Abstract: Atherosclerosis was identified as a chronic inflammatory process resulting from interactions between plasma lipoproteins, cellular components (monocyte, macrophages, T lymphocytes, endothelial cells and smooth muscle cells) and the extracellular matrix of the arterial wall. Several types of genes were known to express during formation of atherosclerosis. This study is carried out to identify unknown differentially expressed gene (DEG) in atherogenesis. Rabbit’s aorta tissues were stained by H&E for histomorphology. GeneFishing™ PCR analysis was performed from total RNA extracted from the aorta tissues. The DNA fragment from DEG was cloned, sequenced and validated by Real-time PCR. Histomorphology showed intimal thickening in the aorta. DEG detected from ACP-41 was identified as cathepsin B gene and showed upregulation at week-8 and week-12 of atherogenesis. Therefore, ACP-based GeneFishing™ PCR facilitated identification of cathepsin B gene which was differentially expressed during development of atherosclerosis.
Abstract: This paper presents a new method for estimating the mean curve of impulse voltage waveforms that are recorded during impulse tests. In practice, these waveforms are distorted by noise, oscillations and overshoot. The problem is formulated as an estimation problem. Estimation of the current signal parameters is achieved using a fast and accurate technique. The method is based on discrete dynamic filtering algorithm (DDF). The main advantage of the proposed technique is its ability in producing the estimates in a very short time and at a very high degree of accuracy. The algorithm uses sets of digital samples of the recorded impulse waveform. The proposed technique has been tested using simulated data of practical waveforms. Effects of number of samples and data window size are studied. Results are reported and discussed.
Abstract: Human genome is not only the evolutionary
summation of all advantageous events, but also houses lesions of
deleterious foot prints. A single gene mutation sometimes may
express multiple consequences in numerous tissues and a linear
relationship of the genotype and the phenotype may often be obscure.
ß Thalassemia minor, a transfusion independent mild anaemia,
coupled with environment among other factors may articulate into
phenotypic pleotropy with Hypocholesterolemia, Vitamin D
deficiency, Tissue hypoxia, Hyper-parathyroidism and Psychological
alterations. Occurrence of Pancreatic insufficiency, resultant
steatorrhoea, Vitamin-D (25-OH) deficiency (13.86 ngm/ml) with
Hypocholesterolemia (85mg/dl) in a 30 years old male ß Thal-minor
patient (Hemoglobin 11mg/dl with Fetal Hemoglobin 2.10%, Hb A2
4.60% and Hb Adult 84.80% and altered Hemogram) with increased
Para thyroid hormone (62 pg/ml) & moderate Serum Ca+2
(9.5mg/ml) indicate towards a cascade of phenotypic pleotropy
where the ß Thalassemia mutation ,be it in the 5’ cap site of the
mRNA , differential splicing etc in heterozygous state is effecting
several metabolic pathways. Compensatory extramedulary
hematopoiesis may not coped up well with the stressful life style of
the young individual and increased erythropoietic stress with high
demand for cholesterol for RBC membrane synthesis may have
resulted in Hypocholesterolemia.Oxidative stress and tissue hypoxia
may have caused the pancreatic insufficiency, leading to Vitamin D
deficiency. This may in turn have caused the secondary
hyperparathyroidism to sustain serum Calcium level. Irritability and
stress intolerance of the patient was a cumulative effect of the vicious
cycle of metabolic compromises. From these findings we propose
that the metabolic deficiencies in the ß Thalassemia mutations may
be considered as the phenotypic display of the pleotropy to explain
the genetic epidemiology.
According to the recommendations from the NIH Workshop on
Gene-Environment Interplay in Common Complex Diseases: Forging
an Integrative Model, study design of observations should be
informed by gene-environment hypotheses and results of a study
(genetic diseases) should be published to inform future hypotheses.
