Abstract: Despite the wide spread use of synthetic dyes, natural
dyes are still exploited and used to enhance its inherent aesthetic
qualities as a major material for beautification of the body. Centuries
before the discovery of synthetic dyes, natural dyes were the only
source of dye open to mankind. Dyes are extracted from plant -
leaves, roots and barks, insect secretions, and minerals. However,
research findings have made it clear that of all, plants- leaves, roots,
barks or flowers are the most explored and exploited in which henna
(Lawsonia innermis L.) is one of those plants. Experiment has also
shown that henna is used in body painting in conjunction with an
alkaline (Ammonium Sulphate) as a fixing agent. This of course
gives a clue that if colour derived from henna is properly
investigated, it may not only be used for body decoration but
possibly, may have affinity to fiber substrate. This paper investigates
the dyeing potentials – dye ability and fastness qualities of henna dye
extracts on cotton and linen fibers using mordants like ammonium
sulphate and other alkalis (hydrosulphate and caustic soda, potash,
common salt, potassium alum). Hot and cold water and ethanol
solvent were used in the extraction of the dye to investigate the most
effective method, dye ability, and fastness qualities of these extracts
under room temperature. The results of the experiment show that
cotton have a high rate of dye intake than other fiber. On a similar
note, the colours obtained depend most on the solvent used. In
conclusion, hot water extraction appears more effective. While the
colours obtained from ethanol and both cold hot methods of
extraction range from light to dark yellow, light green to army green
and to some extent shades of brown hues.
Abstract: This study investigates the influence of low
temperature thermo-chemical pretreatment of organic food waste on
performance of COD solubilisation. Both temperature and alkaline
agent were reported to have effect on solubilizing any possible
biomass including organic food waste. The three independent
variables considered in this pretreatment were temperature (50-90oC),
pretreatment time (30-120 minutes) and alkaline concentration,
sodium hydroxide, NaOH (0.7-15 g/L). The maximal condition
obtained were 90oC, 15 g/L NaOH for 2 hours. Solubilisation has
potential in enhancing methane production by providing high amount
of soluble components at early stage during anaerobic digestion.
Abstract: In this article was evaluated the protective effect of
the alternative concrete obtained from the binary mixture of fly ash,
and iron and steel slag. After mixing the cement with aggregates,
structural steel was inserted in the matrix cementitious. The study
was conducted comparatively with specimens exposed to natural
conditions free of chloride ion. The chloride ion effect on the
specimens accelerated under controlled conditions (3.5% NaCl and
25°C temperature). The impedance data were acquired in a range of 1
mHz to 100 kHz.
Abstract: The fundamental issue in understanding the origin and
growth mechanism of nanomaterials, from a fundamental unit is a big
challenging problem to the scientists. Recently, an immense attention
is generated to the researchers for prediction of exceptionally stable
atomic cluster units as the building units for future smart materials.
The present study is a systematic investigation on the stability and
electronic properties of a series of bimetallic (semiconductor-alkaline
earth) clusters, viz., BxMg3 (x=1-5) is performed, in search for
exceptional and/ or unusual stable motifs. A very popular hybrid
exchange-correlation functional, B3LYP along with a higher basis
set, viz., 6-31+G[d,p] is employed for this purpose under the density
functional formalism. The magic stability among the concerned
clusters is explained using the jellium model. It is evident from the
present study that the magic stability of B4Mg3
cluster arises due to
the jellium shell closure.
