Abstract: More than 3000 plants of notable phyto-therapeutic
value grow in South Africa; these include Cissampelos capensis,
commonly known in Afrikaans as dawidjie or dawidjiewortel. C.
capensis is the most significant and popular medicinal plant used by
the Khoisan as well as other rural groups in the Western region of
South Africa. Its rhizomes are traditionally used to treat male fertility
problems. Yet, no studies have investigated the effects of this plant or
its extracts on human spermatozoa. Therefore, this study aimed at
investigating the effects of C. capensis rhizome extract (CRE)
fractions on ejaculated human spermatozoa in vitro. Spermatozoa
from a total of 77 semen samples were washed with human tubular
fluid medium supplemented with bovine serum albumin (HTF-BSA)
and incubated for 2 hours with 20 μg/ml progesterone (P4) followed
by incubation with different concentrations (0, 0.05, 0.5, 5, 50, 200
μg/ml) of fractionated CRE (F1=0% MeOH, F2=30% MeOH,
F3=60% MeOH and F4=100% MeOH) for 1.5 hours at 37°C. A
sample without addition of CRE fractions served as control. Samples
were analyzed for sperm motility, reactive oxygen species (ROS),
DNA-fragmentation, acrosome reaction and capacitation. Results
showed that F1 resulted in significantly higher values for ROS,
capacitation and hyper-activation compared to F2, F3, and F4 with
P4-stimulated samples generally having higher values. No significant
effect was found for the other parameters. In conclusion, alkaloids
present in F1 of CRE appear to have triggered sperm intrinsic ROS
production leading to sperm capacitation and acrosome reaction
induced by P4.
Abstract: It is the worldwide problem that the recycled PVB is
not recycled and it is wildly stored in landfills. However, PVB has
similar chemical properties such as PVC. Moreover, both of these
polymers are plasticized. Therefore, the study of thermal properties
of plasticized PVC and the recycled PVB obtained by recycling of
windshields is carried out. This work has done in order to find nondegradable
processing conditions applicable for both polymers.
Tested PVC contained 38% of plasticizer diisononyl phthalate
(DINP) and PVB was plasticized with 28% of triethylene glycol,
bis(2-ethylhexanoate) (3GO). The thermal and thermo-oxidative
decomposition of both vinyl polymers are compared by calorimetric
analysis and by tensile strength analysis.
Abstract: Steady three-dimensional and two free surface waves
generated by moving bodies are presented, the flow problem to be
simulated is rich in complexity and poses many modeling challenges
because of the existence of breaking waves around the ship hull, and
because of the interaction of the two-phase flow with the turbulent
boundary layer. The results of several simulations are reported. The
first study was performed for NACA0012 of hydrofoil with different
meshes, this section is analyzed at h/c= 1, 0345 for 2D. In the second
simulation a mathematically defined Wigley hull form is used to
investigate the application of a commercial CFD code in prediction of
the total resistance and its components from tangential and normal
forces on the hull wetted surface. The computed resistance and wave
profiles are used to estimate the coefficient of the total resistance for
Wigley hull advancing in calm water under steady conditions. The
commercial CFD software FLUENT version 12 is used for the
computations in the present study. The calculated grid is established
using the code computer GAMBIT 2.3.26. The shear stress k-ωSST
model is used for turbulence modeling and the volume of fluid
technique is employed to simulate the free-surface motion. The
second order upwind scheme is used for discretizing the convection
terms in the momentum transport equations, the Modified HRIC
scheme for VOF discretization. The results obtained compare well
with the experimental data.
Abstract: Image segmentation and color identification is an
important process used in various emerging fields like intelligent
robotics. A method is proposed for the manipulator to grasp and place
the color object into correct location. The existing methods such as
PSO, has problems like accelerating the convergence speed and
converging to a local minimum leading to sub optimal performance.
To improve the performance, we are using watershed algorithm and
for color identification, we are using EPSO. EPSO method is used to
reduce the probability of being stuck in the local minimum. The
proposed method offers the particles a more powerful global
exploration capability. EPSO methods can determine the particles
stuck in the local minimum and can also enhance learning speed as
the particle movement will be faster.
Abstract: Whey is the lactose rich by-product of the dairy
industry, having good amount of nutrient reservoir. Most abundant
nutrients are lactose, soluble proteins, lipids and mineral salts.
