Abstract: Antioxidant-rich extracts were prepared from pomegranate peels, seeds and juice using methanol and ethanol and their antioxidant activity was evaluated by the 1,1-diphenyl-2-picrylhydrazine (DPPH) radical scavenging and Ferric Reducing Antioxidant Power (FRAP) method. Both analytical methods indicated a higher antioxidant activity in extracts prepared from peels, which was comparable to that of butylated hydroxytoluene (BHT). Furthermore, the antioxidant activity was correlated to the phenolic and flavonoid content of the various extracts. The antioxidant effectiveness of the extracts was also assessed using corn oil as the oxidation substrate. More specifically, preheated corn oil samples stabilized with extracts at a concentration of 250 ppm, 500 ppm or 1,000 ppm were subjected to accelerated aging (100 oC, 10 days) and the extent of oxidative alteration was followed by the measurement of the peroxide, conjugated dienes and trienes, as well as p-aniside value. BHT at its legal limit (200 ppm) served as standard besides the control sample. Results from the different parameters were in agreement with each other suggesting that pomegranate extracts can stabilize corn oil effectively under accelerated conditions, at all concentrations tested. However, the magnitude of oil stabilization depended strongly on the amount of extract added and this was positively correlated with their phenolic content. Pomegranate peel extracts, which exhibited the highest not only phenolic and flavonoid content but also antioxidant activity, were more potent in inhibiting oxidative deterioration. Both methanolic and ethanolic peel extracts at a concentration of 500 ppm exerted a stabilizing effect comparable to that of BHT, while at a concentration of 1000 ppm they exhibited higher stabilization efficiency in comparison to BHT. Finally, heating oil samples resulted in a time dependent decrease in their antioxidant capacity. Samples containing peel extracts appeared to retain their antioxidant capacity for a longer period, indicating that these extracts contained active compounds that offered superior antioxidant protection to corn oil.
Abstract: In this work was developed and implemented a thermal fogging disinfection system to counteract pathogens from poultry feces in agribusiness farms, to reduce mortality rates and increase biosafety in them. The control system consists of two phases for the conditioning of the farm during the sanitary break. In the first phase, viral and bacterial inactivation was performed by treating the stool dry cleaning, along with the development of a specialized product that foster the generation of temperatures above 55 °C in less than 24 hr, for virus inactivation. In the second phase, a process for disinfection by fogging was implemented, along with the development of a specialized disinfectant that guarantee no risk for the operators’ health or birds. As a result of this process, it was possible to minimize the level of mortality of chickens on farms from 12% to 5.49%, representing a reduction of 6.51% in the death rate, through the formula applied to the treatment of poultry litter based on oxidising agents used as antiseptics, hydrogen peroxide solutions, glacial acetic acid and EDTA in order to act on bacteria, viruses, micro bacteria and spores.
Abstract: Diabetes mellitus (DM) is a metabolic disease that can be indicated by the high level of blood glucose. The objective of this study was to observe the antidiabetic properties of ethanolic extract of Indonesian Swietenia mahagoni Jacq. seed on the profile of pancreatic superoxide dismutase and β-cells in the alloxan- experimental diabetic rats. The Swietenia mahagoni seed was obtained from Leuwiliang-Bogor, Indonesia. Extraction of Swietenia mahagoni was done by using ethanol with maceration methods. A total of 25 male Sprague dawley rats were divided into five groups; (a) negative control group, (b) positive control group (DM), (c) DM group that was treated with Swietenia mahagoni seed extract, (d) DM group that was treated with acarbose, and (e) non-DM group that was treated with Swietenia mahagoni seed extract. The DM groups were induced by alloxan (110 mg/kgBW). The extract was orally administrated to diabetic rats 500 mg/kg/BW/day for 28 days. The extract showed hypoglycemic effect, increased body weight, increased the content of superoxide dismutase in the pancreatic tissue, and delayed the rate of β-cells damage of experimental diabetic rats. These results suggested that the ethanolic extract of Indonesian Swietenia mahagoni Jacq. seed could be proposed as a potential anti-diabetic agent.