Variety of approaches is needed to capture data on all possible
aspects, each of which is likely to contribute to the etiology of
disease. Speakers also agreed that there is a need for development of
new statistical methods and measurement tools to appraise
information that may be missed out by conventional method where
large sample size is needed to segregate considerable effect.
A meta analytic cohort study in future may bring about significant
insight on to the title comment.
Abstract: This paper aims to study decomposition behavior in
pyrolytic environment of four lignocellulosic biomass (oil palm shell,
oil palm frond, rice husk and paddy straw), and two commercial
components of biomass (pure cellulose and lignin), performed in a
thermogravimetry analyzer (TGA). The unit which consists of a
microbalance and a furnace flowed with 100 cc (STP) min-1 Nitrogen,
N2 as inert. Heating rate was set at 20⁰C min-1 and temperature
started from 50 to 900⁰C. Hydrogen gas production during the
pyrolysis was observed using Agilent Gas Chromatography Analyzer
7890A. Oil palm shell, oil palm frond, paddy straw and rice husk
were found to be reactive enough in a pyrolytic environment of up to
900°C since pyrolysis of these biomass starts at temperature as low as
200°C and maximum value of weight loss is achieved at about
500°C. Since there was not much different in the cellulose,
hemicelluloses and lignin fractions between oil palm shell, oil palm
frond, paddy straw and rice husk, the T-50 and R-50 values obtained
are almost similar. H2 productions started rapidly at this temperature
as well due to the decompositions of biomass inside the TGA.
Biomass with more lignin content such as oil palm shell was found to
have longer duration of H2 production compared to materials of high
cellulose and hemicelluloses contents.
Abstract: In the upstream we place a piece of ring and rotate
it with 83Hz, 166Hz, 333Hz,and 666H to find the effect of the
periodic distortion.In the experiment this type of the perturbation
will not allow since the mechanical failure of any parts of the
equipment in the upstream will destroy the blade system. This type of
study will be only possible by CFD. We use two pumps NS32
(ENSAM) and three blades pump (Tamagawa Univ). The benchmark
computations were performed without perturbation parts, and confirm
the computational results well agreement in head-flow rate. We
obtained the pressure fluctuation growth rate that is representing the
global instability of the turbo-system. The fluctuating torque
components were 0.01Nm(5000rpm), 0.1Nm(10000rmp),
0.04Nm(20000rmp), 0.15Nm( 40000rmp) respectively. Only for
10000rpm(166Hz) the output toque was random, and it implies that it
creates unsteady flow by separations on the blades, and will reduce the
pressure loss significantly
Abstract: Leave of absence is important in maintaining a good
status of human resource quality. Allowing the employees temporarily
free from the routine assignments can vitalize the workers- morality
and productivity. This is particularly critical to secure a satisfactory
service quality for healthcare professionals of which were typically
featured with labor intensive and complicated works to perform. As
one of the veteran hospitals that were found and operated by the
Veteran Department of Taiwan, the nursing staff of the case hospital
was squeezed to an extreme minimum level under the pressure of a
tight budgeting. Leave of absence on schedule became extremely
difficult, especially for the intensive care units (ICU), in which
required close monitoring over the cared patients, and that had more
easily driven the ICU nurses nervous. Even worse, the deferred leaves
were more than 10 days at any time in the ICU because of a fluctuating
occupancy. As a result, these had brought a bad setback to this
particular nursing team, and consequently defeated the job
performance and service quality. To solve this problem and
accordingly to strengthen their morality, a project team was organized
across different departments specific for this. Sufficient information
regarding jobs and positions requirements, labor resources, and actual
working hours in detail were collected and analyzed in the team
meetings. Several alternatives were finalized. These included job
rotating, job combination, leave on impromptu and cross-departmental
redeployment. Consequently, the deferred leave days sharply reduced
70% to a level of 3 or less days. This improvement had not only
provided good shelter for the ICU nurses that improved their job
performance and patient safety but also encouraged the nurses active
participating of a project and learned the skills of solving problems
with colleagues.
