates at different rates. Therefore, it indicated that the water source
of the animals was contaminated with both antibiotic residues and
heavy metals.
Abstract: The aim of this paper is to present the optimization
methodology developed in the frame of a Coastal Transport
Information System. The system will be used for the effective design
of coastal transportation lines and incorporates subsystems that
implement models, tools and techniques that may support the design
of improved networks. The role of the optimization and decision
subsystem is to provide the user with better and optimal scenarios
that will best fulfill any constrains, goals or requirements posed. The
complexity of the problem and the large number of parameters and
objectives involved led to the adoption of an evolutionary method
(Genetic Algorithms). The problem model and the subsystem
structure are presented in detail, and, its support for simulation is also
discussed.
Abstract: Freshly laid eggs from green turtles, Chelonia mydas,
were randomly collected from Ras Al-Hadd Reserve, Oman.
Eggshells taken from eggs and sand collected from the body chamber
were analyzed for eight heavy metals (Al, Br, Cd, Co, Cu, Fe, S, and
Zn) using inductively coupled plasma mass spectrometry (ICP).
Heavy metal concentrations varied significantly (P
Abstract: Essential oils have a significant antimicrobial activity.
These oils can successfully replace the antibiotics. So, the
microorganisms show their inefficiencies resistant for the antibiotics.
For this reason, we study the physicochemical analysis and
antimicrobial activity of the essential oil of Daucus carota. The
extraction is done by steam distillation of water which brought us a
very significant return of 4.65%. The analysis of the essential oil is
performed by GC / MS and has allowed us to identify 32 compounds
in the oil of D. carota flowering tops of Bouira. Three of which are in
the majority are the α-Pinene (22.3%), the carotol (21.7%) and the
limonene (15.8%).
Abstract: The Malaysian government had consistently revived
its campaign for “Buy Malaysian Goods” from time to time. The
purpose of the campaign is to remind consumers to be ethnocentric
and patriotic when purchasing product and services. This is necessary
to ensure high demand for local products and services compared to
foreign products. However, the decline of domestic investment in
2012 has triggered concern for the Malaysian economy. Hence, this
study attempts to determine the drivers of actual purchasing behavior,
intention to purchase domestic products and ethnocentrism. The
study employs the cross-sectional primary data, self-administered on
household, selected using stratified random sampling in four
Malaysian regions. A nine factor driver of actual domestic purchasing
behavior (culture openness, conservatism, collectivism, patriotism,
control belief, interest in foreign travel, attitude, ethnocentrism and
intention) were measured utilizing 60 items, using 7-point Likertscale.
From 1000 questionnaires distributed, a sample of 486 were
returned representing 48.6 percent response rate. From the fit
generated structural model (SEM analysis), it was found that the
drivers of actual purchase behavior are collectivism, cultural
openness and patriotism; the drivers of intention to purchase
domestic product are attitude, control belief, collectivism and
conservatism; and drivers of ethnocentrism are cultural openness,
control belief, foreign travel and patriotism. It also shows that
Malaysian consumers scored high in ethnocentrism and patriotism.
The findings are discussed in the perspective of its implication to
Malaysian National Agenda.
Abstract: Enterococci are important inhabitants of the animal
intestine and are widely used in probiotic products. A probiotic strain
is expected to possess several desirable properties in order to exert
beneficial effects. Therefore, the objective of this study was to
isolate, characterize and identify Enterococcus sp. from chicken cecal
and fecal samples to determine potential probiotic properties.
Enterococci were isolated from chicken ceca and feces of thirty three
clinically healthy chickens from a local farm. In vitro studies were
performed to assess antibacterial activity of the isolated LAB (using
agar well diffusion and cell free supernatant broth technique against
Salmonella enterica serotype Enteritidis), survival in acidic
conditions, resistance to bile salts, and their survival during simulated
gastric juice conditions at pH 2.5. Isolates were identified by
biochemical carbohydrate fermentation patterns using an API 50
CHL kit and API ZYM kits and by sequenced 16S rDNA. An isolate
belonging to E. faecium species exhibited inhibitory effect against S.
enteritidis. This isolate producing a clear zone as large as 10.30 mm
or greater and was able to grow in the coculture medium and at the
same time, inhibited the growth S. enteritidis. In addition, E. faecium
exhibited significant resistance under highly acidic conditions at pH
2.5 for 8 h and survived well in bile salt at 0.2% for 24 h and showing
ability to survive in the presence of simulated gastric juice at pH 2.5.