Abstract: The practical application of the Fenton-based treatment method for organic compounds-contaminated water purification is limited mainly because of the large amount of ferric sludge formed during the treatment, where ferrous iron (Fe(II)) is used as the activator of the hydrogen peroxide oxidation processes. Reuse of ferric sludge collected from clarifiers to substitute Fe(II) salts allows reducing the total cost of Fenton-type treatment technologies and minimizing the accumulation of hazardous ferric waste. Dissolution of ferric iron (Fe(III)) from the sludge to liquid phase at acidic pH and autocatalytic transformation of Fe(III) to Fe(II) by phenolic compounds (tannic acid, lignin, phenol, catechol, pyrogallol and hydroquinone) added or present as water/wastewater constituents were found to be essentially involved in the Fenton-based oxidation mechanism. Observed enhanced formation of highly reactive species, hydroxyl radicals, resulted in a substantial organic contaminant degradation increase. Sludge reuse at acidic pH and in the presence of ferric iron reductants is a novel strategy in the Fenton-based treatment application for organic compounds-contaminated water purification.
Abstract: Aqueous ethanol and aqueous acetone extracts of
Moringa oleifera (outer pericarp of immature fruit and flower) and
Sesbania grandiflora white variety (flower and leaf) were examined
for radical scavenging capacities and antioxidant activities. Ethanol
extract of S. grandiflora (flower and leaf) and acetone extract of M.
oleifera (outer pericarp of immature fruit and flower) contained
relatively higher levels of total dietary phenolics than the other
extracts. The antioxidant potential of the extracts were assessed by
employing different in vitro assays such as reducing power assay,
DPPH˙, ABTS˙+ and ˙OH radical scavenging capacities,
antihemolytic assay by hydrogen peroxide induced method and metal
chelating ability. Though all the extracts exhibited dose dependent
reducing power activity, acetone extract of all the samples were
found to have more hydrogen donating ability in DPPH˙ (2.3% -
65.03%) and hydroxyl radical scavenging systems (21.6% - 77.4%)
than the ethanol extracts. The potential of multiple antioxidant
activity was evident as it possessed antihemolytic activity (43.2 % to
68.0 %) and metal ion chelating potency (45.16 - 104.26 mg EDTA/g
sample). The result indicate that acetone extract of M. oleifera (OPIF
and flower) and S. grandiflora (flower and leaf) endowed with
polyphenols, could be utilized as natural antioxidants/nutraceuticals.
Abstract: The increase of technogenic and natural accidents,
accompanied by air pollution, for example, by combustion products,
leads to the necessity of respiratory protection. This work is devoted to the development of a calorimetric method
and a device which allows investigating quickly the kinetics of
carbon dioxide sorption by chemisorbents on the base of potassium
superoxide in order to assess the protective properties of respiratory
protective closed circuit apparatus. The features of the traditional approach for determining the
sorption properties in a thin layer of chemisorbent are described, as
well as methods and devices, which can be used for the sorption
kinetics study. The authors developed an approach (as opposed to the traditional
approach) based on the power measurement of internal heat sources
in the chemisorbent layer. The emergence of the heat sources is a
result of exothermic reaction of carbon dioxide sorption. This
approach eliminates the necessity of chemical analysis of samples
and can significantly reduce the time and material expenses during
chemisorbents testing. Error of determining the volume fraction of adsorbed carbon
dioxide by the developed method does not exceed 12%. Taking into
account the efficiency of the method, we consider that it is a good
alternative to traditional methods of chemical analysis under the
assessment of the protection sorbents quality.
Abstract: This paper reports on the response of a fiber-optic
sensing probe to small concentrations of hydrogen peroxide (H2O2)
vapor at room temperature. H2O2 has extensive applications in industrial and medical
environments. Conversely, H2O2 can be a health hazard by itself. For
example, H2O2 induces cellular damage in human cells and its
presence can be used to diagnose illnesses such as asthma and human
breast cancer. Hence, development of reliable H2O2 sensor is of vital
importance to detect and measure this species. Ferric ferrocyanide, referred to as Prussian Blue (PB), was
deposited on the tip of a multimode optical fiber through the single
source precursor technique and served as an indicator of H2O2 in a
spectroscopic manner. Sensing tests were performed in H2O2-H2O
vapor mixtures with different concentrations of H2O2. The results of sensing tests show the sensor is able to detect H2O2
concentrations in the range of 50.6 ppm to 229.5 ppm. Furthermore,
the sensor response to H2O2 concentrations is linear in a log-log scale
with the adjacent R-square of 0.93. This sensing behavior allows us
to detect and quantify the concentration of H2O2 in the vapor phase.