Disposing of whey by most of milk plants which do not have proper
pre-treatment system is the major issue. As a result of which, there
can be significant loss of potential food and energy source. Thus,
whey has been explored as the substrate for the synthesis of different
value added products such as enzymes. β-galactosidase is one of the
important enzymes and has become the major focus of research due
to its ability to catalyze both hydrolytic as well as
transgalactosylation reaction simultaneously. The enzyme is widely
used in dairy industry as it catalyzes the transformation of lactose to
glucose and galactose, making it suitable for the lactose intolerant
people. The enzyme is intracellular in both bacteria and yeast,
whereas for molds, it has an extracellular location. The present work
was carried to utilize the whey for the production of β-galactosidase
enzyme using both yeast and fungal cultures. The yeast isolate
Kluyveromyces marxianus WIG2 and various fungal strains have
been used in the present study. Different disruption techniques have
also been investigated for the extraction of the enzyme produced
intracellularly from yeast cells. Among the different methods tested
for the disruption of yeast cells, SDS-chloroform showed the
maximum β-galactosidase activity. In case of the tested fungal
cultures, Aureobasidium pullulans NCIM 1050 was observed to be
the maximum extracellular enzyme producer.
Abstract: Nowadays, the rapid development of CAD systems’
programming environments results in the creation of multiple
downstream applications, which are developed and becoming
increasingly available. CAD based manufacturing simulations is
gradually following the same trend. Drilling is the most popular holemaking
process used in a variety of industries. A specially built piece
of software that deals with the drilling kinematics is presented. The
cutting forces are calculated based on the tool geometry, the cutting
conditions and the tool/work-piece materials. The results are verified
by experimental work. Finally, the response surface methodology
(RSM) is applied and mathematical models of the total thrust force
and the thrust force developed because of the main cutting edges are
proposed.
Abstract: Metal thin-walled members have been widely used in
building industry. Usually they are utilized as purlins, girts or ceiling
beams. Due to slenderness of thin-walled cross-sections these
structural members are prone to stability problems (e.g. flexural
buckling, lateral torsional buckling). If buckling is not
constructionally prevented their resistance is limited by buckling
strength. In practice planar members of roof or wall cladding can be
attached to thin-walled members. These elements reduce
displacement of thin-walled members and therefore increase their
buckling strength. If this effect is taken into static assessment more
economical sections of thin-walled members might be utilized and
certain savings of material might be achieved. This paper focuses on
problem of determination of critical load of steel thin-walled beams
with lateral continuous restraint which is crucial for lateral torsional
buckling assessment.
Abstract: Aluminium matrix composites with alumina
reinforcements give superior mechanical & physical properties. Their
applications in several fields like automobile, aerospace, defense,
sports, electronics, bio-medical and other industrial purposes are
becoming essential for the last several decades. In the present work,
fabrication of hybrid composite was done by Stir casting technique
using Al 6061 as a matrix with alumina and silicon carbide (SiC) as
reinforcement materials. The weight percentage of alumina is varied
from 2 to 4% and the silicon carbide weight percentage is maintained
constant at 2%. Hardness and wear tests are performed in the as cast
and heat treated conditions. Age hardening treatment was performed
on the specimen with solutionizing at 550°C, aging at two
temperatures (150 and 200°C) for different time durations. Hardness
distribution curves are drawn and peak hardness values are recorded.
Hardness increase was very sensitive with respect to the decrease in
aging temperature. There was an improvement in wear resistance of
the peak aged material when aged at lower temperature. Also
increase in weight percent of alumina, increases wear resistance at
lower temperature but opposite behavior was seen when aged at
higher temperature.
Abstract: This paper addresses a cutting edge method of
business demand forecasting, based on an empirical probability
function when the historical behavior of the data is random.
Additionally, it presents error determination based on the numerical
method technique ‘propagation of errors.’ The methodology was
conducted characterization and process diagnostics demand planning
as part of the production management, then new ways to predict its
value through techniques of probability and to calculate their mistake
investigated, it was tools used numerical methods. All this based on
the behavior of the data. This analysis was determined considering
the specific business circumstances of a company in the sector of
communications, located in the city of Bogota, Colombia. In
conclusion, using this application it was possible to obtain the
adequate stock of the products required by the company to provide its
services, helping the company reduce its service time, increase the
client satisfaction rate, reduce stock which has not been in rotation
for a long time, code its inventory, and plan reorder points for the
replenishment of stock.
Abstract: The present study was aimed to examine the structure
of children’s adaptation during school transition and to identify a
commonality and dissimilarity at the elementary and junior high
school. 1,983 students in the 6th grade and 2,051 students in the 7th
grade were extracted by stratified two-stage random sampling and
completed the ASSESS that evaluated the school adaptation from the
view point of ‘general satisfaction’, ‘teachers’ support’, ‘friends’
support’, ‘anti-bullying relationship’, ‘prosocial skills’, and ‘academic
adaptation’. The 7th graders tend to be worse adaptation than the 6th
graders. A structural equation modeling showed the goodness of fit for
each grades. Both models were very similar but the 7th graders’ model
showed a lower coefficient at the pass from ‘teachers’ support’ to
‘friends’ support’. The role of ‘teachers’ support’ was decreased to
keep a good relation in junior high school. We also discussed how we
provide a continuous assistance for prevention of the 7th graders’ gap.