Based on these results, E. faecium isolate fulfills some of the criteria
to be considered as a probiotic strain and therefore, could be used as a
feed additive with good potential for controlling S. Enteritidis in
chickens. However, in vivo studies are needed to determine the safety
of the strain.
Abstract: The management of Helicobacter pylori (H. pylori)
eradication is still a matter of discussion, full effectiveness is rarely
achieved, and it has many adverse effects. The use of probiotics may
be associated with better eradication rates and possibly prevention of
adverse events due to antibiotic therapy. The present clinical study
was undertaken to evaluate the efficacy of a specially designed
fermented milk product, containing Bifidobacterium lactis B420, on
the eradication of H. pylori infection in a prospective, randomized,
double-blind, controlled study in humans. Four test fermented milks
(FM) were specially designed in which counts of viable cells in all
products were 10^10 Log CFU. 100 mL-1 for Bifidobacterium lactis -
Bifidobacterium species 420. 190 subjects infected with H. pylori,
with previous diagnosis of functional dyspepsia according to Rome
III criteria entered the study. Bifidobacterium lactis B420,
administered twice a day for 90 days was not able to eradicate H.
pylori in Brazilian patients with functional dyspepsia.
Abstract: Agriculture is the backbone of economy of Pakistan
and cotton is the major agricultural export and supreme source of raw
fiber for our textile industry. To combat severe problems of insect
and weed, combination of three genes namely Cry1Ac, Cry2A and
EPSPS genes was transferred in locally cultivated cotton variety
MNH-786 with the use of Agrobacterium mediated genetic
transformation. The present study focused on the molecular screening
of transgenic cotton plants at T3 generation in order to confirm
integration and expression of all three genes (Cry1Ac, Cry2A and
EPSP synthase) into the cotton genome. Initially, glyphosate spray
assay was used for screening of transgenic cotton plants containing
EPSP synthase gene at T3 generation. Transgenic cotton plants which
were healthy and showed no damage on leaves were selected after 07
days of spray. For molecular analysis of transgenic cotton plants in
the laboratory, the genomic DNA of these transgenic cotton plants
were isolated and subjected to amplification of the three genes. Thus,
seventeen out of twenty (Cry1Ac gene), ten out of twenty (Cry2A
gene) and all twenty (EPSP synthase gene) were produced positive
amplification. On the base of PCR amplification, ten transgenic plant
samples were subjected to protein expression analysis through
ELISA. The results showed that eight out of ten plants were actively
expressing the three transgenes. Real-time PCR was also done to
quantify the mRNA expression levels of Cry1Ac and EPSP synthase
gene. Finally, eight plants were confirmed for the presence and active
expression of all three genes at T3 generation.
Abstract: Macro invertebrates have been used to monitor
organic pollution in rivers and streams. Several biotic indices based
on macro invertebrates have been developed over the years including
the Biological Monitoring Working Party (BMWP). A new biotic
index, the Gammarus:Asellus ratio has been recently proposed as an
index of organic pollution. This study tested the validity of the
Gammarus:Asellus ratio as an index of organic pollution, by
examining the relationship between the Gammarus:Asellus ratio and
physical chemical parameters, and other biotic indices such as
BMWP and, Average Score Per Taxon (ASPT) from lakes and
streams at Markeaton Park, Allestree Park and Kedleston Hall,
Derbyshire. Macro invertebrates were sampled using the standard
five minute kick sampling techniques physical and chemical
environmental variables were obtained based on standard sampling
techniques. Eighteen sites were sampled, six sites from Markeaton
Park (three sites across the stream and three sites across the lake). Six
sites each were also sampled from Allestree Park and Kedleston Hall
lakes. The Gammarus:Asellus ratio showed an opposite significant
positive correlations with parameters indicative of organic pollution
such as the level of nitrates, phosphates, and calcium and also
revealed a negatively significant correlations with other biotic indices
(BMWP/ASPT). The BMWP score correlated positively significantly
with some water quality parameters such as dissolved oxygen and
flow rate, but revealed no correlations with other chemical
environmental variables. The BMWP score was significantly higher
in the stream than the lake in Markeaton Park, also The ASPT scores
appear to be significantly higher in the upper Lakes than the middle
and lower lakes. This study has further strengthened the use of
BMWP/ASPT score as an index of organic pollution. But additional
application is required to validate the use of Gammarus:Asellus as a
rapid bio monitoring tool.
