The Effect of Selective Cyclooxygenase (COX) Inhibitors on Japanese Medaka (Oryzias latipes) Reproduction Parameters
Our results showed that treatment with both
cyclooxygenase (COX1 or COX2) inhibitors impair reproduction
parameters of the medaka. Resveratrol (COX1 inhibitor) caused an
decrease in the number of spawning females at the first week of
feeding fish with experimental diets. In the group treated with NS-
398 (COX2 inhibitor) we found the lowest sperm velocity parameters
and decreased linearity of movement. The ovaries of the medaka fed
feed supplemented with Resveratrol or NS-398 were confirmed to
have a lower share of matured oocytes however during the
experiment (four weeks) the number of eggs spawned by females was
similar. Both inhibitors in fish diet (20 mg/kg body weight/day)
caused a decrease in the embryo survival. Our results revealed that
for the medaka female reproduction, activity of both COX enzymes
might be necessary whereas males reproduction competence, as
expressed by sperm motility parameters, might be related to COX2
activity.
[1] E. Candelario-Jalil, A. C. P. De Oliveira, S. Gräf, H.S. Bhatia, M. Hüll,
E. Mu├▒oz and B.,L. Fiebich, "Resveratrol potently reduces prostaglandin
E2 production and free radical formation in lipopolysaccharide-activated
primary ramicroglia". J. Neuroinflammation 4: 25, 2007.
[2] M. R. Castro, D. Lutz and J.L. Edelman, "Effect of COX inhibitors on
VEGF-induced retinal vascular leakage and experimental corneal and
choroidal neovascularization". Exp Eye Res 79(2): 275-285, 2004.
[3] Y. I. Cha, S. H. Kim, L. Solnica-Krezel and R. N. DuBois,
"Cyclooxygenase-1 signaling is required for vascular tube formation
during development". Dev. Biol. 282: 274-283, 2005.
[4] J. L. Flippin, D. Hugget and C. M. Foran, "Changesin the timing of
reproduction following chronic exposure to ibuprofen in medaka,
Oryzias latipes". Aquat. Toxicol. 81: 73-78, 2007.
[5] Ch. Fujimori, K. Ogiwara, A. Hagiwara, S. Rajapakse, A. Kimura and T.
Takahashi,"Expression of cyclooxygenase-2 and prostaglandin receptor
EP4b mRNA in the ovary of the medaka fish, Oryzias latipes: Possible
involvement in ovulation". Mol. Cell. Endocrinol. 332: 67-77, 2011.
[6] M. J. G. Gage, C. P. Macfarlane, S. Yeates, R. G. Ward, J. B. Searle and
G. A. Parker, "Spermatozoal traits and sperm competition in Atlantic
salmon: relative sperm velocity is the primary determinant of
fertilization success". Curr. Biol. 14: 44-47, 2004.
[7] T. Grosser, S. Yusuff, E. Cheskis, M. A. Pack and G. A. Fitzgerald,
"Development expression of functional cyclooxygenases in zebrafish".
Proc. Natl. Acad. Sci., 99: 8418-8423, 2002.
[8] S. Han, K. Choi, J. Kim, K. Ji , S. Kim, B. Ahn, J. Yun, K. Choi, J. S.
Khim, X. Zhang and J. P. Giesy, "Endocrine disruption and
consequences of chronic exposure to ibuprofen in Japanese medaka
(Oryzias latipes) and freshwater cladocerans Daphnia magna and Moina
macrocopa". Aquat. Toxicol. 98: 256-264, 2010.
[9] J. C. Havird, M. M. Miyamoto, K. P. Choe and D.H. Evans, "Gene
duplications and losses within the cyclooxygenase family of teleosts and
other chordates". Mol. Biol. Evol. 25: 2349-2359, 2008.
[10] H. N. Hong, H. N. Kim, K. S. Park, S. K. Lee and M. B. Gu, "Analysis
of the effects diclofenac has on Japanese medaka (Oryzias latipes) using
real-time PCR". Aquat. Toxicol. 67: 2115-2121, 2007.
[11] Y. Ishikawa "Medaka fish as a model for vertebrate developmental.
Genetics". Bioessays 22, 487-495, 2000.
[12] T. Iwamatsu, T. Ohta, E. Oshima and N. Sakai, "Oogenesis in the
medaka Oryzias latipes-stages of oocyte development". Zool. Sci. 5:
353-373, 1988.
[13] M. S. Izoquiredo, H. Fernandez-Palacios and A. G. J. Tacon, "Effect of
broodstock nutrition on reproductive performance of fish". Aquaculture
197: 25 - 42, 2001.
[14] M. Jang and J. M. Pezzuto, "Resveratrol blocks eicosanoid production
and chemicallyinduced cellular transformation: implications for cancer
chemoprevention". Pharmaceut. Biol. 36: 28-34, 1998.
