Abstract: In present scenario, cardiovascular problems are growing challenge for researchers and physiologists. As heart disease have no geographic, gender or socioeconomic specific reasons; detecting cardiac irregularities at early stage followed by quick and correct treatment is very important. Electrocardiogram is the finest tool for continuous monitoring of heart activity. Heart rate variability (HRV) is used to measure naturally occurring oscillations between consecutive cardiac cycles. Analysis of this variability is carried out using time domain, frequency domain and non-linear parameters. This paper presents HRV analysis of the online dataset for normal sinus rhythm (taken as healthy subject) and sudden cardiac death (SCD subject) using all three methods computing values for parameters like standard deviation of node to node intervals (SDNN), square root of mean of the sequences of difference between adjacent RR intervals (RMSSD), mean of R to R intervals (mean RR) in time domain, very low-frequency (VLF), low-frequency (LF), high frequency (HF) and ratio of low to high frequency (LF/HF ratio) in frequency domain and Poincare plot for non linear analysis. To differentiate HRV of healthy subject from subject died with SCD, k –nearest neighbor (k-NN) classifier has been used because of its high accuracy. Results show highly reduced values for all stated parameters for SCD subjects as compared to healthy ones. As the dataset used for SCD patients is recording of their ECG signal one hour prior to their death, it is therefore, verified with an accuracy of 95% that proposed algorithm can identify mortality risk of a patient one hour before its death. The identification of a patient’s mortality risk at such an early stage may prevent him/her meeting sudden death if in-time and right treatment is given by the doctor.
Abstract: Present project consists in a study and a development
of piezoelectric devices for supplying power to new generation
pacemakers. They are miniaturized leadless implants without battery
placed directly in right ventricle. Amongst different acceptable
energy sources in cardiac environment, we choose the solution of a
device based on conversion of the energy produced by pressure
variation inside the heart into electrical energy. The proposed energy
harvesters can meet the power requirements of pacemakers, and can
be a good solution to solve the problem of regular surgical operation.
With further development, proposed device should provide enough
energy to allow pacemakers autonomy, and could be good candidate
for next pacemaker generation.
Abstract: Segmentation of left ventricle (LV) from cardiac
ultrasound images provides a quantitative functional analysis of the
heart to diagnose disease. Active Shape Model (ASM) is widely used
for LV segmentation, but it suffers from the drawback that
initialization of the shape model is not sufficiently close to the target,
especially when dealing with abnormal shapes in disease. In this work,
a two-step framework is improved to achieve a fast and efficient LV
segmentation. First, a robust and efficient detection based on Hough
forest localizes cardiac feature points. Such feature points are used to
predict the initial fitting of the LV shape model. Second, ASM is
applied to further fit the LV shape model to the cardiac ultrasound
image. With the robust initialization, ASM is able to achieve more
accurate segmentation. The performance of the proposed method is
evaluated on a dataset of 810 cardiac ultrasound images that are mostly
abnormal shapes. This proposed method is compared with several
combinations of ASM and existing initialization methods. Our
experiment results demonstrate that accuracy of the proposed method
for feature point detection for initialization was 40% higher than the
existing methods. Moreover, the proposed method significantly
reduces the number of necessary ASM fitting loops and thus speeds up
the whole segmentation process. Therefore, the proposed method is
able to achieve more accurate and efficient segmentation results and is
applicable to unusual shapes of heart with cardiac diseases, such as left
atrial enlargement.
Abstract: The aim of this paper is to propose a general
framework for storing, analyzing, and extracting knowledge from
two-dimensional echocardiographic images, color Doppler images,
non-medical images, and general data sets. A number of high
performance data mining algorithms have been used to carry out this
task. Our framework encompasses four layers namely physical
storage, object identification, knowledge discovery, user level.
Techniques such as active contour model to identify the cardiac
chambers, pixel classification to segment the color Doppler echo
image, universal model for image retrieval, Bayesian method for
classification, parallel algorithms for image segmentation, etc., were
employed. Using the feature vector database that have been
efficiently constructed, one can perform various data mining tasks
like clustering, classification, etc. with efficient algorithms along
with image mining given a query image. All these facilities are
included in the framework that is supported by state-of-the-art user
interface (UI). The algorithms were tested with actual patient data
and Coral image database and the results show that their performance
is better than the results reported already.