Abstract: Low Temperature Matrix Isolation - Electron
Paramagnetic Resonance (LTMI-EPR) Spectroscopy was utilized to
identify the species of iron oxide nanoparticles generated during the
oxidative pyrolysis of 1-methylnaphthalene (1-MN). The otherwise
gas-phase reactions of 1--MN were impacted by a polypropylenimine
tetra-hexacontaamine dendrimer complexed with iron (III) nitrate
nonahydrate diluted in air under atmospheric conditions. The EPR
fine structure of Fe (III)2O3 nanoparticles clusters, characterized by gfactors
of 2.00, 2.28, 3.76 and 4.37 were detected on a cold finger
maintained at 77 K after accumulation over a multitude of
experiments. Additionally, a high valence Fe (IV) paramagnetic
intermediate and superoxide anion-radicals, O2•- adsorbed on
nanoparticle surfaces in the form of Fe (IV) --- O2•- were detected
from the quenching area of Zone 1 in the gas-phase.
Abstract: This study aimed to determine the possible protective effects of L‐carnitine against gentamicin‐induced nephrotoxicity. Forty male albino rats were divided into 4 groups (10 rats each); Group 1: normal control, group 2: induced nephrotoxicity (gentamicin 50 mg/kg/day S.C; 8 days), group 3: treated with L‐ carnitine (40 mg/kg/d SC for 12 days) and group 4: treated with L‐ carnitine 4 days before and for 8 days in concomitant with gentamicin. Gentamicin‐induced nephrotoxicity (group 2): caused significant increase in serum urea, creatinine, urinary N‐acetyl‐B‐D‐ glucosaminidase (NAG), gamma glutamyl transpeptidase (GGT), urinary total protein and kidney tissue malondialdehyde (MDA) with significant decrease in serum superoxide dismutase (SOD), serum catalase and creatinine clearance and marked tubular necrosis in the proximal convoluted tubules with interruption in the basement membrane around the necrotic tubule compared to the normal control group. L‐carnitine 4 days before and for 8 days in concomitant with gentamicin (group 4) offered marked decrease in serum urea, serum creatinine, urinary NAG, urinary GGT, urinary proteins and kidney tissue MDA, with marked increase in serum SOD, serum catalase and creatinine clearance with marked improvement in the tubular damage compared to gentamicin‐induced nephrotoxicity group. L‐carnitine administered for 12 days produced no change in the parameters mentioned above as compared to the normal control group. In conclusion: L‐carnitine could reduce most of the biochemical parameters and also improve the histopathological features of kidney asscociated with gentamicin induced‐nephrotoxicity.
Abstract: Feed oil samples which are used as mixed feed raw
material were taken from six different feed factories in March, May
and July. All factories make production in Konya, Turkey and all of
the samples were crude soybean oils. Physical and chemical analyses,
free radical scavenger effect, and total phenol content were
determined on these oil samples. Moisture (M) content was found
between 0.10-22.23%, saponification number (SF) was determined
143.13 to 167.93 KOH/kg, free fatty acidity (FFA) was varied 0.73 to
35.00%, peroxide value (PV) was found between 1.53 and 28.43
meq/kg, unsaponifiable matter (USM) was determined from 0.40 to
17.10%, viscosity (V) was found between 34.30 and 625.67 mPas,
sediment (S) amount was determined between 0.60-18.16%, free
radical scavenger effect (FRSE) was varied 20.7 to 43.04% inhibition
of the extract and total phenol (TPC) content was found between 1.20
and 2.69mg/L extract. Different results were found between months
and factories.
Abstract: Non-water based fixed abrasive polishing was adopted
to manufacture LBO crystal for nano precision surface quality because
of its deliquescent. Ethyl alcohol was selected as the non-water based
slurry solvent and ethanediamine, lactic acid, hydrogen peroxide was
added in the slurry as a chemical additive, respectively. Effect of
different additives with non-water based slurry on material removal
rate, surface topography, microscopic appearances, and surface
roughness were investigated in fixed abrasive polishing of LBO
crystal. The results show the best surface quality of LBO crystal with
surface roughness Sa 8.2 nm and small damages was obtained by
non-water based slurry with lactic acid. Non-water based fixed
abrasive polishing can achieve nano precision surface quality of LBO
crystal with high material removal.
Abstract: Epidermal Growth Factor (EGF, Mw=6,045) has been
reported to have high efficiency of wound repair and anti-wrinkle
effect. However, the half-life of EGF in the body is too short to exert
the biological activity effectively when applied in free form. Growth
Factors can be stabilized by immobilization with carbohydrates from
thermal and proteolytic degradation. Low molecular weight chitosan
(LMCS) and its derivate prepared by hydrogen peroxide has high
solubility. LM6A6DC was successfully prepared as a reactive
carbohydrate for the stabilization of EGF by the reactions of LMCS
with alkalization, tosylation, azidation and reduction. The structure of
LM6A6DC was confirmed by FT-IR, 1H NMR and elementary
analysis. For enhancing the stability of free EGF, EGF was attached
with LM6A6DC by using water-soluble carbodiimide.
