Abstract: Stress of slaughter animals starting long before until at the time of process of slaughtering which cause misery and decrease of meat quality. Meanwhile, determination of animal stress using hormonal such as cortisol is expensive and less practical so that portable stress indicator for cows based on Fourier Transform Infrared Spectroscopy (FTIR) must be provided. The aims of this research are to find out the comparison process of slaughter between Rope Casting Local (RCL) and Restraining Box Method (RBM) by measuring of cortisol and wavelength in FTIR methods. Thirty two of male Ongole crossbred cattle were used in this experiment. Blood sampling was taken from jugular vein when they were rested and repeated when slaughtered. All of blood samples were centrifuged at 3000 rpm for 20 minutes to get serum, and then divided into two parts for cortisol assayed using ELISA and for measuring the wavelength using FTIR. The serum then measured at the wavelength between 4000-400 cm-1 using MB3000 FTIR. Band data absorption in wavelength of FTIR is analyzed descriptively by using FTIR Horizon MBTM. For RCL, average of serum cortisol when the animals rested were 11.47 ± 4.88 ng/mL, when the time of slaughter were 23.27 ± 7.84 ng/mL. For RBM, level of cortisol when rested animals were 13.67 ± 3.41 ng/mL and 53.47 ± 20.25 ng/mL during the slaughter. Based on student t-Test, there were significantly different between RBM and RCL methods when beef cattle were slaughtered (P0.05). Result of FTIR with the various of wavelength such as methyl group (=CH3 ) 2986cm-1, methylene (=CH2 ) 2827 cm-1, hydroxyl (- OH) 3371 cm-1, carbonyl (ketones) (C=O) 1636 cm-1, carboxyl (COO-1) 1408 cm-1, glucosa 1057 cm-1, urea 1011 cm-1have been obtained. It can be concluded that the RCL slaughtered method is better than the RBM method based on the increase of cortisol as an indicator of stress in beef cattle (P
Abstract: This paper presented a study of three algorithms, the
equalization algorithm to equalize the transmission channel with ZF
and MMSE criteria, application of channel Bran A, and adaptive
filtering algorithms LMS and RLS to estimate the parameters of the
equalizer filter, i.e. move to the channel estimation and therefore
reflect the temporal variations of the channel, and reduce the error in
the transmitted signal. So far the performance of the algorithm
equalizer with ZF and MMSE criteria both in the case without noise,
a comparison of performance of the LMS and RLS algorithm.
Abstract: Hybrid electric vehicles can reduce pollution and
improve fuel economy. Power-split hybrid electric vehicles (HEVs)
provide two power paths between the internal combustion engine
(ICE) and energy storage system (ESS) through the gears of an
electrically variable transmission (EVT). EVT allows ICE to operate
independently from vehicle speed all the time. Therefore, the ICE can
operate in the efficient region of its characteristic brake specific fuel
consumption (BSFC) map. The two-mode powertrain can operate in
input-split or compound-split EVT modes and in four different fixed
gear configurations. Power-split architecture is advantageous because
it combines conventional series and parallel power paths. This
research focuses on input-split and compound-split modes in the
two-mode power-split powertrain. Fuzzy Logic Control (FLC) for an
internal combustion engine (ICE) and PI control for electric machines
(EMs) are derived for the urban driving cycle simulation. These
control algorithms reduce vehicle fuel consumption and improve ICE
efficiency while maintaining the state of charge (SOC) of the energy
storage system in an efficient range.
Abstract: Presently a significant portion of the Earth's
population does not have access to healthy food. Either because they
cannot afford it or because they do not know which one are they. The
aim of the VII th Framework Chance project (Nr. 266331) supported
by the European Union has been to develop relatively cheap food
with favourable nutritional value and it should have acceptable
quality for consumers. As one task of the project we manufactured
bread products as a basic food. We examined the enrichment of bread
products with four kinds of bran, with a special milling product of
grain industry (aleurone-rich flour) and with a soy-based sprouted
additive. The applied concentration of the six mentioned additives
has been optimized and the physical properties of the bread products
were monitored. The weight/density of the enriched breads increased
a bit, however the volume and height decreased slightly compared to
the corresponding data of the control bread. The optimized
composition of the final product is favourably affected by these
additives having highly preferred composition from nutritional point
of view.
Abstract: In dynamic system theory a mathematical model is
often used to describe their properties. In order to find a transfer
matrix of a dynamic system we need to calculate an inverse matrix.