Abstract: In wireless communication system, a Decision Feedback Equalizer (DFE) to cancel the intersymbol interference (ISI) is required. In this paper, an exact convergence analysis of the (DFE) adapted by the Least Mean Square (LMS) algorithm during the training phase is derived by taking into account the finite alphabet context of data transmission. This allows us to determine the shortest training sequence that allows to reach a given Mean Square Error (MSE). With the intention of avoiding the problem of ill-convergence, the paper proposes an initialization strategy for the blind decision directed (DD) algorithm. This then yields a semi-blind DFE with high speed and good convergence.
Abstract: Since the beginning of human history, human
activities have caused many changes in the environment. Today, a
particular attention should be paid to gaining knowledge about water
quality of wetlands which are pristine natural environments rich in
genetic reserves. If qualitative conditions of industrial areas (in terms
of both physicochemical and biological conditions) are not addressed
properly, they could cause disruption in natural ecosystems,
especially in rivers. With regards to the quality of water resources,
determination of pollutant sources plays a pivotal role in engineering
projects as well as designing water quality control systems. Thus,
using different methods such as flow duration curves, dischargepollution
load model and frequency analysis by HYFA software
package, risk of various industrial pollutants in international and
ecologically important Gavkhoni wetland is analyzed. In this study, a
station located at Varzaneh City is used as the last station on
Zayanderud River, from where the river water is discharged into the
wetland. Results showed that elements- concentrations often
exceeded the allowed level and river water can endanger regional
ecosystem. In addition, if the river discharge is managed on Q25
basis, this basis can lower concentrations of elements, keeping them
within the normal level.
Abstract: An electric power system includes a generating, a
transmission, a distribution, and consumers subsystems. An electrical
power network in Tanzania keeps growing larger by the day and
become more complex so that, most utilities have long wished for
real-time monitoring and remote control of electrical power system
elements such as substations, intelligent devices, power lines,
capacitor banks, feeder switches, fault analyzers and other physical
facilities. In this paper, the concept of automation of management of
power systems from generation level to end user levels was
determined by using Power System Simulator for Engineering
(PSS/E) version 30.3.2.
Abstract: Color image segmentation can be considered as a
cluster procedure in feature space. k-means and its adaptive
version, i.e. competitive learning approach are powerful tools
for data clustering. But k-means and competitive learning suffer
from several drawbacks such as dead-unit problem and need to
pre-specify number of cluster. In this paper, we will explore to
use competitive and cooperative learning approach to perform
color image segmentation. In competitive and cooperative
learning approach, seed points not only compete each other, but
also the winner will dynamically select several nearest
competitors to form a cooperative team to adapt to the input
together, finally it can automatically select the correct number
of cluster and avoid the dead-units problem. Experimental
results show that CCL can obtain better segmentation result.
Abstract: Solution to unsteady Navier-Stokes equation by Splitting method in physical orthogonal algebraic curvilinear coordinate system, also termed 'Non-linear grid system' is presented. The linear terms in Navier-Stokes equation are solved by Crank- Nicholson method while the non-linear term is solved by the second order Adams-Bashforth method. This work is meant to bring together the advantage of Splitting method as pressure-velocity solver of higher efficiency with the advantage of consuming Non-linear grid system which produce more accurate results in relatively equal number of grid points as compared to Cartesian grid. The validation of Splitting method as a solution of Navier-Stokes equation in Nonlinear grid system is done by comparison with the benchmark results for lid driven cavity flow by Ghia and some case studies including Backward Facing Step Flow Problem.
