Abstract: Soil microbial activity is adversely affected by pollutants such as heavy metals, antibiotics and pesticides. Organic amendments including sewage sludge, municipal compost and vermicompost are recently used to improve soil structure and fertility. But, these materials contain heavy metals including Pb, Cd, Zn, Ni and Cu that are toxic to soil microorganisms and may lead to occurrence of more tolerant microbes. Among these, Pb is the most abundant and has more negative effect on soil microbial ecology. In this study, Pb levels of 0, 100, 200, 300, 400 and 500 mg Pb [as Pb(NO3)2] per kg soil were added to the pots containing 2 kg of a loamy soil and incubated for 6 months at 25°C with soil moisture of - 0.3 MPa. Dehydrogenase activity of soil as a measure of microbial activity was determined on 15, 30, 90 and 180 days after incubation. Triphenyl tetrazolium chloride (TTC) was used as an electron acceptor in this assay. PICTs (IC50 values) were calculated for each Pb level and incubation time. Soil microbial activity was decreased by increasing Pb level during 30 days of incubation but the induced tolerance appeared on day 90 and thereafter. During 90 to 180 days of incubation, the PICT was gradually developed by increasing Pb level up to 200 mg kg-1, but the rate of enhancement was steeper at higher concentrations.
Abstract: Low temperature (LT) is one of the most abiotic
stresses causing loss of yield in wheat (T. aestivum). Four major
genes in wheat (Triticum aestivum L.) with the dominant alleles
designated Vrn–A1,Vrn–B1,Vrn–D1 and Vrn4, are known to have
large effects on the vernalization response, but the effects on cold
hardiness are ambiguous. Poor cold tolerance has restricted winter
wheat production in regions of high winter stress [9]. It was known
that nearly all wheat chromosomes [5] or at least 10 chromosomes of
21 chromosome pairs are important in winter hardiness [15]. The
objective of present study was to clarify the role of each chromosome
in cold tolerance. With this purpose we used 20 isogenic lines of
wheat. In each one of these isogenic lines only a chromosome from
‘Bezostaya’ variety (a winter habit cultivar) was substituted to
‘Capple desprez’ variety. The plant materials were planted in
controlled conditions with 20º C and 16 h day length in moderately
cold areas of Iran at Karaj Agricultural Research Station in 2006-07
and the acclimation period was completed for about 4 weeks in a
cold room with 4º C. The cold hardiness of these isogenic lines was
measured by LT50 (the temperature in which 50% of the plants are
killed by freezing stress).The experimental design was completely
randomized block design (RCBD)with three replicates. The results
showed that chromosome 5A had a major effect on freezing
tolerance, and then chromosomes 1A and 4A had less effect on this
trait. Further studies are essential to understanding the importance of
each chromosome in controlling cold hardiness in wheat.
Abstract: In the present Jordan hotels scenario, service quality is
a vital competitive policy to keep customer support and build great
base. Hotels are trying to win customer loyalty by providing enhanced
quality services. This paper attempts to examine the impact of tourism
service quality dimension in the Jordanian five star hotels. A total of
322 surveys were administrated to tourists who were staying at three
branches Marriott hotel in Jordan. The results show that dimensions of
service quality such as empathy, reliability, responsiveness and
tangibility significantly predict customer loyalty. Specifically, among
the dimension of tourism service quality, the most significant predictor
of customer loyalty is tangibility. This paper implies that five star
hotels in Jordan should also come forward and try their best to present
better tourism service quality to win back their customers- loyalty.
Abstract: The nexus between language and culture is so
intertwined and very significant that language is largely seen as a
vehicle for cultural transmission. Culture itself refers to the aggregate
belief system of a people, embellishing its corporate national image
or brand. If we conceive national rebranding as a campaign to
rekindle the patriotic flame in the consciousness of a people towards
its sociocultural imperatives and values, then, Nigerian indigenous
linguistic flame has not been ignited. Consequently, the paper
contends that the current national rebranding policy remains a myth
in the confines of the elitists' intellectual squabble. It however
recommends that the use of our indigenous languages should be
supported by adequate legislation and also propagated by Nollywood
in order to revamp and sustain the people’s interest in their local
languages. Finally, the use of the indigenous Nigerian languages
demonstrates patriotism, an important ingredient for actualizing a
genuine national rebranding.