Abstract: Green concrete are generally composed of recycling
materials as hundred or partial percent substitutes for aggregate,
cement, and admixture in concrete. To reduce greenhouse gas
emissions, efforts are needed to develop environmentally friendly
construction materials. Using of fly ash based geopolymer as an
alternative binder can help reduce CO2 emission of concrete. The
binder of geopolymer concrete is different from the ordinary Portland
cement concrete. Geopolymer Concrete specimens were prepared
with different concentration of NaOH solution M10, M14, and, M16
and cured at 60ºC in duration of 24 hours and 8 hours, in addition to
the curing in direct sunlight. Thus, it is necessary to study the effects
of the geopolymer binder on the behavior of concrete. Concrete is
made by using geopolymer technology is environmental friendly and
could be considered as part of the sustainable development. In this
study, the Local Alkaline Activator in Egypt and crashed stone as
coarse aggregate in fly ash based-geopolymer concrete was
investigated. This paper illustrates the development of mechanical
properties. Since the gained compressive strength for geopolymer
concrete at 28 days was in the range of 22.5MPa – 43.9MPa.
Abstract: Ceramic obtained on the base of aluminum oxide has
wide application range, because it has unique properties, for example,
wear-resistance, dielectric characteristics, and exploitation ability at
high temperatures and in corrosive atmosphere. Low temperature
synthesis of α-Al2O3 is energo-economical process and it is topical
for developing technologies of corundum ceramics fabrication. In the present work possibilities of low temperature transformation
of oxyhydroxides in α-Al2O3, during the presence of small amount of
rare–earth elements compounds (also Th, Re), have been discussed.
Aluminum unstable oxyhydroxides have been obtained by hydrolysis
of aluminium isopropoxide, nitrates, sulphate, and chloride in
alkaline environment at 80-90ºC temperatures. β-Al(OH)3 has been
received from aluminum powder by ultrasonic development. Drying
of oxyhydroxide sol has been conducted with presence of various
types seeds, which amount reaches 0,1-0,2% (mas). Neodymium,
holmium, thorium, lanthanum, cerium, gadolinium, disprosium
nitrates and rhenium carbonyls have been used as seeds and they
have been added to the sol specimens in amount of 0.1-0.2% (mas)
calculated on metals. Annealing of obtained gels is carried out at 70–
1100ºC for 2 hrs. The same specimen transforms in α-Al2O3 at
1100ºC. At this temperature in case of presence of lanthanum and
gadolinium transformation takes place by 70-85%. In case of
presence of thorium stabilization of γ-and θ-phases takes place. It is
established, that thorium causes inhibition of α-phase generation at
1100ºC, and at the time when in all other doped specimens α-phase is
generated at lower temperatures (1000-1050ºC). Synthesis of various
type compounds and simultaneous consolidation has developed in the
furnace of OXY-GON. Composite materials containing oxide and
non-oxide components close to theoretical data have been obtained in
this furnace respectively. During the work the following devices have
been used: X-ray diffractometer DRON-3M (Cu-Kα, Ni filter,
2º/min), High temperature vacuum furnace OXY-GON, electronic
scanning microscopes Nikon ECLIPSE LV 150, NMM-800TRF,
planetary mill Pulverisette 7 premium line, SHIMADZU Dynamic
Ultra Micro Hardness Tester, DUH-211S, Analysette 12 Dyna sizer.
Abstract: Oxygen Reduction Reaction (ORR) performance of
iron and nitrogen co-doped porous carbon nanoparticles (Fe-NPC)
with various physical and (electro) chemical properties have been
investigated. Fe-NPC nanoparticles are synthesized via a facile
soft-templating procedure by using Iron (III) chloride hexa-hydrate as
iron precursor and aminophenol-formaldehyde resin as both carbon
and nitrogen precursor. Fe-NPC nanoparticles shows high surface area
(443.83 m2g-1), high pore volume (0.52 m3g-1), narrow mesopore size
distribution (ca. 3.8 nm), high conductivity (IG/ID=1.04), high kinetic
limiting current (11.71 mAcm-2) and more positive onset potential
(-0.106 V) compared to metal-free NPC nanoparticles (-0.295V)
which make it high efficient ORR metal-free catalysts in alkaline
solution. This study may pave the way of feasibly designing iron and
nitrogen containing carbon materials (Fe-N-C) for highly efficient
oxygen reduction electro-catalysis.
