Callusing in Stevia rebaudiana (Natural Sweetener) for Steviol Glycoside Production

Stevia rebaudiana Bertoni (natural sweetener) belongs to Asteraceae family and can be used as substitute of artificial sweeteners for diabetic patients. Conventionally, it is cultivated by seeds or stem cutting, but seed viability rate is poor. A protocol for callus induction and multiplication was developed to produce large no. of calli in short period. Surface sterilized nodal, leaf and root explants were cultured on Murashige and Skoog (MS) medium with different concentrations of plant hormone like, IBA, kinetin, NAA, 2,4-D, and NAA in combination with 2,4-D. 100% callusing was observed from leaf explants cultured on combination of NAA and 2,4-D after three weeks while with 2,4-D, only 10% callusing was observed. Calli obtained from leaf and root explants were shiny green while with nodal explants it was hard and brown. The present findings deal with induction of callusing in Stevia to achieve the rapid callus multiplication for study of steviol glycosides in callus culture.

Polyphenolic Profile and Antioxidant Activities of Nigella Sativa Seed Extracts In Vitro and In Vivo

Nigella sativa L. is an aromatic plant belonging to the family Ranunculaceae. It has been used traditionally, especially in the middle East and India, for the treatment of asthma, cough, bronchitis, headache, rheumatism, fever, influenza and eczema. Several biological activities have been reported in Nigella sativa seeds, including antioxidant. In this context we tried to estimate the antioxidant activity of various extracts prepared from Nigella sativa seeds, methanolic extract (ME), chloroformic extract (CE), hexanic extract (HE : fixed oil), ethyl acetate extract (EAE) water extract (WE). The Folin-Ciocalteu assay showed that CE and EAE contained high level of phenolic compounds 81.31 and 72.43μg GAE/mg of extract respectively. Similarly, the CE and EAE exhibited the highest DPPH radical scavenging activity, with IC50 values of 106.56μg/ml and 121.62μg/ml respectively. In addition, CE and HE showed the most scavenging activity against superoxide radical generated in the PMS-NADH-NBT system with respective IC50 values of 361.86 μg/ml and 371.80 μg/ml, which is comparable to the activity of the standard antioxidant BHT (344.59 μg/ml). Ferrous ion chelating capacity assay showed that WE, EAE and ME are the most active with 40.57, 39.70 and 22.02 mg EDTA-E/g of extract. The inhibition of linoleic acid/ß-carotene coupled oxidation was estimated by ßcarotene bleaching assay, this showed a highest relative antioxidant activity with CE and EAE (69.82% of inhibition). The antioxidant activities of the methanolic extract and the fixed oil are confirmed by an in vivo assay in mice, the daily oral administration of methanolic extract (500 and 800 mg/kg/day) and fixed oil (2 and 4 ml/kg/day) during 21 days, resulted in a significant enhancement of the blood total antioxidant capacity (measured by KRL test) and the plasmatic antioxidant capacity towards DPPH radical.

The Practical Delivery Room Experience of Nursing Students at Suranaree University of Technology

The purpose of this study was to study the practical delivery room experience of nursing students. The respondents were 6 junior nursing students of Suranaree University of Technology who had a direct experience from practicing in a delivery room between January 9 and March 30, 2012 as part of Nursing Care of the Family and Midwifery 3. The data was collected by using in-depth interview, observation, and reflective report. The results of the study found that the practical delivery room experience of nursing students consisted of three issues: 1) stress and coping with stress during practical exercise, 2) changes in daily routine, and 3) source during practical exercise. The results of this study would lead to the understanding of the meaning of the practical exercise of nursing students.

Cutting and Breaking Events in Telugu

This paper makes a contribution to the on-going debate on conceptualization and lexicalization of cutting and breaking (C&B) verbs by discussing data from Telugu, a language of India belonging to the Dravidian family. Five Telugu native speakers- verbalizations of agentive actions depicted in 43 short video-clips were analyzed. It was noted that verbalization of C&B events in Telugu requires formal units such as simple lexical verbs, explicator compound verbs, and other complex verb forms. The properties of the objects involved, the kind of instruments used, and the manner of action had differential influence on the lexicalization patterns. Further, it was noted that all the complex verb forms encode 'result' and 'cause' sub-events in that order. Due to the polysemy associated with some of the verb forms, our data does not support the straightforward bipartition of this semantic domain.

