Nanoparticles-Protein Hybrid Based Magnetic Liposome

Liposome plays an important role in medical and
pharmaceutical science as e.g. nano scale drug carriers. Liposomes
are vesicles of varying size consisting of a spherical lipid bilayer and
an aqueous inner compartment. Magnet-driven liposome used for the
targeted delivery of drugs to organs and tissues. These liposome
preparations contain encapsulated drug components and finely
dispersed magnetic particles.
Liposomes are vesicles of varying size consisting of a spherical
lipid bilayer and an aqueous inner compartment that are generated in
vitro. These are useful in terms of biocompatibility, biodegradability,
and low toxicity, and can control biodistribution by changing the size,
lipid composition, and physical characteristics. Furthermore,
liposomes can entrap both hydrophobic and hydrophilic drugs and are
able to continuously release the entrapped substrate, thus being useful
drug carriers. Magnetic liposomes (MLs) are phospholipid vesicles
that encapsulate magneticor paramagnetic nanoparticles. They are
applied as contrast agents for magnetic resonance imaging (MRI).
The biological synthesis of nanoparticles using plant extracts plays
an important role in the field of nanotechnology. Green-synthesized
magnetite nanoparticles-protein hybrid has been produced by treating
Iron (III) / Iron (II) chloride with the leaf extract of Datura inoxia.
The phytochemicals present in the leaf extracts act as a reducing as
well stabilizing agents preventing agglomeration, which include
flavonoids, phenolic compounds, cardiac glycosides, proteins and
sugars.
The magnetite nanoparticles-protein hybrid has been trapped
inside the aqueous core of the liposome prepared by reversed phase
evaporation (REV) method using oleic and linoleic acid which has
been shown to be driven under magnetic field confirming the
formation magnetic liposome (ML). Chemical characterization of
stealth magnetic liposome has been performed by breaking the
liposome and release of magnetic nanoparticles. The presence iron
has been confirmed by colour complex formation with KSCN and
UV-Vis study using spectrophotometer Cary 60, Agilent.
This magnet driven liposome using nanoparticles-protein hybrid
can be a smart vesicles for the targeted drug delivery.

• Amlan Kumar Das
• Avinash Marwal
• Vikram Pareek

• Nanoparticles-Protein Hybrid
• Magnetic Liposome.

[1] LZhang , J M Chan, F XGu, J WRhee, A Z Wang, A F Radovic-Moreno, F Alexis, R Langerand O C Farokhzad “ Self-Assembled Lipid Polymer Hybrid Nanoparticles- A Robust Drug Delivery Platform”, ACS Nan, 2, 2008, pp. 1696 – 1702.
[2] DFrascione, C Diwoky,G Almer, P Opriessing, C Vonach, KGradauer, G Leitinger, H Mangge, R Stollbergerand R Prassl “Ultrasmallsuperparamagnetic iron oxide (USPIO)-based liposomes as magnetic resonance imaging probe”International Journal of Nanomedicine, 7,2012, pp. 2349–2359.
[3] G KIsmailova,V IEfremenkoandA G Kuregyan, “ Biotechnology of Magnet-Driven Liposome Preparations.” , Pharmaceutical Chemistry Journal, 39,2005, pp. 47 – 49.
[4] P Pradhan,J Giri,R Banerjee ,JBellare J and D Bahadur D “ Preparation and characterization of manganese ferrite-based magnetic liposomes for hyperthermia treatment of cancer.”,Journal of Magnetism and Magnetic Materials, 311, 2007, pp. 208–215.
[5] A M Awwadand N M Salem “A Green and Facile Approach for Synthesis of Magnetite Nanoparticles”, Nanoscience and Nanotechnology, 2, 2012, pp. 208-213.
[6] M Mahdavi, F Namvar, M B Ahmad and R Mohamad R, “Green Biosynthesis and Characterization of Magnetic Iron Oxide (Fe3O4) Nanoparticles Using Seaweed (Sargassummuticum) Aqueous Extract” Molecules, 18, 2013, pp. 5954-5964.
[7] M Pattanayakand P L Nayak “Ecofriendly green synthesis of iron nanoparticles from various plants and spices extract” International Journal of Plant, Animal and Environmental Science, 3, 2013, pp. 68-78.
[8] Amlan Kumar Das, Avinashand RuchiVerma“ Bio-reductive synthesis and characterization of plant protein coated magnetite nanoparticles” Nano Hybrid, Trans Tech Publications, 7, 2014, pp. 69-86
[9] Amlan Kumar Das, AvinashMarwal and RuchiVerma “Nanotechnology: Novel Perspectives and Prospects” McGraw Hill, USA, 2014, pp. 136-143
[10] M MYallapu, S P Foy, T K Jain and V Labhasetwar, “ PEG-Functionalized Magnetic Nanoparticles for Drug Delivery and Magnetic Resonance Imaging Applications” Pharm Res. 27, 201, pp. 2283–2295.
[11] Manager C and Cabul V: Synthesis of magnetic liposomes: Journal of colloid and interface science, 169, 1995, pp. 251 – 253.
[12] M MElmi and M NSarbolouki,“A simple method for preparation of immuno-magnetic liposomes” ,International Journal of Pharmaceutics, 215, 2001, pp. 45–50.
[13] Amlan Kumar Das, AvinashMarwal and RuchiVerma “Preparation and Characterization of Nano-bio hybrid based Magnetoliposomes” International Journal of Pharmaceutical sciences and Research, 6, 2015, pp. 367-375.