Abstract: In this paper we develop and analyze the model for
the spread of Leptospirosis by age group in Thailand, between 1997
and 2010 by using mathematical modeling and computer simulation.
Leptospirosis is caused by pathogenic spirochetes of the genus
Leptospira. It is a zoonotic disease of global importance and an
emerging health problem in Thailand. In Thailand, leptospirosis is a
reportable disease, the top three age groups are 23.31% in 35-44
years olds group, 22.76% in 25-34 year olds group, 17.60% in 45-54
year olds group from reported leptospirosis between 1997 and 2010,
with a peak in 35-44 year olds group. Our paper, the Leptosipirosis
transmission by age group in Thailand is studied on the mathematical
model. Some analytical and simulation results are presented.
Abstract: Many high-risk pathogens that cause disease in
humans are transmitted through various food items. Food-borne
disease constitutes a major public health problem. Assessment of the
quality and safety of foods is important in human health. Rapid and
easy detection of pathogenic organisms will facilitate precautionary
measures to maintain healthy food. The Polymerase Chain Reaction
(PCR) is a handy tool for rapid detection of low numbers of bacteria.
We have designed gene specific primers for most common food
borne pathogens such as Staphylococci, Salmonella and E.coli.
Bacteria were isolated from food samples of various food outlets and
identified using gene specific PCRs. We identified Staphylococci,
Salmonella and E.coli O157 using gene specific primers by rapid and
direct PCR technique in various food samples. This study helps us in
getting a complete picture of the various pathogens that threaten to
cause and spread food borne diseases and it would also enable
establishment of a routine procedure and methodology for rapid
identification of food borne bacteria using the rapid technique of
direct PCR. This study will also enable us to judge the efficiency of
present food safety steps taken by food manufacturers and exporters.
Abstract: Drinking water is one of the most valuable resources
available to mankind. The presence of pathogens in drinking water is
highly undesirable. Because of the Lateritic soil, the iron
concentrations were high in ground water. High concentration of iron
and other trace elements could restrict bacterial growth and modify
their metabolic pattern as well. The bacterial growth rate reduced in
the presence of iron in water. This paper presents the results of a
controlled laboratory study conducted to assess the inhibition of
micro-organism (pathogen) in well waters in the presence of
dissolved iron concentrations. Synthetic samples were studied in the
laboratory and the results compared with field samples. Predictive
model for microbial inhibition in the presence of iron is presented. It
was seen that the bore wells, open wells and the field results varied,
probably due to the nature of micro-organism utilizing the iron in
well waters.
Abstract: The ability to distinguish missense nucleotide
substitutions that contribute to harmful effect from those that do not
is a difficult problem usually accomplished through functional in
vivo analyses. In this study, instead current biochemical methods, the
effects of missense mutations upon protein structure and function
were assayed by means of computational methods and information
from the databases. For this order, the effects of new missense
mutations in exon 5 of PTEN gene upon protein structure and
function were examined. The gene coding for PTEN was identified
and localized on chromosome region 10q23.3 as the tumor
suppressor gene. The utilization of these methods were shown that
c.319G>A and c.341T>G missense mutations that were recognized in
patients with breast cancer and Cowden disease, could be pathogenic.
This method could be use for analysis of missense mutation in others
genes.
Abstract: Information regarding early onset neonatal sepsis
(EONS) pathogens may vary between regions. Global perspectives
showed Group B Streptococcal (GBS) as the most common causative
pathogens, but the widespread use of intrapartum antibiotics has
changed the pathogens pattern towards gram negative
microorganisms, especially E. coli. Objective of this study is to
describe the pathogens isolated, to assess current treatment and risk
of EONS. Records of 899 neonates born in three General Hospitals
between 2009 until 2012 were retrospectively reviewed. Proven was
found in 22 (3%) neonates. The majority was isolated with gram
positive organisms, 17 (2.3%). All grams positive and most gram
negative organisms showed sensitivity to the tested antibiotics. Only
two rare gram negative organisms showed total resistant. Male was
possible risk of proven EONS. Although proven EONS remains
uncommon in Malaysia, nonetheless, the effect of intrapartum
antibiotics still required continuous surveillance.
Abstract: Studies were carried out to determine the in vitro
susceptibility of the typhoid pathogens to combined action of Euphorbia hirta, Euphorbia heterophylla and Phyllanthus niruri. Clinical isolates of the typhoid bacilli were subjected to susceptibility testing using agar diffusion technique and the minimum inhibitory
concentration (MIC) determined with tube dilution technique. These
isolates, when challenged with doses of the extracts from the three
medicinal plants showed zones of inhibition as wide as 26±0.2mm, 22±0.1mm and 18±0.0mm respectively. The minimum inhibitory concentration (MIC) revealed organisms inhibited at varying
concentrations of extracts: E. hirta (S. typhi 0.250mg/ml, S. paratyphi A 0.125mg/ml, S. paratyphi B 0.185mg/ml and S. paratyphi C 0.225mg/ml), E. heterophylla (S. typhi 0.280mg/ml, S. paratyphi A
0.150mg/ml, S. paratyphi B 0.200mg/ml and S. paratyphi C 0.250mg/ml) and P. niruri (S. typhi 0.150mg/ml, S. paratyphi A 0.100mg/ml, S. paratyphi B 0.115mg/ml and S. paratyphi C 0.125mg/ml). The results of the synergy between the three plants in
the ration of 1:1:1 showed very low MICs for the test pathogens as follows S. typhi 0.025mg/ml, S. paratyphi A 0.080mg/ml, S. paratyphi B 0.015mg/ml and S. paratyphi C 0.10mg/ml with the
diameter zone of inhibition (DZI) ranging from 35±0.2mm,
28±0.4mm, 20±0.1mm and 32±0.3mm respectively. The secondary
metabolites were identified using simple methods and HPLC. Organic components such as anthroquinones, different alkaloids,
tannins, 6-ethoxy-1,2,3,4-tetrahydro-2,2,4-trimethyl and steroids were identified. The prevalence of Salmonellae, a deadly infectious disease, is still very high in parts of Nigeria. The synergistic action of these three plants is very high. It is concluded that pharmaceutical companies should take advantage of these findings to develop new
anti-typhoid drugs from these plants.
Abstract: An alarming emergence of multidrug-resistant strains
of the tuberculosis pathogen Mycobacterium tuberculosis and
continuing high worldwide incidence of tuberculosis has invigorated
the search for novel drug targets. The enzyme glutamate racemase
(MurI) in bacteria catalyzes the stereoconversion of L-glutamate to
D-glutamate which is a component of the peptidoglycan cell wall of
the bacterium. The inhibitors targeted against MurI from several
bacterial species have been patented and are advocated as promising
antibacterial agents. However there are none available against MurI
from Mycobacterium tuberculosis, due to the lack of its threedimensional
structure. This work accomplished two major objectives.
First, the tertiary structure of MtMurI was deduced computationally
through homology modeling using the templates from bacterial
homologues. It is speculated that like in other Gram-positive bacteria,
MtMurI exists as a dimer and many of the protein interactions at the
dimer interface are also conserved. Second, potent candidate
inhibitors against MtMurI were identified through docking against
already known inhibitors in other organisms.