The paper contains the fusion of the classical theory and the
procedures used in the theory of automated control for calculating the
inverse matrix. The final part of the paper models the given problem
by the Matlab.
Abstract: In this paper we propose a computer-aided solution
with Genetic Algorithms in order to reduce the drafting of reports:
FMEA analysis and Control Plan required in the manufacture of the
product launch and improved knowledge development teams for
future projects. The solution allows to the design team to introduce
data entry required to FMEA. The actual analysis is performed using
Genetic Algorithms to find optimum between RPN risk factor and
cost of production. A feature of Genetic Algorithms is that they are
used as a means of finding solutions for multi criteria optimization
problems. In our case, along with three specific FMEA risk factors is
considered and reduce production cost. Analysis tool will generate
final reports for all FMEA processes. The data obtained in FMEA
reports are automatically integrated with other entered parameters in
Control Plan. Implementation of the solution is in the form of an
application running in an intranet on two servers: one containing
analysis and plan generation engine and the other containing the
database where the initial parameters and results are stored. The
results can then be used as starting solutions in the synthesis of other
projects. The solution was applied to welding processes, laser cutting
and bending to manufacture chassis for buses. Advantages of the
solution are efficient elaboration of documents in the current project
by automatically generating reports FMEA and Control Plan using
multiple criteria optimization of production and build a solid
knowledge base for future projects. The solution which we propose is
a cheap alternative to other solutions on the market using Open
Source tools in implementation.
Abstract: Exploration and exploitation capabilities are both
important within Operations as means for improvement when
managed separately, and for establishing dynamic improvement
capabilities when combined in balance. However, it is unclear what
exploration and exploitation capabilities imply in improvement and
development work within an Operations context. So, in order to
better understand how to develop exploration and exploitation
capabilities within Operations, the main characteristics of these
constructs needs to be identified and further understood. Thus, the
objective of this research is to increase the understanding about
exploitation and exploration characteristics, to concretize what they
translates to within the context of improvement and development
work in an Operations unit, and to identify practical challenges. A
literature review and a case study are presented. In the literature
review, different interpretations of exploration and exploitation are
portrayed, key characteristics have been identified, and a deepened
understanding of exploration and exploitation characteristics is
described. The case in the study is an Operations unit, and the aim is
to explore to what extent and in what ways exploration and
exploitation activities are part of the improvement structures and
processes. The contribution includes an identification of key
characteristics of exploitation and exploration, as well as an
interpretation of the constructs. Further, some practical challenges are
identified. For instance, exploration activities tend to be given low
priority, both in daily work as in the manufacturing strategy. Also,
the overall understanding about the concepts of exploitation and
exploration (or any similar aspect of dynamic improvement
capabilities) is very low.
Abstract: In the immunologic sense, clinical infection is a state
of failure of the immune system to combat the pathogenic weapon of
the bacteria invading the host. A motile gram negative vibroid
organism associated with marked mono and poly nuclear cell
responses was traced during the examination of a clinical material
from an infected common carp Cyprinus carpio. On primary plate
culture, growth was shown to be pure, dense population of an
Aeromonas-like colony morphotype. The pure isolate was found to
be; Aerobic, facultatively anaerobic, non-halophilic, grew at 0C, and
37C, oxidase positive utilizes glucose through fermentative pathway,
resist 0/129 and novobiocin, produces alanine and lysine
decarboxylases but non-producing ornithine dehydrolases. Tests for
the in vitro determinants of pathogenicity has shown to be; Betahaemolytic
onto blood agar, gelatinase, casienase and amylase
producer. Three in vivo determinants of pathogenicity were tested as,
the lethal dose fifty, the pathogenesis and pathogenicity. It was
evident that 0.1 milliliter of the causal bacterial cell suspension of a
density 1 x 107 CFU/ml injected intramuscularly into an average of
100gms fish toke five days incubation period, then at the day six
morbidity and mortality were initiated. LD50 was recorded at the day
12 post-infection. Use of an LD50 doses to study the pathogenicity,
reveals mononuclear and polynuclear cell responses, on examining
the stained direct films of the clinical materials from the
experimentally infected fish. Re-isolation tests confirm that the reisolant
is same. The course of the infection in natural case was shown
manifestation of; skin ulceration, haemorrhage and descaling. On
evisceration, the internal organs were shown; congestion in the
intestines, spleen and, air sacs. The induced infection showed a
milder form of these manifestations. The grading of the virulence of
this organism was virulent causing chronic course of infections as
indicated from the pathogenesis and pathogenicity studies. Thus the
infectious bacteria were consistent with Aeromonas hydrophila, and
the infection was chronic.
Abstract: More than 3000 plants of notable phyto-therapeutic
value grow in South Africa; these include Cissampelos capensis,
commonly known in Afrikaans as dawidjie or dawidjiewortel. C.
capensis is the most significant and popular medicinal plant used by
the Khoisan as well as other rural groups in the Western region of
South Africa. Its rhizomes are traditionally used to treat male fertility
problems. Yet, no studies have investigated the effects of this plant or
its extracts on human spermatozoa. Therefore, this study aimed at
investigating the effects of C. capensis rhizome extract (CRE)
fractions on ejaculated human spermatozoa in vitro. Spermatozoa
from a total of 77 semen samples were washed with human tubular
fluid medium supplemented with bovine serum albumin (HTF-BSA)
and incubated for 2 hours with 20 μg/ml progesterone (P4) followed
by incubation with different concentrations (0, 0.05, 0.5, 5, 50, 200
μg/ml) of fractionated CRE (F1=0% MeOH, F2=30% MeOH,
F3=60% MeOH and F4=100% MeOH) for 1.5 hours at 37°C. A
sample without addition of CRE fractions served as control. Samples
were analyzed for sperm motility, reactive oxygen species (ROS),
DNA-fragmentation, acrosome reaction and capacitation. Results
showed that F1 resulted in significantly higher values for ROS,
capacitation and hyper-activation compared to F2, F3, and F4 with
P4-stimulated samples generally having higher values. No significant
effect was found for the other parameters. In conclusion, alkaloids
present in F1 of CRE appear to have triggered sperm intrinsic ROS
production leading to sperm capacitation and acrosome reaction
induced by P4.
Abstract: Steady three-dimensional and two free surface waves
generated by moving bodies are presented, the flow problem to be
simulated is rich in complexity and poses many modeling challenges
because of the existence of breaking waves around the ship hull, and
because of the interaction of the two-phase flow with the turbulent
boundary layer. The results of several simulations are reported. The
first study was performed for NACA0012 of hydrofoil with different
meshes, this section is analyzed at h/c= 1, 0345 for 2D. In the second
simulation a mathematically defined Wigley hull form is used to
investigate the application of a commercial CFD code in prediction of
the total resistance and its components from tangential and normal
forces on the hull wetted surface. The computed resistance and wave
profiles are used to estimate the coefficient of the total resistance for
Wigley hull advancing in calm water under steady conditions. The
commercial CFD software FLUENT version 12 is used for the
computations in the present study. The calculated grid is established
using the code computer GAMBIT 2.3.26. The shear stress k-ωSST
model is used for turbulence modeling and the volume of fluid
technique is employed to simulate the free-surface motion. The
second order upwind scheme is used for discretizing the convection
terms in the momentum transport equations, the Modified HRIC
scheme for VOF discretization. The results obtained compare well
with the experimental data.
Abstract: Whey is the lactose rich by-product of the dairy
industry, having good amount of nutrient reservoir. Most abundant
nutrients are lactose, soluble proteins, lipids and mineral salts.
Disposing of whey by most of milk plants which do not have proper
pre-treatment system is the major issue. As a result of which, there
can be significant loss of potential food and energy source. Thus,
whey has been explored as the substrate for the synthesis of different
value added products such as enzymes. β-galactosidase is one of the
important enzymes and has become the major focus of research due
to its ability to catalyze both hydrolytic as well as
transgalactosylation reaction simultaneously. The enzyme is widely
used in dairy industry as it catalyzes the transformation of lactose to
glucose and galactose, making it suitable for the lactose intolerant
people. The enzyme is intracellular in both bacteria and yeast,
whereas for molds, it has an extracellular location. The present work
was carried to utilize the whey for the production of β-galactosidase
enzyme using both yeast and fungal cultures. The yeast isolate
Kluyveromyces marxianus WIG2 and various fungal strains have
been used in the present study. Different disruption techniques have
also been investigated for the extraction of the enzyme produced
intracellularly from yeast cells. Among the different methods tested
for the disruption of yeast cells, SDS-chloroform showed the
maximum β-galactosidase activity. In case of the tested fungal
cultures, Aureobasidium pullulans NCIM 1050 was observed to be
the maximum extracellular enzyme producer.
Abstract: This paper introduces a video sharing platform based
on WiFi, which consists of camera, mobile phone and PC server. This
platform can receive wireless signal from the camera and show the live
video on the mobile phone captured by camera. In addition, it is able to
send commands to camera and control the camera’s holder to rotate.
The platform can be applied to interactive teaching and dangerous
area’s monitoring and so on. Testing results show that the platform can
share the live video of mobile phone. Furthermore, if the system’s PC
server and the camera and many mobile phones are connected
together, it can transfer photos concurrently.
Abstract: The present study was aimed to examine the structure
of children’s adaptation during school transition and to identify a
commonality and dissimilarity at the elementary and junior high
school. 1,983 students in the 6th grade and 2,051 students in the 7th
grade were extracted by stratified two-stage random sampling and
completed the ASSESS that evaluated the school adaptation from the
view point of ‘general satisfaction’, ‘teachers’ support’, ‘friends’
support’, ‘anti-bullying relationship’, ‘prosocial skills’, and ‘academic
adaptation’. The 7th graders tend to be worse adaptation than the 6th
graders. A structural equation modeling showed the goodness of fit for
each grades. Both models were very similar but the 7th graders’ model
showed a lower coefficient at the pass from ‘teachers’ support’ to
‘friends’ support’. The role of ‘teachers’ support’ was decreased to
keep a good relation in junior high school. We also discussed how we
provide a continuous assistance for prevention of the 7th graders’ gap.
Abstract: Methicillin/multiple-resistant Staphylococcus aureus
(MRSA) are infectious bacteria that are resistant to common
antibiotics. A previous in silico study in our group has identified a
hypothetical protein SAV1226 as one of the potential drug targets. In
this study, we reported the bioinformatics characterization, as well as
cloning, expression, purification and kinetic assays of hypothetical
protein SAV1226 from methicillin/vancomycin-resistant
Staphylococcus aureus Mu50 strain. MALDI-TOF/MS analysis
revealed a low degree of structural similarity with known proteins.
Kinetic assays demonstrated that hypothetical protein SAV1226 is
neither a domain of an ATP dependent dihydroxyacetone kinase nor
of a phosphotransferase system (PTS) dihydroxyacetone kinase,
suggesting that the function of hypothetical protein SAV1226 might
be misannotated on public databases such as UniProt and
InterProScan 5.
Abstract: Steady three-dimensional and two free surface waves
generated by moving bodies are presented, the flow problem to be
simulated is rich in complexity and poses many modeling challenges
because of the existence of breaking waves around the ship hull, and
because of the interaction of the two-phase flow with the turbulent
boundary layer. The results of several simulations are reported. The
first study was performed for NACA0012 of hydrofoil with different
meshes, this section is analyzed at h/c= 1, 0345 for 2D. In the second
simulation a mathematically defined Wigley hull form is used to
investigate the application of a commercial CFD code in prediction of
the total resistance and its components from tangential and normal
forces on the hull wetted surface. The computed resistance and wave
profiles are used to estimate the coefficient of the total resistance for
Wigley hull advancing in calm water under steady conditions. The
commercial CFD software FLUENT version 12 is used for the
computations in the present study. The calculated grid is established
using the code computer GAMBIT 2.3.26. The shear stress k-ωSST
model is used for turbulence modeling and the volume of fluid
technique is employed to simulate the free-surface motion. The
second order upwind scheme is used for discretizing the convection
terms in the momentum transport equations, the Modified HRIC
scheme for VOF discretization. The results obtained compare well
with the experimental data.
Abstract: Nowadays, the main goal for modern horticultural
production is an increase the quality. In recent years, the use of
organic fertilizers or biostimulants that can be applied in agriculture
to improve quali-quantitative crop yields has encountered increasing
interest. Biostimulants are gaining importance also for their possible
use in organic and sustainable agriculture, to avoid excessive
fertilizer applications. Consecutive experimental trials were carried
out in the Apulia region (southern Italy) on three herbaceous crops
(cauliflower, pepper, fennel) grown in pots under conventional and
organic fertilization systems without and with biostimulants. The aim
was to determine the effects of three biostimulants (Siapton®10L,
Micotech L, Lysodin Alga-Fert) on quali-quantitative yield
characteristics. At harvest, the quali-quantitative yield characteristics
of each crop were determined. All of the experimental data were
subjected to analysis of variance (ANOVA), and when significant
effects were detected, the means were compared using Tukey’s tests.