Abstract: This paper deals with the evaluation of flow properties
of polymeric matrix with natural animal fillers. Technical university
of Liberec cooperates on the long-term development of “green
materials“ that should replace conventionally used materials
(especially in automotive industry). Natural fibres (of animal and
plant origin) from all over the world are collected and adapted
(drying, cutting etc.) for extrusion processing. Inside the extruder
these natural additives are blended with polymeric (synthetic and
biodegradable - PLA) matrix and created compound is subsequently
cut for pellets in the wet way. These green materials with unique
recipes are then studied and their mechanical, physical and
processing properties are determined. The main goal of this research
is to develop new ecological materials very similar to unfilled
polymers. In this article the rheological behaviour of chosen natural
animal fibres is introduced considering their shape and surface that
were observed with use of SEM microscopy.
Abstract: Through a proper analysis of residual strain and stress
distributions obtained at the surface of high speed milled specimens
of AA 6082–T6 aluminium alloy, the performance of an improved
indentation method is evaluated. This method integrates a special
device of indentation to a universal measuring machine. The
mentioned device allows introducing elongated indents allowing to
diminish the absolute error of measurement. It must be noted that the
present method offers the great advantage of avoiding both the
specific equipment and highly qualified personnel, and their inherent
high costs. In this work, the cutting tool geometry and high speed
parameters are selected to introduce reduced plastic damage.
Through the variation of the depth of cut, the stability of the shapes
adopted by the residual strain and stress distributions is evaluated.
The results show that the strain and stress distributions remain
unchanged, compressive and small. Moreover, these distributions
reveal a similar asymmetry when the gradients corresponding to
conventional and climb cutting zones are compared.
Abstract: This paper presents the work of signal discrimination
specifically for Electrocardiogram (ECG) waveform. ECG signal is
comprised of P, QRS, and T waves in each normal heart beat to
describe the pattern of heart rhythms corresponds to a specific
individual. Further medical diagnosis could be done to determine any
heart related disease using ECG information. The emphasis on QRS
Complex classification is further discussed to illustrate the
importance of it. Pan-Tompkins Algorithm, a widely known
technique has been adapted to realize the QRS Complex
classification process. There are eight steps involved namely
sampling, normalization, low pass filter, high pass filter (build a band
pass filter), derivation, squaring, averaging and lastly is the QRS
detection. The simulation results obtained is represented in a
Graphical User Interface (GUI) developed using MATLAB.
Abstract: Bioinformatics and Cheminformatics use computer as disciplines providing tools for acquisition, storage, processing, analysis, integrate data and for the development of potential applications of biological and chemical data. A chemical database is one of the databases that exclusively designed to store chemical information. NMRShiftDB is one of the main databases that used to represent the chemical structures in 2D or 3D structures. SMILES format is one of many ways to write a chemical structure in a linear format. In this study we extracted Antimicrobial Structures in SMILES format from NMRShiftDB and stored it in our Local Data Warehouse with its corresponding information. Additionally, we developed a searching tool that would response to user-s query using the JME Editor tool that allows user to draw or edit molecules and converts the drawn structure into SMILES format. We applied Quick Search algorithm to search for Antimicrobial Structures in our Local Data Ware House.
Abstract: Yogurt is a coagulated milk product obtained from
the lactic acid fermentation by the action of Lactobacillus
bulgaricus and Streptococcus thermophilus. The additions of fruits
into milk may enhance the taste and the therapeutical values of milk
products. However fruits also may change the fermentation
behaviour. In this present study, the changes in physicochemical, the
peptide concentration, total phenolics content and the antioxidant
potential of yogurt upon the addition of Hylocereus polyrhizus and
Hylocereus undatus (white and red dragon fruit) were investigated.
Fruits enriched yogurt (10%, 20%, 30% w/w) were prepared and the
pH, TTA, syneresis measurement, peptide concentration, total
phenolics content and DPPH antioxidant inhibition percentage were
determined. Milk fermentation rate was enhanced in red dragon fruit
yogurt for all doses (-0.3606 - -0.4126 pH/h) while only white
dragon fruit yogurt with 20% and 30% (w/w) composition showed
increment in fermentation rate (-0.3471 - -0.3609 pH/h) compared to
plain yogurt (-0.3369pH/h). All dragon fruit enriched yogurts
generally showed lower pH readings (pH 3.95 - 4.03) compared to
plain yogurt (pH 4.05). Both fruit yogurts showed a higher lactic
acid percentage (1.14-1.23%) compared to plain yogurt (1.08%).