Abstract: The myocardium is composed of specialized muscle
which relies mainly on fatty acid and sugar metabolism and it is
widely contribute to the heart functioning. The changes of the cardiac
energy-producing system during heart failure have been proved using
autoradiography techniques. This study focused on evaluating sugar
and fatty acid metabolism in myocardium as cardiac energy getting
system using heart-accumulated radiopharmaceuticals. Two sets of
autoradiographs of heart cross sections of Lewis male rats were
analyzed and the time- accumulation curve obtained with use of the
MATLAB image processing software to evaluate fatty acid and sugar
metabolic functions.
Abstract: It has been known that a characteristic
Burst-Suppression (BS) pattern appears in EEG during the early
recovery period following Cardiac Arrest (CA). Here, to explore the
relationship between cortical and subcortical neural activities
underlying BS, extracellular activity in the parietal cortex and the
centromedian nucleus of the thalamus and extradural EEG were
recorded in a rodent CA model. During the BS, the cortical firing rate
is extraordinarily high, and that bursts in EEG correlate to dense spikes
in cortical neurons. Newly observed phenomena are that 1) thalamic
activity reemerges earlier than cortical activity following CA, and 2)
the correlation coefficient of cortical and thalamic activities rises
during BS period. These results would help elucidate the underlying
mechanism of brain recovery after CA injury.
Abstract: Phonocardiography is important in appraisal of
congenital heart disease and pulmonary hypertension as it reflects the
duration of right ventricular systoles. The systolic murmur in patients
with intra-cardiac shunt decreases as pulmonary hypertension
develops and may eventually disappear completely as the pulmonary
pressure reaches systemic level. Phonocardiography and auscultation
are non-invasive, low-cost, and accurate methods to assess heart
disease. In this work an objective signal processing tool to extract
information from phonocardiography signal using Wavelet is
proposed to classify the murmur as normal or abnormal. Since the
feature vector is large, a Binary Particle Swarm Optimization (PSO)
with mutation for feature selection is proposed. The extracted
features improve the classification accuracy and were tested across
various classifiers including Naïve Bayes, kNN, C4.5, and SVM.
Abstract: The effect of processing on plasma lipid profile and
atherogenic indices of rats fed Artocarpus heterophyllus seed diets at
different concentrations were investigated. Fifty five rats were used
for this study, they were divided into eleven groups of five rats each
(one control group and ten test groups), the test groups were fed raw,
boiled, roasted, fermented and soaked diets at 10% and 40%
concentrations. The study lasted for thirty five days. The diets led to
significant decrease (p
Abstract: The paper presents a novel screening method to
indicate congenital heart diseases (CHD), which otherwise could
remain undetected because of their low level. Therefore, not
belonging to the high-risk population, the pregnancies are not subject
to the regular fetal monitoring with ultrasound echocardiography.
Based on the fact that CHD is a morphological defect of the heart
causing turbulent blood flow, the turbulence appears as a murmur,
which can be detected by fetal phonocardiography (fPCG). The
proposed method applies measurements on the maternal abdomen
and from the recorded sound signal a sophisticated processing
determines the fetal heart murmur. The paper describes the problems
and the additional advantages of the fPCG method including the
possibility of measurements at home and its combination with the
prescribed regular cardiotocographic (CTG) monitoring. The
proposed screening process implemented on a telemedicine system
provides an enhanced safety against hidden cardiac diseases.
Abstract: This work proposes a data-driven multiscale based
quantitative measures to reveal the underlying complexity of
electroencephalogram (EEG), applying to a rodent model of
hypoxic-ischemic brain injury and recovery. Motivated by that real
EEG recording is nonlinear and non-stationary over different
frequencies or scales, there is a need of more suitable approach over
the conventional single scale based tools for analyzing the EEG data.
