Bread Quality Improvement with Special Novel Additives

Presently a significant portion of the Earth's population does not have access to healthy food. Either because they cannot afford it or because they do not know which one are they. The aim of the VII th Framework Chance project (Nr. 266331) supported by the European Union has been to develop relatively cheap food with favourable nutritional value and it should have acceptable quality for consumers. As one task of the project we manufactured bread products as a basic food. We examined the enrichment of bread products with four kinds of bran, with a special milling product of grain industry (aleurone-rich flour) and with a soy-based sprouted additive. The applied concentration of the six mentioned additives has been optimized and the physical properties of the bread products were monitored. The weight/density of the enriched breads increased a bit, however the volume and height decreased slightly compared to the corresponding data of the control bread. The optimized composition of the final product is favourably affected by these additives having highly preferred composition from nutritional point of view.

A Research about Determination of the Quality of Feed Oils Used as Mixed Feed Raw Material from Some Feed Factories in Konya-Turkey

Feed oil samples which are used as mixed feed raw material were taken from six different feed factories in March, May and July. All factories make production in Konya, Turkey and all of the samples were crude soybean oils. Physical and chemical analyses, free radical scavenger effect, and total phenol content were determined on these oil samples. Moisture (M) content was found between 0.10-22.23%, saponification number (SF) was determined 143.13 to 167.93 KOH/kg, free fatty acidity (FFA) was varied 0.73 to 35.00%, peroxide value (PV) was found between 1.53 and 28.43 meq/kg, unsaponifiable matter (USM) was determined from 0.40 to 17.10%, viscosity (V) was found between 34.30 and 625.67 mPas, sediment (S) amount was determined between 0.60-18.16%, free radical scavenger effect (FRSE) was varied 20.7 to 43.04% inhibition of the extract and total phenol (TPC) content was found between 1.20 and 2.69mg/L extract. Different results were found between months and factories.

Quality Characterization of Burger Affected by Soybean Additives (Natto & Protein Hydrolysate) and Ascorbic Acid

Soy protein is a common ingredient added to processed meats to enhance its functional characteristics. In our study, soybean products (fermented soy Natto and protein hydrolysate) containing hydrolyzed peptides and amino acids, with or without ascorbic acid were added to burger in order to improve its quality characteristics. Results showed that soy additives significantly increased moisture and protein content and reduced (P < 0.05) fat values. Ash content did not affect with Natto additive. Color tools, lightness and yellowness were higher (P

Field Application of Reduced Crude Conversion Spent Lime

Gypsum is being applied to ameliorate subsoil acidity and to overcome the problem of very slow lime movement from surface lime applications. Reduced Crude Conversion Spent Lime (RCCSL) containing anhydrite was evaluated for use as a liming material with specific consideration given to the movement of sulfate into the acid subsoil. Agricultural lime and RCCSL were applied at 0, 0.5, 1.0, and 1.5 times the lime requirement of 6.72 Mg ha-1 to an acid Trappist silt loam (TypicHapuldult). Corn [Zea mays (L.)]was grown following lime material application and soybean [Glycine max (L.) Merr.]was grown in the second year.Soil pH increased rapidly with the addition of the RCCSL material. Over time there was no difference in soil pH between the materials but there was with increasing rate. None of the observed changes in plant nutrient concentration had an impact on yield. Grain yield was higher for the RCCSL amended treatments in the first year but not in the second. There was a significant increase in soybean grain yield from the full lime requirement treatments over no lime.

Utilization of Bioactive Components Produced from Fermented Soybean (Natto) in Beef Burger

Soybean Natto powder was added to the burger in order to enhance the oxidative stability as well as decreases the microbial spoilage. The soybean bioactives compound (soybean Natto) as antioxidant and antimicrobial were added at level of 1, 2 and 3%. Chemical analysis and physical properties were affected by soybean Natto addition. All the tested soybean Natto additives showed strong antioxidant properties. The microbiological indicators were significantly (P < 0.05) affected by the addition of the soybean Natto. Decreasing trends of different extent were also observed in samples of the treatments for total viable counts, Coliform, Staphylococcus aureus, yeast and molds. Storage period was significantly (P < 0.05) affected on microbial counts in all samples Staphylococcus aureus were the most sensitive microbe followed by Coliform group of the sample containing soybean Natto. Sensory attributes were also performed, added soybean Natto exhibits beany flavor which was clear about samples of 3% soybean Natto.