Abstract: Taking into account the importance of Lake Sevan and
Lake Sevan basin-s rivers for Armenian economy, the main goals of
our investigations were the documentation of water quality and the
biodiversity of invertebrates developed in Lake Sevan basin-s rivers
and selected tributaries.
Moderately satisfied ecological condition for the biodiversity of
Lake Sevan basin-s rivers has been established, and the changes in
species- composition of zoobenthos in Lake Sevan were detected.
A growing tendency of antibiotic resistance among E. coli isolates
in water resources has been shown.
Abstract: The National Agricultural Biotechnology Information
Center (NABIC) plays a leading role in the biotechnology information
database for agricultural plants in Korea. Since 2002, we have
concentrated on functional genomics of major crops, building an
integrated biotechnology database for agro-biotech information that
focuses on bioinformatics of major agricultural resources such as rice,
Chinese cabbage, and microorganisms. In the NABIC,
integration-based biotechnology database provides useful information
through a user-friendly web interface that allows analysis of genome
infrastructure, multiple plants, microbial resources, and living
modified organisms.
Abstract: The identification and classification of the spine deformity play an important role when considering surgical planning for adolescent patients with idiopathic scoliosis. The subject of this article is the Lenke classification of scoliotic spines using Cobb angle measurements. The purpose is two-fold: (1) design a rulebased diagram to assist clinicians in the classification process and (2) investigate a computer classifier which improves the classification time and accuracy. The rule-based diagram efficiency was evaluated in a series of scoliotic classifications by 10 clinicians. The computer classifier was tested on a radiographic measurement database of 603 patients. Classification accuracy was 93% using the rule-based diagram and 99% for the computer classifier. Both the computer classifier and the rule based diagram can efficiently assist clinicians in their Lenke classification of spine scoliosis.
Abstract: The increasing usage of antibiotics in the animal
farming industry is an emerging worldwide problem contributing to
the development of antibiotic resistance. The purpose of this work was
to investigate the prevalence and antibiotic resistance profile of
bacterial isolates collected from aquatic environments and meats in a
peri-urban community in Daejeon, Korea. In an antibacterial
susceptibility test, the bacterial isolates showed a high incidence of
resistance (~ 26.04 %) to cefazolin, tetracycline, gentamycin,
norfloxacin, erythromycin and vancomycin. The results from a test for
multiple antibiotic resistance indicated that the isolates were
displaying an approximately 5-fold increase in the incidence of
multiple antibiotic resistance to combinations of two different
antibiotics compared to combinations of three or more antibiotics.
Most of the isolates showed multi-antibiotic resistance, and the
resistance patterns were similar among the sampling groups.
Sequencing data analysis of 16S rRNA showed that most of the
resistant isolates appeared to be dominated by the classes
Betaproteobacteria and Gammaproteobacteria in the phylum
Proteobacteria.
