An AFM Approach of RBC Micro and Nanoscale Topographic Features during Storage

Blood gamma irradiation is the only available method to prevent transfusion associated graft versus host disease (TAGVHD). However, when blood is irradiated, determine blood shelf time is crucial. Non irradiated blood have a self-time from 21 to 35 days when is preserved with anticoagulated solution and stored at 4°C. During their storage, red blood cells (RBC) undergo a series of biochemical, biomechanical and molecular changes involving what is known as storage lesion (SL). SL include loss of structural integrity of RBC, decrease of 2,3-diphosphatidylglyceric acid levels, and increase of both ion potassium concentration and hemoglobin (Hb). On the other hand, Atomic force Microscopy (AFM) represents a versatile tool for a nano-scale high resolution topographic analysis in biological systems. In order to evaluate SL in irradiated and nonirradiated blood, RBC topography and morphometric parameters were obtained from an AFM XE-BIO system. Cell viability was followed using flow cytometry. Our results showed that early markers as nanoscale roughness, allow us to evaluate blood quality since other perspective.

A Study on the Iterative Scheme for Stratified Shields Gamma Ray Buildup FactorsUsing Layer-Splitting Technique in Double-Layer Shields

Theiterative scheme which is used to treat buildup factors for stratified shields is being investigated here using the layer-splitting technique.A simple suggested formalism for the scheme based on the Kalos’ formula is introduced, based on which the implementation of the testing technique is carried out. The second layer in a double-layer shield was split into two equivalent layers and the scheme (with the suggested formalism) was implemented on the new “three-layer” shieldconfiguration.The results of such manipulation on water-lead and water-iron shields combinations are presented here for 1MeV photons. It was found that splitting the second layer introduces some deviation on the overall buildup factor value. This expected deviation appeared to be higher in the case of low Z layer followed by high Z. However, the overall performance of the iterative scheme showed a great consistency and strong coherence even with the introduced changes. The introduced layer-splitting testing technique shows the capability to be implemented in test the iterative scheme with a wide range of formalisms.

Gamma Glutamyl Transferase and Lactate Dehydrogenase as Biochemical Markers of Severity of Preeclampsia

This study was conducted to examine the possible role of serum Gamma-glutamyltransferase (GGT) and Lactate dehydrogenase (LDH) in the prediction of severity of preeclampsia. The study group comprised of 40 preeclamptic cases (22 with mild and 18 with severe) and 40 healthy normotensive pregnant controls. Serum samples of all the cases were assayed for GGT and LDH. Demographic, hemodynamic and laboratory data as well as serum GGT and LDH levels were compared among the three groups. The results indicated that severe preeclamptic cases had significantly increased levels of serum GGT and LDH. The symptoms in severe preeclamptic women were significantly increased in patients with GGT > 70 IU/L and LDH >800 IU/L. Elevated levels of serum GGT and LDH can be used as biochemical markers which reflects the severity of preeclampsia and useful for the management of preeclampsia to decrease maternal and fetal morbidity and mortality.

Enhanced Approaches to Rectify the Noise, Illumination and Shadow Artifacts

Enhancing the quality of two dimensional signals is one of the most important factors in the fields of video surveillance and computer vision. Usually in real-life video surveillance, false detection occurs due to the presence of random noise, illumination and shadow artifacts. The detection methods based on background subtraction faces several problems in accurately detecting objects in realistic environments: In this paper, we propose a noise removal algorithm using neighborhood comparison method with thresholding. The illumination variations correction is done in the detected foreground objects by using an amalgamation of techniques like homomorphic decomposition, curvelet transformation and gamma adjustment operator. Shadow is removed using chromaticity estimator with local relation estimator. Results are compared with the existing methods and prove as high robustness in the video surveillance.

Investigation on Nanoparticle Velocity in Two Phase Approach

Numerical investigation on the generality of nanoparticle velocity equation had been done on the previous published work. The three dimensional governing equations (continuity, momentum and energy) were solved using finite volume method (FVM). Parametric study of thermal performance between pure water-cooled and nanofluid-cooled are evaluated for volume fraction in the range of 1% to 4%, and nanofluid type of gamma-Al2O3 at Reynolds number range of 67.41 to 286.77. The nanofluid is modeled using single and two phase approach. Three different existing Brownian motion velocities are applied in comparing the generality of the equation for a wide parametric condition. Deviation in between the Brownian motion velocity is identified to be due to the different means of mean free path and constant value used in diffusion equation.

