Rice cDNA Encoding PROLM is Capable of Rescuing Salt Sensitive Yeast Phenotypes G19 and Axt3K from Salt Stress

Rice seed expression (cDNA) library in the Lambda Zap 11® phage constructed from the developing grain 10-20 days after flowering was transformed into yeast for functional complementation assays in three salt sensitive yeast mutants S. cerevisiae strain CY162, G19 and Axt3K. Transformed cells of G19 and Axt3K with pYES vector with cDNA inserts showed enhance tolerance than those with empty pYes vector. Sequencing of the cDNA inserts revealed that they encode for the putative proteins with the sequence homologous to rice putative protein PROLM24 (Os06g31070), a prolamin precursor. Expression of this cDNA did not affect yeast growth in absence of salt. Axt3k and G19 strains expressing the PROLM24 were able to grow upto 400 mM and 600 mM of NaCl respectively. Similarly, Axt3k mutant with PROLM24 expression showed comparatively higher growth rate in the medium with excess LiCl (50 mM). The observation that expression of PROLM24 rescued the salt sensitive phenotypes of G19 and Axt3k indicates the existence of a regulatory system that ameliorates the effect of salt stress in the transformed yeast mutants. However, the exact function of the cDNA sequence, which shows partial sequence homology to yeast UTR1 is not clear. Although UTR1 involved in ferrous uptake and iron homeostasis in yeast cells, there is no evidence to prove its role in Na+ homeostasis in yeast cells. Absence of transmembrane regions in Os06g31070 protein indicates that salt tolerance is achieved not through the direct functional complementation of the mutant genes but through an alternative mechanism.

A National Idea in Conditions of the Islamic Revival

Discussion and development of principles of the uniform nation formation within the limits of the Kazakhstan state obviously became one of the most pressing questions of the day. The fact that this question has not been solved "from above" as many other questions has caused really brisk discussion, shows us increase of civil consciousness in Kazakhstan society, and also the actuality of this theme which can be carried in the category of fatal questions. In any sense, nation building has raised civil society to a much higher level. It would be better to begin with certain definitions. First is the word "nation". The second is the "state". Both of these terms are very closely connected with each other, so that in English language they are in general synonyms. In Russian more shades of these terms exist. For example in Kazakhstan the citizens of the country irrespective of nationality (but mainly with reference to non-kazakhs) are called «kazakhstanians», while the name of the title nation is \"Kazakhs\". The same we can see in Russia, where, for example, the Chechen or the Yakut –are \"Rossiyane\" which means “the citizens of Russian Federation, but not \"Russians\". The paper was written under the research project “Islam in modern Kazakhstan: the nature and outcome of the religious revival”.

Implementation of Technology Concept for the Reduction of Cyanobacteria in Laboratory

Following the research in the Department of environmental engineering in Faculty of mechanical engineering on Technical University of Kosice and experiences with electrocoagulation style of disposal waste water, there were designed and partly examining the equipment of two stage revitalization on the standing and little fusible water of tenet electrolysis on the little tarns. With the cooperation with vet experts was that manners prove and it is innocuous for animals, during which time cyanobacteria are totally paralyzed. For the implementation of science and research results have been obtained by means EU funds for structural development.

2-Dimensional Finger Gesture Based Mobile Robot Control Using Touch Screen

The purpose of this study was to present a reliable mean for human-computer interfacing based on finger gestures made in two dimensions, which could be interpreted and adequately used in controlling a remote robot's movement. The gestures were captured and interpreted using an algorithm based on trigonometric functions, in calculating the angular displacement from one point of touch to another as the user-s finger moved within a time interval; thereby allowing for pattern spotting of the captured gesture. In this paper the design and implementation of such a gesture based user interface was presented, utilizing the aforementioned algorithm. These techniques were then used to control a remote mobile robot's movement. A resistive touch screen was selected as the gesture sensor, then utilizing a programmed microcontroller to interpret them respectively.

The Tag Authentication Scheme using Self-Shrinking Generator on RFID System

Since communications between tag and reader in RFID system are by radio, anyone can access the tag and obtain its any information. And a tag always replies with the same ID so that it is hard to distinguish between a real and a fake tag. Thus, there are many security problems in today-s RFID System. Firstly, unauthorized reader can easily read the ID information of any Tag. Secondly, Adversary can easily cheat the legitimate reader using the collected Tag ID information, such as the any legitimate Tag. These security problems can be typically solved by encryption of messages transmitted between Tag and Reader and by authentication for Tag. In this paper, to solve these security problems on RFID system, we propose the Tag Authentication Scheme based on self shrinking generator (SSG). SSG Algorithm using in our scheme is proposed by W.Meier and O.Staffelbach in EUROCRYPT-94. This Algorithm is organized that only one LFSR and selection logic in order to generate random stream. Thus it is optimized to implement the hardware logic on devices with extremely limited resource, and the output generating from SSG at each time do role as random stream so that it is allow our to design the light-weight authentication scheme with security against some network attacks. Therefore, we propose the novel tag authentication scheme which use SSG to encrypt the Tag-ID transmitted from tag to reader and achieve authentication of tag.

