Abstract: The aim of the present work was to statistically design
an autotrophic medium for maximum biomass production by
Chlorella pyrenoidosa using response surface methodology. After
evaluating one factor at a time approach, K2HPO4, KNO3,
MgSO4.7H2O and NaHCO3 were preferred over the other
components of the fog’s medium as most critical autotrophic medium
components. The study showed that the maximum biomass yield was
achieved while the concentrations of MgSO4.7H2O, K2HPO4, KNO3
and NaHCO3 were 0.409 g/L, 0.24 g/L, 1.033 g/L, and 3.265 g/L,
respectively. The study reported that the biomass productivity of C.
pyrenoidosa improved from 0.14 g/L in defined fog’s medium to 1.40
g/L in modified fog’s medium resulting 10 fold increase. The
biochemical composition biosynthesis of C. pyrenoidosa was altered
using nitrogen limiting stress bringing about 5.23 fold increase in
lipid content than control (cell without stress), as analyzed by FTIR
integration method.
Abstract: Objective of this study is to explore the recent trends,
patterns and the structural changes in the labour migration from Sri
Lanka to Middle East countries and to discuss the possible impacts of
those changes on the remittance flow. Study uses secondary data
published by Sri Lanka Bureau of Foreign Employment and Central
Bank. Thematic analysis of the secondary data revealed that the
migration for labour has increased rapidly during past decades.
Parallel with that the gender and the skill composition of the
migration flow has been changing. Similarly, the destinations for
male migration have changed over the period. These show positive
implications on the international remittance receipts to the country.
Abstract: Since the marine environmental conditions are
extremely different from the other ones, marine actinomycetes might
produce novel bioactive compounds. Therefore, actinomycete strains
were screened from marine water and sediment samples collected
from the coastal areas of Northern Vietnam. Ninety-nine
actinomycete strains were obtained on starch-casein agar media by
dilution technique, only seven strains, named HP112, HP12, HP411,
HPN11, HP 11, HPT13 and HPX12, showed significant antibacterial
activity against both gram-positive and gram-negative bacteria
(Bacillus subtilis ATCC 6633, Staphylococcus epidemidis ATCC
12228, Escherichia coli ATCC 11105). Further studies were carried
out with the most active HP411 strain against Candida albicans
ATCC 10231. This strain could grow rapidly on starch casein agar
and other media with high salt containing 7-10% NaCl at 28-30oC.
Spore-chain of HP411 showed an elongated and circular shape with
10 to 30 spores/chain. Identification of the strain was carried out by
employing the taxonomical studies including the 16S rRNA
sequence. Based on phylogenetic and phenotypic evidence it is
proposed that HP411 to be belongs to species Streptomyces
variabilis. The potent of the crude extract of fermentation broth of
HP411 that are effective against wide range of pathogens: both grampositive,
gram-negative and fungi. Further studies revealed that the
crude extract HP411 could obtain the anticancer activity for cancer
cell lines: Hep-G2 (liver cancer cell line); RD (cardiac and skeletal
muscle letters cell line); FL (membrane of the uterus cancer cell line).
However, the actinomycetes from marine ecosystem will be useful
for the discovery of new drugs in the future.
Abstract: Food contamination occurs during post process
handling. This leads to spoilage and growth of pathogenic
microorganisms in the food, thereby reducing its shelf life or
spreading of food borne diseases. Several methods are tried and one
of which is use of antimicrobial packaging. Here, papain, a protease
enzyme, is covalently immobilized with the help of glutarldehyde on
polyurethane and used as a food wrap to protect food from microbial
contamination. Covalent immobilization of papain was achieved at a
pH of 7.4; temperature of 4°C; glutaraldehyde concentration of 0.5%;
incubation time of 24h; and 50mg of papain. The formation of -C=Nobserved
in the Fourier transform infrared spectrum confirmed the
immobilization of the enzyme on the polymer. Immobilized enzyme
retained higher activity than the native free enzyme. The modified
polyurethane showed better reduction of Staphylococcus aureus
biofilm than bare polymer film (eight folds reduction in live colonies,
two times reduction in protein and 6 times reduction in
carbohydrates). The efficacy of this was studied by wrapping it over
S. aureus contaminated cottage cheese (paneer) and cheese and
stored at a temperature of 4°C for 7days. The modified film reduced
the bacterial contamination by eight folds when compared to the bare
film. FTIR also indicated reduction in lipids, sugars and proteins in
the biofilm.