Here, we present a new framework of complexity measures
considering changing dynamics over multiple oscillatory scales. The
proposed multiscale complexity is obtained by calculating entropies of
the probability distributions of the intrinsic mode functions extracted
by the empirical mode decomposition (EMD) of EEG. To quantify
EEG recording of a rat model of hypoxic-ischemic brain injury
following cardiac arrest, the multiscale version of Tsallis entropy is
examined. To validate the proposed complexity measure, actual EEG
recordings from rats (n=9) experiencing 7 min cardiac arrest followed
by resuscitation were analyzed. Experimental results demonstrate that
the use of the multiscale Tsallis entropy leads to better discrimination
of the injury levels and improved correlations with the neurological
deficit evaluation after 72 hours after cardiac arrest, thus suggesting an
effective metric as a prognostic tool.
Abstract: The number of persons with implanted cardiac
pacemakers (PM) has increased in Western countries. The aim of this
paper is to investigate the possible situations where persons with a
PM may be exposed to extremely low frequency (ELF) electric (EF)
and magnetic fields (MF) that may disturb their PM. Based on our
earlier studies, it is possible to find such high public exposure to EFs
only in some places near 400 kV power lines, where an EF may
disturb a PM in unipolar mode. Such EFs cannot be found near 110
kV power lines. Disturbing MFs can be found near welding
machines. However, we do not have measurement data from welding.
Based on literature and earlier studies at Tampere University of
Technology, it is difficult to find public EF or MF exposure that is
high enough to interfere with PMs.
Abstract: Advances in the use of health care technology have
resulted in increased adverse events (AEs) related to the use of
medical devices. The study focused on the existing reporting systems.
This study was conducted in a tertiary care public sector hospital.
Devices included Syringe infusion pumps, Cardiac monitors, Pulse
oximeters, Ventilators and Defibrillators. A total of 211 respondents
were recruited. Interviews were held with 30 key informants. Medical
records were scrutinized. Relevant statistical tests were used.
Resident doctors reported maximum frequency of AEs, followed
by nurses; and least by consultants. A significant association was
found between the cadre of health care personnel and awareness that
the patients and bystanders have a risk of sustaining AE. Awareness
regarding reporting of AEs was low, and it was generally done
verbally. Other critical findings are discussed in the light of the
barriers to reporting, reasons for non-compliance, recording system,
and so on.
Abstract: Background: The objectives of this study were to
assess patient’s knowledge of appropriate sublingual glyceryl
trinitrate (GTN) use as well as to investigate how patients commonly
store and carry their sublingual GTN tablets. Methodology: This was
a cross-sectional survey, using a validated researcher-administered
questionnaire. The study involved cardiac patients receiving
sublingual GTN attending the outpatient and inpatient departments of
Taiping Hospital, a non-academic public care hospital. The minimum
calculated sample size was 92, but 100 patients were conveniently
sampled. Respondents were interviewed on 3 areas, including
demographic data, knowledge and use of sublingual GTN. Eight
items were used to calculate each subject’s knowledge score and six
items were used to calculate use score. Results: Of the 96 patients
who consented to participate, majority (96.9%) were well aware of
the indication of sublingual GTN. With regards to the mechanism of
action of sublingual GTN, 73 (76%) patients did not know how the
medication works. Majority of the patients (66.7%) knew about the
proper storage of the tablet. In relation to the maximum number of
sublingual GTN tablets that can be taken during each angina episode,
36.5% did not know that up to 3 tablets of sublingual GTN can be
taken during each episode of angina. Fifty four (56.2%) patients were
not aware that they need to replace sublingual GTN every 8 weeks
after receiving the tablets. Majority (69.8%) of the patients
demonstrated lack of knowledge with regards to the use of sublingual
GTN as prevention of chest pain. Conclusion: Overall, patients’
knowledge regarding the self-administration of sublingual GTN is
still inadequate. The findings support the need for more frequent
reinforcement of patient education, especially in the areas of
preventive use, storage and drug stability.
Abstract: Quantification of cardiac function is performed by
calculating blood volume and ejection fraction in routine clinical
practice. However, these works have been performed by manual
contouring, which requires computational costs and varies on the
observer. In this paper, an automatic left ventricle segmentation
algorithm on cardiac magnetic resonance images (MRI) is presented.
Using knowledge on cardiac MRI, a K-mean clustering technique is
applied to segment blood region on a coil-sensitivity corrected image.