Development and Characterization of Normoxic Polyhydroxyethylacrylate (PHEA) Gel Dosimeter using Raman Spectroscopy

Raman spectroscopy are used to characterize the chemical changes in normoxic polyhydroxyethylacrylate gel dosimeter (PHEA) induced by radiation. Irradiations in the low dose region are performed and the polymerizations of PHEA gels are monitored by the observing the changes of Raman shift intensity of the carbon covalent bond of PHEA originated from both monomer and the cross-linker. The variation in peak intensities with absorbed dose was observed. As the dose increase, the peak intensities of covalent bond of carbon in the polymer gels decrease. This point out that the amount of absorbed dose affect the polymerization of polymer gels. As the absorbed dose increase, the polymerizations also increase. Results verify that PHEA gel dosimeters are sensitive even in lower dose region.

Local Curvelet Based Classification Using Linear Discriminant Analysis for Face Recognition

In this paper, an efficient local appearance feature extraction method based the multi-resolution Curvelet transform is proposed in order to further enhance the performance of the well known Linear Discriminant Analysis(LDA) method when applied to face recognition. Each face is described by a subset of band filtered images containing block-based Curvelet coefficients. These coefficients characterize the face texture and a set of simple statistical measures allows us to form compact and meaningful feature vectors. The proposed method is compared with some related feature extraction methods such as Principal component analysis (PCA), as well as Linear Discriminant Analysis LDA, and independent component Analysis (ICA). Two different muti-resolution transforms, Wavelet (DWT) and Contourlet, were also compared against the Block Based Curvelet-LDA algorithm. Experimental results on ORL, YALE and FERET face databases convince us that the proposed method provides a better representation of the class information and obtains much higher recognition accuracies.

Analysis of Plasmids and Restriction Fragment Length Polymorphisms of Acinetobacter baumannii Isolated from Hospitals- AL Jouf Region- KSA

Abstract–The objectives of the current study are to determine the prevalence, etiological agents, drug susceptibility pattern and plasmid profile of Acinetobacter baumannii isolates from Hospital-Acquired Infections (HAI) at Community Hospital, Al Jouf Province, Saudi Arabia. A total of 1890 patients had developed infection during hospital admission and were included in the study. Among those who developed nosocomial infections, 15(9.4), 10(2.7) and 118 (12.7) had respiratory tract infection (RTI), blood stream infections (BSI) and urinary tract (UTI) respectively. A total of 268 bacterial isolates were isolated from nosocomial infection. S. aureus was reported in 23.5% for of the total isolates followed by Klebsiella pneumoniae (17.5%), E. coli (17.2%), P. aeruginosa (11.9%), coagulase negative staphylococcus (9%), A. baumannii (7.1%), Enterobacter spp. (3.4%), Citrobacter freundii (3%), Proteus mirabilis (2.6%), and Proteus vulgaris and Enterococcous faecalis (0.7%). Isolated organisms are multi-drug resistant, predominantly Gram-positive pathogens with a high incidence of methicillin-resistant S. aureus, extended spectrum beta lactamase and vancomycin resistant enterococci organisms. The RFLP (Fragment Length Polymorphisms) patterns of plasmid preparations from isolated A. baumannii isolates had altered RFLP patterns, possibly due to the presence of plasmid(s). Five A. baumannii isolates harbored plasmids all of which were not less than 2.71kbp in molecular weight. Hence, it showed that the gene coding for the isolates were located on the plasmid DNA while the remaining isolates which have no plasmid might showed gene coding for antibiotic resistance being located on chromosomal DNA. Nosocomial infections represent a current problem in Community Hospital, Al Jouf Province, Saudi Arabia. Problems associated with SSI include infection with multidrug resistant pathogens which are difficult to treat and are associated with increased mortality.