Effect of Green Water and Mixed Zooplankton on Growth and Survival in Neon Tetra, Paracheirodon innesi (Myers, 1936) during Larval and Early Fry Rearing

Larval rearing and seed production of most of tetra fishes (Family: Characidae) is critical due to their small size larvae and limited numbers of spawning attempts. During the present study the effect of different live foods on growth and survival of neon tetra, Paracheirodon innesi larvae (length 3.1 ± 0.012mm, weight 0.048 ± 0.00015mg) and early fry (length = 6.44 ± 0.025mm, weight = 0.64 ± 0.003mg and 13 days old) was determined in two experiments. Experiment I was conducted for rearing the larvae by using mixed green water and Infusoria whereas, in Experiment II, early fry were fed with mixed zooplankton, decapsulated Artemia cyst and Artemia nauplii. The larvae fed on mixed green water showed significant (p

Effects of Environmental Factors on Polychaete Assemblage in Penang National Park, Malaysia

Macrobenthos distribution along the coastal waters of Penang National Park was studid to estimate the effect of different environmental parameters at three stations, during six sampling months, from June 2010 to April 2011. The aim of this survey was to investigate different environment stress over soft bottom polychaete community along Teluk Ketapang and Pantai Acheh (Penang National Park) over a year period. Variations in the polychaete community were evaluated using univariate and multivariate methods. A total of 604 individuals were examined which was grouped into 23 families. Family Nereidae was the most abundant (22.68%), followed by Spionidae (22.02%), Hesionidae (12.58%), Nephtylidae (9.27%) and Orbiniidae (8.61%). It is noticeable that good results can only be obtained on the basis of good taxonomic resolution. The maximum Shannon-Wiener diversity (H'=2.16) was recorded at distance 200m and 1200m (August 2010) in Teluk Ketapang and lowest value of diversity was found at distance 1200m (December 2010) in Teluk Ketapang.

Scenarios of Societal Security and Business Continuity Cycles

Societal security, continuity scenarios and methodological cycling approach explained in this article. Namely societal security organizational challenges ask implementation of international standards BS 25999-2 & global ISO 22300 which is a family of standards for business continuity management system. Efficient global organization system is distinguished of high entity´s complexity, connectivity & interoperability, having not only cooperative relations in a fact. Competing business have numerous participating ´enemies´, which are in apparent or hidden opponent and antagonistic roles with prosperous organization system, resulting to a crisis scene or even to a battle theatre. Organization business continuity scenarios are necessary for such ´a play´ preparedness, planning, management & overmastering in real environments.

A Family of Affine Projection Adaptive Filtering Algorithms With Selective Regressors

In this paper we present a general formalism for the establishment of the family of selective regressor affine projection algorithms (SR-APA). The SR-APA, the SR regularized APA (SR-RAPA), the SR partial rank algorithm (SR-PRA), the SR binormalized data reusing least mean squares (SR-BNDR-LMS), and the SR normalized LMS with orthogonal correction factors (SR-NLMS-OCF) algorithms are established by this general formalism. We demonstrate the performance of the presented algorithms through simulations in acoustic echo cancellation scenario.

An Improved Construction Method for MIHCs on Cycle Composition Networks

Many well-known interconnection networks, such as kary n-cubes, recursive circulant graphs, generalized recursive circulant graphs, circulant graphs and so on, are shown to belong to the family of cycle composition networks. Recently, various studies about mutually independent hamiltonian cycles, abbreviated as MIHC-s, on interconnection networks are published. In this paper, using an improved construction method, we obtain MIHC-s on cycle composition networks with a much weaker condition than the known result. In fact, we established the existence of MIHC-s in the cycle composition networks and the result is optimal in the sense that the number of MIHC-s we constructed is maximal.

