Abstract: In order to investigate the prebiotic potential of
oligosaccharides prepared by chemical hydrolysis of water-soluble
polysaccharides (WSP) from Zizyphus lotus leaves, the effect of
oligosaccharides on bacterial growth was studied. The chemical
composition of WSP was evaluated by colorimetric assays revealed
the average values: 7.05±0.73% proteins and 86.21±0.74%
carbohydrates, among them 64.81±0.42% is neutral sugar and the rest
16.25±1.62% is uronic acids. The characterization of
monosaccharides was determined by high performance anion
exchange chromatography with pulsed amperometric detection
(HPAEC-PAD) was found to be composed of galactose (23.95%),
glucose (21.30%), rhamnose (20.28%), arabinose (9.55%), and
glucuronic acid (22.95%). The effects of oligosaccharides on the
growth of lactic acid bacteria were compared with those of fructooligosaccharide
(RP95). The oligosaccharides concentration was
1g/L of Man, Rogosa, Sharpe broth. Bacterial growth was assessed
during 2, 4.5, 6.5, 9, 12, 16 and 24 h by measuring the optical density
of the cultures at 600 nm (OD600) and pH values. During
fermentation, pH in broth cultures decreased from 6.7 to 5.87±0.15.
The enumeration of lactic acid bacteria indicated that
oligosaccharides led to a significant increase in bacteria (P≤0.05)
compared to the control. The fermentative metabolism appeared to be
faster on RP95 than on oligosaccharides from Zizyphus lotus leaves.
Both RP95 and oligosaccharides showed clear prebiotic effects, but
had differences in fermentation kinetics because of to the different
degree of polymerization. This study shows the prebiotic
effectiveness of oligosaccharides, and provides proof for the selection
of leaves of Zizyphus lotus for use as functional food ingredients.
Abstract: The aims of study were investigation on chemical
composition essential oil and the effect of extract of Coronilla varia
on antimicrobial and cytotoxicity activity. The essential oils of
Coronilla varia is obtained by hydrodistillation and analyzed by
(GC/MS) for determining their chemical composition and
identification of their components. Antibacterial activity of plant
extract was determined by disc diffusion method and anticancer
activity measured by MTT assay. The major components in essential
oil were Caryophyllene Oxide (60.19%), Alphacadinol (4.13%) and
Homoadantaneca Robexylic Acid (3.31%). The extracts from
Coronilla varia had interesting activity against Proteus mirabilis in
the concentration of 700 μg/disc and did not show any activity
against Staphylococus aureus, Bacillus subtillis, Klebsiella
pneumonia and Entrobacter cloacae. The positive control,
Ampicillin, Chloramphenicol and Cenphalothin had shown zone of
inhibition resistant all bacteria. The ethanol extract of Corohilla varia
inhibited on MCF7 cell lines. IC50 0.6(mg/ml) was the optimum
concentration of extract from Coronilla varia inhibition of cell line
growth. The MCF7 cancer cell line and Proteus mirabilis were more
sensitive to Coronilla varia ethanol extract.
Abstract: Crops diversity and maintaining and enhancing the
fertility of agricultural lands are basic principles of organic farming.
With a wider range of crops in agroecosystem can improve the ability
to control weeds, pests and diseases, and the performance of crops
rotation and food safety. In this sense, the main objective of the
research was to study the productivity and chemical composition of
some alternative crops and their adaptability to soil and climatic
conditions of the agricultural area in Southern Romania and to
cultivation in the organic farming system. The alternative crops were:
lentil (7 genotypes); five species of grain legumes (5 genotypes); four
species of oil crops (5 genotypes). The seed production was, on
average: 1343 kg/ha of lentil; 2500 kg/ha of field beans; 2400 kg/ha
of chick peas and blackeyed peas; more than 2000 kg/ha of atzuki
beans, over 1250 kg/ha of fenugreek; 2200 kg/ha of safflower; 570
kg/ha of oil pumpkin; 2150 kg/ha of oil flax; 1518 kg/ha of camelina.
Regarding chemical composition, lentil seeds contained: 22.18%
proteins, 3.03% lipids, 33.29% glucides, 4.00% minerals, and 259.97
kcal energy values. For field beans: 21.50% proteins, 4.40% lipids,
63.90% glucides, 5.85% minerals, 395.36 kcal energetic value. For
chick peas: 21.23% proteins, 4.55% lipids, 53.00% glucides, 3.67%
minerals, 348.22 kcal energetic value. For blackeyed peas: 23.30%
proteins, 2.10% lipids, 68.10% glucides, 3.93% minerals, 350.14 kcal
energetic value. For adzuki beans: 21.90% proteins, 2.60% lipids,
69.30% glucides, 4.10% minerals, 402.48 kcal energetic value. For
fenugreek: 21.30% proteins, 4.65% lipids, 63.83% glucides, 5.69%
minerals, 396.54 kcal energetic value. For safflower: 12.60%
proteins, 28.37% lipids, 46.41% glucides, 3.60% minerals, 505.78
kcal energetic value. For camelina: 20.29% proteins, 31.68% lipids,
36.28% glucides, 4.29% minerals, 526.63 kcal energetic value. For
oil pumpkin: 29.50% proteins, 36.92% lipids, 18.50% glucides,
5.41% minerals, 540.15 kcal energetic value. For oil flax: 22.56%
proteins, 34.10% lipids, 27.73% glucides, 5.25% minerals, 558.45
kcal energetic value.
Abstract: Themain goal of this article is to find efficient
methods for elemental and molecular analysis of living
microorganisms (algae) under defined environmental conditions and
cultivation processes. The overall knowledge of chemical
composition is obtained utilizing laser-based techniques, Laser-
Induced Breakdown Spectroscopy (LIBS) for acquiring information
about elemental composition and Raman Spectroscopy for gaining
molecular information, respectively. Algal cells were suspended in
liquid media and characterized using their spectra. Results obtained
employing LIBS and Raman Spectroscopy techniques will help to
elucidate algae biology (nutrition dynamics depending on cultivation
conditions) and to identify algal strains, which have the potential for
applications in metal-ion absorption (bioremediation) and biofuel
industry. Moreover, bioremediation can be readily combined with
production of 3rd generation biofuels. In order to use algae for
efficient fuel production, the optimal cultivation parameters have to
be determinedleading to high production of oil in selected
cellswithout significant inhibition of the photosynthetic activity and
the culture growth rate, e.g. it is necessary to distinguish conditions
for algal strain containing high amount of higher unsaturated fatty
acids. Measurements employing LIBS and Raman Spectroscopy were
utilized in order to give information about alga Trachydiscusminutus
with emphasis on the amount of the lipid content inside the algal cell
and the ability of algae to withdraw nutrients from its environment
and bioremediation (elemental composition), respectively. This
article can serve as the reference for further efforts in describing
complete chemical composition of algal samples employing laserablation
techniques.
Abstract: Bacterial magnetic nanoparticles have great useful potential in biotechnological and biomedical applications. In this study, a liquid growth medium was modified for cultivation a fastidious magnetotactic bacterium that has been isolated from Anzali
lagoon, Iran in our previous research. These modifications include
change in vitamin, mineral, carbon sources and etcetera. In our
experience, the serum bottles and designed air-tight laboratory bottles
were used to create microaerobic conditions in order to development
of a method for scale-up experiment. This information may serve as a
guide to green chemistry based biological protocols for the synthesis
of magnetic nanoparticles with control over the chemical
composition, morphology and size.