EGF-LM6A6DC conjugates did not show any cytotoxicity on the
Normal Human Dermal Fibroblast (NHDF) 3T3 proliferation at least
under 100 μg/ml. In the result, it was considered that LM6A6DC is
suitable to immobilize of growth factor.
Abstract: Diminished antioxidant defense or increased
production of reactive oxygen species in the biological system can
result in oxidative stress which may lead to various
neurodegenerative diseases including Alzheimer’s disease (AD).
Microglial activation also contributes to the progression of AD by
producing several proinflammatory cytokines, nitric oxide (NO) and
prostaglandin E2 (PGE2). Oxidative stress and inflammation have
been reported to be possible pathophysiological mechanisms
underlying AD. In addition, the cholinergic hypothesis postulates that
memory impairment in patient with AD is also associated with the
deficit of cholinergic function in the brain. Although a number of
drugs have been approved for the treatment of AD, most of these
synthetic drugs have diverse side effects and yield relatively modest
benefits. Marine algae have great potential in pharmaceutical and
biomedical applications as they are valuable sources of bioactive
properties such as anticoagulation, antimicrobial, antioxidative,
anticancer and anti-inflammatory. Hence, this study aimed to provide
an overview of the properties of Malaysian seaweeds (Padina
australis, Sargassum polycystum and Caulerpa racemosa) in
inhibiting oxidative stress, neuroinflammation and cholinesterase
enzymes. These seaweeds significantly exhibited potent DPPH and
moderate superoxide anion radical scavenging ability (P
Abstract: The rhizome of Java grass, Cyperus rotundus was
extracted different organic polar and non-polar solvents and
performed the in vitro antiviral and immunostimulant activities
against White Spot Syndrome Virus (WSSV) and Vibrio harveyi
respectively. Based on the initial screening the ethyl acetate extract of
C. rotundus was strong activities and further it was purified through
silica column chromatography and the fractions were screened again
for antiviral and immunostimulant activity. Among the different
fractions screened against the WSSV and V. harveyi, the fractions, FIII
to FV had strong activities. In order to study the in vivo influence
of C. rotundus, the fractions (F-III to FV) were pooled and delivered
to the F. indicus through artificial feed for 30 days. After the feeding
trail the experimental and control diet fed F. indicus were challenged
with virulent WSSV and studied the survival, molecular diagnosis,
biochemical, haematological, and immunological parameters.
Surprisingly, the pooled fractions (F-IV to FVI) incorporated diets
helped to significantly (P
Abstract: Analysis of the properties of coconut (Cocos nucifera)
and its oil was evaluated in this work using standard analytical
techniques. The analyses carried out include proximate composition
of the fruit, extraction of oil from the fruit using different process
parameters and physicochemical analysis of the extracted oil. The
results showed the percentage (%) moisture, crude lipid, crude
protein, ash and carbohydrate content of the coconut as 7.59, 55.15,
5.65, 7.35 and 19.51 respectively. The oil from the coconut fruit was
odourless and yellowish liquid at room temperature (30oC). The
treatment combinations used (leaching time, leaching temperature
and solute: solvent ratio) showed significant differences (P
Abstract: The effects of basil and/or chamomile seed
supplementation on the growth of Hubbard broiler chicks were
evaluated. The antioxidant effects of these supplements were also
assessed. 120 1-day-old broiler chicks were randomly divided into
four equal groups. The control group (group 1) was fed a basal diet
(BD) without supplementation. Groups 2, 3, and 4 were fed the BD
supplemented with 10g basil, 10g chamomile, and 5g basil plus 5g
chamomile per kg of food, respectively. Basil supplementation alone
or in combination with chamomile non-significantly (P≥0.05)
increased final body weight (3.2% and 0.3%, respectively) and
weight gain (3.5% and 3.6%, respectively) over the experimental
period. Chamomile supplementation alone non-significantly (P≥0.05)
reduced final body weight and weight gain over the experimental
period by 1.7% and 1.7%, respectively. In comparison to the control
group, herbal seed supplementation reduced feed intake and
improved the feed conversion and protein efficiency ratios. In
general, basil seed supplementation stimulated chicken growth and
improved the feed efficiency more effectively than chamomile seed
supplementation. The antioxidant activities of basil and/or chamomile
supplementation were examined in the thymus, bursa, and spleen. In
chickens that received supplements, the level of malondialdehyde
was significantly decreased, whereas the activities of glutathione,
superoxide dismutase, and catalase were significantly increased
(P
Abstract: In this investigation, we have evaluated the effects of
arsenic trioxide on hepatic function in pregnant and lactating Swiss
albino mice and their suckling pups. Experiments were carried out on
female mice given 175 ppm As2O3 in their drinking water from the
14th day of pregnancy until day 14 after delivery. Our results showed
a significant decrease in plasma levels of total protein and albumin,
cholesterol and triglyceride in As2O3 treated mice and their pups. The
hyperbilirubinemia and the increased plasma total alkaline
phosphatase activity suggested the presence of cholestasis.