Abstract: In order to address construction project requirements
and specifications, scholars and practitioners need to establish
taxonomy according to a scheme that best fits their need. While
existing characterization methods are continuously being improved,
new ones are devised to cover project properties which have not been
previously addressed. One such method, the Project Definition Rating
Index (PDRI), has received limited consideration strictly as a
classification scheme. Developed by the Construction Industry
Institute (CII) in 1996, the PDRI has been refined over the last two
decades as a method for evaluating a project's scope definition
completeness during front-end planning (FEP). The main
contribution of this study is a review of practical project classification
methods, and a discussion of how PDRI can be used to classify
projects based on their readiness in the FEP phase. The proposed
model has been applied to 59 construction projects in Ontario, and
the results are discussed.
Abstract: Methicillin/multiple-resistant Staphylococcus aureus
(MRSA) are infectious bacteria that are resistant to common
antibiotics. A previous in silico study in our group has identified a
hypothetical protein SAV1226 as one of the potential drug targets. In
this study, we reported the bioinformatics characterization, as well as
cloning, expression, purification and kinetic assays of hypothetical
protein SAV1226 from methicillin/vancomycin-resistant
Staphylococcus aureus Mu50 strain. MALDI-TOF/MS analysis
revealed a low degree of structural similarity with known proteins.
Kinetic assays demonstrated that hypothetical protein SAV1226 is
neither a domain of an ATP dependent dihydroxyacetone kinase nor
of a phosphotransferase system (PTS) dihydroxyacetone kinase,
suggesting that the function of hypothetical protein SAV1226 might
be misannotated on public databases such as UniProt and
InterProScan 5.
Abstract: This study examines the feasibility of indirect solar
desalination in oil producing countries in the Middle East and North
Africa (MENA) region. It relies on value engineering (VE) and costbenefit
with sensitivity analyses to identify optimal coupling
configurations of desalination and solar energy technologies. A
comparative return on investment was assessed as a function of water
costs for varied plant capacities (25,000 to 75,000 m3/day), project
lifetimes (15 to 25 years), and discount rates (5 to 15%) taking into
consideration water and energy subsidies, land cost as well as
environmental externalities in the form of carbon credit related to
greenhouse gas (GHG) emissions reduction. The results showed
reverse osmosis (RO) coupled with photovoltaic technologies (PVs)
as the most promising configuration, robust across different prices for
Brent oil, discount rates, as well as different project lifetimes.
Environmental externalities and subsidies analysis revealed that a
16% reduction in existing subsidy on water tariffs would ensure
economic viability. Additionally, while land costs affect investment
attractiveness, the viability of RO coupled with PV remains possible
for a land purchase cost
Abstract: When neck pain is associated with pain, numbness, or
weakness in the arm, shoulder, or hand, further investigation is
needed as these are symptoms indicating pressure on one or more
nerve roots. Evaluation necessitates a neurologic examination and
imaging using an MRI/CT scan. A degenerating disc loses some
thickness and is less flexible, causing inter-vertebrae space to narrow.
A radiologist diagnoses an Intervertebral Disc Degeneration (IDD) by
localizing every inter-vertebral disc and identifying the pathology in
a disc based on its geometry and appearance. Accurate localizing is
necessary to diagnose IDD pathology. But, the underlying image
signal is ambiguous: a disc’s intensity overlaps the spinal nerve
fibres. Even the structure changes from case to case, with possible
spinal column bending (scoliosis). The inter-vertebral disc
pathology’s quantitative assessment needs accurate localization of the
cervical region discs. In this work, the efficacy of multilevel set
segmentation model, to segment cervical discs is investigated. The
segmented images are annotated using a simple distance matrix.
Abstract: Bringing forth a survey on recent higher order spline
techniques for solving boundary value problems in ordinary
differential equations. Here we have discussed the summary of the
articles since 2000 till date based on higher order splines like Septic,
Octic, Nonic, Tenth, Eleventh, Twelfth and Thirteenth Degree
splines. Comparisons of methods with own critical comments as
remarks have been included.
Abstract: Steady three-dimensional and two free surface waves
generated by moving bodies are presented, the flow problem to be
simulated is rich in complexity and poses many modeling challenges
because of the existence of breaking waves around the ship hull, and
because of the interaction of the two-phase flow with the turbulent
boundary layer. The results of several simulations are reported. The
first study was performed for NACA0012 of hydrofoil with different
meshes, this section is analyzed at h/c= 1, 0345 for 2D. In the second
simulation a mathematically defined Wigley hull form is used to
investigate the application of a commercial CFD code in prediction of
the total resistance and its components from tangential and normal
forces on the hull wetted surface. The computed resistance and wave
profiles are used to estimate the coefficient of the total resistance for
Wigley hull advancing in calm water under steady conditions. The
commercial CFD software FLUENT version 12 is used for the
computations in the present study. The calculated grid is established
using the code computer GAMBIT 2.3.26. The shear stress k-ωSST
model is used for turbulence modeling and the volume of fluid
technique is employed to simulate the free-surface motion. The
second order upwind scheme is used for discretizing the convection
terms in the momentum transport equations, the Modified HRIC
scheme for VOF discretization. The results obtained compare well
with the experimental data.