Robust Coherent Noise Suppression by Point Estimation of the Cauchy Location Parameter

This paper introduces a new point estimation algorithm, with particular focus on coherent noise suppression, given several measurements of the device under test where it is assumed that 1) the noise is first-order stationery and 2) the device under test is linear and time-invariant. The algorithm exploits the robustness of the Pitman estimator of the Cauchy location parameter through the initial scaling of the test signal by a centred Gaussian variable of predetermined variance. It is illustrated through mathematical derivations and simulation results that the proposed algorithm is more accurate and consistently robust to outliers for different tailed density functions than the conventional methods of sample mean (coherent averaging technique) and sample median search.

Gene Network Analysis of PPAR-γ: A Bioinformatics Approach Using STRING

Gene networks present a graphical view at the level of gene activities and genetic functions and help us to understand complex interactions in a meaningful manner. In the present study, we have analyzed the gene interaction of PPAR-γ (peroxisome proliferator-activated receptor gamma) by search tool for retrieval of interacting genes. We find PPAR-γ is highly networked by genetic interactions with 10 genes: RXRA (retinoid X receptor, alpha), PPARGC1A (peroxisome proliferator-activated receptor gamma, coactivator 1 alpha), NCOA1 (nuclear receptor coactivator 1), NR0B2 (nuclear receptor subfamily 0, group B, member 2), HDAC3 (histone deacetylase 3), MED1 (mediator complex subunit 1), INS (insulin), NCOR2 (nuclear receptor co-repressor 2), PAX8 (paired box 8), ADIPOQ (adiponectin) and it augurs well for the fact that obesity and several other metabolic disorders are inter related.

Phase Transition Characteristics of Flame-Synthesized Gamma-Al2O3 Nanoparticles with Heat Treatment

In this study, the phase transition characteristics of flame-synthesized γ-Al2O3 nanoparticles to α-Al2O3 have been investigated. The nanoparticles were synthesized by using a coflow hydrogen diffusion flame. The phase transition and particle characteristics of the Al2O3 nanoparticles were determined by examining the crystalline structure and the shape of the collected nanoparticles before and after the heat treatment. The morphology and crystal structure of the Al2O3 nanoparticles were determined from SEM images and XRD analyses, respectively. The measured specific surface area and averaged particle size were 63.44m2/g and 23.94nm, respectively. Based on the scanning electron microscope images and x-ray diffraction patterns, it is believed that the onset temperature of the phase transition to α-Al2O3 was existed near 1200oC. The averaged diameters of the sintered particles heat treated at 1,260oC were approximately 80nm.

Profile Calculation in Water Phantom of Symmetric and Asymmetric Photon Beam

Nowadays, in most radiotherapy departments, the commercial treatment planning systems (TPS) used to calculate dose distributions needs to be verified; therefore, quick, easy-to-use and low cost dose distribution algorithms are desirable to test and verify the performance of the TPS. In this paper, we put forth an analytical method to calculate the phantom scatter contribution and depth dose on the central axis based on the equivalent square concept. Then, this method was generalized to calculate the profiles at any depth and for several field shapes regular or irregular fields under symmetry and asymmetry photon beam conditions. Varian 2100 C/D and Siemens Primus Plus Linacs with 6 and 18 MV photon beam were used for irradiations. Percentage depth doses (PDDs) were measured for a large number of square fields for both energies, and for 45º wedges which were employed to obtain the profiles in any depth. To assess the accuracy of the calculated profiles, several profile measurements were carried out for some treatment fields. The calculated and measured profiles were compared by gamma-index calculation. All γ–index calculations were based on a 3% dose criterion and a 3 mm dose-to-agreement (DTA) acceptance criterion. The γ values were less than 1 at most points. However, the maximum γ observed was about 1.10 in the penumbra region in most fields and in the central area for the asymmetric fields. This analytical approach provides a generally quick and fairly accurate algorithm to calculate dose distribution for some treatment fields in conventional radiotherapy.

Thyroids Dose Evaluation and Calculation of Backscatter Factors for Co-60 Irradiations

The aim of the study is evaluation of absorbed doses for thyroids by using neck phantoms. For this purpose, it was arranged the irradiation set with different phantoms. Three different materials were used for phantom materials as, water, parafine and wood. The phantoms were three different dimensions for simulation of different ages and human race for each material. Co-60 gammao source was used for irradiation and the experimental procedure applied rigorously with narrow beam geometry.  As the results of the experiments the relative radiation doses are evaluated for therapic applications for thyroids and backscattering factors were calculated and shown that water, parafine and wood can appropriate for phantom material with the converge values of backscattering factors.