Abstract: The concentration levels of six heavy metals (Cd, Cr,
Fe, Ni, Pb and Zn) and two mineral elements (Ca and Mg) were
determined in soil samples collected from the vicinity of two auto
mechanic workshops in Sabon-Gari, Kaduna state, Nigeria, using
Atomic Absorption Spectrometry (AAS), in order to compare the
gradation of their concentrations with distance and depth of soil from
the workshop sites. At site 1, concentrations of Lead, Chromium, Iron
and Zinc were generally found to be above the World Health
Organization limits, while those of Nickel and Cadmium fell within
the limits. Iron had the highest concentration with a range of 176.274
ppm to 489.127 ppm at depths of 5 cm to 15 cm and a distance range
of 5 m to 15 m, while the concentration of cadmium was least with a
range of 0.001 ppm to 0.008 ppm at similar depth and distance
ranges. In addition, there was more of calcium (11.521 ppm to
121.709 ppm), in all the samples, than magnesium (11.293 ppm to
21.635 ppm). Similar results were obtained for site II. The
concentrations of all the metals analyzed showed a downward
gradient with increase in depth and distance from both workshop sites
except for iron and zinc at site 2. The immediate and remote
implications of these findings on the biota are discussed.
Abstract: The present paper summarizes the analysis of the
request for consultation of information and data on industrial
emissions made publicly available on the web site of the Ministry of
Environment, Land and Sea on integrated pollution prevention and
control from large industrial installations, the so called “AIA Portal”.
As a matter of fact, a huge amount of information on national
industrial plants is already available on internet, although it is usually
proposed as textual documentation or images.
Thus, it is not possible to access all the relevant information
through interoperability systems and also to retrieval relevant
information for decision making purposes as well as rising of
awareness on environmental issue.
Moreover, since in Italy the number of institutional and private
subjects involved in the management of the public information on
industrial emissions is substantial, the access to the information is
provided on internet web sites according to different criteria; thus, at
present it is not structurally homogeneous and comparable.
To overcome the mentioned difficulties in the case of the
Coordinating Committee for the implementation of the Agreement
for the industrial area in Taranto and Statte, operating before the
IPPC permit granting procedures of the relevant installation located
in the area, a big effort was devoted to elaborate and to validate data
and information on characterization of soil, ground water aquifer and
coastal sea at disposal of different subjects to derive a global
perspective for decision making purposes. Thus, the present paper
also focuses on main outcomes matured during such experience.
Abstract: Advanced head and neck cancers are aggressive
tumours, which require aggressive treatment. Treatment efficiency is
often hindered by cancer cell repopulation during radiotherapy,
which is due to various mechanisms triggered by the loss of tumour
cells and involves both stem and differentiated cells. The aim of the
current paper is to present in silico simulations of radiotherapy
schedules on a virtual head and neck tumour grown with biologically
realistic kinetic parameters. Using the linear quadratic formalism of
cell survival after radiotherapy, altered fractionation schedules
employing various treatment breaks for normal tissue recovery are
simulated and repopulation mechanism implemented in order to
evaluate the impact of various cancer cell contribution on tumour
behaviour during irradiation. The model has shown that the timing of
treatment breaks is an important factor influencing tumour control in
rapidly proliferating tissues such as squamous cell carcinomas of the
head and neck. Furthermore, not only stem cells but also
differentiated cells, via the mechanism of abortive division, can
contribute to malignant cell repopulation during treatment.
Abstract: Member States shall establish zones and
agglomerations throughout their territory to assess and manage air
quality in order to comply with European directives.
In Italy decree 155/2010, transposing Directive 2008/50/EC on
ambient air quality and cleaner air for Europe, merged into a single
act the previous provisions on ambient air quality assessment and
management, including those resulting from the implementation of
Directive 2004/107/EC relating to arsenic, cadmium, nickel, mercury
and polycyclic aromatic hydrocarbons in ambient air.
Decree 155/2010 introduced stricter rules for identifying zones on
the basis of the characteristics of the territory in spite of considering
pollution levels, as it was in the past. The implementation of such
new criteria has reduced the great variability of the previous zoning,
leading to a significant reduction of the total number of zones and to
a complete and uniform ambient air quality assessment and
management throughout the Country.
The present document is related to the new zones definition in
Italy according to Decree 155/2010. In particular the paper contains
the description and the analysis of the outcome of zoning and
classification.