Then, a graph searching technique is used to correct segmentation
errors from coil distortion and noises. Finally, blood volume and
ejection fraction are calculated. Using cardiac MRI from 15 subjects,
the presented algorithm is tested and compared with manual
contouring by experts to show outstanding performance.
Abstract: VACTERL association is a rare disorder with various
congenital malformations. The aetiology remains unknown.
Combination of at least three congenital anomalies of the following
criteria is required for diagnosis: vertebral defects, anal atresia,
cardiac anomalies, tracheo-esophageal fistula, renal anomalies, and
limb defects. The first case was 1-day old male neonate with multiple
congenital anomalies was bore from 28 years old mother. The mother
had history of pregnancy with lymphocyte therapy. His anomalies
included: defects in thoracic and lumbar vertebral, anal atresia,
bilateral hydronephrosis, atrial septal defect, and lower limb
abnormality. Other anomalies were cryptorchidism and nasal canal
narrowing. The second case was born with 32 weeks gestational age
from mother with history of pregnancy with lymphocyte therapy. He
had thoracic vertebral defect, cardiac anomalies and renal defect.
diagnosis based on clinical finding is VACTERL association. Early
diagnosis is very important to investigation and treatment of other
coexistence anomalies. VACTERL association in mothers with
history of pregnancy with lymphocyte therapy has suggested possibly
of relationship between VACTERL association and this method of
pregnancy.
Abstract: Liposome plays an important role in medical and
pharmaceutical science as e.g. nano scale drug carriers. Liposomes
are vesicles of varying size consisting of a spherical lipid bilayer and
an aqueous inner compartment. Magnet-driven liposome used for the
targeted delivery of drugs to organs and tissues. These liposome
preparations contain encapsulated drug components and finely
dispersed magnetic particles.
Liposomes are vesicles of varying size consisting of a spherical
lipid bilayer and an aqueous inner compartment that are generated in
vitro. These are useful in terms of biocompatibility, biodegradability,
and low toxicity, and can control biodistribution by changing the size,
lipid composition, and physical characteristics. Furthermore,
liposomes can entrap both hydrophobic and hydrophilic drugs and are
able to continuously release the entrapped substrate, thus being useful
drug carriers. Magnetic liposomes (MLs) are phospholipid vesicles
that encapsulate magneticor paramagnetic nanoparticles. They are
applied as contrast agents for magnetic resonance imaging (MRI).
The biological synthesis of nanoparticles using plant extracts plays
an important role in the field of nanotechnology. Green-synthesized
magnetite nanoparticles-protein hybrid has been produced by treating
Iron (III) / Iron (II) chloride with the leaf extract of Datura inoxia.
The phytochemicals present in the leaf extracts act as a reducing as
well stabilizing agents preventing agglomeration, which include
flavonoids, phenolic compounds, cardiac glycosides, proteins and
sugars.
The magnetite nanoparticles-protein hybrid has been trapped
inside the aqueous core of the liposome prepared by reversed phase
evaporation (REV) method using oleic and linoleic acid which has
been shown to be driven under magnetic field confirming the
formation magnetic liposome (ML). Chemical characterization of
stealth magnetic liposome has been performed by breaking the
liposome and release of magnetic nanoparticles. The presence iron
has been confirmed by colour complex formation with KSCN and
UV-Vis study using spectrophotometer Cary 60, Agilent.
This magnet driven liposome using nanoparticles-protein hybrid
can be a smart vesicles for the targeted drug delivery.