Abstract: This paper explains about the voltage output for DC to
DC boost converter between open loop, PID controller and fuzzy
logic controller through Matlab Simulink. Simulink input voltage was
set at 12V and the voltage reference was set at 24V. The analysis on
the deviation of voltage resulted that the difference between reference
voltage setting and the output voltage is always lower. Comparison
between open loop, PID and FLC shows that, the open loop circuit
having a bit higher on the deviation of voltage. The PID circuit
boosts for FLC has a lesser deviation of voltage and proved that it is
such a better performance on control the deviation of voltage during
the boost mode.
Abstract: The effective development of a geoscience education
and training program takes account of the rapidly changing
environment in the geoscience market, includes information about
resource-rich countries which have international education demands.
In this paper, we introduce the geoscience program run by the
International School for Geoscience Resources at the Korea Institute
of Geoscience and Mineral Resources (IS-Geo of KIGAM), and show
its remarkable performance. To further effective geoscience program
planning and operation, we present recommendations for strategic
management for customer-oriented operation with a more favorable
program format and advanced training aids. Above all, the IS-Geo of
KIGAM should continue improve through ‘plan-do-see-feedback’
activities based on the recommendations.
Abstract: The aim of present study was to monitor the presence
of Trichodina sp. in Rainbow trout, Oncorhynchus mykiss collected
from various fish farms in the western provinces of Iran during
January, 2013- January, 2014. Out of 675 sampled fish 335, (49.16%)
were infested with Trichodina. The highest prevalence was observed
in the spring and winter followed by autumn and summer. In general,
the intensity of infection was low except in cases where outbreaks of
Trichodiniasis endangered the survival of fish in some ponds. In light
infestation Trichodina is usually present on gills, fins and skin of
apparently healthy fish. Clinical signs of Trichodiniasis only appear
on fish with heavy infections and cases of moderate ones that are
usually exposed to one or more stress factors including, rough
handling during transportation from ponds, overcrowdness,
malnutrition, high of free ammonia and low of oxygen concentration.
Clinical signs of Trichodiniasis in sampled fish were sluggish
movement, loss of appetite, black coloration, necrosis and ulcer on
different parts of the body, detached scales and excessive
accumulation of mucous in gill pouches. The most obvious
histopathological changes in diseased fish were sloughing of the
epidermal layer, aggregation of leucocytes and melanine-carrying
cells (between the dermis and hypodermis) and proliferative changes
including hyperplasia and hypertrophy of the epithelial lining cells of
gill filaments which resulted in fusion of secondary lamellae. Control
of Trichodiniasis, has been achieved by formalin bath treatment at a
concentration of 250 ppm for one hour.
Abstract: Urban greenery remains the bastion of urban
landscape and a key to sustainable development due to its integral
connections to the general health and wellbeing of urban residents.
However, in an era of rapid urbanisation, recent studies indicate that
urban greenery, especially ecologically sensitive areas, in many
African cities is becoming increasingly depleted. Given the scale and
rate of natural and anthropogenic change, effective management of
urban greenery as the ultimate goal of restoring depleting urban
landscapes is urgent. This review advocates for an urban resilience
model to managing urban greenery.
Abstract: Ongoing landscape transformation is one of the major
causes behind disappearance of traditional landscapes, and lead to
species and resource loss. Tree in paddy fields in the northeast of
Thailand is one of those traditional landscapes. Using three different
historical time layers, we acknowledged the severe deforestation and
rapid urbanization happened in the region. Despite the general
thinking of decline in tree density as consequences, the heterogeneous
trend of changes in total tree density in three studied landscapes denied
the hypothesis that number of trees in paddy field depend on the length
of land use practice. On the other hand, due to selection of planting
new trees on levees, existence of trees in paddy field now relies on
their values for human use. Besides, changes in land use and landscape
structure had a significant impact on decision of which tree density
level is considered as suitable for the landscape.
Abstract: Calcium phosphate coating (CaP) has been employed
for protein delivery, but the typical direct protein adsorption on the
coating led to low incorporation content and fast release of the
protein from the coating. By using bovine serum albumin (BSA) as a
model protein, rapid biomimetic co-precipitation between calcium
phosphate and BSA was employed to control the distribution of BSA
within calcium phosphate coating during biomimetic formation on
titanium surface for only 6 h at 50oC in an accelerated calcium
phosphate solution. As a result, the amount of BSA incorporation and
release duration could be increased by using a rapid biomimetic coprecipitation
technique. Up to 43 fold increases in the BSA
incorporation content and the increase from 6 h to more than 360 h in
release duration compared to typical direct adsorption technique were
observed depending on the initial BSA concentration used during coprecipitation
(1, 10 and 100 μg.ml-1). From x-ray diffraction and
Fourier transform infrared spectroscopy studies, the coating
composition was not altered with the incorporation of BSA by this
rapid biomimetic co-precipitation and mainly comprised octacalcium
phosphate and hydroxyapatite. However, the microstructure of
calcium phosphate crystals changed from straight, plate-like units to
curved, plate-like units with increasing BSA content.
