Abstract: Australia, while being a large and eager consumer of
innovative and cutting edge Information and Communication
Technologies (ICT), continues to struggle to remain a leader in
Technological Innovation. This paper has two main contributions to
address certain aspects of this complex issue. The first being the
current findings of an ongoing research project on Information and
Innovation Management in the Australian Information and
Communication Technologies (ICT) sector. The major issues being
considered by the project include: investigation of the possible
inherent entrepreneurial nature of ICT; how to foster ICT innovation;
and examination of the inherent difficulties currently found within
the ICT industry of Australia in regards to supporting the
development of innovative and creative ideas. The second major
contribution is details of the I.-C.A.N. (Innovation by Collaborative
Anonymous Networking) software application information
management tool created and evolving in our research group. I-CAN,
besides having a positive reinforcement acronym, is aimed at
facilitating productive collaborative innovation in an Australian
workplace. Such a work environment is frequently subjected to
cultural influences such as the 'tall poppy syndrome' and 'negative'
or 'unconstructive' peer-pressure. There influences are frequently
seen as inhibitors to employee participation, entrepreneurship and
innovation.
Abstract: In this paper we study different similarity based approaches for the development of QSAR model devoted to the prediction of activity of antiobesity drugs. Classical similarity approaches are compared regarding to dissimilarity models based on the consideration of the calculation of Euclidean distances between the nonisomorphic fragments extracted in the matching process. Combining the classical similarity and dissimilarity approaches into a new similarity measure, the Approximate Similarity was also studied, and better results were obtained. The application of the proposed method to the development of quantitative structure-activity relationships (QSAR) has provided reliable tools for predicting of inhibitory activity of drugs. Acceptable results were obtained for the models presented here.
Abstract: The effects of coatings based on sodium alginate (S.A) and carboxyl methyl cellulose (CMC) on the color and moisture characteristics of potato round slices were investigated. It is the first time that this combination of polysaccharides is used as edible coating which alone had the best performance as inhibitor of potato color discoloration during the storage of 15 days at 4oC. When ascorbic acid (AA) and green tea (GT) were added in the above edible coating its effects on potato round slices changed. The mixtures of sodium alginate and carboxyl methyl cellulose with ascorbic acid or with green tea behave as a potential moisture barrier, resulting to the extent of potato samples self–life. These data suggests that both GT and AA are potential inhibitors of dehydration in potatoes and not only natural antioxidants.
Abstract: The mathematical modeling of different biological
processes is usually used to predict or assess behavior of systems in
which these processes take place. This study deals with mathematical
and computer modeling of bi-substrate enzymatic reactions with
ping-pong mechanism, which play an important role in different
biochemical pathways. Besides that, three models of competitive
inhibition were designed using different software packages. The main
objective of this study is to represent the results from in silico
investigation of bi-substrate enzymatic reactions with ordered pingpong
mechanism in the presence of competitive inhibitors, as well as
to describe in details the inhibition effects. The simulation of the
models with certain kinetic parameters allowed investigating the
behavior of reactions as well as determined some interesting aspects
concerning influence of different cases of competitive inhibition.
Simultaneous presence of two inhibitors, competitive to the S1 and S2
substrates have been studied. Moreover, we have found the pattern of
simultaneous influence of both inhibitors.
Abstract: In studying the possibility of using plants as
rhizoremediators, barley and grass mixture which showed resistance
to various concentrations of oil were selected. The minimum
inhibitory effect of oil on these plants by morphological parameters
such as survival of plants, length and biomass of shoot and root
compared with the control was showed. In determining physiological
parameters, a slight decrease in the number of chlorophyll a and b in
the leaves of plants was noted. The differences in the ratio of the total
surface of the roots to the work surface with the growth of plants in
soil with oil in the study of adsorption of the root surface were
showed.
