Abstract: The cDNA encoding the 326 amino acids of a Class I
basic chitinase gene from Leucaena leucocephala de Wit (KB3,
Genbank accession: AAM49597) was cloned under the control of
CaMV35S promoter in pCAMBIA 1300 and transferred to
Koshihikari. Calli of Koshihikari rice was transformed with
agrobacterium with this construct expressing the chitinase and β-
glucouronidase (GUS). The frequencies of calli 90 % has been
obtained from rice seedlings cultured on NB medium. The high
regeneration frequencies, 74% was obtained from calli cultured on
regeneration medium containing 4 mg/l BAP, and 7 g/l phytagel at
25°C. Various factors were studied in order to establish a procedure
for the transformation of Koshihikari Agrobacterium tumefaciens.
Supplementation of 50 mM acetosyringone to the medium during
coculivation was important to enhance the frequency to transient
transformation. The 4 week-old scutellum-derived calli were
excellent starting materials. Selection medium based on NB medium
supplement with 40 mg/l hygromycin and 400 mg/l cefotaxime were
an optimized medium for selection of transformed rice calli. The
percentage of transformation 70 was obtained. Recombinant calli and
regenerated rice plants were checked the expression of chitinase and
gus by PCR, northern blot gel, southern blot gel, and gus assay.
Chitinase and gus were expressed in all parts of recombinant rice.
The rice line expressing the KB3 chiitnase was more resistant to the
blast fungus Fusarium monoliforme than control line.
Abstract: The UK Government has emphasized the role of Local Authorities as a key player in its flagship residential energy efficiency strategies, by identifying and targeting areas for energy efficiency improvements. Residential energy consumption in England is characterized by significant geographical variation in energy demand, which makes centralized targeting of areas for energy efficiency intervention difficult. This paper draws on research which aims to understand how demographic, social, economic, urban form and climatic factors influence the geographical variations in English residential gas consumption. The paper reports the findings of a multiple regression model that shows how 64% of the geographical variation in residential gas consumption is accounted for by variations in these factors. Results from this study, after further refinement and validation, can be used by Local Authorities to identify areas within their boundaries that have higher than expected gas consumption, these may be prime targets for energy efficiency initiatives.
Abstract: The avian phytohaemagglutinin skin test is being
proved as an in vivo system for the evaluation an avian in vivo T cell
mitogenicity. The test system was one week old Gallus domesticus
broiler Chickens. Five replicates were done for each of the whole,
1:10 dilutions of each of 0.05 IU tuberculin, tetanus immunoglobulin
and DPT vaccine as test materials. The evaluation parameters were
the skin indurations and lymphoblast percentages in bone marrow
lymphocytes.
Tuberculin indurations were 2.06 and 1.26mm for 0.05 IU
respectively while lymphoblast percent were 0.234 and 0.1
accordingly.
The skin indurations of 135mg/ml and 1.35mg/ml tetanus
immunoglobulin were 4.86 and 3.96mm while lymphoblast
percentages were 0.3 and 0.14 respectively.
The whole DPT and 1:10 concentration were with 4.5 and 3.2mm
while their lymphoblast percentages were 0.28 and 0.12 accordingly.
Thus the mitogenicity of the test materials was of dependant type.
Abstract: The soil moisture content is an important property of
the soil. The results of mean weekly gravimetric soil moisture
content, measured for the three soil layers within the A horizon,
showed that it was higher for the top 5 cm over the whole period of
monitoring (15/7/2004 up to 10/11/05) with the variation becoming
greater during winter time. This reflects the pattern of rainfall in
Ireland which is spread over the whole year and shows that light
rainfall events during summer time were compensated by loss
through evapotranspiration, but only in the top 5 cm of soil. This
layer had the highest porosity and highest moisture holding capacity
due to the high content of organic matter. The gravimetric soil
moisture contents of the top 5 cm and the underlying 5-15 and 15-25
cm layers show that bottom site of the Hill Field had higher soil
moisture content than the middle and top sites during the whole
period of monitoring.
