Abstract: Since polymerase chain reaction (PCR) has been
invented, it has emerged as a powerful tool in genetic analysis. The
PCR products are closely linked with thermal cycles. Therefore, to
reduce the reaction time and make temperature distribution uniform in
the reaction chamber, a novel oscillatory thermal cycler is designed.
The sample is placed in a fixed chamber, and three constant isothermal
zones are established and lined in the system. The sample is oscillated
and contacted with three different isothermal zones to complete
thermal cycles. This study presents the design of the geometric
characteristics of the chamber. The commercial software
CFD-ACE+TM is utilized to investigate the influences of various
materials, heating times, chamber volumes, and moving speed of the
chamber on the temperature distributions inside the chamber. The
chamber moves at a specific velocity and the boundary conditions
with time variations are related to the moving speed. Whereas the
chamber moves, the boundary is specified at the conditions of the
convection or the uniform temperature. The user subroutines compiled
by the FORTRAN language are used to make the numerical results
realistically. Results show that the reaction chamber with a rectangular
prism is heated on six faces; the effects of various moving speeds of
the chamber on the temperature distributions are examined. Regarding
to the temperature profiles and the standard deviation of the
temperature at the Y-cut cross section, the non-uniform temperature
inside chamber is found as the moving speed is larger than 0.01 m/s.
By reducing the heating faces to four, the standard deviation of the
temperature of the reaction chamber is under 1.4×10-3K with the range
of velocities between 0.0001 m/s and 1 m/s. The nature convective
boundary conditions are set at all boundaries while the chamber moves
between two heaters, the effects of various moving velocities of the
chamber on the temperature distributions are negligible at the assigned
time duration.
Abstract: Supplementation of palm vitamin E has been reported
to prevent loss of bone density in ovariectomised female rats. The
mechanism by which palm vitamin E exerts these effects is still
unknown. We hypothesized that palm vitamin E may act by
preventing the protein expression changes. Two dimensional poly
acyrilamide gel electrophoresis (2-D PAGE) and PD Quest software
genomic solutions Investigator (proteomics) was used to analyze the
differential protein expression profile in femoral and humeri bones
harvested from three groups of rats; sham-operated rats (SO),
ovariectomised rats (Ovx) and ovariectomised rats supplemented for
2 months with palm vitamin E. The results showed that there were
over 300 valued spot on each of the groups PVE and OVX as
compared to about 200 in SO. Comparison between the differential
protein expression between OVX and PVE groups showed that ten
spots were down –regulated in OVX but up-regulated in PVE. The
ten differential spots were separately named P1-P10. The
identification and understanding of the pathway of the differential
protein expression among the groups is ongoing and may account for
the molecular mechanism through which palm vitamin E exert its
anti-osteoporotic effect.
Abstract: Acute disseminated encephalomyelitis (ADEM) has
been reported to develop after a hymenoptera sting, but its
pathogenesis is not known in detail. Myelin basic protein (MBP)-
specific T cells have been detected in the blood of patients with
ADEM, and a proportion of these patients develop multiple sclerosis
(MS). In an attempt to understand the mechanisms underlying
ADEM, molecular mimicry between hymenoptera venom peptides
and the human immunodominant MBP peptide was scrutinized,
based on the sequence and structural similarities, whether it was the
root of the disease. The results suggest that the three wasp venom
peptides have low sequence homology with the human
immunodominant MBP residues 85-99. Structural similarity analysis
among the three venom peptides and the MS-related HLA-DR2b
(DRA, DRB1*1501)-associated immunodominant MHC
binding/TCR contact residues 88-93, VVHFFK showed that
hyaluronidase residues 7-12, phospholipase A1 residues 98-103, and
antigen 5 residues 109-114 showed a high degree of similarity
83.3%, 100%, and 83.3% respectively. In conclusion, some wasp
venom peptides, particularly phospholipase A1, may potentially act
as the molecular motifs of the human 3HLA-DR2b-associated
immunodominant MBP88-93, and possibly present a mechanism for
induction of wasp sting-associated ADEM.
