Phytotoxicity of Daphne Gnidium L. Occurring in Tunisia

Phytotoxicity of Daphne gnidium L. was evaluated through the effect of incorporating leaves, stems and roots biomass into soil (at 12.5, 25, 50g/Kg) and irrigation by their aqueous extracts (50g/L), on the growth of two crops (Lactuca sativa L. and Raphanus sativus L.) and two weeds (Peaganum harmala L. and Scolymus maculatus L.). Results revealed a perceptible phytotoxic effect which increased with dose and concentration. At the highest dose, roots and leaves residues was the most toxic and caused total inhibition respectively, for lettuce and thistle seedling growth. Irrigation with aqueous extracts of D. gnidium different organs decreased also seedlings length of all test species. Stems extract was more inhibitor on thistle than peganum seedling growth; it induced a significant reduction of 80% and 67%, for, respectively, roots and shoots. Results of the present study suggest that different organs of D. gnidium could be exploited in the management of agro-ecosystems.

Authors:

• Ladhari A.
• Omezzine F.
• Rinez A.
• Haouala R.

Keywords:

• Biomass
• Daphne gnidium L.
• phytoxicity
• seedlinggrowth

References:

[1] S.S. Narwal, "Allelopathy in ecological sustainable organic agriculture,"
Allelopathy. J., Vol. 25, no. 1, pp. 51-72, 2010.
[2] C.H. Chou, "Role of allelopathy in sustainable agriculture: Use of
allelochemicals as naturally occurring bio-agrochemicals," Allelopathy.
J., Vol. 25, no. 1, pp. 3-16, 2010.
[3] H. Molisch, ÔÇÿ-Der Einfluss einer Pflanze auf die andere-Allelopathie,"
Fischer Jena Germany, 1937.
[4] M.A.B. Mallik and R.D. Williams, "Allelopathic principles for
sustainable agriculture," Allelopathy. J., Vol. 24, no. 1, pp. 1-34, 2009.
[5] W. Chaouki , DY. Leger , B. Liagre , Y. Cherrah , JL. Beneytout and M.
Hmamouchi , "Roots of Daphne gnidium L. inhibit cell proliferation
and induce apoptosis in the human breast cancer cell line MCF-7,"
Pharmazie, Vol. 64, no.8, pp.542, Aug. 2009.
[6] R.P Borris, P.G. Blasko and G.A. Cordell, "Ethnopharmacologic and
phytochemical studies of the Thymelaeaceae," Ethnopharmacol. J., Vol.
24, pp.41-91, 1998.
[7] J. Bellakhdar, R. Claisse, J. Fleurentin and C. Younos, "Repertory of
standard herbal drugs in the Moroccan pharmacopoeia,"
Ethnopharmacol. J., Vol. 35, pp. 123-143, 1991.
[8] Le. Floc-h, "Contribution a une etude ethnobotanique de la flore
Tunisienne, Ministere de l-enseignement superieur et la recherche
scientifique deuxieme partie, Imp. Off. Rep. Tunisia, 1983.
[9] L. Maistrello, M. L├│pez, F. Soria and R. Ocete, "Growth inhibitory
activity of Daphne gnidium L. (Thymelaeaceae) extracts on the elm leaf
beetle (Col., Chrysomelidae)," Appl. Entomol. J., Vol. 129, pp. 418-
424, 2005.
[10] H. Harizi, Fadwa Chaabane , Kamel Ghedira and Leila Chekir-Ghedira,
"Inhibition of proinflammatory macrophage responses and lymphocyte
proliferation in vitro by ethyl acetate leaf extract from Daphne
gnidium", Cell. Immunol., Vol. 267, pp. 94-101, 2011.
[11] F., Cottiglia, G. Loy, D. Garau, C. Floris, M. Casu, R. Pompei and L.
Bonsignore, "Antimicrobial evaluation of coumarins and flavonoids
from the stems of Daphne gnidium L.," Phytomedicine, Vol. 8, no. 4,
pp. 302-305, 2001.
[12] L. Iauk, G. Aleo, F. Caccamo, A. Rapisarda, S. Ragusa and A.M.
Speciale, "Antibacterial and Antimycotic Activities of Daphne gnidium
L. Leaf Extracts," Phytother. Res.,Vol.10, pp.166-168, 1996.
[13] L. Iauk, G. Aleo, F. Caccamo, A. Rapisarda, S. Ragusa and A.M.
Speciale, "Comparative Evaluation of Antibacterial and Antimycotic
Activities of Daphne gnidium Leaf and Bark Extracts," Farmaci.
Terapia., Vol. 14, pp. 37-43, 1997.