Abstract: With demand for primary energy continuously
growing, search for renewable and efficient energy sources has been
high on agenda of our society. One of the most promising energy
sources is biogas technology. Residues coming from dairy industry
and milk processing could be used in biogas production; however,
low efficiency and high cost impede wide application of such
technology. One of the main problems is management and conversion
of organic residues through the anaerobic digestion process which is
characterized by acidic environment due to the low whey pH (
Abstract: Pomegranate (Punica granatum L.) is an ancient fruit of great medical interest and rich source of antioxidants. Pesticides as dimethoate play a crucial role in the occurrence many diseases in plants, animal and human. Therefore the ability of Pomegranate (Punica granatum L.) to alleviate hepatotoxicity induced by organophosphate pesticide dimethoate was investigated. Albino male rats were divided randomly into 4 groups and kept at 7 animals per group in an environmentally controlled condition for 6 weeks. The first group was served as a control group (basal diet), the second group fed on basal diet supplemented with 5% freeze dried pomegranate seeds, the third group fed on 20 ppm dimethoate contaminated diet and the last group fed on dimethoate contaminated diet supplemented with 5% freeze dried pomegranate seeds. The results revealed that administration of dimethoate caused high significant increased in liver functions: alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activities as well as lipid peroxide (malonaldhyde, MDA); on the other hand high significant decreased on glutathione (GSH), glutathione peroxidase (GPx), albumin and total protein were observed. However addition of 5% freeze dried pomegranate seeds significantly improved all previously mentioned parameters. These results indicate the dimethoate induced hepatotoxicity and highlight the protective effect of pomegranate seeds as a potential protective agent against dimethoate induced hepatotoxicity. This may be attributed to the powerful antioxidants (polyphenols, total phenols, and total flavonoids) which present in high levels in pomegranate as well as improving the immunity by activation of antioxidant enzymes GSH and GPx.
Abstract: Natural antimicrobials are used to preserve foods that
can be found in plants, animals, and microorganisms. Antimicrobial
substances are natural or artificial agents that produced by
microorganisms or obtained semi/total chemical synthesis are used at
low concentrations to inhibit the growth of other microorganisms.
Food borne pathogens and spoilage microorganisms are inactivated
by the use of antagonistic microorganisms and their metabolites.
Yeasts can produce toxic proteins or glycoproteins (toxins) that cause
inhibition of sensitive bacteria and yeast species. Antimicrobial
substance producing phenotypes belonging different yeast genus
were isolated from different sources. Toxins secreted by many yeast
strains inhibiting the growth of other yeast strains. These strains show
antimicrobial activity, inhibiting the growth of mold and bacteria.
The effect of antimicrobial agents produced by yeasts can be
extremely fast, and therefore may be used in various treatment
procedures. Rapid inhibition of microorganisms is possibly caused by
microbial cell membrane lipopolysaccharide binding and in
activation (neutralization) effect. Antimicrobial agents inhibit the
target cells via different mechanisms of action.
Abstract: An analysis of the Australian Diabetes Screening
Study estimated undiagnosed diabetes mellitus [DM] prevalence in a
high risk general practice based cohort. DM prevalence varied from
9.4% to 18.1% depending upon the diagnostic criteria utilised with
age being a highly significant risk factor. Utilising the gold standard
oral glucose tolerance test, the prevalence of DM was 22-23% in
those aged >= 70 years and
Abstract: One of the major goals of Spoken Dialog Systems
(SDS) is to understand what the user utters.
In the SDS domain, the Spoken Language Understanding (SLU)
Module classifies user utterances by means of a pre-definite
conceptual knowledge. The SLU module is able to recognize only the
meaning previously included in its knowledge base. Due the vastity
of that knowledge, the information storing is a very expensive
process.
Updating and managing the knowledge base are time-consuming
and error-prone processes because of the rapidly growing number of
entities like proper nouns and domain-specific nouns. This paper
proposes a solution to the problem of Name Entity Recognition
(NER) applied to a SDS domain. The proposed solution attempts to
automatically recognize the meaning associated with an utterance by
using the PANKOW (Pattern based Annotation through Knowledge
On the Web) method at runtime.
