Immobilization of Aspergillus awamori 1-8 for Subsequent Pectinase Production

The overall objective of this research is a strain improvement technology for efficient pectinase production. A novel cells cultivation technology by immobilization of fungal cells has been studied in long time continuous fermentations. Immobilization was achieved by using of new material for absorption of stores of immobilized cultures which was for the first time used for immobilization of microorganisms. Effects of various conditions of nitrogen and carbon nutrition on the biosynthesis of pectolytic enzymes in Aspergillus awamori 1-8 strain were studied. Proposed cultivation technology along with optimization of media components for pectinase overproduction led to increased pectinase productivity in Aspergillus awamori 1-8 from 7 to 8 times. Proposed technology can be applied successfully for production of major industrial enzymes such as α-amylase, protease, collagenase etc.

Multidimensional Visualization Tools for Analysis of Expression Data

Expression data analysis is based mostly on the statistical approaches that are indispensable for the study of biological systems. Large amounts of multidimensional data resulting from the high-throughput technologies are not completely served by biostatistical techniques and are usually complemented with visual, knowledge discovery and other computational tools. In many cases, in biological systems we only speculate on the processes that are causing the changes, and it is the visual explorative analysis of data during which a hypothesis is formed. We would like to show the usability of multidimensional visualization tools and promote their use in life sciences. We survey and show some of the multidimensional visualization tools in the process of data exploration, such as parallel coordinates and radviz and we extend them by combining them with the self-organizing map algorithm. We use a time course data set of transitional cell carcinoma of the bladder in our examples. Analysis of data with these tools has the potential to uncover additional relationships and non-trivial structures.

Relational Impact of Job Stress on Gender Based Managerial Effectiveness in Ghanaian Organizations

This study explored the relationship between occupational stress and the perceived effectiveness of men and women managers in Ghanaian organizations. The exploration is underlined by attempt to understand the degree to which male and female managers in Ghanaian organizations experience occupational stress at the workplace. The purpose is to examine the sources and extents of occupational stress experienced by male and female managers in Ghana. Data was collected using questionnaires and analyzed using both descriptive statistics and correlation analysis. The results showed that female managers in Ghana are more likely to report of more stress experiences in the workplace than their male counterparts. The female managers are more likely to perceive role conflict and alienation as job stressors while the male managers perceived blocked career as a major source of workplace stress. It is concluded that despite the female managers experiencing enormous level of occupational stress, there was no significant differences between their managerial effectiveness and that of the male.

Enhanced Coagulation of Disinfection By-Products Precursors in Porsuk Water Resource, Eskisehir

Natural organic matter (NOM) is heterogeneous mixture of organic compounds that enter the water media from animal and plant remains, domestic and industrial wastes. Researches showed that NOM is likely precursor material for disinfection by products (DBPs). Chlorine very commenly used for disinfection purposes and NOM and chlorine reacts then Trihalomethane (THM) and Haloacetic acids (HAAs) which are cancerogenics for human health are produced. The aim of the study is to search NOM removal by enhanced coagulation from drinking water source of Eskisehir which is supplied from Porsuk Dam. Recently, Porsuk dam water is getting highly polluted and therefore NOM concentration is increasing. Enhanced coagulation studies were evaluated by measurement of Dissolved Organic Carbon (DOC), UV absorbance at 254 nm (UV254), and different trihalomethane formation potential (THMFP) tests. Results of jar test experiments showed that NOM can be removed from water about 40-50 % of efficiency by enhanced coagulation. Optimum coagulant type and coagulant dosages were determined using FeCl3 and Alum.

Information System Life Cycle: Applications in Construction and Manufacturing

In this paper, we present the information life cycle, and analyze the importance of managing the corporate application portfolio across this life cycle. The approach presented here does not correspond just to the extension of the traditional information system development life cycle. This approach is based in the generic life cycle employed in other contexts like manufacturing or marketing. In this paper it is proposed a model of an information system life cycle, supported in the assumption that a system has a limited life. But, this limited life may be extended. This model is also applied in several cases; being reported here two examples of the framework application in a construction enterprise, and in a manufacturing enterprise.