Abstract: Presented herein is an assessment of current nonlinear
static procedures (NSPs) for seismic evaluation of bucklingrestrained
braced frames (BRBFs) which have become a favorable
lateral-force resisting system for earthquake resistant buildings. The
bias and accuracy of modal, improved modal pushover analysis
(MPA, IMPA) and mass proportional pushover (MPP) procedures
are comparatively investigated when they are applied to BRBF
buildings subjected to two sets of strong ground motions. The
assessment is based on a comparison of seismic displacement
demands such as target roof displacements, peak floor/roof
displacements and inter-story drifts. The NSP estimates are compared
to 'exact' results from nonlinear response history analysis (NLRHA).
The response statistics presented show that the MPP
procedure tends to significantly overestimate seismic demands of
lower stories of tall buildings considered in this study while MPA
and IMPA procedures provide reasonably accurate results in
estimating maximum inter-story drift over all stories of studied BRBF
systems.
Abstract: India recognizes the personal laws of the various
religious communities that reside in the country. At the same time all
the institutions of the state in India are committed to the value of
secularism. This paper has been developed on the basis of a case
study that indicates the dynamics of religion in the working of the
lower judiciary in India. Majority of the commentary on religion and
the judiciary has focused on debates surrounding the existence and
application of personal laws. This paper, through a case study in the
lower judiciary, makes an attempt to examine whether the interface
between religion and the judiciary goes beyond personal laws.
The first part of this paper explains the history and application of
personal laws in social, political and legal contexts in India. The
second part examines the case study located in two courts of first
instance, following into the third part which provides an analysis of
the empirical evidence. The fourth part focuses on preliminary
observations about why there is a hesitancy to speak about religion in
relation to the working of the judicial system.
Abstract: The development of the poultry industry in Albania is mainly based on the existence of intensive modern farms with huge capacities, which often are mixed with other forms. Colibacillosis is commonly displayed regardless of the type of breeding, delivering high mortality in poultry industry. The mechanisms with which pathogen enterobacters are able to cause the infection in poultry are not yet clear. The routine diagnose in the field, followed by isolation of E. coli and species of Salmonella genres in reference laboratories cannot lead in classification or full recognition of circulative strains in a territory, if it is not performed a differentiation among the present microorganisms in intensive farms and those in rural areas. In this study were isolated 1.496 strains of E. coli and 378 Salmonella spp. This study, presents distribution of poultry pathogenosity of E.coli and Salmonella spp., based on the usage of innovative diagnostic methods.
Abstract: The evolutionary design of electronic circuits, or
evolvable hardware, is a discipline that allows the user to
automatically obtain the desired circuit design. The circuit
configuration is under the control of evolutionary algorithms. Several
researchers have used evolvable hardware to design electrical
circuits. Every time that one particular algorithm is selected to carry
out the evolution, it is necessary that all its parameters, such as
mutation rate, population size, selection mechanisms etc. are tuned in
order to achieve the best results during the evolution process. This
paper investigates the abilities of evolution strategy to evolve digital
logic circuits based on programmable logic array structures when
different mutation rates are used. Several mutation rates (fixed and
variable) are analyzed and compared with each other to outline the
most appropriate choice to be used during the evolution of
combinational logic circuits. The experimental results outlined in this
paper are important as they could be used by every researcher who
might need to use the evolutionary algorithm to design digital logic
circuits.
Abstract: The results show that the bridge equipped with seismic isolation bearing system shows a high amount of energy dissipation. The purpose of the present study is to analyze the overall performance of continuous curved highway viaducts with different bearing supports, with an emphasis on the effectiveness of seismic isolation based on lead rubber bearing and hedge reaction force bearing system consisted of friction sliding bearing and rubber bearing. The bridge seismic performance has been evaluated on six different cases with six bearing models. The effects of the different arrangement of bearing on the deck superstructure displacements, the seismic damage at the bottom of the piers, movement track at the pier-s top and the total and strain energies absorbed by the structure are evaluated. In conclusion, the results provide sufficient evidence of the effectiveness on the use of seismic isolation on steel curved highway bridges.