Statistical Description of Wave Interactions in 1D Defect Turbulence

We have investigated statistical properties of the defect turbulence in 1D CGLE wherein many body interaction is involved between local depressing wave (LDW) and local standing wave (LSW). It is shown that the counting number fluctuation of LDW is subject to the sub-Poisson statistics (SUBP). The physical origin of the SUBP can be ascribed to pair extinction of LDWs based on the master equation approach. It is also shown that the probability density function (pdf) of inter-LDW distance can be identified by the hyper gamma distribution. Assuming a superstatistics of the exponential distribution (Poisson configuration), a plausible explanation is given. It is shown further that the pdf of amplitude of LDW has a fattail. The underlying mechanism of its fluctuation is examined by introducing a generalized fractional Poisson configuration.

Improved Automated Classification of Alcoholics and Non-alcoholics

In this paper, several improvements are proposed to previous work of automated classification of alcoholics and nonalcoholics. In the previous paper, multiplayer-perceptron neural network classifying energy of gamma band Visual Evoked Potential (VEP) signals gave the best classification performance using 800 VEP signals from 10 alcoholics and 10 non-alcoholics. Here, the dataset is extended to include 3560 VEP signals from 102 subjects: 62 alcoholics and 40 non-alcoholics. Three modifications are introduced to improve the classification performance: i) increasing the gamma band spectral range by increasing the pass-band width of the used filter ii) the use of Multiple Signal Classification algorithm to obtain the power of the dominant frequency in gamma band VEP signals as features and iii) the use of the simple but effective knearest neighbour classifier. To validate that these two modifications do give improved performance, a 10-fold cross validation classification (CVC) scheme is used. Repeat experiments of the previously used methodology for the extended dataset are performed here and improvement from 94.49% to 98.71% in maximum averaged CVC accuracy is obtained using the modifications. This latest results show that VEP based classification of alcoholics is worth exploring further for system development.

Using Exponential Lévy Models to Study Implied Volatility patterns for Electricity Options

German electricity European options on futures using Lévy processes for the underlying asset are examined. Implied volatility evolution, under each of the considered models, is discussed after calibrating for the Merton jump diffusion (MJD), variance gamma (VG), normal inverse Gaussian (NIG), Carr, Geman, Madan and Yor (CGMY) and the Black and Scholes (B&S) model. Implied volatility is examined for the entire sample period, revealing some curious features about market evolution, where data fitting performances of the five models are compared. It is shown that variance gamma processes provide relatively better results and that implied volatility shows significant differences through time, having increasingly evolved. Volatility changes for changed uncertainty, or else, increasing futures prices and there is evidence for the need to account for seasonality when modelling both electricity spot/futures prices and volatility.

Coherent and Incoherent Scattering Cross Sections for Elements with 13

Coherent and incoherent scattering cross section measurements have been carried out using a HPGe detector on elements in the range of Z = 13 - 50 using 241Am gamma rays. The cross sections have been derived by comparing the net count rate obtained from the Compton peak of aluminium with the corresponding peak of the target. The measured cross sections for the coherent and incoherent processes are compared with theoretical values and earlier reported values. Our results are in agreement with the theoretical values.

Compton Scattering of Annihilation Photons as a Short Range Quantum Key Distribution Mechanism

The angular distribution of Compton scattering of two quanta originating in the annihilation of a positron with an electron is investigated as a quantum key distribution (QKD) mechanism in the gamma spectral range. The geometry of coincident Compton scattering is observed on the two sides as a way to obtain partially correlated readings on the quantum channel. We derive the noise probability density function of a conceptually equivalent prepare and measure quantum channel in order to evaluate the limits of the concept in terms of the device secrecy capacity and estimate it at roughly 1.9 bits per 1 000 annihilation events. The high error rate is well above the tolerable error rates of the common reconciliation protocols; therefore, the proposed key agreement protocol by public discussion requires key reconciliation using classical error-correcting codes. We constructed a prototype device based on the readily available monolithic detectors in the least complex setup.

