Abstract: In this study, the mechanical properties of alginate hydrogel material for self-standing 3D scaffold architecture with proper shape fidelity are investigated. In-lab built 3D bio-printer extrusion-based technology is utilized to fabricate 3D alginate scaffold constructs. The pressure, needle speed and stage speed are varied using a computer-controlled system. The experimental result indicates that the concentration of alginate solution, calcium chloride (CaCl2) cross-linking concentration and cross-linking ratios lead to the formation of alginate hydrogel with various gelation states. Besides, the gelling conditions, such as cross-linking reaction time and temperature also have a significant effect on the mechanical properties of alginate hydrogel. Various experimental tests such as the material gelation, the material spreading and the printability test for filament collapse as well as the swelling test were conducted to evaluate the fabricated 3D scaffold constructs. The result indicates that the fabricated 3D scaffold from composition of 3.5% wt alginate solution, that is prepared in DI water and 1% wt CaCl2 solution with cross-linking ratios of 7:3 show good printability and sustain good shape fidelity for more than 20 days, compared to alginate hydrogel that is prepared in a phosphate buffered saline (PBS). The fabricated self-standing 3D scaffold constructs measured 30 mm × 30 mm and consisted of 4 layers (n = 4) show good pore geometry and clear grid structure after printing. In addition, the percentage change of swelling degree exhibits high swelling capability with respect to time. The swelling test shows that the geometry of 3D alginate-scaffold construct and of the macro-pore are rarely changed, which indicates the capability of holding the shape fidelity during the incubation period. This study demonstrated that the mechanical and physical properties of alginate hydrogel could be tuned for a 3D bio-printing extrusion-based system to fabricate self-standing 3D scaffold soft structures. This 3D bioengineered scaffold provides a natural microenvironment present in the extracellular matrix of the tissue, which could be seeded with the biological cells to generate the desired 3D live tissue model for in vitro and in vivo tissue engineering applications.
Abstract: Calcium phosphate cement (CPC) is one of the most
attractive bioceramics due to its moldable and shape ability to fill
complicated bony cavities or small dental defect positions. In this
study, CPC was produced by using mixture of tetracalcium phosphate
(TTCP, Ca4O(PO4)2) and dicalcium phosphate anhydrous (DCPA,
CaHPO4) in equimolar ratio (1/1) with aqueous solutions of acetic
acid (C2H4O2) and disodium hydrogen phosphate dehydrate
(Na2HPO4.2H2O) in combination with sodium alginate in order to
improve theirs moldable characteristic. The concentration of the
aqueous solutions and sodium alginate were varied to investigate the
effect of different aqueous solutions and alginate on properties of the
cements. The cement paste was prepared by mixing cement powder
(P) with aqueous solution (L) in a P/L ratio of 1.0g/0.35ml. X-ray
diffraction (XRD) was used to analyses phase formation of the
cements. Setting time and compressive strength of the set CPCs were
measured using the Gilmore apparatus and Universal testing
machine, respectively.
The results showed that CPCs could be produced by using both
basic (Na2HPO4.2H2O) and acidic (C2H4O2) solutions. XRD results
show the precipitation of hydroxyapatite in all cement samples. No
change in phase formation among cements using difference
concentrations of Na2HPO4.2H2O solutions. With increasing
concentration of acidic solutions, samples obtained less
hydroxyapatite with a high dicalcium phosphate dehydrate leaded to
a shorter setting time. Samples with sodium alginate exhibited higher
crystallization of hydroxyapatite than that of without alginate as a
result of shorten setting time in a basic solution but a longer setting
time in an acidic solution. The stronger cement was attained from
samples using the acidic solution with sodium alginate; however the
strength was lower than that of using the basic solution.
Abstract: Oil entrapped floating alginate beads of curcumin were developed and characterized. Cremophor EL, Cremophor RH and Tween 80 were utilized to improve the solubility of the drug. The oil-loaded floating gel beads prepared by emulsion gelation method contained sodium alginate, mineral oil and surfactant. The drug content and % encapsulation declined as the ratio of surfactant was increased. The release of curcumin from 1% alginate beads was significantly more than for the 2% alginate beads. The drug released from the beads containing 25% of Tween 80 was about 70% while a higher drug release was observed with the beads containing Cremophor EL or Cremohor RH (approximately 90%). The developed floating beads of curcumin powder with surfactant provided a superior drug release than those without surfactant. Floating beads based on oil entrapment containing the drug solubilized in surfactants is a new delivery system to enhance the dissolution of poorly soluble drugs.
Abstract: The effects of coatings based on sodium alginate (S.A) and carboxyl methyl cellulose (CMC) on the color and moisture characteristics of potato round slices were investigated. It is the first time that this combination of polysaccharides is used as edible coating which alone had the best performance as inhibitor of potato color discoloration during the storage of 15 days at 4oC. When ascorbic acid (AA) and green tea (GT) were added in the above edible coating its effects on potato round slices changed. The mixtures of sodium alginate and carboxyl methyl cellulose with ascorbic acid or with green tea behave as a potential moisture barrier, resulting to the extent of potato samples self–life. These data suggests that both GT and AA are potential inhibitors of dehydration in potatoes and not only natural antioxidants.