Abstract: A gene network gives the knowledge of the regulatory
relationships among the genes. Each gene has its activators and
inhibitors that regulate its expression positively and negatively
respectively. Genes themselves are believed to act as activators and
inhibitors of other genes. They can even activate one set of genes and
inhibit another set. Identifying gene networks is one of the most
crucial and challenging problems in Bioinformatics. Most work done
so far either assumes that there is no time delay in gene regulation or
there is a constant time delay. We here propose a Dynamic Time-
Lagged Correlation Based Method (DTCBM) to learn the gene
networks, which uses time-lagged correlation to find the potential
gene interactions, and then uses a post-processing stage to remove
false gene interactions to common parents, and finally uses dynamic
correlation thresholds for each gene to construct the gene network.
DTCBM finds correlation between gene expression signals shifted in
time, and therefore takes into consideration the multi time delay
relationships among the genes. The implementation of our method is
done in MATLAB and experimental results on Saccharomyces
cerevisiae gene expression data and comparison with other methods
indicate that it has a better performance.
Abstract: Phytotoxicity of Daphne gnidium L. was evaluated
through the effect of incorporating leaves, stems and roots biomass
into soil (at 12.5, 25, 50g/Kg) and irrigation by their aqueous extracts
(50g/L), on the growth of two crops (Lactuca sativa L. and Raphanus
sativus L.) and two weeds (Peaganum harmala L. and Scolymus
maculatus L.). Results revealed a perceptible phytotoxic effect which
increased with dose and concentration. At the highest dose, roots and
leaves residues was the most toxic and caused total inhibition
respectively, for lettuce and thistle seedling growth. Irrigation with
aqueous extracts of D. gnidium different organs decreased also
seedlings length of all test species. Stems extract was more inhibitor
on thistle than peganum seedling growth; it induced a significant
reduction of 80% and 67%, for, respectively, roots and shoots.
Results of the present study suggest that different organs of D.
gnidium could be exploited in the management of agro-ecosystems.
Abstract: Vernonia divergens Benth., commonly known as
“Insulin Plant” (Fam: Asteraceae) is a potent sugar killer. Locally the
leaves of the plant, boiled in water are successfully administered to a
large number of diabetic patients. The present study evaluates the
putative anti-diabetic ingredients, isolated from the in vivo and in
vitro grown plantlets of V. divergens for their antimicrobial and
anticancer activities. Sterilized explants of nodal segments were
cultured on MS (Musashige and Skoog, 1962) medium in presence of
different combinations of hormones. Multiple shoots along with
bunch of roots were regenerated at 1mg l-1 BAP and 0.5 mg l-1 NAA.
Micro-plantlets were separated and sub-cultured on the double
strength (2X) of the above combination of hormones leading to
increased length of roots and shoots. These plantlets were
successfully transferred to soil and survived well in nature. The
ethanol extract of plantlets from both in vivo & in vitro sources were
prepared in soxhlet extractor and then concentrated to dryness under
reduced pressure in rotary evaporator. Thus obtainedconcentrated
extracts showed significant inhibitory activity against gram
negative bacteria like Escherichia coli and Pseudomonas
aeruginosa but no inhibition was found against gram positive
bacteria. Further, these ethanol extracts were screened for in vitro
percentage cytotoxicity at different time periods (24 h, 48 h and 72 h)
of different dilutions. The in vivo plant extract inhibited the growth of
EAC mouse cell lines in the range of 65, 66, 78, and 88% at 100, 50,
25 & 12.5μg mL-1 but at 72 h of treatment. In case of the extract of in
vitro origin, the inhibition was found against EAC cell lines even at
48h. During spectrophotometric scanning, the extracts exhibited
different maxima (ʎ) - four peaks in in vitro extracts as against single
in in vivo preparation suggesting the possible change in the nature of
ingredients during micropropagation through tissue culture
techniques.
Abstract: An experiment was conducted under controlled
conditions to study the effect of water extract of leaves, shoots and
roots of either Sisymbrium irio L. =SISIR and/or Descurainia
sophia (L.) Schur =DESSO on the germination and primary growth
of wheat. A split-split plot experiment in CRD with three
replications was used. The main plots were the type of weed: i.e.
SISIR and DESSO and the sub-plots were type of organ: i.e. leaf,
stem and root and, the sub-sub plots were concentration of the
water extract of each organ of the weeds: i.e. 0, 2, 4 and 8 % w/v.
