Abstract: In this paper, a simple microfluidic device for monitoring algal cell behavior is proposed. An array of algal microwells is fabricated by PDMS soft-lithography using X-ray LIGA mold, placed on a glass substrate. Two layers of replicated PDMS and substrate are attached by oxygen plasma bonding, creating a microchannel for the microfluidic system. Algal cell are loaded into the microfluidic device, which provides positive charge on the bottom surface of wells. Algal cells, which are negative charged, can be attracted to the bottom of the wells via electrostatic interaction. By varying the concentration of algal cells in the loading suspension, it is possible to obtain wells with a single cell. Liquid medium for cells monitoring are flown continuously over the wells, providing nutrient and waste exchange between the well and the main flow. This device could lead to the uncovering of the quantitative biology of the algae, which is a key to effective and extensive algal utilizations in the field of biotechnology, food industry and bioenergy research and developments.
Abstract: Inthis paper, design and fabrication of vertical
micromirror for optical switching applications of single mode optical
fibers are proposed. The structure of micromirror will be created
from negative photoresist (SU-8) on X-ray lithography using X-ray
from synchrotron light source. The properties of X-ray from
synchrotron light source are high-energy electrons which can
construct materials that have a high aspect ratio. In addition, the
technique of gold coating of reflective material has been used for
change direction of light between two pairs of optical fibers. At a
wavelength of 1310 nm with minimum average loss of 5.305 dB is
obtained.