Levels of Some Antinutritional Factors in Tempeh Produced From Some Legumes and Jojobas Seeds

Three legumes i.e. soybean, kidney bean and mung bean, and jojoba seed as an oil seed were processed into tempeh, a fermented food. Changes in phytic acid, total phenols and trypsin inhibitor were monitored during the pretreatments (soaking, soaking– dehulling, washing and cooking) and fermentation with Rhizopus oligosporus. Soaking was found to reduce total phenol and trypsin inhibitor levels in soybean, kidney bean and mung bean. However, phytic acid was reduced by soaking in kidney bean and mung bean. Cooking was the most effective in reducing the activity of trypsin inhibitor. During fermentation, a slight increase in the level of trypsin inhibitor was noticed in soybean. Phytic acid and total phenols were decreased during fermentation in soybean, kidney bean but mung bean faild to form tempeh because the antifungal activity of herein a protein in mung bean, which exerts both chitinase activity and antifungal activity against a variety of fungal species. On the other hand, solid- state fermentation of jojoba seeds was not effective in reducing their content from cyanogenic glycosides (simmondsin).

Effects of Soybean Methyl Ester on the Performance Characteristics of Compression Ignition Engine

Depletion and hazardous gas emissions associated with fossil fuels have caused scientists and global attention to focus on the use of “alternative, eco-friendly substitutes for use in Compression Ignition Engines. In this work, biodiesel was produced by trans-esterification of soybean obtained from a Nigerian market using Sodium Hydroxide (NaOH) as a catalyst.” After the production, the physical properties (specific gravity to kinematic viscosity and net calorific value) of the Soybean-biodiesel produced and petrol diesel obtained from a filling station in Nigeria were determined, and these properties conform to conventional standards (ASTM). A cummins-6V-92TA DDEC diesel (Compression ignition, CI) engine was run on various biodiesel-petrol diesel blends (0/100, 10/90, 20/80, 30/70 and 40/60), the B20 (blend 20/80) was found to be the most satisfactory.

Life Cycle Assessment Comparison between Methanol and Ethanol Feedstock for the Biodiesel from Soybean Oil

As the limited availability of petroleum-based fuel has been a major concern, biodiesel is one of the most attractive alternative fuels because it is renewable and it also has advantages over the conventional petroleum-base diesel. At Present, productions of biodiesel generally perform by transesterification of vegetable oils with low molecular weight alcohol, mainly methanol, using chemical catalysts. Methanol is petrochemical product that makes biodiesel producing from methanol to be not pure renewable energy source. Therefore, ethanol as a product produced by fermentation processes. It appears as a potential feed stock that makes biodiesel to be pure renewable alternative fuel. The research is conducted based on two biodiesel production processes by reacting soybean oils with methanol and ethanol. Life cycle assessment was carried out in order to evaluate the environmental impacts and to identify the process alternative. Nine mid-point impact categories are investigated. The results indicate that better performance on abiotic depletion potential (ADP) and acidification potential (AP) are observed in biodiesel production from methanol when compared with biodiesel production from ethanol due to less energy consumption during the production processes. Except for ADP and AP, using methanol as feed stock does not show any advantages over biodiesel from ethanol. The single score method is also included in this study in order to identify the best option between two processes of biodiesel production. The global normalization and weighting factor based on ecotaxes are used and it shows that producing biodiesel form ethanol has less environmental load compare to biodiesel from methanol.

Plants and Microorganisms for Phytoremediation of Soils Polluted with Organochlorine Pesticides

The goal of presented work is the development phytoremediation method targeted to cleaning environment polluted with organochlorine pesticides, based on joint application of plants and microorganisms. For this aim the selection of plants and microorganisms with corresponding capabilities towards three organochlorine pesticides (Lindane, DDT and PCP) has been carried out. The tolerance of plants to tested pesticides and induction degree of plant detoxification enzymes by these compounds have been used as main criteria for estimating the applicability of plants in proposed technology. Obtained results show that alfalfa, maize and soybean among tested six plant species have highest tolerance to pesticides. As a result of screening, more than 30 strains from genera Pseudomonas have been selected. As a result of GC analysis of incubation area, 11 active cultures for investigated pesticides are carefully chosen.