A Practical Approach for Electricity Load Forecasting

This paper is a continuation of our daily energy peak load forecasting approach using our modified network which is part of the recurrent networks family and is called feed forward and feed back multi context artificial neural network (FFFB-MCANN). The inputs to the network were exogenous variables such as the previous and current change in the weather components, the previous and current status of the day and endogenous variables such as the past change in the loads. Endogenous variable such as the current change in the loads were used on the network output. Experiment shows that using endogenous and exogenous variables as inputs to the FFFBMCANN rather than either exogenous or endogenous variables as inputs to the same network produces better results. Experiments show that using the change in variables such as weather components and the change in the past load as inputs to the FFFB-MCANN rather than the absolute values for the weather components and past load as inputs to the same network has a dramatic impact and produce better accuracy.

Forms of Promotion and Dissemination of Traditional Local Wisdom: Creating Occupations among the Elderly in Noanmueng Community, Muang Sub-District, Baan Doong District, Udonthani Province

This research sought to discover the forms of promotion and dissemination of traditional local wisdom that are used to create occupations among the elderly at Noanmueng Community, Muang Sub-District, Baan Doong District, Udornthani Province. The criteria used to select the research sample group were: having a role involved in the promotion and dissemination of traditional local wisdom to create occupations among the elderly; being an experienced person who the residents of Noanmueng Community find trustworthy; and having lived in Noanmueng Community for a long time so as to be able to see the development and change that occurs. A total of 16 persons were thus selected. Data was gathered through a qualitative study, using semi-structured indepth interviews. The collected data was then summarized and discussed according to the research objectives. Finally, the data was presented in narrative format. Results found that the identifying traditional local wisdom of the community (which grew from the residents’ experience and beneficial usage in daily life, passed down from generation to generation) was the weaving of cloth and basketry. As for the manner of promotion and dissemination of traditional local wisdom, these skills were passed down through teaching by example to family members, relatives and others in the community. This was largely the initiative of the elders or elderly members of the community. In order for the promotion and dissemination of traditional local wisdom to create occupations among the elderly, the traditional local wisdom should be supported in every way through participation of the community members. For example, establish a museum of traditional local wisdom for the collection of traditional local wisdom in various fields, both from the past and present innovations. This would be a source of pride for the community, simultaneously helping traditional local wisdom to become widely known and to create income for the community’s elderly. Additional ways include organizing exhibitions of products made by traditional local wisdom, finding both domestic and international markets, as well as building both domestic and international networks aiming to find opportunities to market products made by traditional local wisdom.

Information Entropy of Isospectral Hydrogen Atom

The position and momentum space information entropies of hydrogen atom are exactly evaluated. Using isospectral Hamiltonian approach, a family of isospectral potentials is constructed having same energy eigenvalues as that of the original potential. The information entropy content is obtained in position space as well as in momentum space. It is shown that the information entropy content in each level can be re-arranged as a function of deformation parameter.

Towards Synthesis of Atropodiastereomeric Indolostilbenes Hybrids: A New Class of Oligostilbenoids

The conceptually construction of axially chiral indolostilbenesi.eN-(2-{(E)-2-[2'-(1-Acetyl-1H-indol-2-yl)-3'chloro-4,4',6,6'-tetramethoxy[1,1'-biphenyl]-2yl]ethenyl}phenyl)acetamide and N-(2-{(E)-2-[2'-(1-Acetyl-1H-indol-2-yl)-3'-chloro-2,4',6,6'-tetramethoxy[1,1'-biphenyl]-4-yl]ethenyl}phenyl) acetamide are described in this paper. These structure, were obtained by the tactical combination of palladium-catalyzed coupling which produced 10-acetamido-3,5-dimethoxystilbene, follow by FeCl3-induced oxidative cyclization/dimerisation. All structures were unambiguously confirmed by 1D (1H, 13C) and 2D NMR experiment, (COSY, HMQC, HMBC) and mass spectrometry.