Abstract: A compound parabolic concentrator (CPC) is a wellknown
non-imaging concentrator that will concentrate the solar
radiation onto receiver (PV cell). One of disadvantage of CPC is has
tall and narrow height compared to its diameter entry aperture area.
Therefore, for economic reason, a truncation had been done by
removed from the top of the full height CPC. This also will lead to
the decreases of concentration ratio but it will be negligible. In this
paper, the flux distribution of untruncated and truncated 2-D hollow
compound parabolic trough concentrator (hCPTC) design is
presented. The untruncated design has initial height H=193.4mm
with concentration ratio C_(2-D)=4. This paper presents the optical
simulation of compound parabolic trough concentrator using raytracing
software TracePro. Results showed that, after the truncation,
the height of CPC reduced 45% from initial height with the
geometrical concentration ratio only decrease 10%. Thus, the cost of
reflector and material dielectric usage can be saved especially at
manufacturing site.
Abstract: Nowadays, the main goal for modern horticultural
production is an increase the quality. In recent years, the use of
organic fertilizers or biostimulants that can be applied in agriculture
to improve quali-quantitative crop yields has encountered increasing
interest. Biostimulants are gaining importance also for their possible
use in organic and sustainable agriculture, to avoid excessive
fertilizer applications. Consecutive experimental trials were carried
out in the Apulia region (southern Italy) on three herbaceous crops
(cauliflower, pepper, fennel) grown in pots under conventional and
organic fertilization systems without and with biostimulants. The aim
was to determine the effects of three biostimulants (Siapton®10L,
Micotech L, Lysodin Alga-Fert) on quali-quantitative yield
characteristics. At harvest, the quali-quantitative yield characteristics
of each crop were determined. All of the experimental data were
subjected to analysis of variance (ANOVA), and when significant
effects were detected, the means were compared using Tukey’s tests.
These data show large differences in these yield characteristics
between conventional and organic crops, particularly highlighting
higher yields for the conventional crops, while variable results were
generally observed when the biostimulants were applied. In this
context, there were no effects of the biostimulants on the quantitative
yield, whereas there were low positive effects on the qualitative
characteristics, as related to higher dry matter content of cauliflower,
and higher soluble solids content of pepper. Moreover, there were
evident positive effects of the biostimulants with fennel, due to the
lower nitrate content. These latter data are in line with most of the
published literature obtained for other herbaceous crops.
Abstract: To ensure targeting of apoferritin nanocarrier with
encapsulated doxorubicin drug, we used a peptide linker based on a
protein G with N-terminus affinity towards Fc region of antibodies.
To connect the peptide to the surface of apoferritin, the C-terminus of
peptide was made of cysteine with affinity to gold. The surface of
apoferritin with encapsulated doxorubicin (APODOX) was coated
either with gold nanoparticles (APODOX-Nano) or gold(III) chloride
hydrate reduced with sodium borohydride (APODOX-HAu). The
reduction with sodium borohydride caused a loss of doxorubicin
fluorescent properties and probably accompanied with the loss of its
biological activity. Fluorescent properties of APODOX-Nano were
similar to the unmodified APODOX; therefore it was more suited for
the intended use. To evaluate the specificity of apoferritin modified
with antibodies, ELISA-like method was used with the surface of
microtitration plate wells coated by the antigen (goat anti-human IgG
antibodies). To these wells, the nanocarrier was applied. APODOX
without the modification showed 5× lower affinity to the antigen than
APODOX-Nano modified gold and targeting antibodies (human IgG
antibodies).
Abstract: The novel 3D SnO cabbages self-assembled by
nanosheets were successfully synthesized via template-free
hydrothermal growth method under facile conditions. The XRD
results manifest that the as-prepared SnO is tetragonal phase. The
TEM and HRTEM results show that the cabbage nanosheets are
polycrystalline structure consisted of considerable single-crystalline
nanoparticles. Two typical Raman modes A1g=210 and Eg=112 cm-1
of SnO are observed by Raman spectroscopy. Moreover, galvanostatic
cycling tests has been performed using the SnO cabbages as anode
material of lithium ion battery and the electrochemical results suggest
that the synthesized SnO cabbage structures are a promising anode
material for lithium ion batteries.