The plant materials were cut in 2-3 cm pieces and then were
ground in a blender. The crushed materials were weighed
according to experimental protocol and the final volume was
reached to 100 ml in distilled water in dark bottles. All bottles were
put on a shaker for 24 hours. The solutions were filtered by muslin
cloth. Whatman paper, 9 cm in diameter, were put in petri dishes
and twenty seeds of wheat were put on it and 5 ml distilled water
or water extract of weeds were added to each petri dish. All petri
dishes were put in constant temperature of 15 0C incubator.
The results showed that the SISIR water extract had a greater
inhibitory effects on germination and primary growth of wheat
than those of DESSO water extract. The water extracts of the
leaves of both weeds had the greatest inhibitory effects on
germination and primary growth of wheat, compared to those of
stems and roots. Increasing the concentration of water extract of
leaves, stems and roots of both weeds up to 8 % caused the greatest
inhibitory effects to wheat and reduced the germination rate and
primary growth of it linearly.
Abstract: 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) catalyzes the conversion of HMG-CoA to mevalonate using NADPH and the enzyme is involved in rate-controlling step of mevalonate. Inhibition of HMGR is considered as effective way to lower cholesterol levels so it is drug target to treat hypercholesterolemia, major risk factor of cardiovascular disease. To discover novel HMGR inhibitor, we performed structure-based pharmacophore modeling combined with molecular dynamics (MD) simulation. Four HMGR inhibitors were used for MD simulation and representative structure of each simulation were selected by clustering analysis. Four structure-based pharmacophore models were generated using the representative structure. The generated models were validated used in virtual screening to find novel scaffolds for inhibiting HMGR. The screened compounds were filtered by applying drug-like properties and used in molecular docking. Finally, four hit compounds were obtained and these complexes were refined using energy minimization. These compounds might be potential leads to design novel HMGR inhibitor.
Abstract: White rust, caused by Albugo candida, is the most
destructive foliar diseases of persian cress, Lepidium sativum in Iran.
Application of fungicide is the most common method for the disease
control. However, regarding the problems created by synthetic
pesticides application, environmentally safe methods are needed to
replace chemical pesticides. In this study, the antifungal activity of
plant natural extracts was investigated for their ability to inhibit
zoospore release from sporangia of A. candida. The crude extract of
46 plants was obtained using methanol. The inhibitory effect of the
extracts was examined by mixing the plant extracts with a
zoosporangial suspension of A. candida (1×106 spore/ml) at three
concentrations, 250, 100 and 50 ppm. The experiments were
conducted in a completely randomized design, with three replicates.
The results of the experiment showed that three out of 46 plants
species, including, Rhus coriaria, Anagallis arvensis and Mespilus
germanica were completely inhibit zoospore release from
zoosporangia of Albugo candida at concentration of 50 ppm.
Abstract: Lactic acid alone and its combined application with
nisin were evaluated for reducing population of naturally occurring
microorganisms on chilled shrimp. Fresh shrimps were dipped in 0,
1.0% and 2.0% (v/v) lactic acid alone and their combined application
with 0.04 (g/L/kg) nisin solution for 10 min. Total plate counts of
aerobic bacteria (TPCs), Psychrotrophic counts, population of
Pseudomonas spp., H2S producing bacteria and Lactic acid bacteria
(LAB) on shrimps were determined during storage at 4 °C. The
results indicated that total plate counts were 2.91 and 2.63 log CFU/g
higher on untreated shrimps after 7 and 14 days of storage,
respectively, than on shrimps treated with 2.0% lactic acid combined
with 0.04 (g/L/kg) nisin. Both concentrations of lactic acid indicated
significant reduction on Pseudomonas counts during storage, while
2.0% lactic acid combined with nisin indicated the highest reduction.
In addition, H2S producing bacteria were more sensitive to high
concentration of lactic acid combined with nisin during storage.
Abstract: EcoDam is an adenine-N6 DNA methyltransferase
that methylates the GATC sites in the Escherichia coli genome.