[15] J. K. Kennedy, N. Korn and R. J. Thurston, "Prostaglandin levels in
seminal plasma and sperm extracts of the domestic turkey, and the
effects of cyclooxygenase inhibitors on sperm mobility". Reprod Bio.
Endocrinol. 9: 74-81, 2003.
[16] M. Kinoshita, K. Murata, K. Naruse and M. Tanaka, "Medaka
management". In: Medaka. Biology, management, and experimental
protocols M. Kinoshita, K. Murata, K. Naruse, M. Tanaka, Eds. USA:
Wiley-Blackwell, 2009, pp. 31-65.
[17] G. M. Rocha, L. F. Michea, E. M. Peters, M. Kirby, Y. Xu, D. R
Ferguson and M. B. Burg, "Direct toxicity of nonsteroidal antiinflammatory
drugs for renal medullary cells". Proc Natl Acad Sci 98:
5317-5322, 2001.
[18] J. R. Sargent, D. R. Tocher and J. G. Bell, "The Lipids". In: Fish
Nutrition J. E. Halver, R. Hardy, Eds. CA: San Diego, Academic Press,
2002, pp. 182-246.
[19] D. R. Valenzano, E. Terzibasi, T. Genade, A. Cattaneo, L. Domenici and
A. Cellerino, "Resveratrol prolongs lifespan and retards the onset of agerelated
markers in a short-lived vertebrate". Curr Biol. 7:16(3): 296-300,
2006.
[20] H. Yang and T. R. Tiersch, "Sperm motility initiation and duration in a
euryhaline fish, medaka (Oryzias latipes)". Theriogenology, 72(3): 386-
392, 2009.
[1] E. Candelario-Jalil, A. C. P. De Oliveira, S. Gräf, H.S. Bhatia, M. Hüll,
E. Mu├▒oz and B.,L. Fiebich, "Resveratrol potently reduces prostaglandin
E2 production and free radical formation in lipopolysaccharide-activated
primary ramicroglia". J. Neuroinflammation 4: 25, 2007.
[2] M. R. Castro, D. Lutz and J.L. Edelman, "Effect of COX inhibitors on
VEGF-induced retinal vascular leakage and experimental corneal and
choroidal neovascularization". Exp Eye Res 79(2): 275-285, 2004.
[3] Y. I. Cha, S. H. Kim, L. Solnica-Krezel and R. N. DuBois,
"Cyclooxygenase-1 signaling is required for vascular tube formation
during development". Dev. Biol. 282: 274-283, 2005.
[4] J. L. Flippin, D. Hugget and C. M. Foran, "Changesin the timing of
reproduction following chronic exposure to ibuprofen in medaka,
Oryzias latipes". Aquat. Toxicol. 81: 73-78, 2007.
[5] Ch. Fujimori, K. Ogiwara, A. Hagiwara, S. Rajapakse, A. Kimura and T.
Takahashi,"Expression of cyclooxygenase-2 and prostaglandin receptor
EP4b mRNA in the ovary of the medaka fish, Oryzias latipes: Possible
involvement in ovulation". Mol. Cell. Endocrinol. 332: 67-77, 2011.
[6] M. J. G. Gage, C. P. Macfarlane, S. Yeates, R. G. Ward, J. B. Searle and
G. A. Parker, "Spermatozoal traits and sperm competition in Atlantic
salmon: relative sperm velocity is the primary determinant of
fertilization success". Curr. Biol. 14: 44-47, 2004.
[7] T. Grosser, S. Yusuff, E. Cheskis, M. A. Pack and G. A. Fitzgerald,
"Development expression of functional cyclooxygenases in zebrafish".
Proc. Natl. Acad. Sci., 99: 8418-8423, 2002.
[8] S. Han, K. Choi, J. Kim, K. Ji , S. Kim, B. Ahn, J. Yun, K. Choi, J. S.
Khim, X. Zhang and J. P. Giesy, "Endocrine disruption and
consequences of chronic exposure to ibuprofen in Japanese medaka
(Oryzias latipes) and freshwater cladocerans Daphnia magna and Moina
macrocopa". Aquat. Toxicol. 98: 256-264, 2010.
[9] J. C. Havird, M. M. Miyamoto, K. P. Choe and D.H. Evans, "Gene
duplications and losses within the cyclooxygenase family of teleosts and
other chordates". Mol. Biol. Evol. 25: 2349-2359, 2008.
[10] H. N. Hong, H. N. Kim, K. S. Park, S. K. Lee and M. B. Gu, "Analysis
of the effects diclofenac has on Japanese medaka (Oryzias latipes) using
real-time PCR". Aquat. