Abstract: The host response in peri-implant tissue may differ from that in periodontal tissue in a healthy individual. The purpose of this study is to investigate the expression of inflammatory cytokines in peri-implant crevicular fluid (PICF) from single implant with different abutment types in comparison to healthy periodontal tissue. 19 participants with healthy implants and teeth were recruited according to inclusion and exclusion criteria. PICF and gingival crevicular fluid (GCF) was collected using sterile paper points. The expression level of inflammatory cytokines including IL-1α, IL-1β, TNF-α, IFN-γ, IL-6, and IL-8 was assessed using enzyme-linked immunosorbent assay (ELISA). Paired t test was used to compare the expression levels of inflammatory cytokines around natural teeth and peri-implant in PICF and GCF of the same individual. The Independent t-test was used to compare the expression levels of inflammatory cytokines in PICF from titanium and UCLA abutment. Expression of IL-6, TNF-α, and IFN-γ in PICF was not statistically different from GCF among titanium and UCLA abutment group. However, the level of IL-1α in the PICF from the implants with UCLA abutment was significantly higher than GCF (P=0.030). In addition, the level of IL-1β in PICF from the implants with titanium abutment was significantly higher than GCF (P=0.032). When different abutment types was compared, IL-8 expression in PICF from implants with UCLA abutment was significantly higher than titanium abutment (P=0.003).
Abstract: Immunomodulators are substances that alter immune
system via dynamic regulation of messenger molecules. It can be
divided into immunostimulant and immunosuppressant. It can help to
increase immunity of people with a low immune system, and also can
help to normalize an overactive immune system. Aim of this study is
to investigate the effects of in vitro exposure to low and high doses of
several immunomodulators which include caffeine, kaloba and
quercetin on antigen-stimulated whole blood culture cytokine
production. Whole blood samples were taken from 5 healthy males
(age: 32 ± 12 years; weight: 75.7 ± 6.1 kg; BMI: 24.3 ± 1.5 kg/m2)
following an overnight fast with no vigorous activity during the
preceding 24 h. The whole blood was then stimulated with 50 μl of
100 x diluted Pediacel vaccine and low or high dose of
immunomodulators in the culture plate. After 20 h incubation (5%
CO2, 37°C), it was analysed using the Evidence Investigator to
determine the production of cytokines including IL-2, IL-4, IL-10,
IFN-γ, and IL-1α. Caffeine and quercetin showed a tendency towards
decrease cytokine production as the doses were increased. On the
other hand, an upward trend was evident with kaloba, where a high
dose of kaloba seemed to increase the cytokine production. In
conclusion, we found that caffeine and quercetin have potential as
immunosuppressant and kaloba as immunostimulant.
Abstract: Ficus deltoidea from the Moraceae family is a popular
medicinal herb in Malaysia. It possesses strong antioxidant and antiinflammatory
properties. In the present study, the anti-inflammatory
effects of F. deltoidea extract on UVB-irradiated HaCaT
Keratinocytes were investigated. HaCaT Keratinocytes were UVBirradiated
(12.5 mJ/cm3) and were treated with 0.05, 0.08 or 0.1% of
F. deltoidea extract. Cell viability following UVB irradiation was
significantly higher in the groups treated with the F. deltoidea extract
at doses of 0.05, 0.08 or 0.1% than in control group with UVB
irradiation only. Tumor necrosis factor-α (TNF-α), interleukin-1α
(IL-1α), interleukin-6 (IL-6) and cyclooxygenase (COX-2) play
primary roles in the inflammation process upon UV irradiation and
are known to be stimulated by UVB irradiation. Treatment with the
F. deltoidea extract dramatically inhibited the UV-induced TNF-α,
IL-1α, IL-6, and COX-2 expression. These results suggest that the F.
deltoidea extract inhibits the production of pro-inflammatory
cytokines and may be an effective protective agent for the treatment
of skin diseases.
Abstract: In-vitro mouse co-culture of E14 embryonic stem cells
(ESCs) and OP9 stromal cells can recapitulate the earliest stages of
haematopoietic development, not accessible in human embryos,
supporting both haemogenic precursors and their primitive
haematopoietic progeny. 1α, 25-Dihydroxy-vitamin D3 (VD3) has
been demonstrated to be a powerful differentiation inducer for a wide
variety of neoplastic cells, and could enhance early differentiation of
ESCs into blood cells in E14/OP9 co-culture. This study aims to
ascertain whether VD3 is key in promoting differentiation and
suppressing proliferation, by separately investigating the effects of
VD3 on the proliferation phase of the E14 cell line and on stromal
OP9 cells.The results showed that VD3 inhibited the proliferation of
the cells in a dose-dependent manner, quantitatively by decreased cell
number, and qualitatively by alkaline-phosphatase staining that
revealed significant differences between VD3-treated and untreated
cells, characterised by decreased enzyme expression (colourless
cells). Propidium-iodide cell-cycle analyses showed no significant
percentage change in VD3-treated E14 and OP9 cells within their G
and S-phases, compared to the untreated controls, despite the
increased percentage of G-phase compared to the S-phase in a dosedependent
manner. These results with E14 and OP9 cells indicate that
adequate VD3 concentration enhances cellular differentiation and
inhibits proliferation. The results also suggest that if E14 and OP9
cells were co-cultured andVD3-treated, there would be furtherenhanced
differentiation of ESCs into blood cells.