DNA-adenine methylation is not present in higher eukaryotes
including humans. These observations raise the possibility that dam
inhibitors may be used as anti-microbial agents. Polyphosphate
(Poly(P)) is an important metabolite and signaling molecule in
prokaryotes and eukaryotes. Here, by using gel retardation
experiments to investigate the competition of DNA binding by
EcoDam in the presence of polyphosphate, we found that Poly (P)
strongly interferes with DNA binding by EcoDam, while same
concentration of monophosphate does not. In addition, we
demonstrated that Poly (P) binding inhibits the activity of EcoDam
and our results suggest that Poly (P) led to strong inhibition of the
EcoDam catalytic activity, while monophosphate had only moderate
effect.
Abstract: Our results showed that treatment with both
cyclooxygenase (COX1 or COX2) inhibitors impair reproduction
parameters of the medaka. Resveratrol (COX1 inhibitor) caused an
decrease in the number of spawning females at the first week of
feeding fish with experimental diets. In the group treated with NS-
398 (COX2 inhibitor) we found the lowest sperm velocity parameters
and decreased linearity of movement. The ovaries of the medaka fed
feed supplemented with Resveratrol or NS-398 were confirmed to
have a lower share of matured oocytes however during the
experiment (four weeks) the number of eggs spawned by females was
similar. Both inhibitors in fish diet (20 mg/kg body weight/day)
caused a decrease in the embryo survival. Our results revealed that
for the medaka female reproduction, activity of both COX enzymes
might be necessary whereas males reproduction competence, as
expressed by sperm motility parameters, might be related to COX2
activity.
Abstract: There was a high rate of corrosion in Pyrolysis
Gasoline Hydrogenation (PGH) unit of Arak Petrochemical Company
(ARPC), and it caused some operational problem in this plant. A
commercial chemical had been used as anti-corrosion in the
depentanizer column overhead in order to control the corrosion rate.
Injection of commercial corrosion inhibitor caused some
operational problems such as fouling in some heat exchangers. It was
proposed to replace this commercial material with another more
effective trouble free, and well-known additive by R&D and
operation specialists.
At first, the system was simulated by commercial simulation
software in electrolytic system to specify low pH points inside the
plant. After a very comprehensive study of the situation and technical
investigations ,ammonia / monoethanol amine solution was proposed
as neutralizer or corrosion inhibitor to be injected in a suitable point
of the plant. For this purpose, the depentanizer column and its
accessories system was simulated again in case of this solution
injection.
According to the simulation results, injection of new anticorrosion
substance has no any side effect on C5 cut product and
operating conditions of the column. The corrosion rate will be
cotrolled, if the pH remains at the range of 6.5 to 8 . Aactual plant
test run was also carried out by injection of ammonia / monoethanol
amine solution at the rate of 0.6 Kg/hr and the results of iron content
of water samples and corrosion test coupons confirmed the
simulation results.
Now, ammonia / monoethanol amine solution is injected to a
suitable pint inside the plant and corrosion rate has decreased
significantly.
Abstract: In production of medicinal plants, seed germination is
very important problem. The treated seeds (control, hydro priming
and ZnSO4) of Cumin (Cuminum cyminum L.) were evaluated at
germination and seedling growth for tolerance to salt (NaCl and
Na2SO4) conditions at the same water potentials of 0.0, -0.3, -0.6, -
0.9 and -1.2MPa. Electrical conductivity (EC) values of the NaCl
solutions were 0.0, 6.5, 12.7, 18.4 and 23.5 dSm-1, respectively. The
objective of the study was to determine factors responsible for
germination and early seedling growth due to salt toxicity or osmotic
effect and to optimize the best priming treatment for these stress
conditions. Results revealed that germination delayed in both
solutions, having variable germination with different priming
treatments. Germination, shoot and weight, root and shoot length
were higher but mean germination time and abnormal germination
percentage were lower in NaCl than Na2SO4 at the same water
potential. The root / shoot weight and R/S length increased with
increase in osmotic potential in both NaCl and Na2SO4 solutions.