Order Statistics-based “Anti-Bayesian“ Parametric Classification for Asymmetric Distributions in the Exponential Family

Although the field of parametric Pattern Recognition (PR) has been thoroughly studied for over five decades, the use of the Order Statistics (OS) of the distributions to achieve this has not been reported. The pioneering work on using OS for classification was presented in [1] for the Uniform distribution, where it was shown that optimal PR can be achieved in a counter-intuitive manner, diametrically opposed to the Bayesian paradigm, i.e., by comparing the testing sample to a few samples distant from the mean. This must be contrasted with the Bayesian paradigm in which, if we are allowed to compare the testing sample with only a single point in the feature space from each class, the optimal strategy would be to achieve this based on the (Mahalanobis) distance from the corresponding central points, for example, the means. In [2], we showed that the results could be extended for a few symmetric distributions within the exponential family. In this paper, we attempt to extend these results significantly by considering asymmetric distributions within the exponential family, for some of which even the closed form expressions of the cumulative distribution functions are not available. These distributions include the Rayleigh, Gamma and certain Beta distributions. As in [1] and [2], the new scheme, referred to as Classification by Moments of Order Statistics (CMOS), attains an accuracy very close to the optimal Bayes’ bound, as has been shown both theoretically and by rigorous experimental testing.

Laser Excited Nuclear γ -Source of High Spectral Brightness

This paper considers various channels of gammaquantum generation via an ultra-short high-power laser pulse interaction with different targets.We analyse the possibilities to create a pulsed gamma-radiation source using laser triggering of some nuclear reactions and isomer targets. It is shown that sub-MeV monochromatic short pulse of gamma-radiation can be obtained with pulse energy of sub-mJ level from isomer target irradiated by intense laser pulse. For nuclear reaction channel in light- atom materials, it is shown that sub-PW laser pulse gives rise to formation about million gamma-photons of multi-MeV energy.

Effect of Gamma Irradiation on the Microhardness of Polymer Blends of Poly (Ethyl Methacrylate)(Pema) and Poly (Ethylene Oxide) (Peo)

The effect of gamma irradiation on micro-hardness of polymer blends of poly (ethyl methacrylate)(PEMA) and poly (ethylene oxide) (PEO) has been investigated to detect the radiation induced crosslinking. The blend system comprises a noncrystallizable polymer, PEMA and a crystallizable polymer, PEO. On irradiation, the overall hardness of the blend specimens for different dose levels infers occurrence of a crosslinking process. The radiation-induced crosslinking was greater for blends having lower concentration of PEO. However, increase in radiation dose causes softening of blend system due to radiation induced scissioning of the chains

Discrimination of Alcoholic Subjects using Second Order Autoregressive Modelling of Brain Signals Evoked during Visual Stimulus Perception

In this paper, a second order autoregressive (AR) model is proposed to discriminate alcoholics using single trial gamma band Visual Evoked Potential (VEP) signals using 3 different classifiers: Simplified Fuzzy ARTMAP (SFA) neural network (NN), Multilayer-perceptron-backpropagation (MLP-BP) NN and Linear Discriminant (LD). Electroencephalogram (EEG) signals were recorded from alcoholic and control subjects during the presentation of visuals from Snodgrass and Vanderwart picture set. Single trial VEP signals were extracted from EEG signals using Elliptic filtering in the gamma band spectral range. A second order AR model was used as gamma band VEP exhibits pseudo-periodic behaviour and second order AR is optimal to represent this behaviour. This circumvents the requirement of having to use some criteria to choose the correct order. The averaged discrimination errors of 2.6%, 2.8% and 11.9% were given by LD, MLP-BP and SFA classifiers. The high LD discrimination results show the validity of the proposed method to discriminate between alcoholic subjects.

Radiological Risk Assessment in Soil Samples of Western Haryana, India

The measurements of 226Ra, 232Th and 40K using gamma spectrometry and radon concentration and exhalation rates measurements using solid state nuclear track (LR-115, Type-II plastic) detectors are used to asses a first order exposure risk for the persons residing in Fatehbad and Hissar districts of Western Haryana, India. The concentration of Radium, Thorium and Potassium in the soil samples varies from 13.37 Bq m-3 to 24.67 Bq m-3, 34.67 Bq m-3 to 67.34 Bq m-3 and 298.78 Bq m-3 to 405.67 Bq m-3 respectively with average values of 18.78, 47.35 and 361.57 Bq m-3 respectively. The radium equivalent activity (Raeq) calculated for the same soil samples varies from 92.72 Bq m-3 to 140.6 Bq m-3 with an average value of 111.80 Bq m-3. The values of absorbed dose and annual effective dose (indoors and outdoors) are found to vary from 44.18 nGy h-1 to 65.23 nGy h-1, 0.22 mSv y-1 to 0.32 mSv y-1 and 0.05 mSv y-1 to 0.08 mSv y-1 respectively. The radon concentration and exhalation rates have also been reported. The radium equivalent activities in all the soil samples were found to be lower than the limit (370 Bq kg-1) set in the Organization for Economic Cooperation and Development (OECD) report and the value of Hex in all the samples is less than unity.