Authentication and Data Hiding Using a Reversible ROI-based Watermarking Scheme for DICOM Images

In recent years image watermarking has become an important research area in data security, confidentiality and image integrity. Many watermarking techniques were proposed for medical images. However, medical images, unlike most of images, require extreme care when embedding additional data within them because the additional information must not affect the image quality and readability. Also the medical records, electronic or not, are linked to the medical secrecy, for that reason, the records must be confidential. To fulfill those requirements, this paper presents a lossless watermarking scheme for DICOM images. The proposed a fragile scheme combines two reversible techniques based on difference expansion for patient's data hiding and protecting the region of interest (ROI) with tamper detection and recovery capability. Patient's data are embedded into ROI, while recovery data are embedded into region of non-interest (RONI). The experimental results show that the original image can be exactly extracted from the watermarked one in case of no tampering. In case of tampered ROI, tampered area can be localized and recovered with a high quality version of the original area.

The effect of the Thickness of Electrical sheet on Overvoltage in the Asynchronous Motors Fed by PWM- converters

This work is devoted to the calculation of the undulatory parameters and the study of the influence thickness of electrical sheet on overvoltage compared to the carcass and between whorls (sections) of the asynchronous motors supplied with PWM converters.

Study of Peptide Fragment of Alpha-Fetoprotein as a Radionuclide Vehicle

Alpfa-fetoprotein and its fragments may be an important vehicle for targeted delivery of radionuclides to the tumor. We investigated the effect of conditions on the labeling of biologically active synthetic peptide based on the (F-afp) with technetium-99m. The influence of the nature of the buffer solution, pH, concentration of reductant, concentration of the peptide and the reaction temperature on the yield of labeling was examined. As a result, the following optimal conditions for labeling of (F-afp) are found: pH 8.5 (phosphate and bicarbonate buffers) and pH from 1.7 to 7.0 (citrate buffer). The reaction proceeds with sufficient yield at room temperature for 30 min at the concentration of SnCl2 and (Fafp) (F-afp) is to be less than 10 mkg/ml and 25 mkg/ml, respectively. Investigations of the test drug accumulation in the tumor cells of human breast cancer were carried out. Results can be assumed that the in vivo study of the (F-afp) in experimental tumor lesions will show concentrations sufficient for imaging these lesions by SPECT.

Noninvasive, Wireless Textronic System to Breath Frequency Measurement

In this paper authors presented the research of textile electroconductive materials, which can be used to construction sensory textronic shirt to breath frequency measurement. The full paper also will present results of measurements carried out on unique measurement stands.

Technological Forecasting on Phytotherapics Development in Brazil

The prospective analysis is presented as an important tool to identify the most relevant opportunities and needs in research and development from planned interventions in innovation systems. This study chose Phyllanthus niruri, known as "stone break" to describe the knowledge about the specie, by using biotechnological forecasting through the software Vantage Point. It can be seen a considerable increase in studies on Phyllanthus niruri in recent years and that there are patents about this plant since twenty-five years ago. India was the country that most carried out research on the specie, showing interest, mainly in studies of hepatoprotection, antioxidant and anti-cancer activities. Brazil is in the second place, with special interest for anti-tumor studies. Given the identification of the Brazilian groups that exploit the species it is possible to mediate partnerships and cooperation aiming to help on the implementing of the Program of Herbal medicines (phytotherapics) in Brazil.

Ab Initio Molecular Dynamics Simulations of Furfural at the Liquid-Solid Interface

The bonding configuration and the heat of adsorption of a furfural molecule on the Pd(111) surface were determined by ab initio density-functional-theory calculations. The dynamics of pure liquid water, the liquid-solid interface formed by liquid water and the Pd(111) surface, as well as furfural at the water-Pd interface, were investigated by ab initio molecular dynamics simulations at finite temperatures. Calculations and simulations suggest that the bonding configurations at the water-Pd interface promote decarbonylation of furfural.