NaCl had less inhibitor effect on seedling growth than the
germination. It was concluded that inhibition of germination at the
same water potential of NaCl and Na2SO4 resulted from salt toxicity
rather than osmotic effect. Hydro priming increased germination and
seedling growth under salt stress. This protocol has practical
importance and could be recommended to farmers to achieve higher
germination and uniform emergence under field conditions.
Abstract: Gas hydrates form when a number of factors co-exist:
free water, hydrocarbon gas, cold temperatures and high pressures are typical of the near mud-line conditions in a deepwater drilling
operation. Subsequently, when drilling with water based muds, particularly on exploration wells, the risk of hydrate formation
associated with a gas influx is high. The consequences of gas hydrate
formation while drilling are severe, and as such, every effort should be made to ensure the risk of hydrate formation is either eliminated
or significantly reduced. Thermodynamic inhibitors are used to reduce the free water content of a drilling mud, and thus suppress the
hydrate formation temperature. Very little experimental work has
been performed by oil and gas research companies on the evaluation
of gas hydrate formation in a water-based drilling mud. The main
objective of this paper is to investigate the experimental gas hydrate
formation for a mixture of methane, carbon dioxide & nitrogen in a
water-based drilling mud with or without presence of different
concentrations of thermodynamic inhibitors including pure salt and a
combination of salt with methanol or ethylene glycol at different
concentrations in a static loop apparatus. The experiments were
performed using a static loop apparatus consisting of a 2.4307 cm
inside diameter and 800 cm long pipe. All experiments were conducted at 2200 psia. The temperature in the loop was decreased at
a rate of 3.33 °F/h from initial temperature of 80 °F.
Abstract: A three-year field experiment (2010-2012) was
conducted to determine the abundance of epiphytic and endophytic
filamentous fungi colonizing the grain of winter wheat cv. Bogatka.
Wheat spikes were protected with tebuconazole or chitosan at the
watery ripe stage. Untreated plants served as control. Tebuconazole
exerted an inhibitory effect primarily on F. culmorum and F.
graminearum, and its effectiveness was determined by the pressure
from pathogens that infected wheat spikes during the growing season.
Chitosan did not suppress the growth of Fusarium species and
Alternaria alternata.
Abstract: Characterization and evaluation of the activity of Vespa basalis DPP-IV, which expressed in Spodoptera frugiperda 21 cells. The expression of rDPP-IV was confirmed by SDS–PAGE, Western blot analyses, LC-MS/MS and measurement of its peptidase specificity. One-step purification by Ni-NTA affinity chromatography and the total amount of rDPP-IV recovered was approximately 6.4mg per liter from infected culture medium; an equivalent amount would be produced by 1x109 infected Sf21 insect cells. Through the affinity purification led to highly stable rDPP-IV enzyme was recovered and with significant peptidase activity. The rDPP-IV exhibited classical Michaelis–Menten kinetics, with kcat/Km in the range of 10-500 mM-1×S-1 for the five synthetic substrates and optimum substrate is Ala-Pro-pNA. As expected in inhibition assay, the enzymatic activity of rDPP-IV was significantly reduced by 80 or 60% in the presence of sitagliptin (a DPP-IV inhibitor) or PMSF (a serine protease inhibitor), but was not apparently affected by iodoacetamide (a cysteine protease inhibitor).
Abstract: Every 2-3 years the influenza B virus serves
epidemics. Neuraminidase (NA) is an important target for influenza
drug design. Although, oseltamivir, an oral neuraminidase drug, has
been shown good inhibitory efficiency against wild-type of influenza
B virus, the lower susceptibility to the R152K mutation has been
reported. Better understanding of oseltamivir efficiency and
resistance toward the influenza B NA wild-type and R152K mutant,
respectively, could be useful for rational drug design. Here, two
complex systems of wild-type and R152K NAs with oseltamivir
bound were studied using molecular dynamics (MD) simulations.
Based on 5-ns MD simulation, the loss of notable hydrogen bond and
decrease in per-residue decomposition energy from the mutated
residue K152 contributed to drug compared to those of R152 in wildtype
were found to be a primary source of high-level of oseltamivir
resistance due to the R152K mutation.