Abstract: This is an application research presenting the
improvement of production quality using the six sigma solutions and
the analyses of benefit-cost ratio. The case of interest is the
production of tile-concrete. Such production has faced with the
problem of high nonconforming products from an inappropriate
surface coating and had low process capability based on the strength
property of tile. Surface coating and tile strength are the most critical
to quality of this product. The improvements followed five stages of
six sigma solutions. After the improvement, the production yield was
improved to 80% as target required and the defective products from
coating process was remarkably reduced from 29.40% to 4.09%. The
process capability based on the strength quality was increased from
0.87 to 1.08 as customer oriented. The improvement was able to save
the materials loss for 3.24 millions baht or 0.11 million dollars. The
benefits from the improvement were analyzed from (1) the reduction
of the numbers of non conforming tile using its factory price for
surface coating improvement and (2) the materials saved from the
increment of process capability. The benefit-cost ratio of overall
improvement was high as 7.03. It was non valuable investment in
define, measure, analyses and the initial of improve stages after that
it kept increasing. This was due to there were no benefits in define,
measure, and analyze stages of six sigma since these three stages
mainly determine the cause of problem and its effects rather than
improve the process. The benefit-cost ratio starts existing in the
improve stage and go on. Within each stage, the individual benefitcost
ratio was much higher than the accumulative one as there was an
accumulation of cost since the first stage of six sigma. The
consideration of the benefit-cost ratio during the improvement
project helps make decisions for cost saving of similar activities
during the improvement and for new project. In conclusion, the
determination of benefit-cost ratio behavior through out six sigma
implementation period provides the useful data for managing quality
improvement for the optimal effectiveness. This is the additional
outcome from the regular proceeding of six sigma.
Abstract: In this paper, transversal vibration of buried pipelines
during loading induced by underground explosions is analyzed. The
pipeline is modeled as an infinite beam on an elastic foundation, so
that soil-structure interaction is considered by means of transverse
linear springs along the pipeline. The pipeline behavior is assumed to
be ideal elasto-plastic which an ultimate strain value limits the plastic
behavior. The blast loading is considered as a point load, considering
the affected length at some point of the pipeline, in which the
magnitude decreases exponentially with time. A closed-form solution
for the quasi-static problem is carried out for both elastic and elasticperfect
plastic behaviors of pipe materials. At the end, a comparative
study on steel and polyethylene pipes with different sizes buried in
various soil conditions, affected by a predefined underground
explosion is conducted, in which effect of each parameter is
discussed.
Abstract: This study performs a comparative analysis of the 21 Greek Universities in terms of their public funding, awarded for covering their operating expenditure. First it introduces a DEA/MCDM model that allocates the fund into four expenditure factors in the most favorable way for each university. Then, it presents a common, consensual assessment model to reallocate the amounts, remaining in the same level of total public budget. From the analysis it derives that a number of universities cannot justify the public funding in terms of their size and operational workload. For them, the sufficient reduction of their public funding amount is estimated as a future target. Due to the lack of precise data for a number of expenditure criteria, the analysis is based on a mixed crisp-ordinal data set.
Abstract: The objective of this paper is to estimate realistic
principal extrusion process parameters by means of artificial neural
network. Conventionally, finite element analysis is used to derive
process parameters. However, the finite element analysis of the
extrusion model does not consider the manufacturing process
constraints in its modeling. Therefore, the process parameters
obtained through such an analysis remains highly theoretical.
Alternatively, process development in industrial extrusion is to a
great extent based on trial and error and often involves full-size
experiments, which are both expensive and time-consuming. The
artificial neural network-based estimation of the extrusion process
parameters prior to plant execution helps to make the actual extrusion
operation more efficient because more realistic parameters may be
obtained. And so, it bridges the gap between simulation and real
manufacturing execution system. In this work, a suitable neural
network is designed which is trained using an appropriate learning
algorithm. The network so trained is used to predict the
manufacturing process parameters.
Abstract: The authors present an algorithm for order reduction of linear dynamic systems using the combined advantages of stability equation method and the error minimization by Genetic algorithm. The denominator of the reduced order model is obtained by the stability equation method and the numerator terms of the lower order transfer function are determined by minimizing the integral square error between the transient responses of original and reduced order models using Genetic algorithm. The reduction procedure is simple and computer oriented. It is shown that the algorithm has several advantages, e.g. the reduced order models retain the steady-state value and stability of the original system. The proposed algorithm has also been extended for the order reduction of linear multivariable systems. Two numerical examples are solved to illustrate the superiority of the algorithm over some existing ones including one example of multivariable system.