Abstract: According to FDA (Food and Drug Administration of the United States), vinegar is definedas a sour liquid containing at least 4 grams acetic acid in 100 cubic centimeter (4% solution of acetic acid) of solution that is produced from sugary materials by alcoholic fermentation. In the base of microbial starters, vinegars could be contained of more than 50 types of volatile and aromatic substances that responsible for their sweet taste and smelling. Recently the vinegar industry has a great proportion in agriculture, food and microbial biotechnology. The acetic acid bacteria are from the family Acetobacteraceae. Regarding to the latest version of Bergy-s Mannual of Systematic Bacteriology that has categorized bacteria in the base of their 16s RNA differences, the most important acetic acid genera are included Acetobacter (genus I), Gluconacetobacter (genus VIII) and Gluconobacter (genus IX). The genus Acetobacter that is primarily used in vinegar manufacturing plants is a gram negative, obligate aerobe coccus or rod shaped bacterium with the size 0.6 - 0.8 X 1.0 - 4.0 μm, nonmotile or motile with peritrichous flagella and catalase positive – oxidase negative biochemically. Some strains are overoxidizer that could convert acetic acid to carbon dioxide and water.In this research one Acetobacter native strain with high acetic acid productivity was isolated from Iranian white – red cherry. We used two specific culture media include Carr medium [yeast extract, 3%; ethanol, 2% (v/v); bromocresol green, 0.002%; agar, 2% and distilled water, 1000 ml], Frateur medium [yeast extract, 10 g/l; CaCO3, 20 g/l; ethanol, 20 g/l; agar, 20 g/l and distilled water, 1000 ml] and an industrial culture medium. In addition to high acetic acid production and high growth rate, this strain had a good tolerance against ethanol concentration that was examined using modified Carr media with 5%, 7% and 9% ethanol concentrations. While the industrial strains of acetic acid bacteria grow in the thermal range of 28 – 30 °C, this strain was adapted for growth in 34 – 36 °C after 96 hours incubation period. These dramatic characteristics suggest a potential biotechnological strain in production of cherry vinegar with a sweet smell and different nutritional properties in comparison to recent vinegar types. The lack of growth after 24, 48 and 72 hours incubation at 34 – 36 °C and the growth after 96 hours indicates a good and fast thermal flexibility of this strain as a significant characteristic of biotechnological and industrial strains.
Abstract: Thyroid cancer-s overall contribution to the
worldwide cancer burden is relatively small, but incidence rates have increased over the last three decades throughout the world. This trend has been hypothesised to reflect a combination of technological advances enabling increased detection, but also changes in
environmental factors, including population exposure to ionising radiation from fallout, diagnostic tests and treatment for benign and
malignant conditions. The Thyroid dose received apparently shielded
by cerrobend blocks was about 8cGy in 100cGy Expose
Abstract: The mechanism of abiotic stress tolerance is crucial
for plants to survive in harsh condition and the knowledge of this
mechanism can be use to solve the problem of declining productivity
of plants or crops around the world. However in-depth description is
still unclear and it is argued, in particular that there is a relationship
between high salinity tolerance and the ability to tolerate high light
condition. In this study, Dunaliella salina, which can withstand high
salt was used as a model. Chlorophyll fluorometer for nonphotochemical
quenching (NPQ) measurement and high-performance
liquid chromatography for pigment determination was used. The
results show that NPQ value and the amount of pigment were
increased along with the levels of salinity. However, it establish a
clear relationship between high salt and high light but the further
study to optimized the solutions mentioned above is still required.
Abstract: Surface water pollution is one of the serious
environmental problems in rural areas of South Africa due to
discharge of household waste into the streams, turning them into
open sewers. In this study, samples of water were collected from a
stream in Soshanguve and analysed. The result showed that pollution
in the area was caused by man and its activities. The water quality in
the area was found to have deterioted significantly after water runoff
from farms and household wastes. The result shows, fertilizer runoff
contributes 50% of the pollution while pesticides and sediments
contribute up to 10% respectively in the streams, while household
waste contributes up to 30%. This study gives an outline of the
sources of water pollution in the area and provides a process of
creating a clean and unpolluted environment for Soshanguve
community in Pretoria north in order to achieve the 7th aim of the
millennium development goals by 2015, which is ensuring
environmental sustainability.
Abstract: 2D/3D registration is a special case of medical image
registration which is of particular interest to surgeons. Applications
of 2D/3D registration are [1] radiotherapy planning and treatment
verification, spinal surgery, hip replacement, neurointerventions and
aortic stenting. The purpose of this paper is to provide a literature
review of the main methods for image registration for the 2D/3D
case. At the end of the paper an algorithm is proposed for 2D/3D
registration based on the Chebyssev polynomials iteration loop.
