Abstract: The DNA microarray technology concurrently monitors the expression levels of thousands of genes during significant biological processes and across the related samples. The better understanding of functional genomics is obtained by extracting the patterns hidden in gene expression data. It is handled by clustering which reveals natural structures and identify interesting patterns in the underlying data. In the proposed work clustering gene expression data is done through an Advanced Nelder Mead (ANM) algorithm. Nelder Mead (NM) method is a method designed for optimization process. In Nelder Mead method, the vertices of a triangle are considered as the solutions. Many operations are performed on this triangle to obtain a better result. In the proposed work, the operations like reflection and expansion is eliminated and a new operation called spread-out is introduced. The spread-out operation will increase the global search area and thus provides a better result on optimization. The spread-out operation will give three points and the best among these three points will be used to replace the worst point. The experiment results are analyzed with optimization benchmark test functions and gene expression benchmark datasets. The results show that ANM outperforms NM in both benchmarks.
Abstract: To enhance installation security, a LNG storage in Rudong of Jiangsu province was adopted as a practical work, and it was analyzed by nonlinear finite element method to research overall and local stability performance, as well as the stress and deformation under the action of wind load and self-weight. Results indicate that deformation is tiny when steel mesh maintains as an overall ring, and stress caused by vertical bending moment and tension of bottom tie wire are also in the safe range. However, axial forces of lap reinforcement in adjacent steel mesh exceed the ultimate bearing capacity of tie wire. Hence, tie wires are ruptured; single mesh loses lateral connection and turns into monolithic status as the destruction of overall structure. Further more, monolithic steel mesh is led to collapse by the damage of bottom connection. So, in order to prevent connection failure and enhance installation security, the overlapping parts of steel mesh should be taken more reliable measures.
Abstract: Creation and maintenance of knowledge management
systems has been recognized as an important research area.
Consecutively lack of accurate results from knowledge management
systems limits the organization to apply their knowledge
management processes. This leads to a failure in getting the right
information to the right people at the right time thus followed by a
deficiency in decision making processes. An Intranet offers a
powerful tool for communication and collaboration, presenting data
and information, and the means that creates and shares knowledge,
all in one easily accessible place. This paper proposes an archetype
describing how a knowledge management system, with the support
of intranet capabilities, could very much increase the accuracy of
capturing, storing and retrieving knowledge based processes thereby
increasing the efficiency of the system. This system will expect a
critical mass of usage, by the users, for intranet to function as
knowledge management systems. This prototype would lead to a
design of an application that would impose creation and maintenance
of an effective knowledge management system through intranet. The
aim of this paper is to introduce an effective system to handle
capture, store and distribute knowledge management in a form that
may not lead to any failure which exists in most of the systems. The
methodology used in the system would require all the employees, in
the organization, to contribute the maximum to deliver the system to
a successful arena. The system is still in its initial mode and thereby
the authors are under the process to practically implement the ideas,
as mentioned in the system, to produce satisfactory results.
Abstract: Electrical discharge machining (EDM) is well
established machining technique mainly used to machine complex
geometries on difficult-to-machine materials and high strength
temperature resistant alloys. In the present research, the objective is
to study the shape of the electrode and establish the application of
liquid nitrogen in reducing distortion of the electrode during
electrical discharge machining of M2 grade high speed steel using
copper electrodes. Study of roundness was performed on the
electrode to observe the shape of the electrode for both conventional
EDM and EDM with cryogenically cooled electrode. Scanning
Electron Microscope (SEM) has been used to study the shape of
electrode tip. The effect of various parameters such as discharge
current and pulse on time has been studied to understand the behavior
of distortion of electrode. It has been concluded that the shape
retention is better in case of liquid nitrogen cooled electrode.
Abstract: Collaborative planning, forecasting and
replenishment (CPFR) coordinates the various supply chain
management activities including production and purchase planning,
demand forecasting and inventory replenishment between supply
chain trading partners. This study proposes a systematic way of
analyzing CPFR supporting factors using fuzzy cognitive map
(FCM) approach. FCMs have proven particularly useful for solving
problems in which a number of decision variables and
uncontrollable variables are causally interrelated. Hence the FCMs
of CPFR are created to show the relationships between the factors
that influence on effective implementation of CPFR in the supply
chain.