Adsorption of Crystal Violet onto BTEA- and CTMA-bentonite from Aqueous Solutions

CTMA-bentonite and BTEA-Bentonite prepared by Na-bentonite cation exchanged with cetyltrimethylammonium(CTMA) and benzyltriethylammonium (BTEA). Products were characterized by XRD and IR techniques.The d001 spacing value of CTMA-bentonite and BTEA-bentonite are 7.54Å and 3.50Å larger than that of Na-bentonite at 100% cation exchange capacity, respectively. The IR spectrum showed that the intensities of OH stretching and bending vibrations of the two organoclays decreased greatly comparing to untreated Na-bentonite. Batch experiments were carried out at 303 K, 318 K and 333 K to obtain the sorption isotherms of Crystal violet onto the two organoclays. The results show that the sorption isothermal data could be well described by Freundlich model. The dynamical data for the two organoclays fit well with pseudo-second-order kinetic model. The adsorption capacity of CTMA-bentonite was found higher than that of BTEA-Bentonite. Thermodynamic parameters such as changes in the free energy (ΔG°), the enthalpy (ΔH°) and the entropy (ΔS°) were also evaluated. The overall adsorption process of Crystal violet onto the two organoclays were spontaneous, endothermic physisorption. The CTMA-bentonite and BTEA-Bentonite could be employed as low-cost alternatives to activated carbon in wastewater treatment for the removal of color which comes from textile dyes.

Surface Roughness Optimization in End Milling Operation with Damper Inserted End Milling Cutters

This paper presents a study of the Taguchi design application to optimize surface quality in damper inserted end milling operation. Maintaining good surface quality usually involves additional manufacturing cost or loss of productivity. The Taguchi design is an efficient and effective experimental method in which a response variable can be optimized, given various factors, using fewer resources than a factorial design. This Study included spindle speed, feed rate, and depth of cut as control factors, usage of different tools in the same specification, which introduced tool condition and dimensional variability. An orthogonal array of L9(3^4)was used; ANOVA analyses were carried out to identify the significant factors affecting surface roughness, and the optimal cutting combination was determined by seeking the best surface roughness (response) and signal-to-noise ratio. Finally, confirmation tests verified that the Taguchi design was successful in optimizing milling parameters for surface roughness.

Comparison of Anti-Shadoo Antibodies – Where is the Endogenous Shadoo protein?

Shadoo protein (Sho) was described in 2003 as the newest member of Prion protein superfamily [1]. Sho has similar structural motifs like prion protein (PrP) that is known for its central role in transmissible spongiform enchephalopathies. Although a great number of functions have been proposed, the exact physiological function of PrP is not known yet. Investigation of the function and localization of Sho may help us to understand the function of the Prion protein superfamily. Analyzing the subcellular localization of YFP-tagged forms of Sho, we detected the protein in the plasma membrane and in the nucleus of various cell lines. To reveal the localization of the endogenous protein we generated antibodies against Shadoo as well as employed commercially available anti-Shadoo antibodies: i) EG62 anti-mouse Shadoo antibody generated by Eurogentec Ltd.; ii) S-12 anti-human Shadoo antibody by Santa Cruz Biotechnology Inc.; iii) R-12 anti-mouse Shadoo antibody by Santa Cruz Biotechnology Inc.; iv) SPRN antibody against human Shadoo by Abgent Inc. We carried out immunocytochemistry on non-transfected HeLa, Zpl 2-1, Zw 3-5, GT1-1, GT1-7 and SHSY5Y cells as well as on YFP-Sho, Sho-YFP, and YFP-GPI transfected HeLa cells. Their specificity (in antibody-peptide competition assay) and co-localization (with the YFP signal) were assessed.

Finite Element Analysis of Thin Steel Plate Shear Walls

Steel plate shear walls (SPSWs) in buildings are known to be an effective means for resisting lateral forces. By using un-stiffened walls and allowing them to buckle, their energy absorption capacity will increase significantly due to the postbuckling capacity. The post-buckling tension field action of SPSWs can provide substantial strength, stiffness and ductility. This paper presents the Finite Element Analysis of low yield point (LYP) steel shear walls. In this shear wall system, the LYP steel plate is used for the steel panel and conventional structural steel is used for boundary frames. A series of nonlinear cyclic analyses were carried out to obtain the stiffness, strength, deformation capacity, and energy dissipation capacity of the LYP steel shear wall. The effect of widthto- thickness ratio of steel plate on buckling behavior, and energy dissipation capacities were studied. Good energy dissipation and deformation capacities were obtained for all models.