The method being proposed extracts information from the Web to
increase the SLU knowledge module and reduces the development
effort. In particular, the Google Search Engine is used to extract
information from the Facebook social network.
Abstract: With the rapid progress of modern cities, the railway
construction must be developing quickly in China.As a typical
high-density country, shopping center on the subway should be one
important factor during the process of urban development. The paper
discusses the influence of the layout of shopping center on the subway,
and put it in the time and space’s axis of Shanghai urban development.
We usethe digital technology to establish the database of relevant
information. And then get the change role about shopping center on
subway in Shanghaiby the Kernel density estimate.The result shows
the development of shopping center on subway has a relationship with
local economic strength, population size, policysupport, and city
construction. And the suburbanization trend of shopping center would
be increasingly significant.By this case research, we could see the
Kernel density estimate is an efficient analysis method on the spatial
layout. It could reveal the characters of layout form of shopping center
on subway in essence. And it can also be applied to the other research
of space form.
Abstract: Recently, to cope with the rapidly changing
construction trend with aging infrastructures, modular bridge
technology has been studied actively. Modular bridge is easily
constructed by assembling standardized precast structure members in
the field. It will be possible to construct rapidly and reduce
construction cost efficiently. However, the shape of the transverse
connection of T-type girder newly developed between the segmented
modules is not verified. Therefore, the verification of the connection
shape is needed. In this study, shape of the modular T-girder bridge
transverse connection was analyzed by finite element model that was
verified in study which was verified model of transverse connection
using Abaqus. Connection angle was chosen as the parameter. The
result of analyses showed that optimal value of angle is 130 degree.
Abstract: In the present study, M2 high speed steels were
fabricated by using electro-slag rapid remelting process. Carbide
structure was analysed and the fracture toughness and hardness were
also measured after austenitization treatment at 1190 and 1210oC
followed by tempering treatment at 535oC for billets with various
diameters from 16 to 60 mm. Electro-slag rapid remelting (ESRR)
process is an advanced ESR process combined by continuous casting
and successfully employed in this study to fabricate a sound M2 high
speed ingot. Three other kinds of commercial M2 high speed steels,
produced by traditional method, were also analysed for comparison.
Distribution and structure of eutectic carbides of the ESRR billet were
found to be comparable to those of commercial alloy and so was the
fracture toughness.
Abstract: The ever increasing amount of solid waste (SW)
generated which is exacerbated by lack of proper waste management
system is of growing concern worldwide and in major cities in
developing countries due to its social, economic and environmental
implications. This study attempts to describe the aspects of solid
waste management (SWM) in Adama, one of the fast urbanizing
cities in Ethiopia, and highlights the challenges thereof. Data were
gathered through interview supplemented by field observation and
self-administered questionnaire. Then, the data were analyzed using
the Statistical Package for Social Science (SPSS) software. In
addition, secondary data were gathered from documents. Findings
revealed that the current SWM practice couldn’t cope with the fast
urbanizing needs and the rapid population growth exhibited by the
city. Besides, major factors contributing to the inefficient system
were identified. The study would provide practical insights to
decision makers in developing a sustainable SWM system leading to
minimized risk in the city.
Abstract: In this study, six bacterial isolates of a slightly
thermophilic organism from the Sg. Klah hot spring, Malaysia were
successfully isolated and designated as M7T55D1, M7T55D2,
M7T55D3, M7T53D1, M7T53D2 and M7T53D3 respectively. The
bacterial isolates were screened for their cellulose hydrolytic ability
on Carboxymethlycellulose agar medium. The isolated bacterial
strains were identified morphologically, biochemically and
molecularly with the aid of 16S rDNA sequencing. All of the bacteria
showed their optimum growth at a slightly alkaline pH of 7.5 with a
temperature of 55°C. All strains were Gram-negative, non-spore
forming type, strictly aerobic, catalase-positive and oxidase-positive
with the ability to produce thermostable cellulase. Based on BLASTn
results, bacterial isolates of M7T55D2 and M7T53D1 gave the
highest homology (97%) with similarity to Tepidimonas ignava while
isolates M7T55D1, M7T55D3, M7T53D2 and M7T53D3 showed
their closest homology (97%-98%) with Tepidimonas thermarum.
These cellulolytic thermophiles might have a commercial potential to
produce valuable thermostable cellulase.