Effect of Different Tillage Systems on Soil Properties and Production on Wheat, Maize and Soybean Crop

Soil tillage systems can be able to influence soil compaction, water dynamics, soil temperature and crop yield. These processes can be expressed as changes of soil microbiological activity, soil respiration and sustainability of agriculture. Objectives of this study were: 1 - to assess the effects of tillage systems (Conventional System (CS), Minimum Tillage (MT), No-Tillage (NT)) on soil compaction, soil temperature, soil moisture and soil respiration and 2- to establish the effect of the changes on the production of wheat, maize and soybean. Five treatments were installed: CS-plough; MT-paraplow, chisel, rotary grape; NT-direct sowing. The study was conducted on an Argic-Stagnic Faeoziom. The MT and NT applications reduce or completely eliminate the soil mobilization, due to this; soil is compacted in the first year of application. The degree of compaction is directly related to soil type and its state of degradation. The state of soil compaction diminished over time, tending toward a specific type of soil density. Soil moisture was higher in NT and MT at the time of sowing and in the early stages of vegetation and differences diminished over time. Moisture determinations showed statistically significant differences. The MT and NT applications reduced the thermal amplitude in the first 15cm of soil depth and increased the soil temperature by 0.5-2.20C. Water dynamics and soil temperature showed no differences on the effect of crop yields. The determinations confirm the effect of soil tillage system on soil respiration; the daily average was lower at NT (315-1914 mmoli m-2s-1) and followed by MT (318-2395 mmoli m-2s-1) and is higher in the CS (321-2480 mmol m-2s-1). Comparing with CS, all the four conservation tillage measures decreased soil respiration, with the best effects of no-tillage. Although wheat production at MT and NT applications, had no significant differences soybean production was significantly affected from MT and NT applications. The differences in crop yields are recorded at maize and can be a direct consequence of loosening, mineralization and intensive mobilization of soil fertility.

Efficacy and Stability of Ceramic Powder to Inactivate Avian Influenza Virus

This experiment aims to demonstrate the efficacy of ceramic powder derived from various sources to inactivate avian influenza virus and its possibility to use in the environment. The ceramics used in the present experiment were derived from chicken feces (CF), scallop shell (SS), polyvinyl chloride (PVC) and soybean (SB). All ceramics were mixed with low pathogenic AIV (LPAIV) H7N1, and then kept at room temperature. The recovered virus was titrated onto Madin-Darby canine kidney (MDCK) cells. All ceramics were assessed the inactivation stability in the environment by keeping under sunlight and under wet-dry condition until reached 7 week or 7 resuspension times respectively. The results indicate that all ceramics have excellent efficacy to inactivate LPAIV. This efficacy can be maintained under the simulated condition. The ceramics are expected to be the good materials for application in the biosecurity system at farms.

Higher Plants Ability to Assimilate Explosives

The ability of agricultural and decorative plants to absorb and detoxify TNT and RDX has been studied. All tested 8 plants, grown hydroponically, were able to absorb these explosives from water solutions: Alfalfa > Soybean > Chickpea> Chikling vetch >Ryegrass > Mung bean> China bean > Maize. Differently from TNT, RDX did not exhibit negative influence on seed germination and plant growth. Moreover, some plants, exposed to RDX containing solution were increased in their biomass by 20%. Study of the fate of absorbed [1-14ðí]-TNT revealed the label distribution in low and high-molecular mass compounds, both in roots and above ground parts of plants, prevailing in the later. Content of 14ðí in lowmolecular compounds in plant roots are much higher than in above ground parts. On the contrary, high-molecular compounds are more intensively labeled in aboveground parts of soybean. Most part (up to 70%) of metabolites of TNT, formed either by enzymatic reduction or oxidation, is found in high molecular insoluble conjugates. Activation of enzymes, responsible for reduction, oxidation and conjugation of TNT, such as nitroreductase, peroxidase, phenoloxidase and glutathione S-transferase has been demonstrated. Among these enzymes, only nitroreductase was shown to be induced in alfalfa, exposed to RDX. The increase in malate dehydrogenase activities in plants, exposed to both explosives, indicates intensification of Tricarboxylic Acid Cycle, that generates reduced equivalents of NAD(P)H, necessary for functioning of the nitroreductase. The hypothetic scheme of TNT metabolism in plants is proposed.