Abstract: Mammalian genomes contain large number of
retroelements (SINEs, LINEs and LTRs) which could affect
expression of protein coding genes through associated transcription
factor binding sites (TFBS). Activity of the retroelement-associated
TFBS in many genes is confirmed experimentally but their global
functional impact remains unclear. Human SINEs (Alu repeats) and
mouse SINEs (B1 and B2 repeats) are known to be clustered in GCrich
gene rich genome segments consistent with the view that they
can contribute to regulation of gene expression. We have shown
earlier that Alu are involved in formation of cis-regulatory modules
(clusters of TFBS) in human promoters, and other authors reported
that Alu located near promoter CpG islands have an increased
frequency of CpG dinucleotides suggesting that these Alu are
undermethylated. Human Alu and mouse B1/B2 elements have an
internal bipartite promoter for RNA polymerase III containing
conserved sequence motif called B-box which can bind basal
transcription complex TFIIIC. It has been recently shown that TFIIIC
binding to B-box leads to formation of a boundary which limits
spread of repressive chromatin modifications in S. pombe. SINEassociated
B-boxes may have similar function but conservation of
TFIIIC binding sites in SINEs located near mammalian promoters
has not been studied earlier. Here we analysed abundance and
distribution of retroelements (SINEs, LINEs and LTRs) in annotated
sequences of the Database of mammalian transcription start sites
(DBTSS). Fractions of SINEs in human and mouse promoters are
slightly lower than in all genome but >40% of human and mouse
promoters contain Alu or B1/B2 elements within -1000 to +200 bp
interval relative to transcription start site (TSS). Most of these SINEs
is associated with distal segments of promoters (-1000 to -200 bp
relative to TSS) indicating that their insertion at distances >200 bp
upstream of TSS is tolerated during evolution. Distribution of SINEs
in promoters correlates negatively with the distribution of CpG
sequences. Using analysis of abundance of 12-mer motifs from the
B1 and Alu consensus sequences in genome and DBTSS it has been
confirmed that some subsegments of Alu and B1 elements are poorly
conserved which depends in part on the presence of CpG
dinucleotides. One of these CpG-containing subsegments in B1
elements overlaps with SINE-associated B-box and it shows better
conservation in DBTSS compared to genomic sequences. It has been
also studied conservation in DBTSS and genome of the B-box
containing segments of old (AluJ, AluS) and young (AluY) Alu
repeats and found that CpG sequence of the B-box of old Alu is
better conserved in DBTSS than in genome. This indicates that Bbox-
associated CpGs in promoters are better protected from
methylation and mutation than B-box-associated CpGs in genomic
SINEs. These results are consistent with the view that potential
TFIIIC binding motifs in SINEs associated with human and mouse
promoters may be functionally important. These motifs may protect
promoters from repressive histone modifications which spread from
adjacent sequences. This can potentially explain well known
clustering of SINEs in GC-rich gene rich genome compartments and
existence of unmethylated CpG islands.
Abstract: This paper features the trajectory planning design of a indigenously developed 4-Axis SCARA robot which is used for doing successful robotic manipulation task in the laboratory. Once, a trajectory is being designed and given as input to the robot, the robot's gripper tip moves along that specified trajectory. Trajectories have to be designed in the work space only. The main idea of this paper is to design a continuous path trajectory model for the indigenously developed SCARA robot arm during its maneuvering from one point to another point (during pick and place operations) in a workspace avoiding all the obstacles in its path of motion.
Abstract: This paper proposes an adaptive sliding mode
controller which combines adaptive control and sliding
mode control to control a nonlinear robotic manipulator
with uncertain parameters. We use an adaptive algorithm
based on the concept of sliding mode control to alleviate the
chattering phenomenon of control input. Adaptive laws are
developed to obtain the gain of switching input and the
boundary layer parameters. The stability and convergence
of the robotic manipulator control system are guaranteed
by applying the Lyapunov theorem. Simulation results
demonstrate that the chattering of control input can be
alleviated effectively. The proposed controller scheme can
assure robustness against a large class of uncertainties and
achieve good trajectory tracking performance.