Abstract: In this study acoustic emission (AE) signals obtained during deformation and fracture of two types of ferrite-martensite dual phase steels (DPS) specimens have been analyzed in frequency domain. For this reason two low carbon steels with various amounts of carbon were chosen, and intercritically heat treated. In the introduced method, identifying the mechanisms of failure in the various phases of DPS is done. For this aim, AE monitoring has been used during tensile test of several DPS with various volume fraction of the martensite (VM) and attempted to relate the AE signals and failure mechanisms in these steels. Different signals, which referred to 2-3 micro-mechanisms of failure due to amount of carbon and also VM have been seen. By Fast Fourier Transformation (FFT) of signals in distinct locations, an excellent relationship between peak frequencies in these areas and micro-mechanisms of failure were seen. The results were verified by microscopic observations (SEM).
Abstract: The influence of physical (external added weight) and
neurophysiological (fatigue) factors on static and dynamic balance in
sport related activities was typified statically by the Romberg test
(one foot flat, eyes open) and dynamically by jumping and hopping
in both horizontal and vertical directions. Twenty healthy males were
participated in this study. In Static condition, added weight increased
body-s inertia and therefore decreased body sway in AP direction
though not significantly. Dynamically, added weight significantly
increased body sway in both ML and AP directions, indicating
instability, and the use of the counter rotating segments mechanism to
maintain balance was demonstrated. Fatigue on the other hand
significantly increased body sway during static balance as a
neurophysiological adaptation primarily to the inverted pendulum
mechanism. Dynamically, fatigue significantly increased body sway
in both ML and AP directions again indicating instability but with a
greater use of counter rotating segments mechanism. Differential
adaptations for each of the two balance mechanisms (inverted
pendulum and counter rotating segments) were found between one
foot flat and two feet flat dynamic conditions, as participants relied
more heavily on the first in the one foot flat conditions and relied
more on the second in the two feet flat conditions.
Abstract: Healthcare providers sometimes use the power of
humor as a treatment and therapy for buffering mental health or easing
mental disorders because humor can provide relief from distress and
conflict. Humor is also very suitable for advertising because of similar
benefits. This study carefully examines humor's widespread use in
advertising and identifies relationships among humor mechanisms,
female depictions, and product types. The purpose is to conceptualize
how humor theories can be used not only to successfully define a
product as fitting within one of four color categories of the product
color matrix, but also to identify compelling contemporary female
depictions through humor in ads. The results can offer an idealization
for marketing managers and consumers to help them understand how
female role depictions can be effectively used with humor in ads. The
four propositions developed herein are derived from related literature,
through the identification of marketing strategy formulations that
achieve product memory enhancement by adopting humor
mechanisms properly matched with female role depictions.
Abstract: Government of Indonesia held a certification program to enhance the professionalism of teachers by using portfolio assessment. This research discusses about the effectiveness of certification programs to enhance the professionalism of teacher in Indonesia. Portfolio assessment method has drawbacks. The certified teachers do not show significant performance improvement. Therefore, the government changes the portfolio assessment method to the education and training for teachers.
Abstract: In the various working field, vibration may cause injurious to human body. Especially, in case of the vibration which is constantly and repeatedly transferred to the human. That gives serious physical problem, so called, Reynaud phenomenon. In this paper, we propose a vibration transmissibility reduction module with flexure mechanism for personal tools. At first, we select a target personal tool, grass cutter, and measure the level of vibration transmissibility on the hand. And then, we develop the concept design of the module that has stiffness for reduction the vibration transmissibility more than 20%, where the vibration transmissibility is measured with an accelerometer. In addition, the vibration reduction can be enhanced when the interior gap between inner and outer body is filled with silicone gel. This will be verified by the further experiment.
Abstract: In this study, two new classes of generalized homeomorphisms are introduced and shown that one of these classes has a group structure. Moreover, some properties of these two homeomorphisms are obtained.
Abstract: During last decades, worldwide researchers dedicated
efforts to develop machine-based seismic Early Warning systems,
aiming at reducing the huge human losses and economic damages.
The elaboration time of seismic waveforms is to be reduced in order
to increase the time interval available for the activation of safety
measures. This paper suggests a Data Mining model able to correctly
and quickly estimate dangerousness of the running seismic event.
Several thousand seismic recordings of Japanese and Italian
earthquakes were analyzed and a model was obtained by means of a
Bayesian Network (BN), which was tested just over the first
recordings of seismic events in order to reduce the decision time and
the test results were very satisfactory.