Abstract: Groundwater is vital to the livelihoods and health of the majority of the people, since it provides almost the entire water resource for domestic, agricultural and industrial uses. Groundwater quality comprises the physical, chemical and bacteriological qualities. The present investigation was carried out to determine the physicochemical and bacteriological quality of the ground water sources in the residential areas of Karakulam Grama Panchayath in Thiruvananthapuram district, Kerala state in India. Karakulam is located in the eastern suburbs of Thiruvananthapuram city. The major drinking water source of the residents in the study area is wells. The present study aims to assess the portability and irrigational suitability of groundwater in the study area. The water samples were collected from randomly selected dug wells and bore wells in the study area during post monsoon and pre monsoon seasons of the year 2014 after a preliminary field survey. The physical, chemical and bacteriological parameters of the water samples were analyzed following standard procedures. The concentration of heavy metals (Cd, Pb and Mn) in the acid digested water samples were determined by using an Atomic Absorption Spectrophotometer. The results showed that the pH of well water samples ranged from acidic to alkaline level. In majority of well water samples (>54 %) the iron and magnesium content were found high in both the seasons studied, and the values were above the permissible limits of WHO drinking water quality standards. Bacteriological analyses showed that 63% of the wells were contaminated with total coliforms in both the seasons studied. Irrigational suitability of groundwater was assessed by determining the chemical indices like Sodium Percentage (%Na), Sodium Adsorption Ratio (SAR), Residual Sodium Carbonate (RSC), Permeability Index (PI), and the results indicate that the well water in the study area are good for irrigation purposes. Therefore, the study reveals the degradation of drinking water quality groundwater sources in Karakulam Grama Panchayath in Thiruvananthapuram District, Keralain terms of its chemical and bacteriological characteristics, and is not potable without proper treatment. In the study, more than 1/3rdof the well water samples tested were positive for total coliforms, and the bacterial contamination may pose threat to public health. The study recommends the need for periodic well water quality monitoring in the study area and to conduct awareness programs among the residents.
Abstract: Rice bran is normally used as a raw material for rice
bran oil production or sold as feed with a low price. Conventionally,
the protein in defatted rice bran was extracted using alkaline
extraction and acid precipitation, which involves in chemical usage
and lowering some nutritious component. This study was conducted
in order to extract of rice bran protein concentrate (RBPC) from
defatted rice bran using enzymes and employing polysaccharides in a
precipitating step. The properties of RBPC obtained will be compared
to those of a control sample extracted using a conventional method.
The results showed that extraction of protein from rice bran using
enzymes exhibited the higher protein recovery compared to that
extraction with alkaline. The extraction conditions using alcalase 2%
(v/w) at 50 C, pH 9.5 gave the highest protein (2.44%) and yield
(32.09%) in extracted solution compared to other enzymes. Rice bran
protein concentrate powder prepared by a precipitation step using
alginate (protein in solution: alginate 1:0.016) exhibited the highest
protein (27.55%) and yield (6.84%). Precipitation using alginate was
better than that of acid. RBPC extracted with alkaline (ALK) or
enzyme alcalase (ALC), then precipitated with alginate (AL)
(samples RBP-ALK-AL and RBP-ALC-AL) yielded the precipitation
rate of 75% and 91.30%, respectively. Therefore, protein
precipitation using alginate was then selected. Amino acid profile of
control sample, and sample precipitated with alginate, as compared to
casein and soy protein isolated, showed that control sample showed
the highest content among all sample. Functional property study of
RBP showed that the highest nitrogen solubility occurred in pH 8-10.
There was no statically significant between emulsion capacity and
emulsion stability of control and sample precipitated by alginate.
However, control sample showed a higher of foaming capacity and
foaming stability compared to those of sample precipitated with
alginate. The finding was successful in terms of minimizing
chemicals used in extraction and precipitation steps in preparation of
rice bran protein concentrate. This research involves in a production
of value-added product in which the double amount of protein (28%)
compared to original amount (14%) contained in rice bran could be
beneficial in terms of adding to food products e.g. healthy drink with
high protein and fiber. In addition, the basic knowledge of functional
property of rice bran protein concentrate was obtained, which can be
used to appropriately select the application of this value-added
product from rice bran.