Abstract: Mycophenolic acid “MPA" is a secondary metabolite
of Penicillium bervicompactum with antibiotic and
immunosuppressive properties. In this study, fermentation process
was established for production of mycophenolic acid by Penicillium
bervicompactum MUCL 19011 in shake flask. The maximum MPA
production, product yield and productivity were 1.379 g/L, 18.6 mg/g
glucose and 4.9 mg/L.h respectively. Glucose consumption, biomass
and MPA production profiles were investigated during fermentation
time. It was found that MPA production starts approximately after
180 hours and reaches to a maximum at 280 h. In the next step, the
effects of methionine and acetate concentrations on MPA production
were evaluated. Maximum MPA production, product yield and
productivity (1.763 g/L, 23.8 mg/g glucose and 6.30 mg/L. h
respectively) were obtained with using 2.5 g/L methionine in culture
medium. Further addition of methionine had not more positive effect
on MPA production. Finally, results showed that the addition of
acetate to the culture medium had not any observable effect on MPA
production.
Abstract: Food borne illnesses have been reported to be a global
health challenge. Annual incidences of food–related diseases involve
76 million cases, of which only 14 million can be traced to known
pathogens. Poor hygienic practices have contributed greatly to this. It
has been reported that in the year 2000 about 2.1 million people died
from diarrheal diseases, hence, there is a need to ensure food safety at
all level. This study focused on the sterility examination and
inhibitory effect of honey samples on selected gram negative and
gram positive food borne pathogen from South West Nigeria. The
laboratory examinations revealed the presence of some bacterial and
fungal contaminations of honey samples and that inhibitory activity
of the honey sample was more pronounced on the gram negative
bacteria than the gram positive bacterial isolates. Antibiotic
sensitivity test conducted on the different bacterial isolates also
showed that honey was able to inhibit the proliferation of the tested
bacteria than the employed antibiotics.
Abstract: The major building block of most elliptic curve cryptosystems
are computation of multi-scalar multiplication. This paper
proposes a novel algorithm for simultaneous multi-scalar multiplication,
that is by employing addition chains. The previously known
methods utilizes double-and-add algorithm with binary representations.
In order to accomplish our purpose, an efficient empirical
method for finding addition chains for multi-exponents has been
proposed.
Abstract: Antimicrobial resistant is becoming a major factor in
virtually all hospital acquired infection may soon untreatable is a
serious public health problem. These concerns have led to major
research effort to discover alternative strategies for the treatment of
bacterial infection. Nanobiotehnology is an upcoming and fast
developing field with potential application for human welfare. An
important area of nanotechnology for development of reliable and
environmental friendly process for synthesis of nanoscale particles
through biological systems In the present studies are reported on the
use of fungal strain Aspergillus species for the extracellular synthesis
of bionanoparticles from 1 mM silver nitrate (AgNO3) solution. The
report would be focused on the synthesis of metallic bionanoparticles
of silver using a reduction of aqueous Ag+ ion with the
culture supernatants of Microorganisms. The bio-reduction of the
Ag+ ions in the solution would be monitored in the aqueous
component and the spectrum of the solution would measure through
UV-visible spectrophotometer The bionanoscale particles were
further characterized by Atomic Force Microscopy (AFM), Fourier
Transform Infrared Spectroscopy (FTIR) and Thin layer
chromatography. The synthesized bionanoscale particle showed a
maximum absorption at 385 nm in the visible region. Atomic Force
Microscopy investigation of silver bionanoparticles identified that
they ranged in the size of 250 nm - 680 nm; the work analyzed the
antimicrobial efficacy of the silver bionanoparticles against various
multi drug resistant clinical isolates. The present Study would be
emphasizing on the applicability to synthesize the metallic
nanostructures and to understand the biochemical and molecular
mechanism of nanoparticles formation by the cell filtrate in order to
achieve better control over size and polydispersity of the
nanoparticles. This would help to develop nanomedicine against
various multi drug resistant human pathogens.