The model was integrated within an Early Warning System
prototype able to collect and elaborate data from a seismic sensor
network, estimate the dangerousness of the running earthquake and
take the decision of activating the warning promptly.
Abstract: The implementation of single-electron tunneling
(SET) simulators based on the master-equation (ME) formalism
requires the efficient and accurate identification of an exhaustive list
of active states and related tunnel events. Dynamic simulations also
require the control of the emerging states and guarantee the safe
elimination of decaying states. This paper describes algorithms for
use in the stationary and dynamic control of the lists of active states
and events. The paper presents results obtained using these
algorithms with different SET structures.
Abstract: The automatic discrimination of seismic signals is an important practical goal for earth-science observatories due to the large amount of information that they receive continuously. An essential discrimination task is to allocate the incoming signal to a group associated with the kind of physical phenomena producing it. In this paper, two classes of seismic signals recorded routinely in geophysical laboratory of the National Center for Scientific and Technical Research in Morocco are considered. They correspond to signals associated to local earthquakes and chemical explosions. The approach adopted for the development of an automatic discrimination system is a modular system composed by three blocs: 1) Representation, 2) Dimensionality reduction and 3) Classification. The originality of our work consists in the use of a new wavelet called "modified Mexican hat wavelet" in the representation stage. For the dimensionality reduction, we propose a new algorithm based on the random projection and the principal component analysis.
Abstract: The Post colonial society in India has witnessed the turmoil to come out from the widespread control and influence of colonialism. The socio-cultural life of a society with all its dynamics is reflected in realistic forms of literature. The social events and human experience are drawn into a new creative form and are given to the reader as a new understanding and perspective of life. It enables the reader to understand the essence of life and motivates him to prepare for a positive change. After India becoming free from the colonial rule in 1947, systematic efforts were made by central and state governments and institutions to limit the role of English and simultaneously enlarge the function of Indian languages by planning in a strategic manner. The eighteen languages recognized as national languages are having very rich literatures. Telugu language is one among the Dravidian language family and is widely spoken by a majority of people. The post colonial socio-cultural factors were very well reflected in Telugu literature. The anti-colonial, reform oriented, progressive, post modernistic trends in Telugu literature are nothing but creative reflections of the post colonial society. This paper examines the major socio-cultural reflections in Telugu literature of the post colonial period.
Abstract: Seasonal survey of freshwater snails in different water
courses in Egypt during two successive years included 13 snail
species. They represented by Biomphalaria alexandrina, Bulinus
truncatus, Physa acuta, Helisoma duryi, Lymnaea natalensis,
Planorbis pantries, Cleopatra bulimoides, Lanistes carinatus,
Bellamya unicolor, Melanoides tuberculata, Theodoxus niloticus,
Succinia cleopatra and Valvata nilotica. B. alexandrina was most
abundant during autumn and spring represented by 26and14
snails/site, respectively. B. truncatus was most abundant during
winter (7.7and3.6snails/site) of the two years, respectively. L.
natalensis was represented by 7snails/site in summer. The tolerance
of different snail species to the chemical elements was determined
seasonally and correlated to their abundance. In spring, autumn and
winter, B. alexandrina was significantly found to live under the
highest level of Pb, Cd,Cu, Na, K and Ca concentrations than the
other species (p
Abstract: Absorptive characteristics of polyaniline synthesized
in mixture of water and acetonitrile in 50/50 volume ratio was
studied. Synthesized polyaniline in powder shape is used as an
adsorbent to remove toxic hexavalent chromium from aqueous
solutions. Experiments were conducted in batch mode with different
variables such as agitation time, solution pH and initial concentration
of hexavalent chromium. Removal mechanism is the combination of
surface adsorption and reduction. The equilibrium time for removal
of Cr(T) and Cr(VI) was about 2 and 10 minutes respectively. The
optimum pH for total chromium removal occurred at pH 7 and
maximum hexavalent chromium removal took place under acidic
condition at pH 3. Investigating the isothermal characteristics showed
that the equilibrium adsorption data fitted both Freundlich-s and
Langmuir-s isotherms. The maximum adsorption of chromium was
calculated 36.1 mg/g for polyaniline