Cold Hardiness in Near Isogenic Lines of Bread Wheat (Triticum Aestivum L. em. Thell.)
Low temperature (LT) is one of the most abiotic
stresses causing loss of yield in wheat (T. aestivum). Four major
genes in wheat (Triticum aestivum L.) with the dominant alleles
designated Vrn–A1,Vrn–B1,Vrn–D1 and Vrn4, are known to have
large effects on the vernalization response, but the effects on cold
hardiness are ambiguous. Poor cold tolerance has restricted winter
wheat production in regions of high winter stress [9]. It was known
that nearly all wheat chromosomes [5] or at least 10 chromosomes of
21 chromosome pairs are important in winter hardiness [15]. The
objective of present study was to clarify the role of each chromosome
in cold tolerance. With this purpose we used 20 isogenic lines of
wheat. In each one of these isogenic lines only a chromosome from
‘Bezostaya’ variety (a winter habit cultivar) was substituted to
‘Capple desprez’ variety. The plant materials were planted in
controlled conditions with 20º C and 16 h day length in moderately
cold areas of Iran at Karaj Agricultural Research Station in 2006-07
and the acclimation period was completed for about 4 weeks in a
cold room with 4º C. The cold hardiness of these isogenic lines was
measured by LT50 (the temperature in which 50% of the plants are
killed by freezing stress).The experimental design was completely
randomized block design (RCBD)with three replicates. The results
showed that chromosome 5A had a major effect on freezing
tolerance, and then chromosomes 1A and 4A had less effect on this
trait. Further studies are essential to understanding the importance of
each chromosome in controlling cold hardiness in wheat.
[1] Baga M., Chodaparambil S.V., Limin A.E., Pecar M., Fowler D.B.,
Chibbar R.N., (2007). Identification of quantitative trait loci and
assosiated candidate genes for low-temperature tolerance in cold-hardy
winter wheat. Funct. Integr. Genomics. 7: 53-68.
[2] Briggle L.W., and Curtis B.C., (1987). Wheat world wide. In wheat and
wheat improvement. E.G. Heyne(ed). 2nd edition,ASA, CSSA, SSSA,
Madison, WI, USA. pp. 1-32.
[3] Fowler, D.B., Chauvin, L.P., Limin, A.E., and Sarhan, F., 1996a. The
regulatory role of vernalization in the expression of low-temperatureinduced
genes in wheat and rye.Theor. Appl. Genet. 93,554-559.
[4] Fowler, D.B., Limin, A.E., Wang, S.Y., and Ward, R.W., 1996b.
Relationship between low-temperature tolerance and vernalization
response in wheat and rye. Can. J. Plant Sci. 76, 37-42.
[5] Galiba, G., S.A. Quarrie, J. Sutka, A. Morgounov, and J.W. Snape,
(1995). RFLP mapping of the vernalization ( Vrn1 ) and frost resistance (
Fr1 ) genes on chromosome 5A of wheat. Theor. Appl. Genet. 90: 1174-
1179.
[6] Houde, M., R. Dhindsa, and F. Sarhan. (1992). A molecular marker to
select for freezing tolerance in Gramineae. Mol. Gen. Genet. 234: 43-48.
[7] Koemel J.E., Jr., Arron C. Guenzi, Jeffrey A. Anderson. Edward L.
Smith, (2004), Cold hardiness of wheat near-isogenic lines differing in
vernalization alleles. Theor Appl Genet 109: 839-846.
[8] Law, C.N., and G. Jenkins. (1970). A genetic study of cold resistance in
wheat. Genet. Res. 15: 197-208.
[9] Limin, A.E., Fowler, D.B., (1993). Inheritance of Cold Hardiness in
T.aestivum Synthetic hexaploid wheat crosses. Plant Braading. 110: 103-
108
[10] Limin, A.E., Fowler, D.B., (2006).Low-temperature tolerance and
genetic potential in wheat (Triticum aestivum L.): response to
photoperiod, vernalization, and plant development. Planta. 224: 360 -
366.
[11] Limin, A.E., Danyluk J., Chauvin L.P., Fowler, D.B., Sarhan F., (1997).
Chromosome mapping of low-temperature induced Wcs 120 family
genes and regulation of cold-tolerance expression in wheat. Mol. And
Gen. Genet. 253:720-727.
[12] Prasil I., Prasilova P., Papazisis K., and Valter J., (1994). Evaluation of
freezing injury and dynamics of freezing resistance in cereals. In: Crop
Adaptation to Cool Climates. Workshop October 12-14, 1994, Hamburg,
Germany.pp.37-48.
[13] Sutka, J. 1981. Genetic studies of frost resistance in wheat. Theor. Appl.
Genet. 59, 145-152.
[14] Sutka, J., and G. Kovacs. (1985). Reciprocal monosomic analysis of
frost resistance on chromosome 5A in wheat. Euphytica 34: 367-370.
[15] Sutka, J. (2001). Genes for frost resistance in wheat.Z. Bedo and L. lang
(eds), wheat in a global environment kluwer academic publishers.
Printed in the Netherland. 471-479
[1] Baga M., Chodaparambil S.V., Limin A.E., Pecar M., Fowler D.B.,
Chibbar R.N., (2007). Identification of quantitative trait loci and
assosiated candidate genes for low-temperature tolerance in cold-hardy
winter wheat. Funct. Integr. Genomics. 7: 53-68.