Significantly higher syneresis percentage (57.19 - 70.32%)
compared to plain yogurt (52.93%) were seen in all fruit enriched
yogurts. The antioxidant activity of plain yogurt (19.16%) was
enhanced by the presence of white and red dragon fruit (24.97-
45.74%). All fruit enriched yogurt showed an increment in total
phenolic content (36.44 - 64.43mg/ml) compared to plain yogurt
(20.25mg/ml). However, the addition of white and red dragon fruit
did not enhance the proteolysis of milk during fermentation.
Therefore, it could be concluded that the addition of white and red
dragon fruit into yogurt enhanced the milk fermentation rate, lactic
acid content, syneresis percentage, antioxidant activity, and total
phenolics content in yogurt.
Abstract: This study was designed to investigate the role of serum nitric oxide and sialic acid in the development of diabetic nephropathy as disease marker. Total 210 diabetic patients (age and sex matched) were selected followed by informed consent and divided into four groups (70 each) as I: control; II: diabetic; III: diabetic hypertensive; IV: diabetic nephropathy. The blood samples of all subjects were collected and analyzed for serum nitric oxide, sialic acid, fasting blood glucose, serum urea, creatinine, HbA1c and GFR. The BMI, systolic and diastolic blood pressures, blood glucose, HbA1c and serum sialic acid levels were high (p
Abstract: Organochlorine pesticides (OCPs) are known to be
persistent and bioaccumulative toxicants that may cause reproductive
impairments in wildlife as well as human. The current study uses the
snail-eating turtle Malayemys macrocephala, a long-lived animal
commonly distribute in rice field habitat in central part of Thailand,
as a sentinel to monitor OCP contamination in environment. The
nest soil, complete clutch of eggs, and blood of the turtle were
collected from agricultural areas in the Chao Phraya River Basin,
Thailand during the nesting season of 2007-2008. The novel
methods for tissue extraction by an accelerated solvent extractor
(ASE, for egg) and liquid-liquid extraction (for blood) have been
developed. The nineteen OCP residues were analyzed by gas
chromatography with micro-electron captured detector (GC-μECD).
The validated methods have met requirements of the AOAC
standard. The results indicated that significant amounts of OCPs are
still contaminated in nest soil and eggs of the turtle even though the
OCPs had been banned in this area for many years. This suggested
the potential risk to health of wildlife as well as human in the area.
Abstract: This paper deals with the problem of thermal and
mechanical shocks, which rising during operation, mostly at
interrupted cut. Here will be solved their impact on the cutting edge
tool life, the impact of coating technology on resistance to shocks
and experimental determination of tool life in heating flame.
Resistance of removable cutting edges against thermal and
mechanical shock is an important indicator of quality as well as its
abrasion resistance. Breach of the edge or its crumble may occur due
to cyclic loading. We can observe it not only during the interrupted
cutting (milling, turning areas abandoned hole or slot), but also in
continuous cutting. This is due to the volatility of cutting force on
cutting. Frequency of the volatility in this case depends on the type
of rising chips (chip size element). For difficult-to-machine materials
such as austenitic steel particularly happened at higher cutting speeds
for the localization of plastic deformation in the shear plane and for
the inception of separate elements substantially continuous chips.
This leads to variations of cutting forces substantially greater than for
other types of steel.
Abstract: In this article we present a methodology which
enables preschool and primary school unlanguaged children to
remember words, phrases and texts with the help of graphic signs -
letters, syllables and words. Reading for a child becomes a support
for speech development. Teaching is based on the principle "from
simple to complex", "a letter - a syllable - a word - a proposal - a
text." Availability of multi-level texts allows using this methodology
for working with children who have different levels of speech
development.