Abstract: In this paper, a simple microfluidic device for monitoring algal cell behavior is proposed. An array of algal microwells is fabricated by PDMS soft-lithography using X-ray LIGA mold, placed on a glass substrate. Two layers of replicated PDMS and substrate are attached by oxygen plasma bonding, creating a microchannel for the microfluidic system. Algal cell are loaded into the microfluidic device, which provides positive charge on the bottom surface of wells. Algal cells, which are negative charged, can be attracted to the bottom of the wells via electrostatic interaction. By varying the concentration of algal cells in the loading suspension, it is possible to obtain wells with a single cell. Liquid medium for cells monitoring are flown continuously over the wells, providing nutrient and waste exchange between the well and the main flow. This device could lead to the uncovering of the quantitative biology of the algae, which is a key to effective and extensive algal utilizations in the field of biotechnology, food industry and bioenergy research and developments.
Abstract: This paper presents the experimental results on
artificial ageing test of 22 kV XLPE cable for distribution system
application in Thailand. XLPE insulating material of 22 kV cable
was sliced to 60-70 μm in thick and was subjected to ac high voltage
at 23
Ôùª
C, 60
Ôùª
C and 75
Ôùª
C. Testing voltage was constantly applied to
the specimen until breakdown. Breakdown voltage and time to
breakdown were used to evaluate life time of insulating material.
Furthermore, the physical model by J. P. Crine for predicts life time
of XLPE insulating material was adopted as life time model and was
calculated in order to compare the experimental results. Acceptable
life time results were obtained from Crine-s model comparing with
the experimental result. In addition, fourier transform infrared
spectroscopy (FTIR) for chemical analysis and scanning electron
microscope (SEM) for physical analysis were conducted on tested
specimens.