Real-time Laser Monitoring based on Pipe Detective Operation

The pipe inspection operation is the difficult detective performance. Almost applications are mainly relies on a manual recognition of defective areas that have carried out detection by an engineer. Therefore, an automation process task becomes a necessary in order to avoid the cost incurred in such a manual process. An automated monitoring method to obtain a complete picture of the sewer condition is proposed in this work. The focus of the research is the automated identification and classification of discontinuities in the internal surface of the pipe. The methodology consists of several processing stages including image segmentation into the potential defect regions and geometrical characteristic features. Automatic recognition and classification of pipe defects are carried out by means of using an artificial neural network technique (ANN) based on Radial Basic Function (RBF). Experiments in a realistic environment have been conducted and results are presented.

Multivariate School Travel Demand Regression Based on Trip Attraction

Since primary school trips usually start from home, attention by many scholars have been focused on the home end for data gathering. Thereafter category analysis has often been relied upon when predicting school travel demands. In this paper, school end was relied on for data gathering and multivariate regression for future travel demand prediction. 9859 pupils were surveyed by way of questionnaires at 21 primary schools. The town was divided into 5 zones. The study was carried out in Skudai Town, Malaysia. Based on the hypothesis that the number of primary school trip ends are expected to be the same because school trips are fixed, the choice of trip end would have inconsequential effect on the outcome. The study compared empirical data for home and school trip end productions and attractions. Variance from both data results was insignificant, although some claims from home based family survey were found to be grossly exaggerated. Data from the school trip ends was relied on for travel demand prediction because of its completeness. Accessibility, trip attraction and trip production were then related to school trip rates under daylight and dry weather conditions. The paper concluded that, accessibility is an important parameter when predicting demand for future school trip rates.

Effects of Wastewater Strength and Salt Stress on Microalgal Biomass Production and Lipid Accumulation

This work aims to investigate a potential of microalgae for utilizing industrial wastewater as a cheap nutrient for their growth and oil accumulation. Wastewater was collected from the effluent ponds of agro-industrial factories (cassava and ethanol production plants). Only 2 microalgal strains were isolated and identified as Scenedesmus quadricauda and Chlorella sp.. However, only S. quadricauda was selected to cultivate in various wastewater concentrations (10%, 20%, 40%, 60%, 80% and 100%). The highest biomass obtained at 6.6×106 and 6.27×106 cells/ml when 60% wastewater was used in flask and photo-bioreactor. The cultures gave the highest lipid content at 18.58 % and 42.86% in cases of S. quadricauda and S. obliquus. In addition, under salt stress (1.0 M NaCl), S. obliquus demonstrated the highest lipid content at 50% which was much more than the case of no NaCl adding. However, the concentration of NaCl does not affect on lipid accumulation in case of S. quadricauda.

Statistics over Lyapunov Exponents for Feature Extraction: Electroencephalographic Changes Detection Case

A new approach based on the consideration that electroencephalogram (EEG) signals are chaotic signals was presented for automated diagnosis of electroencephalographic changes. This consideration was tested successfully using the nonlinear dynamics tools, like the computation of Lyapunov exponents. This paper presented the usage of statistics over the set of the Lyapunov exponents in order to reduce the dimensionality of the extracted feature vectors. Since classification is more accurate when the pattern is simplified through representation by important features, feature extraction and selection play an important role in classifying systems such as neural networks. Multilayer perceptron neural network (MLPNN) architectures were formulated and used as basis for detection of electroencephalographic changes. Three types of EEG signals (EEG signals recorded from healthy volunteers with eyes open, epilepsy patients in the epileptogenic zone during a seizure-free interval, and epilepsy patients during epileptic seizures) were classified. The selected Lyapunov exponents of the EEG signals were used as inputs of the MLPNN trained with Levenberg- Marquardt algorithm. The classification results confirmed that the proposed MLPNN has potential in detecting the electroencephalographic changes.

Thermal Analysis of the Current Path from Circuit Breakers Using Finite Element Method

This paper describes a three-dimensional thermal model of the current path included in the low voltage power circuit breakers. The model can be used to analyse the thermal behaviour of the current path during both steady-state and transient conditions. The current path lengthwise temperature distribution and timecurrent characteristic of the terminal connections of the power circuit breaker have been obtained. The influence of the electric current and voltage drop on main electric contact of the circuit breaker has been investigated. To validate the three-dimensional thermal model, some experimental tests have been done. There is a good correlation between experimental and simulation results.

Exponential Stability Analysis for Switched Cellular Neural Networks with Time-varying Delays and Impulsive Effects

In this Letter, a class of impulsive switched cellular neural networks with time-varying delays is investigated. At the same time, parametric uncertainties assumed to be norm bounded are considered. By dividing the network state variables into subgroups according to the characters of the neural networks, some sufficient conditions guaranteeing exponential stability for all admissible parametric uncertainties are derived via constructing appropriate Lyapunov functional. One numerical example is provided to illustrate the validity of the main results obtained in this paper.