[2] Briggle L.W., and Curtis B.C., (1987). Wheat world wide. In wheat and
wheat improvement. E.G. Heyne(ed). 2nd edition,ASA, CSSA, SSSA,
Madison, WI, USA. pp. 1-32.
[3] Fowler, D.B., Chauvin, L.P., Limin, A.E., and Sarhan, F., 1996a. The
regulatory role of vernalization in the expression of low-temperatureinduced
genes in wheat and rye.Theor. Appl. Genet. 93,554-559.
[4] Fowler, D.B., Limin, A.E., Wang, S.Y., and Ward, R.W., 1996b.
Relationship between low-temperature tolerance and vernalization
response in wheat and rye. Can. J. Plant Sci. 76, 37-42.
[5] Galiba, G., S.A. Quarrie, J. Sutka, A. Morgounov, and J.W. Snape,
(1995). RFLP mapping of the vernalization ( Vrn1 ) and frost resistance (
Fr1 ) genes on chromosome 5A of wheat. Theor. Appl. Genet. 90: 1174-
1179.
[6] Houde, M., R. Dhindsa, and F. Sarhan. (1992). A molecular marker to
select for freezing tolerance in Gramineae. Mol. Gen. Genet. 234: 43-48.
[7] Koemel J.E., Jr., Arron C. Guenzi, Jeffrey A. Anderson. Edward L.
Smith, (2004), Cold hardiness of wheat near-isogenic lines differing in
vernalization alleles. Theor Appl Genet 109: 839-846.
[8] Law, C.N., and G. Jenkins. (1970). A genetic study of cold resistance in
wheat. Genet. Res. 15: 197-208.
[9] Limin, A.E., Fowler, D.B., (1993). Inheritance of Cold Hardiness in
T.aestivum Synthetic hexaploid wheat crosses. Plant Braading. 110: 103-
108
[10] Limin, A.E., Fowler, D.B., (2006).Low-temperature tolerance and
genetic potential in wheat (Triticum aestivum L.): response to
photoperiod, vernalization, and plant development. Planta. 224: 360 -
366.
[11] Limin, A.E., Danyluk J., Chauvin L.P., Fowler, D.B., Sarhan F., (1997).
Chromosome mapping of low-temperature induced Wcs 120 family
genes and regulation of cold-tolerance expression in wheat. Mol. And
Gen. Genet. 253:720-727.
[12] Prasil I., Prasilova P., Papazisis K., and Valter J., (1994). Evaluation of
freezing injury and dynamics of freezing resistance in cereals. In: Crop
Adaptation to Cool Climates. Workshop October 12-14, 1994, Hamburg,
Germany.pp.37-48.
[13] Sutka, J. 1981. Genetic studies of frost resistance in wheat. Theor. Appl.
Genet. 59, 145-152.
[14] Sutka, J., and G. Kovacs. (1985). Reciprocal monosomic analysis of
frost resistance on chromosome 5A in wheat. Euphytica 34: 367-370.
[15] Sutka, J. (2001). Genes for frost resistance in wheat.Z. Bedo and L. lang
(eds), wheat in a global environment kluwer academic publishers.
Printed in the Netherland. 471-479
@article{"International Journal of Biological, Life and Agricultural Sciences:53446", author = "Abolfazl Rashidi Asl and Siroos Mahfoozi and Mohammad Reza Bihamta", title = "Cold Hardiness in Near Isogenic Lines of Bread Wheat (Triticum Aestivum L. em. Thell.)", abstract = "Low temperature (LT) is one of the most abiotic
stresses causing loss of yield in wheat (T. aestivum). Four major
genes in wheat (Triticum aestivum L.) with the dominant alleles
designated Vrn–A1,Vrn–B1,Vrn–D1 and Vrn4, are known to have
large effects on the vernalization response, but the effects on cold
hardiness are ambiguous. Poor cold tolerance has restricted winter
wheat production in regions of high winter stress [9]. It was known
that nearly all wheat chromosomes [5] or at least 10 chromosomes of
21 chromosome pairs are important in winter hardiness [15]. The
objective of present study was to clarify the role of each chromosome
in cold tolerance. With this purpose we used 20 isogenic lines of
wheat. In each one of these isogenic lines only a chromosome from
‘Bezostaya’ variety (a winter habit cultivar) was substituted to
‘Capple desprez’ variety. The plant materials were planted in
controlled conditions with 20º C and 16 h day length in moderately
cold areas of Iran at Karaj Agricultural Research Station in 2006-07
and the acclimation period was completed for about 4 weeks in a
cold room with 4º C. The cold hardiness of these isogenic lines was
measured by LT50 (the temperature in which 50% of the plants are
killed by freezing stress).The experimental design was completely
randomized block design (RCBD)with three replicates. The results
showed that chromosome 5A had a major effect on freezing
tolerance, and then chromosomes 1A and 4A had less effect on this
trait. Further studies are essential to understanding the importance of
each chromosome in controlling cold hardiness in wheat.", keywords = "Cold hardiness, isogenic lines, LT50 ,Triticum.", volume = "3", number = "1", pages = "38-3", }