Abstract: Web-based systems have become increasingly
important due to the fact that the Internet and the World Wide Web
have become ubiquitous, surpassing all other technological
developments in our history. The Internet and especially companies
websites has rapidly evolved in their scope and extent of use, from
being a little more than fixed advertising material, i.e. a "web
presences", which had no particular influence for the company's
business, to being one of the most essential parts of the company's
core business.
Traditional software engineering approaches with process models
such as, for example, CMM and Waterfall models, do not work very
well since web system development differs from traditional
development. The development differs in several ways, for example,
there is a large gap between traditional software engineering designs
and concepts and the low-level implementation model, many of the
web based system development activities are business oriented (for
example web application are sales-oriented, web application and
intranets are content-oriented) and not engineering-oriented.
This paper aims to introduce Increment Iterative extreme
Programming (IIXP) methodology for developing web based
systems. In difference to the other existence methodologies, this
methodology is combination of different traditional and modern
software engineering and web engineering principles.
Abstract: We present a preliminary x-ray study on human-hair
microstructures for a health-state indicator, in particular a cancer
case. As an uncomplicated and low-cost method of x-ray technique,
the human-hair microstructure was analyzed by wide-angle x-ray
diffractions (XRD) and small-angle x-ray scattering (SAXS). The
XRD measurements exhibited the simply reflections at the d-spacing
of 28 Å, 9.4 Å and 4.4 Å representing to the periodic distance of the
protein matrix of the human-hair macrofibrous and the diameter and
the repeated spacing of the polypeptide alpha helixes of the
photofibrils of the human-hair microfibrous, respectively. When
compared to the normal cases, the unhealthy cases including to the
breast- and ovarian-cancer cases obtained higher normalized ratios of
the x-ray diffracting peaks of 9.4 Å and 4.4 Å. This likely resulted
from the varied distributions of microstructures by a molecular
alteration. As an elemental analysis by x-ray fluorescence (XRF), the
normalized quantitative ratios of zinc(Zn)/calcium(Ca) and
iron(Fe)/calcium(Ca) were determined. Analogously, both Zn/Ca and
Fe/Ca ratios of the unhealthy cases were obtained higher than both of
the normal cases were. Combining the structural analysis by XRD
measurements and the elemental analysis by XRF measurements
exhibited that the modified fibrous microstructures of hair samples
were in relation to their altered elemental compositions. Therefore,
these microstructural and elemental analyses of hair samples will be
benefit to associate with a diagnosis of cancer and genetic diseases.
This functional method would lower a risk of such diseases by the
early diagnosis. However, the high-intensity x-ray source, the highresolution
x-ray detector, and more hair samples are necessarily
desired to develop this x-ray technique and the efficiency would be
enhanced by including the skin and fingernail samples with the
human-hair analysis.
Abstract: Pure phase gallosilicate nitrite sodalite has been synthesized in a single step by low temperature (373 oK) hydrothermal technique. The product obtained was characterized using a combination of techniques including X-ray powder diffraction, IR, Raman spectroscopy, SEM, MAS NMR spectroscopy as well as thermogravimetry. Sodalite with an ideal composition was obtained after synthesis at 3730K and seven days duration using alkaline medium. The structural features of the Na8[GaSiO4]6(NO2)2 sodalite were investigated by IR, MAS NMR spectroscopy of 29Si and 23Na nuclei and by Reitveld refinement of X-ray powder diffraction data. The crystal structure of this sodalite has been refined in the space group P 4 3n; with a cell parameter 8.98386Å, V= 726.9 Å, (Rwp= 0.077 and Rp=0.0537) and Si-O-Ga angle is found to be 132.920 . MAS NMR study confirms complete ordering of Si and Ga in the gallosilicate framework. The surface area of single entity with stoichiometry Na8[GaSiO4]6(NO2)2 was found to be 8.083 x10-15 cm2/g.
Abstract: In mammalian reproductive tract, the oviduct secretes
huge number of growth factors and cytokines that create an optimal
micro-environment for the initial stages of preimplantation embryos.