Abstract: Diagnostic enzymes like aspartate aminotransferase
(AST), alanine aminotransferase (ALT) and alkaline phosphatase
(ALP) were determined as indices of heavy metal pollution in Tilapia
guinensis. Three different sets of fishes treated with lead (Pb), iron
(Fe) and copper (Cu) were used for the study while a fourth group
with no heavy metal served as a control. Fishes in each of the groups
were exposed to 2.65mg/l of Pb, 0.85mg/l of Fe and 0.35 mg/l of Cu
in aerated aquaria for 96 hours. Tissue fractionation of the liver
tissues was carried out and the three diagnostic enzymes (AST, ALT,
and ALP) were estimated. Serum levels of the same diagnostic
enzymes were also measured. The mean values of the serum enzyme
activity for ALP in each experimental group were 19.5±1.62,
29.67±2.17 and 1.15±0.27 IU/L for Pb, Fe and Cu groups compared
with 9.99±1.34 IU/L enzyme activity in the control. This result
showed that Pb and Fe caused increased release of the enzyme into
the blood circulation indicating increased tissue damage while Cu
caused a reduction in the serum level as compared with the level in
the control group. The mean values of enzyme activity obtained in
the liver were 102.14±6.12, 140.17±2.06 and 168.23±3.52 IU/L for
Pb, Fe and Cu groups, respectively compared to 91.20±9.42 IU/L
enzyme activity for the control group. The serum and liver AST and
ALT activities obtained in Pb, Fe, Cu and control groups are
reported. It was generally noted that the presence of the heavy metal
caused liver tissues damage and consequent increased level of the
diagnostic enzymes in the serum.
Abstract: Through use of novel modern/rapid processing
techniques such as screen printing and Near-Infrared (NIR) radiative
curing, process time for the sintering of sintered nickel plaques,
applicable to alkaline nickel battery chemistries, has been drastically
reduced from in excess of 200 minutes with conventional convection
methods to below 2 minutes using NIR curing methods. Steps have
also been taken to remove the need for forming gas as a reducing
agent by implementing carbon as an in-situ reducing agent, within the
ink formulation.
Abstract: Mangosteen (Garcinia mangostana) pericarp is
considered as agricultural waste and not fully utilized in food
products. It is widely reported that mangosteen pericarp contains high
antioxidant properties. The objective of this study is to develop novel
yellow alkaline noodle (YAN) substituted with different levels of
mangosteen pericarp powder (MPP). YAN formulation was
substituted with different levels of MPP (0%, 5%, 10% and 15%).
The effect on nutritional and antioxidant properties was evaluated.
Higher substitution levels of MPP resulted in significant increase
(p
Abstract: In this investigation, we have evaluated the effects of
arsenic trioxide on hepatic function in pregnant and lactating Swiss
albino mice and their suckling pups. Experiments were carried out on
female mice given 175 ppm As2O3 in their drinking water from the
14th day of pregnancy until day 14 after delivery. Our results showed
a significant decrease in plasma levels of total protein and albumin,
cholesterol and triglyceride in As2O3 treated mice and their pups. The
hyperbilirubinemia and the increased plasma total alkaline
phosphatase activity suggested the presence of cholestasis.
Transaminase activities as well as lactate deshydrogenase activity in
plasma, known as biomarkers of hepatocellular injury, were elevated
indicating hepatic cells’ damage after treatment with As2O3.
Exposure to arsenic led to an increase of liver thiobarbituric acid
reactive substances level along with a concomitant decrease in the
activities of superoxide dismutase, catalase and glutathione
peroxidase and in glutathione.