Electronic Government in the GCC Countries

The study investigated the practices of organisations in Gulf Cooperation Council (GCC) countries with regards to G2C egovernment maturity. It reveals that e-government G2C initiatives in the surveyed countries in particular, and arguably around the world in general, are progressing slowly because of the lack of a trusted and secure medium to authenticate the identities of online users. The authors conclude that national ID schemes will play a major role in helping governments reap the benefits of e-government if the three advanced technologies of smart card, biometrics and public key infrastructure (PKI) are utilised to provide a reliable and trusted authentication medium for e-government services.

A Method to Predict Hemorrhage Disease of Grass Carp Tends

Hemorrhage Disease of Grass Carp (HDGC) is a kind of commonly occurring illnesses in summer, and the extremely high death rate result in colossal losses to aquaculture. As the complex connections among each factor which influences aquiculture diseases, there-s no quit reasonable mathematical model to solve the problem at present.A BP neural network which with excellent nonlinear mapping coherence was adopted to establish mathematical model; Environmental factor, which can easily detected, such as breeding density, water temperature, pH and light intensity was set as the main analyzing object. 25 groups of experimental data were used for training and test, and the accuracy of using the model to predict the trend of HDGC was above 80%. It is demonstrated that BP neural network for predicating diseases in HDGC has a particularly objectivity and practicality, thus it can be spread to other aquiculture disease.

3D Numerical Simulation of Scouring around Bridge Piers (Case Study: Bridge 524 Crosses the Tanana River)

Due to the three- dimensional flow pattern interacting with bed material, the process of local scour around bridge piers is complex. Modeling 3D flow field and scour hole evolution around a bridge pier is more feasible nowadays because the computational cost and computational time have significantly decreased. In order to evaluate local flow and scouring around a bridge pier, a completely three-dimensional numerical model, SSIIM program, was used. The model solves 3-D Navier-Stokes equations and a bed load conservation equation. The model was applied to simulate local flow and scouring around a bridge pier in a large natural river with four piers. Computation for 1 day of flood condition was carried out to predict the maximum local scour depth. The results show that the SSIIM program can be used efficiently for simulating the scouring in natural rivers. The results also showed that among the various turbulence models, the k-ω model gives more reasonable results.

Mycorrhizal Fungi Influence on Physiological Growth Indices in Basil Induced by Phosphorus Fertilizer under Irrigation Deficit Conditions

This experiment was carried out to study the effect of AMF, drought stress and phosphorus on physiological growth indices of basil at Iran using by a split-plot design with three replications. The main-plot factor included: two levels of irrigation regimes (control=no drought stress and irrigation after 80 evaporation= drought stress condition) while the sub-plot factors included phosphorus (0, 35 and 70 kg/ha) and application and non-application of Glomus fasciculatum. The results showed that total dry matter (TDM), life area index (LAI), relative growth rate (RGR) and crop growth rate (CGR) were all highly significantly different among the phosphorus, whereas drought stress had effect of practical significance on TDM, LAI, RGR and CGR. The results also showed that the highest TDM, LAI, RGR and CGR were obtained from application of Glomus fasciculatum under no-drought condition.

All Proteins Have a Basic Molecular Formula

This study proposes a basic molecular formula for all proteins. A total of 10,739 proteins belonging to 9 different protein groups classified on the basis of their functions were selected randomly. They included enzymes, storage proteins, hormones, signalling proteins, structural proteins, transport proteins, immunoglobulins or antibodies, motor proteins and receptor proteins. After obtaining the protein molecular formula using the ProtParam tool, the H/C, N/C, O/C, and S/C ratios were determined for each randomly selected sample. In this case, H, N, O, and S coefficients were specified per carbon atom. Surprisingly, the results demonstrated that H, N, O, and S coefficients for all 10,739 proteins are similar and highly correlated. This study demonstrates that despite differences in the structure and function, all known proteins have a similar basic molecular formula CnH1.58 ± 0.015nN0.28 ± 0.005nO0.30 ± 0.007nS0.01 ± 0.002n. The total correlation between all coefficients was found to be 0.9999.