Secretion of these growth factors is stage-specific. Among them,
VEGF is a potent mitogen for vascular endothelium and stimulates
vascular permeability. Apart from angiogenesis, VEGF in the oviduct
may be involved in regulating the oocyte maturation and subsequent
developmental process during embryo production in vitro. In
experiment 1, to evaluate the effect of VEGF during IVM of porcine
COC and subsequent developmental ability after PA and SCNT. The
results from these experiments indicated that maturation rates among
the different VEGF concentrations were not significant different. In
experiment 2, total intracellular GSH concentrations of oocytes
matured with VEGF (5-50 ng/ml) were increased significantly
compared to a control and VEGF group (500 ng/ml). In experiment 3,
the blastocyst formation rates and total cell number per blastocyst
after parthenogenesis of oocytes matured with VEGF (5-50 ng/ml)
were increased significantly compared to a control and VEGF group
(500 ng/ml). Similarly, in experiment 4, the blastocyst formation rate
and total cell number per blastocyst after SCNT and IVF of oocytes
matured with VEGF (5 ng/ml) were significantly higher than that of
oocytes matured without VEGF group. In experiment 5, at 10 hour
after the onset of IVF, pronuclear formation rate was evaluated.
Monospermy was significantly higher in VEGF-matured oocytes than
in the control, and polyspermy and sperm penetration per oocyte
were significantly higher in the control group than in the VEGFmatured
oocytes. Supplementation with VEGF during IVM
significantly improved male pronuclear formation as compared with
the control. In experiment 6, type III cortical granule distribution in
oocytes was more common in VEGF-matured oocytes than in the
control. In conclusion, the present study suggested that
supplementation of VEGF during IVM may enhance the
developmental potential of porcine in vitro embryos through increase
of the intracellular GSH level, higher MPN formation and increased
fertilization rate as a consequence of an improved cytoplasmic
maturation.
Abstract: In this paper, an magnetorheological (MR) mount with
fuzzy sliding mode controller (FSMC) is studied for vibration
suppression when the system is subject to base excitations. In recent
years, magnetorheological fluids are becoming a popular material in
the field of the semi-active control. However, the dynamic equation of
an MR mount is highly nonlinear and it is difficult to identify. FSMC
provides a simple method to achieve vibration attenuation of the
nonlinear system with uncertain disturbances. This method is capable
of handling the chattering problem of sliding mode control effectively
and the fuzzy control rules are obtained by using the Lyapunov
stability theory. The numerical simulations using one-dimension and
two-dimension FSMC show effectiveness of the proposed controller
for vibration suppression. Further, the well-known skyhook control
scheme and an adaptive sliding mode controller are also included in
the simulation for comparison with the proposed FSMC.
Abstract: Prediction of sinusoidal signals with time-varying
frequencies has been an important research topic in power electronics
systems. To solve this problem, we propose a new fuzzy
predictive filtering scheme, which is based on a Finite Impulse
Response (FIR) filter bank. Fuzzy logic is introduced here to provide
appropriate interpolation of individual filter outputs. Therefore,
instead of regular 'hard' switching, our method has the advantageous
'soft' switching among different filters. Simulation
comparisons between the fuzzy predictive filtering and conventional
filter bank-based approach are made to demonstrate that the
new scheme can achieve an enhanced prediction performance for
slowly changing sinusoidal input signals.
Abstract: The research was designed to examine the relationship
between the development of muscle fatigue and the effect it has on
sport performance, specifically during maximal voluntary
contraction. This kind of this investigation using simultaneous
electrophysiological and mechanical recordings, based on advanced
mathematical processing, allows us to get parameters, and indexes in
a short time, and finally, the mapping to use for the thorough
investigation of the muscle contraction force, respectively the
phenomenon of local muscle fatigue, both for athletes and other
subjects.
Abstract: This paper presents an optimal and unsupervised satellite image segmentation approach based on Pearson system and k-Means Clustering Algorithm Initialization. Such method could be considered as original by the fact that it utilised K-Means clustering algorithm for an optimal initialisation of image class number on one hand and it exploited Pearson system for an optimal statistical distributions- affectation of each considered class on the other hand. Satellite image exploitation requires the use of different approaches, especially those founded on the unsupervised statistical segmentation principle. Such approaches necessitate definition of several parameters like image class number, class variables- estimation and generalised mixture distributions. Use of statistical images- attributes assured convincing and promoting results under the condition of having an optimal initialisation step with appropriated statistical distributions- affectation. Pearson system associated with a k-means clustering algorithm and Stochastic Expectation-Maximization 'SEM' algorithm could be adapted to such problem. For each image-s class, Pearson system attributes one distribution type according to different parameters and especially the Skewness 'β1' and the kurtosis 'β2'. The different adapted algorithms, K-Means clustering algorithm, SEM algorithm and Pearson system algorithm, are then applied to satellite image segmentation problem. Efficiency of those combined algorithms was firstly validated with the Mean Quadratic Error 'MQE' evaluation, and secondly with visual inspection along several comparisons of these unsupervised images- segmentation.