Abstract: MinC plays an important role in bacterial cell division
system by inhibiting FtsZ assembly. However, the molecular
mechanism of the action is poorly understood. E. coli MinC Nterminus
domain was purified and crystallized using 1.4 M sodium
citrate pH 6.5 as a precipitant. X-ray diffraction data was collected
and processed to 2.3 Å from a native crystal. The crystal belonged to
space group P212121, with the unit cell parameters a = 52.7, b = 54.0,
c = 64.7 Å. Assuming the presence of two molecules in the
asymmetric unit, the Matthews coefficient value is 1.94 Å3 Da-1,
which corresponds to a solvent content of 36.5%. The overall
structure of MinCN is observed as a dimer form through anti-parallel
ß-strand interaction.
Abstract: Analytical solution of the first-order and third-order
shear deformation theories are developed to study the free vibration
behavior of simply supported functionally graded plates. The
material properties of plate are assumed to be graded in the thickness
direction as a power law distribution of volume fraction of the
constituents. The governing equations of functionally graded plates
are established by applying the Hamilton's principle and are solved
by using the Navier solution method. The influence of side-tothickness
ratio and constituent of volume fraction on the natural
frequencies are studied. The results are validated with the known
data in the literature.
Abstract: Supplier selection is a multi criteria decision-making process that comprises tangible and intangible factors. The majority of previous supplier selection techniques do not consider strategic perspective. Besides, uncertainty is one of the most important obstacles in supplier selection. For the first, time in this paper, the idea of the algorithm " Knapsack " is used to select suppliers Moreover, an attempt has to be made to take the advantage of a simple numerical method for solving model .This is an innovation to resolve any ambiguity in choosing suppliers. This model has been tried in the suppliers selected in a competitive environment and according to all desired standards of quality and quantity to show the efficiency of the model, an industry sample has been uses.
Abstract: In this paper back-propagation artificial neural network
(BPANN) is employed to predict the deformation of the upsetting
process. To prepare a training set for BPANN, some finite element
simulations were carried out. The input data for the artificial neural
network are a set of parameters generated randomly (aspect ratio d/h,
material properties, temperature and coefficient of friction). The
output data are the coefficient of polynomial that fitted on barreling
curves. Neural network was trained using barreling curves generated
by finite element simulations of the upsetting and the corresponding
material parameters. This technique was tested for three different
specimens and can be successfully employed to predict the
deformation of the upsetting process
Abstract: In this paper, we proposed a new routing protocol for
Unmanned Aerial Vehicles (UAVs) that equipped with directional
antenna. We named this protocol Directional Optimized Link State
Routing Protocol (DOLSR). This protocol is based on the well
known protocol that is called Optimized Link State Routing Protocol
(OLSR). We focused in our protocol on the multipoint relay (MPR)
concept which is the most important feature of this protocol. We
developed a heuristic that allows DOLSR protocol to minimize
the number of the multipoint relays. With this new protocol the
number of overhead packets will be reduced and the End-to-End
delay of the network will also be minimized. We showed through
simulation that our protocol outperformed Optimized Link State
Routing Protocol, Dynamic Source Routing (DSR) protocol and Ad-
Hoc On demand Distance Vector (AODV) routing protocol in
reducing the End-to-End delay and enhancing the overall
throughput. Our evaluation of the previous protocols was based
on the OPNET network simulation tool.
Abstract: In this paper we designed and implemented a new
ensemble of classifiers based on a sequence of classifiers which were
specialized in regions of the training dataset where errors of its
trained homologous are concentrated. In order to separate this
regions, and to determine the aptitude of each classifier to properly
respond to a new case, it was used another set of classifiers built
hierarchically. We explored a selection based variant to combine the
base classifiers. We validated this model with different base
classifiers using 37 training datasets. It was carried out a statistical
comparison of these models with the well known Bagging and
Boosting, obtaining significantly superior results with the
hierarchical ensemble using Multilayer Perceptron as base classifier.
Therefore, we demonstrated the efficacy of the proposed ensemble,
as well as its applicability to general problems.