Abstract: To evaluate the factors which predetermine the
coronary artery disease in patients having positive Exercise Tolerance
Test (ETT) that is treadmill results and coronary artery findings. This
descriptive study was conducted at Department of Cardiology,
Ibrahim Cardiac Hospital & Research Institute, Dhaka, Bangladesh
from 1st January, 2014 to 31st August, 2014. All patients who had
done ETT (treadmill) for chest pain diagnosis were studied. One
hundred and four patients underwent coronary angiogram after
positive treadmill result. Patients were divided into two groups
depending upon the angiographic findings, i.e. true positive and false
positive. Positive treadmill test patients who have coronary artery
involvement these are called true positive and who have no
involvement they are called false positive group. Both groups were
compared with each other. Out of 104 patients, 81 (77.9%) patients
had true positive ETT and 23 (22.1%) patients had false positive
ETT. The mean age of patients in positive ETT was 53.46± 8.06
years and male mean age was 53.63±8.36 years and female was
52.87±7.0 years. Sixty nine (85.19%) male patients and twelve
(14.81%) female patients had true positive ETT, whereas 15
(65.21%) males and 8 (34.79%) females had false positive ETT, this
was statistically significant (p
Abstract: Since the marine environmental conditions are
extremely different from the other ones, marine actinomycetes might
produce novel bioactive compounds. Therefore, actinomycete strains
were screened from marine water and sediment samples collected
from the coastal areas of Northern Vietnam. Ninety-nine
actinomycete strains were obtained on starch-casein agar media by
dilution technique, only seven strains, named HP112, HP12, HP411,
HPN11, HP 11, HPT13 and HPX12, showed significant antibacterial
activity against both gram-positive and gram-negative bacteria
(Bacillus subtilis ATCC 6633, Staphylococcus epidemidis ATCC
12228, Escherichia coli ATCC 11105). Further studies were carried
out with the most active HP411 strain against Candida albicans
ATCC 10231. This strain could grow rapidly on starch casein agar
and other media with high salt containing 7-10% NaCl at 28-30oC.
Spore-chain of HP411 showed an elongated and circular shape with
10 to 30 spores/chain. Identification of the strain was carried out by
employing the taxonomical studies including the 16S rRNA
sequence. Based on phylogenetic and phenotypic evidence it is
proposed that HP411 to be belongs to species Streptomyces
variabilis. The potent of the crude extract of fermentation broth of
HP411 that are effective against wide range of pathogens: both grampositive,
gram-negative and fungi. Further studies revealed that the
crude extract HP411 could obtain the anticancer activity for cancer
cell lines: Hep-G2 (liver cancer cell line); RD (cardiac and skeletal
muscle letters cell line); FL (membrane of the uterus cancer cell line).
However, the actinomycetes from marine ecosystem will be useful
for the discovery of new drugs in the future.
Abstract: Regular exercise promotes reduction in blood pressure, reduction in body weight and it also helps to increase in insulin sensitivity. Participation in physical activity should always be linked to medical screening which can reveal serious medical problems. One of them is high blood pressure. Hypertension is risk factor for one billion people worldwide and the highest prevalence is found in Africa. Another component of hypertension is that people who suffer from hypertension have no symptoms. It is estimated that reduction of 3mm Hg in Systolic Blood Pressure decreases cardiac morbidity at least 5%. The most of the guidelines suggest aerobic exercise in a prevention of cardiovascular diseases. On the other hand, it is important to emphasize the impact of resistance training. Even, it was found higher effect for reduction on the level of systolic blood pressure than aerobic exercise.
Abstract: An accurate study of blood flow is associated with an accurate vascular pattern and geometrical properties of the organ of interest. Due to the complexity of vascular networks and poor accessibility in vivo, it is challenging to reconstruct the entire vasculature of any organ experimentally. The objective of this study is to introduce an innovative approach for the reconstruction of a full vascular tree from available morphometric data. Our method consists of implementing morphometric data on those parts of the vascular tree that are smaller than the resolution of medical imaging methods. This technique reconstructs the entire arterial tree down to the capillaries. Vessels greater than 2 mm are obtained from direct volume and surface analysis using contrast enhanced computed tomography (CT). Vessels smaller than 2mm are reconstructed from available morphometric and distensibility data and rearranged by applying Murray’s Laws. Implementation of morphometric data to reconstruct the branching pattern and applying Murray’s Laws to every vessel bifurcation simultaneously, lead to an accurate vascular tree reconstruction. The reconstruction algorithm generates full arterial tree topography down to the first capillary bifurcation. Geometry of each order of the vascular tree is generated separately to minimize the construction and simulation time. The node-to-node connectivity along with the diameter and length of every vessel segment is established and order numbers, according to the diameter-defined Strahler system, are assigned. During the simulation, we used the averaged flow rate for each order to predict the pressure drop and once the pressure drop is predicted, the flow rate is corrected to match the computed pressure drop for each vessel. The final results for 3 cardiac cycles is presented and compared to the clinical data.