Acceptance of Consumer on Various Tempeh and Protein Content Comparison

This research aims to study consumer acceptance of Tempeh from various raw materials (type of bean) and determine protein contents for comparison. Tempeh made from soybean, peanut, white kidney bean and sesame in the ratio: - soybean:sesame =1:0.1, soybean:white kidney:sesame =1:1:0.1, soybean:peanut:sesame =1:1:0.1 and peanut:white kidney bean: sesame =1:1:0.1. The study found that consumer is most satisfied with appearances on soybean mixed with white kidney and black sesame tempeh (3.98). The most satisfied tempeh with textures is soybean mixed with peanut and black sesame tempeh (4.00). The most satisfied tempeh with odor is peanut mixed with white kidney bean and black sesame tempeh (4.04). And the most satisfied tempeh with flavor is peanut mixed with white kidney bean and black sesame tempeh (4.2). The amount of protein in production, soybean tempeh has the highest protein. When we add sesame seeds, it made the protein content slightly decreased (1.86 and 0.6 %). When we use peanut as raw material, the protein content decreased 15.3%. And when we use white kidney bean as raw material, the protein content decreased (22.77- 26.11%). 

Mathematical Correlation for Brake Thermal Efficiency and NOx Emission of CI Engine using Ester of Vegetable Oils

The aim of this study is to develop mathematical relationships for the performance parameter brake thermal efficiency (BTE) and emission parameter nitrogen oxides (NOx) for the various esters of vegetable oils used as CI engine fuel. The BTE is an important performance parameter defining the ability of engine to utilize the energy supplied and power developed similarly it is indication of efficiency of fuels used. The esters of cottonseed oil, soybean oil, jatropha oil and hingan oil are prepared using transesterification process and characterized for their physical and main fuel properties including viscosity, density, flash point and higher heating value using standard test methods. These esters are tried as CI engine fuel to analyze the performance and emission parameters in comparison to diesel. The results of the study indicate that esters as a fuel does not differ greatly with that of diesel in properties. The CI engine performance with esters as fuel is in line with the diesel where as the emission parameters are reduced with the use of esters. The correlation developed between BTE and brake power(BP), gross calorific value(CV), air-fuel ratio(A/F), heat carried away by cooling water(HCW). Another equation is developed between the NOx emission and CO, HC, smoke density (SD), exhaust gas temperature (EGT). The equations are verified by comparing the observed and calculated values which gives the coefficient of correlation of 0.99 and 0.96 for the BTE and NOx equations respectively.

Soybean and Fermented Soybean Extract Antioxidant Activities

Today, people are more interested in the foods beneficial on their health. However, there are still lacks of accurate knowledge in the field of biological properties, functional properties, including the application of legume in foods. This study focused on antioxidant activity of soybean (SB) and fermented soybean (FSB) crude extracts evaluating to have more information in fortification SB and FSB crude extracts in food products and/or dietary supplement. SB and FSB crude extracts were prepared by infusion with water and ethanol. The antioxidant activity of crude extracts was studied with DPPH and ABTS assay including commercial standard. From both DPPH and ABTS assay, the antioxidant activity of SB and FSB water crude extract showed higher antioxidant activity than ethanol crude extract, and FSB crude extract showed higher antioxidant activity than SB crude extract. In DPPH assay, BHT and vitamin C showed IC50 values at 0.241, 0.039 mg/ml, in ABTS assay. In addition, Trolox showed IC50 at 0.058 mg/ml respectively. FSB water crude extract showed high antioxidant activity. Finally, the functional properties study of both water and ethanol crude extracts should be done for beneficial in application of these extracts in food products and dietary supplement in the near future.