[14] M. Deiana, A. Rosaa, V. Casu, F. Cottiglia, L. Bonsignore and M.A.
Dessì, "Chemical Composition and Antioxidant Activity of Extracts
from Daphne gnidium L,". J.A.O.C.S., Vol. 80, no. 1, 2003.
[15] E. Cabrera, A. García-Granados, "Fitoquimica de Thymeleaces (III):
componentes cumarinicos y flavonicos en hojas de Daphne gnidium L.
," Anales de Quimica.,Vol. 77, pp. 31-34, 1981.
[16] J. Ragot, P. Tubery, M. Carreras-Jansou, A. Lattes and P. Symonds,
"Isolament de la 5 primeverosyl genkwanine des racines de Daphne
gnidium L," Fitoterapia., Vol. 59, pp. 336-337,1988.
[17] J. Arshad, S. Shazia, S. Sobiya, and R. Tariq, "Effects of rice extracts
and residue incorporation on Parthenium hysterophoru. Management,"
Allelopathy. J., Vol. 22, pp. 353-362, 2008.
[18] AN., Seal, JE. Pratley, TJ. Haig, M. An, H. Wu, "Plants with phytotoxic
potential: Wollemi pine (Wollemia nobilis)," Agric. Ecosyst.Environ.,
Vol. 135, pp. 52-57, 2010.
[19] Z.A Cheema, A. Khaliq and S. Saeed, "Weed control in maize (Zea
mays L.) through sorghum allelopathy," Sustain. Agric. Environ., Vol.
23, pp. 73-86, 2004.
[20] T.D. Khanh, I.M. Chung, , S. Tawata, T.D. Xuan, "Weed suppression by
Passiflora edulis and its potential allelochemicals," Weed Res., Vol. 46,
pp. 296-303, 2006.
[21] A.A. El-Khatib, A.K. Hegazy and H.K. Galal, "Allelopathy in the
rhizosphere and amended soil of Chenopodium murale L.," Weed Biol.
Manage., Vol. 4, pp. 35-42 , 2004
[22] K. Kobayashi, "Factors affecting phytotoxic activity of allelochemicals
in soil," Weed Biol. Manage., Vol. 4, pp. 1-7, 2004.
[23] A.M. Tawaha, M.A. Turk, "Allelopathic effects of Black Mustard
(Brassica nigra) on germination and growth of wild barley (Hordeum
spontaneum)," J. Agron. Crop Sci., Vol. 189, pp. 298-303, 2003
[24] T.D. Xuan, T. Shinkichi, T.D. Khanh and I.M. Chung, "Biological
control of weeds and plant pathogens in paddy rice by exploiting plant
allelopathy: an overview," Crop Protect., Vol. 24, pp. 197-206, 2005.
[25] M.M. Williams, D.A. Mortensen and J.W. Doran, "Assessment of weed
and crop fitness in cover crop residues for integrated weed
management," Weed Science., Vol. 46, pp. 595-605, 1998.
[26] M.A. Turk and A.M Tawaha, "Inhibitory effects of aqueous extracts of
black mustard on germination and growth of lentil," Pakistan Journal of
Agronomy., Vol. 1, pp. 28-30, 2002.
[27] D.R. Batish, M. Kaur, H.P. Singh and R.K. Kohli, "Phytotoxicity of a
medicinal plant, Anisomeles indica, against Phalaris minor and its
potential use as natural herbicide in wheat fields," Crop Prot., Vol. 26,
pp. 948-952, 2006.
[28] Z.S. Siddiqui, "Allelopathic effects of black pepper leachings on Vigna
mungo (L.) Hepper," Acta Physiol. Plant.,Vol. 29, pp. 303-308, 2007.
[29] C.M. Han, K.W. Pan, N. Wu, J.C. Wang and W. Li, "Allelopathic
effect of ginger on seed germination and seedling growth of soybean and
chive," Sci. Hortic., Vol. 116, pp. 330-336, 2008.
[30] Ma. Lin, Wu. Hongli, Bai. Ru, Zhou. Li, Yuan. Xiaohong and Hou.
Dabin, "Phytotoxic effects of Stellera chamaejasme L. root extract,"
A.J.A.R., Vol. 6, no.5, pp. 1170-1176, 2011.
[31] MR. Abenavoli, A. Sorgona, M. Sidari, M. Badiani and A.
Fuggi, "Coumarin inhibits the growth of carrot (Daucus carota L. cv.
Saint Valery) cells in suspension culture," J. Plant. Physiol., Vol. 160,
pp. 227-237, 2003.
[32] K. Kobayashi , D. Itaya, P. Mahatamnuchoke and T. Pornprom,
"Allelopathic potential of itchgrass (Rottboellia exaltata L.f.) powder
incorporated into soil,"Weed Biol Manag., Vol. 8, pp. 64-68, 2008.
[33] D.N. Choesin and R.E.J. Boerner, "Allyl isothiocyanate release and the
allelopathic potential of Brassica napus (Brassicaceae)," AM. J. BOT.,
Vol. 78, pp. 1083-1090, 1991.