Abstract: Although, arsenic trioxide has been the subject of
toxicological research, in vitro cytotoxicity and genotoxicity studies
using relevant cell models and uniform methodology are not well
elucidated. Hence, the aim of the present study was to evaluate the
cytotoxicity and genotoxicity induced by arsenic trioxide in human
keratinocytes (HaCaT) using the MTT [3-(4, 5-dimethylthiazol-2-yl)-
2,5-diphenyltetrazolium bromide] and alkaline single cell gel
electrophoresis (Comet) assays, respectively. Human keratinocytes
were treated with different doses of arsenic trioxide for 4 h prior to
cytogenetic assessment. Data obtained from the MTT assay indicated
that arsenic trioxide significantly reduced the viability of HaCaT cells
in a dose-dependent manner, showing an IC50 value of 34.18 ± 0.6
μM. Data generated from the comet assay also indicated a significant
dose-dependent increase in DNA damage in HaCaT cells associated
with arsenic trioxide exposure. We observed a significant increase in
comet tail length and tail moment, showing an evidence of arsenic
trioxide -induced genotoxic damage in HaCaT cells. This study
confirms that the comet assay is a sensitive and effective method to
detect DNA damage caused by arsenic.
Abstract: Liver disorders are one of the major problems of the
world. Despite its frequent occurrence, high morbidity and high
mortality, its medical management is currently inadequate. This study
was designed to evaluate the hepatoprotective effect of saponin
extract of the root of Garcinia kola on the integrity of the liver of
paracetamol induced wistar albino rats. Twenty five (25) male adult
wistar albino rats were divided into five (5) groups. Group I was the
Control group that received distilled water only, group II was the
negative control that received 2 g/kg of paracetamol on the 13th day,
and group III, IV and V were pre-treated with 100, 200 and
400mg/kg of the saponin extract before inducing the liver damage on
the 13th day with 2 g/kg of paracetamol. Twenty four (24) h after
administration, the rats were sacrificed and blood samples were
collected. The serum Alanine Transaminase (ALT), Aspartate
Transaminase (AST), Alkaline Phosphatase (ALP) activities,
Bilirubin and conjugated bilirubin, glucose and protein
concentrations were evaluated. The liver was fixed immediately in
Formalin and was processed and stained in Haematoxylin and Eosin
(H&E). Administration of saponin extract from the root of Garcinia
kola significantly decreased paracetamol induced elevated enzymes
in the test group. Also histological observations showed that saponin
extract of the root of Garcinia kola exhibited a significant liver
protection against the toxicant as evident by the cells trying to return
to normal. Saponin extract from the root of Garcinia kola indicated a
protection of structural integrity of the hepatocytic cell membrane
and regeneration of the damaged liver.
Abstract: With demand for primary energy continuously
growing, search for renewable and efficient energy sources has been
high on agenda of our society. One of the most promising energy
sources is biogas technology. Residues coming from dairy industry
and milk processing could be used in biogas production; however,
low efficiency and high cost impede wide application of such
technology. One of the main problems is management and conversion
of organic residues through the anaerobic digestion process which is
characterized by acidic environment due to the low whey pH (
Abstract: Pomegranate (Punica granatum L.) is an ancient fruit of great medical interest and rich source of antioxidants. Pesticides as dimethoate play a crucial role in the occurrence many diseases in plants, animal and human. Therefore the ability of Pomegranate (Punica granatum L.) to alleviate hepatotoxicity induced by organophosphate pesticide dimethoate was investigated. Albino male rats were divided randomly into 4 groups and kept at 7 animals per group in an environmentally controlled condition for 6 weeks. The first group was served as a control group (basal diet), the second group fed on basal diet supplemented with 5% freeze dried pomegranate seeds, the third group fed on 20 ppm dimethoate contaminated diet and the last group fed on dimethoate contaminated diet supplemented with 5% freeze dried pomegranate seeds. The results revealed that administration of dimethoate caused high significant increased in liver functions: alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activities as well as lipid peroxide (malonaldhyde, MDA); on the other hand high significant decreased on glutathione (GSH), glutathione peroxidase (GPx), albumin and total protein were observed. However addition of 5% freeze dried pomegranate seeds significantly improved all previously mentioned parameters. These results indicate the dimethoate induced hepatotoxicity and highlight the protective effect of pomegranate seeds as a potential protective agent against dimethoate induced hepatotoxicity. This may be attributed to the powerful antioxidants (polyphenols, total phenols, and total flavonoids) which present in high levels in pomegranate as well as improving the immunity by activation of antioxidant enzymes GSH and GPx.