These data show large differences in these yield characteristics
between conventional and organic crops, particularly highlighting
higher yields for the conventional crops, while variable results were
generally observed when the biostimulants were applied. In this
context, there were no effects of the biostimulants on the quantitative
yield, whereas there were low positive effects on the qualitative
characteristics, as related to higher dry matter content of cauliflower,
and higher soluble solids content of pepper. Moreover, there were
evident positive effects of the biostimulants with fennel, due to the
lower nitrate content. These latter data are in line with most of the
published literature obtained for other herbaceous crops.
Abstract: The nanoindentation behaviour and phase
transformation of annealed single-crystal silicon wafers are examined.
The silicon specimens are annealed at temperatures of 250, 350 and
450ºC, respectively, for 15 minutes and are then indented to maximum
loads of 30, 50 and 70 mN. The phase changes induced in the indented
specimens are observed using transmission electron microscopy
(TEM) and micro-Raman scattering spectroscopy (RSS). For all
annealing temperatures, an elbow feature is observed in the unloading
curve following indentation to a maximum load of 30 mN. Under
higher loads of 50 mN and 70 mN, respectively, the elbow feature is
replaced by a pop-out event. The elbow feature reveals a complete
amorphous phase transformation within the indented zone, whereas
the pop-out event indicates the formation of Si XII and Si III phases.
The experimental results show that the formation of these crystalline
silicon phases increases with an increasing annealing temperature and
indentation load. The hardness and Young’s modulus both decrease as
the annealing temperature and indentation load are increased.
Abstract: Current research is targeting new molecular
mechanisms that underlie non-alcoholic fatty liver disease (NAFLD)
and associated metabolic disorders like non-alcoholic steatohepatitis
(NASH). Forty New Zealand White rabbits have been used and fed a
high protein (HP) and energy diet based on grains and containing
11.76 MJ/kg. Boron added to 3 experimental groups’ drinking waters
(30 mg boron/L) as boron compounds. Biochemical analysis
including boron levels, and nuclear magnetic resonance (NMR) based
metabolomics evaluation, and mRNA expression of peroxisome
proliferator-activated receptor (PPAR) family was performed. LDLcholesterol
concentrations alone were decreased in all the
experimental groups. Boron levels in serum and feces were increased.
Content of acetate was in about 2x higher for anhydrous borax group,
at least 3x higher for boric acid group. PPARα mRNA expression
was significantly decreased in boric acid group. Anhydrous borax
attenuated mRNA levels of PPARγ, which was further suppressed by
boric acid. Boron supplementation decreased the degenerative
alterations in hepatocytes. Except borax group other boron groups did
not have a pronounced change in tubular epithels of kidney. In
conclusion, high protein and energy diet leads hepatocytes’
degenerative changes which can be prevented by boron
supplementation. Boric acid seems to be more effective in this
situation.
Abstract: The popularity of quality management system models
continues to grow despite the transitional crisis in 2008. Their
development is associated with demands of the new requirements for
entrepreneurs, such as risk analysis projects and more emphasis on
supervision of outsourced processes. In parallel, it is appropriate to
focus attention on the selection of companies aspiring to a quality
management system. This is particularly important in the automotive
supplier industry, where requirements transferred to the levels in the
supply chain should be clear, transparent and fairly satisfied. The
author has carried out a series of researches aimed at finding the
factors that allow for the effective implementation of the quality
management system in automotive companies. The research was
focused on four groups of companies: 1) manufacturing (parts and
assemblies for the purpose of sale or for vehicle manufacturers), 2)
service (repair and maintenance of the car) 3) services for the
transport of goods or people, 4) commercial (auto parts and vehicles).
The identified determinants were divided into two types of criteria:
internal and external, as well as hard and soft. The article presents the
hard – technical factors that an automotive company must meet in
order to achieve the goal of the quality management system
implementation.
Abstract: This article proposes a new method for application in
communication circuit systems that increase efficiency, PAE, output
power and gain in the circuit. The proposed method is based on a
combination of switching class-E and class-J and has been termed
class-EJ. This method was investigated using both theory and
simulation to confirm ∼72% PAE and output power of >39dBm. The
combination and design of the proposed power amplifier accrues gain
of over 15dB in the 2.9 to 3.5GHz frequency bandwidth. This circuit
was designed using MOSFET and high power transistors. The loadand
source-pull method achieved the best input and output networks
using lumped elements. The proposed technique was investigated for
fundamental and second harmonics having desirable amplitudes for
the output signal.