Cardiac Function and Morphological Adaptations in Endurance and Resistance Athletes: Evaluation using a new Method

Background: Tissue Doppler Echocardiography (TDE) assesses diastolic function more accurately than routine pulse Doppler echo. Assessment of the effects of dynamic and static exercises on the heart by using TDE can provides new information about the athlete-s heart syndrome. Methods: This study was conducted on 20 elite wrestlers, 14 endurance runners at national level and 21 non-athletes as the control group. Participants underwent two-dimensional echocardiography, standard Doppler and TDE. Results: Wrestlers had the highest left ventricular mass index, enddiastolic inter-ventricular septum thickness and left ventricular Posterior wall thickness. Runners had the highest Left ventricular end-diastolic volume, LV ejection fraction, stroke volume and cardiac output. In TDE, the early diastolic velocity of mitral annulus to the late diastolic velocity ratio in athletic groups was greater than the controls with no significant difference. Conclusion: In spite of cardiac morphological changes in athletes, TDE shows that cardiac diastolic function won-t be adversely affected.

Using Finite Element Method for Determination of Poles Number in Optimal Design of Linear Motor

One of Effective parameters on the performance of linear induction motors is number of poles which must be selected and optimized to increase power efficiency and motor performance significantly. In this paper a double-sided linear induction motor with different poles number by using MAXWELL3D software is designed and with finite element method is analyzed electromagnetically. Then for dynamic simulation, linear motor by using MATLAB software is simulated. The results show that by adding poles number, system time response is increased and motor after more time reaches to steady state. Also propulsion force of motor is increased.

The Empirical Survey on the Effect of Using Media in Explosive Forming of Tubular Shells

The special and unique advantages of explosive forming, has developed its use in different industries. Considering the important influence of improving the current explosive forming techniques on increasing the efficiency and control over the explosive forming procedure, the effects of air and water as the energy-conveying medium, and also their differences will be illustrated in this paper. Hence, a large number of explosive forming tests have been conducted on two sizes of thin walled cylindrical shells by using air and water as the working medium. Comparative diagrams of the maximum radial deflection of work-pieces of the same size, as a function of the scaled distance, show that for the points with the same values of scaled distance, the maximum radial deformation caused by the under water explosive loading is 4 to 5 times more than the deflection of the shells under explosive forming, while using air. Results of this experimental research have also been compared with other studies which show that using water as the energy conveying media increases the efficiency up to 4.8 times. The effect of the media on failure modes of the shells, and the necking mechanism of the walls of the specimens, while being explosively loaded, are also discussed in this issue. Measuring the tested specimens shows that, the increase in the internal volume has been accompanied by necking of the walls, which finally results in the radial rupture of the structure.

Environmental Management in Arid Regions:The Question of Water

Only recently have water ethics received focused interest in the international water community. Because water is metabolically basic to life, an ethical dimension persists in every decision related to water. Water ethics at once express human society-s approach to water and act as guidelines for behaviour. Ideas around water are often implicit and embedded as assumptions. They can be entrenched in behaviour and difficult to contest because they are difficult to “see". By explicitly revealing the ethical ideas underlying water-related decisions, human society-s relationship with water, and with natural systems of which water is part, can be contested and shifted or be accepted with conscious intention by human society. In recent decades, improved understanding of water-s importance for ecosystem functioning and ecological services for human survival is moving us beyond this growth-driven, supplyfocused management paradigm. Environmental ethics challenge this paradigm by extending the ethical sphere to the environment and thus water or water Resources management per se. An ethical approach is a legitimate, important, and often ignored approach to effect change in environmental decision making. This qualitative research explores principles of water ethics and examines the underlying ethical precepts of selected water policy examples. The constructed water ethic principles act as a set of criteria against which a policy comparison can be established. This study shows that water Resources management is a progressive issue by embracing full public participation and a new planning model, and knowledgegeneration initiatives.

Automatic Fingerprint Classification Using Graph Theory

Using efficient classification methods is necessary for automatic fingerprint recognition system. This paper introduces a new structural approach to fingerprint classification by using the directional image of fingerprints to increase the number of subclasses. In this method, the directional image of fingerprints is segmented into regions consisting of pixels with the same direction. Afterwards the relational graph to the segmented image is constructed and according to it, the super graph including prominent information of this graph is formed. Ultimately we apply a matching technique to compare obtained graph with the model graphs in order to classify fingerprints by using cost function. Increasing the number of subclasses with acceptable accuracy in classification and faster processing in fingerprints recognition, makes this system superior.