Abstract: In this study, six bacterial isolates of a slightly
thermophilic organism from the Sg. Klah hot spring, Malaysia were
successfully isolated and designated as M7T55D1, M7T55D2,
M7T55D3, M7T53D1, M7T53D2 and M7T53D3 respectively. The
bacterial isolates were screened for their cellulose hydrolytic ability
on Carboxymethlycellulose agar medium. The isolated bacterial
strains were identified morphologically, biochemically and
molecularly with the aid of 16S rDNA sequencing. All of the bacteria
showed their optimum growth at a slightly alkaline pH of 7.5 with a
temperature of 55°C. All strains were Gram-negative, non-spore
forming type, strictly aerobic, catalase-positive and oxidase-positive
with the ability to produce thermostable cellulase. Based on BLASTn
results, bacterial isolates of M7T55D2 and M7T53D1 gave the
highest homology (97%) with similarity to Tepidimonas ignava while
isolates M7T55D1, M7T55D3, M7T53D2 and M7T53D3 showed
their closest homology (97%-98%) with Tepidimonas thermarum.
These cellulolytic thermophiles might have a commercial potential to
produce valuable thermostable cellulase.
Abstract: Enzyme activity was evaluated in the intestine of
juvenile dourado (Salminus brasiliensis) fed with diets containing 0,
10 or 20% of lyophilized bovine colostrum (LBC) inclusion for either
30 or 60 days. The intestinal enzymes acid and alkaline phosphatase
(ACP and ALP, respectively), non-specific esterase (NSE), lipase
(LIP), dipeptidyl aminopeptidase IV (DAP IV) and leucine
aminopeptidase (LAP) were studied using histochemistry in four
intestinal segments (S1, S2, S3 and posterior intestine). Weak
proteolitic activity was observed in all intestinal segments for DAP
IV and LAP. The activity of NSE and LIP was also weak in all
intestines, except for the moderate activity of NSE in the S2 of 20%
LBC group after 30 days and in the S1 of 0% LBC group after 60
days. The ACP was detected only in the S2 and S3 of the 10% LBC
group after 30 days. Moderate and strong staining was observed in
the first three intestinal segments for ALP and weak activity in the
posterior intestine. The activity of DAP IV, LAP and ALP were also
present in the cytoplasm of the enterocytes. In the present results,
bovine colostrum feeding did not cause alterations in activity of
intestinal enzymes.
Abstract: In this study, a liquid phase microextraction by hollow fiber (HF-LPME) combined with high performance liquid chromatography-UV detector was applied to preconcentrate and determine trace levels of Cyproheptadine in human urine and plasma samples. Cyproheptadine was extracted from 10 mL alkaline aqueous solution (pH: 9.81) into an organic solvent (n-octnol) which was immobilized in the wall pores of a hollow fiber. Then was back-extracted into an acidified aqueous solution (pH: 2.59) located inside the lumen of the hollow fiber. This method is simple, efficient and cost-effective. It is based on pH gradient and differences between two aqueous phases. In order to optimize the HF-LPME some affecting parameters including the pH of donor and acceptor phases, the type of organic solvent, ionic strength, stirring rate, extraction time and temperature were studied and optimized. Under optimal conditions enrichment factor, limit of detection (LOD) and relative standard deviation (RSD(%), n=3) were up to 112, 15 μg.L−1 and 2.7, respectively.