Abstract: We present a new method for the fully automatic 3D
reconstruction of the coronary artery centerlines, using two X-ray
angiogram projection images from a single rotating monoplane
acquisition system. During the first stage, the input images are
smoothed using curve evolution techniques. Next, a simple yet
efficient multiscale method, based on the information of the Hessian
matrix, for the enhancement of the vascular structure is introduced.
Hysteresis thresholding using different image quantiles, is used to
threshold the arteries. This stage is followed by a thinning procedure
to extract the centerlines. The resulting skeleton image is then pruned
using morphological and pattern recognition techniques to remove
non-vessel like structures. Finally, edge-based stereo correspondence
is solved using a parallel evolutionary optimization method based on
f symbiosis. The detected 2D centerlines combined with disparity
map information allow the reconstruction of the 3D vessel
centerlines. The proposed method has been evaluated on patient data
sets for evaluation purposes.
Abstract: To evaluate genetic variation of wheat (Triticum
aestivum) affected by heat and drought stress on eight Australian
wheat genotypes that are parents of Doubled Haploid (HD) mapping
populations at the vegetative stage, the water stress experiment was
conducted at 65% field capacity in growth room. Heat stress
experiment was conducted in the research field under irrigation over
summer. Result show that water stress decreased dry shoot weight
and RWC but increased osmolarity and means of Fv/Fm values in all
varieties except for Krichauff. Krichauff and Kukri had the
maximum RWC under drought stress. Trident variety was shown
maximum WUE, osmolarity (610 mM/Kg), dry mater, quantum yield
and Fv/Fm 0.815 under water stress condition. However, the
recovery of quantum yield was apparent between 4 to 7 days after
stress in all varieties. Nevertheless, increase in water stress after that
lead to strong decrease in quantum yield. There was a genetic
variation for leaf pigments content among varieties under heat stress.
Heat stress decreased significantly the total chlorophyll content that
measured by SPAD. Krichauff had maximum value of Anthocyanin
content (2.978 A/g FW), chlorophyll a+b (2.001 mg/g FW) and
chlorophyll a (1.502 mg/g FW). Maximum value of chlorophyll b
(0.515 mg/g FW) and Carotenoids (0.234 mg/g FW) content
belonged to Kukri. The quantum yield of all varieties decreased
significantly, when the weather temperature increased from 28 ÔùªC to
36 ÔùªC during the 6 days. However, the recovery of quantum yield
was apparent after 8th day in all varieties. The maximum decrease
and recovery in quantum yield was observed in Krichauff. Drought
and heat tolerant and moderately tolerant wheat genotypes were
included Trident, Krichauff, Kukri and RAC875. Molineux, Berkut
and Excalibur were clustered into most sensitive and moderately
sensitive genotypes. Finally, the results show that there was a
significantly genetic variation among the eight varieties that were
studied under heat and water stress.
Abstract: A full six degrees of freedom (6-DOF) flight dynamics
model is proposed for the accurate prediction of short and long-range
trajectories of high spin and fin-stabilized projectiles via atmospheric
flight to final impact point. The projectiles is assumed to be both rigid
(non-flexible), and rotationally symmetric about its spin axis launched
at low and high pitch angles. The mathematical model is based on the
full equations of motion set up in the no-roll body reference frame and
is integrated numerically from given initial conditions at the firing
site. The projectiles maneuvering motion depends on the most
significant force and moment variations, in addition to wind and
gravity. The computational flight analysis takes into consideration the
Mach number and total angle of attack effects by means of the
variable aerodynamic coefficients. For the purposes of the present
work, linear interpolation has been applied from the tabulated database
of McCoy-s book. The developed computational method gives
satisfactory agreement with published data of verified experiments and
computational codes on atmospheric projectile trajectory analysis for
various initial firing flight conditions.