Transaminase activities as well as lactate deshydrogenase activity in
plasma, known as biomarkers of hepatocellular injury, were elevated
indicating hepatic cells’ damage after treatment with As2O3.
Exposure to arsenic led to an increase of liver thiobarbituric acid
reactive substances level along with a concomitant decrease in the
activities of superoxide dismutase, catalase and glutathione
peroxidase and in glutathione.
Abstract: The study examined the effect of Bonny Light whole
crude oil (WC) and its water soluble fraction (WSF) on the activities
of antioxidant enzymes (catalase (CAT) and superoxide dismutase
(SOD)) and crude mitochondria ATPases in the radicle of
germinating bean (Vigna unguiculata). The percentage germination,
level of lipid peroxidation, antioxidant enzyme and mitochondria
Ca2+ and Mg2+ ATPase activities were measured in the radicle of
bean after 7, 14 and 21 days post germination. Viable bean seeds
were planted in soils contaminated with 10ml, 25ml and 50ml of
whole crude oil (WC) and its water soluble fraction (WSF) to obtain
2, 5 and 10% v/w crude oil contamination. There was dose dependent
reduction of the number of bean seeds that germinated in the
contaminated soils compared with control (p
Abstract: Ocimum americanum L (Lamiaceae) is an annual herb
that is native to tropical Africa. The in vitro and in vivo antioxidant
activity of its aqueous extract was carefully investigated by assessing
the DPPH radical scavenging activity, ABTS radical scavenging
activity and hydrogen peroxide radical scavenging activity. The
reducing power, total phenol, total flavonoids and flavonols content
of the extract were also evaluated. The data obtained revealed that the
extract is rich in polyphenolic compounds and scavenged the radicals
in a concentration dependent manner. This was done in comparison
with the standard antioxidants such as BHT and Vitamin C. Also, the
induction of oxidative damage with paracetamol (2000 mg/kg)
resulted in the elevation of lipid peroxides and significant (P < 0.05)
decrease in activities of superoxide dismutase, glutathione
peroxidase, glutathione reductase and catalase in the liver and kidney
of rats. However, the pretreatment of rats with aqueous extract of O.
americanum leaves (200 and 400 mg/kg) and silymarin (100 mg/kg)
caused a significant (P < 0.05) reduction in the values of lipid
peroxides and restored the levels of antioxidant parameters in these
organs. These findings suggest that the leaves of O. americanum have
potent antioxidant properties which may be responsible for its
acclaimed folkloric uses.
Abstract: Food is widely packaged with plastic materials to
prevent microbial contamination and spoilage. Ionizing radiation is
widely used to sterilize the food-packaging materials. Sterilization by
γ-radiation causes degradation such as embrittlement, stiffening,
softening, discoloration, odour generation, and decrease in molecular
weight. Many antioxidants can prevent γ-degradation but most of
them are toxic. The migration of antioxidants to its environment
gives rise to major concerns in case of food packaging plastics. In
this attempt, we have aimed to utilize synergistic mixtures of
stabilizers which are approved for food-contact applications.
Ethylene-propylene-diene terpolymer has been melt-mixed with
hindered amine stabilizers (HAS), phenolic antioxidants and organophosphites
(hydroperoxide decomposer). Results were discussed by
comparing the stabilizing efficiency of mixtures with and without
phenol system. Among phenol containing systems where we mostly
observed discoloration due to the oxidation of hindered phenol, the
combination of secondary HAS, tertiary HAS, organo-phosphite and
hindered phenol exhibited improved stabilization efficiency than
single or binary additive systems. The mixture of secondary HAS and
tertiary HAS, has shown antagonistic effect of stabilization.
However, the combination of organo-phosphite with secondary HAS,
tertiary HAS and phenol antioxidants have been found to give
synergistic even at higher doses of Gamma-irradiation. The effects
have been explained through the interaction between the stabilizers.
After γ-irradiation, the consumption of oligomeric stabilizer
significantly depends on the components of stabilization mixture. The
effect of the organo-phosphite antioxidant on the overall stability has
been discussed.