Identification Characterization and Production of Phytase from Endophytic Fungi

Phytases are acid phosphatase enzymes, which efficiently cleave phosphate moieties from phytic acid, thereby generating myo-inositol and inorganic phosphate. Thirty four isolates of endophytic fungi to produce of phytases were isolated from leaf, stem and root fragments of soybean. Screening of 34 isolates of endophytic fungi identified the phytases produced by Rhizoctonia sp. and Fusarium verticillioides . The phytase production were the best induced by phytic acid and rice bran compared the others inducer in submerged fermentation medium used. The phytase produced by both Rhizoctonia sp. and F. verticillioides have pH optimum at 4.0 and 5.0 respectively. The characterization of phytase from Fusarium verticillioides showed that temperature optimum was 500C and stability until 600C, the pH optimum 5.0 and pH stability was 2.5 – 6.0, and substrate specificity were rice bran>soybean meal>corn> coconut cake, respectively.

Simulation of Increased Ambient Ozone to Estimate Nutrient Content and Genetic Change in Two Thai Soybean Cultivars

This research studied the simulation of increased ambient ozone to estimate nutrient content and genetic changes in two Thai soybean cultivars (Chiang Mai 60 and Srisumrong 1). Ozone stress conditions affected proteins and lipids. It was found that proteins decreased, but lipids increased. Srisumrong 1 cultivars were more sensitive to ozone stress than Chiang Mai 60 cultivars. The effect of ozone stress conditions on plant phenotype and genotype was analyzed using the AFLP technique for the 2 Thai soybean cultivars (Chiang Mai 60 and Srisumrong 1).

The Use of Acid-Aluminium Tolerant Bradyrhizobium japonicum Formula for

Land with low pH soil spread widely in Indonesia can be used for soybean (Glycine max) cultivation, however the production is low. The use of acid tolerant soybean and acidaluminium tolerant nitrogen-fixing bacteria formula was an alternative way to increase soybean productivity on acid soils. Bradyrhizobium japonicum is one of the nitrogen fixing bacteria which can symbiose with soybean plants through root nodule formation. Most of the nitrogen source required by soybean plants can be provided by this symbiosis. This research was conducted to study the influence of acid-aluminium tolerant B. japonicum strain BJ 11 formula using peat as carrier on growth of Tanggamus and Anjasmoro cultivar soybean planted on acid soil fields (pH 5.0- 5.5). The results showed that the inoculant was able to increase the growth and production of soybean which were grown on fields acid soil at Sukadana (Lampung) and Tanah Laut (South Kalimantan), Indonesia.

Biodiesel Production over nano-MgO Supported on Titania

Nano-MgO was successfully deposited on titania using deposition-precipitation method. The catalyst produced was characterised using FTIR, XRD, BET and XRF and its activity was tested on the transesterification reaction of soybean oil to biodiesel. The catalyst activity improved when the reaction temperature was increasedfrom 150 and 225 OC. It was also observed that increasing the reaction time above 1h had no significant benefit on conversion. The stability fixed MgO on TiO2 was investigated using XRF and ICP-OES. It was observed that MgO loss during the reaction was between 0.5-2.3 percent and that there was no correlation between the reaction temperature and the MgO loss.

Characterization of Antioxidant Peptides of Soybean Protein Hydrolysate

In order to characterize the soy protein hydrolysate obtained in this study, gel chromatography on Sephadex G-25 was used to perform the separation of the peptide mixture and electrophoresis in SDS-polyacrylamide gel has been employed. Protein hydrolysate gave high antioxidant activities, but didn't give any antimicrobial activities. The antioxidant activities of protein hydrolysate was in the same trend of peptide content which gave high antioxidant activities and high peptide content between fractions 15 to 50. With increasing peptide concentrations, the scavenging effect on DPPH radical increased until about 70%, thereafter reaching a plateau. In compare to different concentrations of BHA, which exhibited higher activity (90%), soybean protein hydrolysate exhibited high antioxidant activities (70%) at a concentration of 1.45 mg/ml at fraction 25. Electrophoresis analysis indicated that, low- MW hydrolysate fractions (F1) appeared, on average, to have higher DPPH scavenging activities than high-MW fractions. These results revealed that soybean peptides probably contain substances that were proton donors and could react with free radicals to convert them to stable diamagnetic molecules.