Abstract: The inherent iterative nature of product design and development poses significant challenge to reduce the product design and development time (PD). In order to shorten the time to market, organizations have adopted concurrent development where multiple specialized tasks and design activities are carried out in parallel. Iterative nature of work coupled with the overlap of activities can result in unpredictable time to completion and significant rework. Many of the products have missed the time to market window due to unanticipated or rather unplanned iteration and rework. The iterative and often overlapped processes introduce greater amounts of ambiguity in design and development, where the traditional methods and tools of project management provide less value. In this context, identifying critical metrics to understand the iteration probability is an open research area where significant contribution can be made given that iteration has been the key driver of cost and schedule risk in PD projects. Two important questions that the proposed study attempts to address are: Can we predict and identify the number of iterations in a product development flow? Can we provide managerial insights for a better control over iteration? The proposal introduces the concept of decision points and using this concept intends to develop metrics that can provide managerial insights into iteration predictability. By characterizing the product development flow as a network of decision points, the proposed research intends to delve further into iteration probability and attempts to provide more clarity.
Abstract: Green Forestation Plan (GFP) was expected to promote the reforestation of plains totaling 60,000 has within the first 8 years. Annual subsidies were budgeted at $120,000 per ha, and $2.4 million for 20 years. In this research we have surveyed landlords- opinions toward the GFP in an attempt to understand landlords- incentives for participating in the GFP and their levels of concern and agreement toward the policy design. Based our analysis of landlords- opinions on the policy design, we expect to derive appropriate complementary measures, establish effective promotional schemes, and raise the policy effectiveness of the GFP. According to the results of this research, there was still a relatively high proportion of population who were not aware of GFP; more than 50% of landlords were neutral or willing to participate given high reforestation subsidies; approximately 30% of landlords were unwilling to participate. In terms of the designs of GFP, more than 50% of respondents were concerned and agreed with the policy design. In terms of the period of this policy, 52.7% of respondents indicated that it should be shortened to 15 years or lower. In terms of the amount of the subsidy, 41.7% of respondents showed that it should be raised to approximately $250,000/ha. In terms of land area restrictions, 88.0% of respondents believed that the minimum should be lowered to 0.4 ha. More than 70% of respondents owned less than 0.4 has of land, and since they do not own enough land to be eligible for the program, more than 80% of landlords wished to lower the minimum requirements of land area. In addition, 59.3% of respondents were reluctant to participate in reforestation because their lands were too small to be eligible; 15.0% of respondents were reluctant because the duration was too long. Responses to the question about “how the policy can be adjusted to provide incentives for landlords- participation" revealed that almost 40% of respondents desired higher subsidies. Some policy suggestions are provided as follows: (1) many landlords are still unaware of the GFP so the government should enhance the promotion of the policy; (2) many landlords are unwilling to participate in GFP mainly because they do not own enough lands to be eligible, hence the government should consider adjusting its requirements for minimum agricultural land area; (3) for subsequent promotions on GFP, the government may consider targeting on the landlords with high income and high level of education; (4) because the subsidy of this policy alone provides limited help to landlords, the government should help the landlords to explore other revenue possibilities from afforestation in addition to the existing subsidies and raise the participation incentives.
Abstract: Information is a critical asset and an important source for gaining competitive advantage in firms. The effective maintenance of IT becomes an important task. In order to better understand the determinants of IT effectiveness, this study employs the Industrial Organization (I/O) and Resource Based View (RBV) theories and investigates the industry effect and several major firmspecific factors in relation to their impact on firms- IT effectiveness. The data consist of a panel data of ten-year observations of firms whose IT excellence had been recognized by the CIO Magazine. The non-profit organizations were deliberately excluded, as explained later. The results showed that the effectiveness of IT management varied significantly across industries. Industry also moderated the effects of firm demographic factors such as size and age on IT effectiveness. Surprisingly, R & D investment intensity had negative correlation to IT effectiveness. For managers and practitioners, this study offers some insights for evaluation criteria and expectation for IT project success. Finally, the empirical results indicate that the sustainability of IT effectiveness appears to be short in duration.
Abstract: In this paper we examine some properties of suborbital graphs for the congruence subgroup r 0 (N) . Then we give necessary and sufficient conditions for graphs to have triangels.
Abstract: The research on the effectiveness of environmental
assessment (EA) is a milestone effort to evaluate the state of the field,
including many contributors related with a lot of countries since more
than two decades. In the 1960s, there was a surge of interest between
modern industrialized countries over unexpected opposite effects of
technical invention. The interest led to choice of approaches for
assessing and prediction the impressions of technology and
advancement for social and economic, state health and safety, solidity
and the circumstances. These are consisting of risk assessment,
technology assessment, environmental impact assessment and costbenefit
analysis. In this research contribution, the authors have
described the research status for environmental assessment in
cumulative environmental system. This article discusses the methods
for cumulative effect assessment (CEA).