Abstract: Generalization is one of the most challenging issues
of Learning Classifier Systems. This feature depends on the
representation method which the system used. Considering the
proposed representation schemes for Learning Classifier System, it
can be concluded that many of them are designed to describe the
shape of the region which the environmental states belong and the
other relations of the environmental state with that region was
ignored. In this paper, we propose a new representation scheme
which is designed to show various relationships between the
environmental state and the region that is specified with a particular
classifier.
Abstract: In the present study, computational fluid dynamics
(CFD) simulation has been executed to investigate the transition
boundaries of different flow patterns for moderately viscous oil-water
(viscosity ratio 107, density ratio 0.89 and interfacial tension of 0.032
N/m.) two-phase flow through a horizontal pipeline with internal
diameter and length of 0.025 m and 7.16 m respectively. Volume of
Fluid (VOF) approach including effect of surface tension has been
employed to predict the flow pattern. Geometry and meshing of the
present problem has been drawn using GAMBIT and ANSYS
FLUENT has been used for simulation. A total of 47037 quadrilateral
elements are chosen for the geometry of horizontal pipeline. The
computation has been performed by assuming unsteady flow,
immiscible liquid pair, constant liquid properties, co-axial flow and a
T-junction as entry section. The simulation correctly predicts the
transition boundaries of wavy stratified to stratified mixed flow.
Other transition boundaries are yet to be simulated. Simulated data
has been validated with our own experimental results.
Abstract: Mycophenolic acid (MPA) is a secondary metabolite
produced by Penicillium brevicompactum, which has antibiotic and
immunosuppressive properties. In this study, the first, mycophenolic
acid was produced in a fermentation process by Penicillium
brevicompactum MUCL 19011 in shake flask using a base medium.
The maximum MPA production, product yield and productivity of
process were 1.379 g/L, 18.6 mg/g glucose and 4.9 mg/L. h,
respectively. Also the glucose consumption, biomass and MPA
production profiles were investigated during batch cultivation.
Obtained results showed that MPA production starts approximately
after 180 hours and reaches to a maximum at 280 h. In the next step,
the effects of some various concentrations of enzymatically
hydrolyzed casein on MPA production were evaluated. Maximum
MPA production, product yield and productivity as 3.63 g/L, 49
mg/g glucose and 12.96 mg/L.h, respectively were obtained with
using 30 g/L enzymatically hydrolyzed casein in culture medium.
These values show an enhanced MPA production, product yield and
process productivity pr as 116.8%, 132.8% and 163.2%, respectively.
Abstract: Measuring the effect of perinatal lead exposure on learning ability of offspring is considered as a sensitive and selective index for providing an early marker for central nervous system damage produced by this toxic metal. A total of 35 Sprague-Dawley adult rats were used to investigate the effect of lead acetate toxicity on behavioral patterns of adult female rats and learning ability of offspring. Rats were allotted into 4 groups, group one received 1g/l lead acetate (n=10), group two received 1.5g/l lead acetate (n=10), group three received 2g/l lead acetate in drinking water (n=10) and control group did not receive lead acetate (n=5) from 8th day of pregnancy till weaning of pups.
The obtained results revealed a dose dependent increase in the feeding time, drinking frequency, licking frequency, scratching frequency, licking litters, nest building and retrieving frequencies, while standing time increased significantly in rats treated with 1.5g/l lead acetate than other treated groups and control, on contrary lying time decreased gradually in a dose dependent manner. Moreover, movement activities were higher in rats treated with 1g/l lead acetate than other treated groups and control. Furthermore, time spent in closed arms was significantly lower in rats given 2g/l lead acetate than other treated groups, while, they spent significantly much time spent in open arms than other treated groups which could be attributed to occurrence of adaptation. Furthermore, number of entries in open arms was dose dependent. However, the ratio between open/closed arms revealed a significant decrease in rats treated with 2g/l lead acetate than control group.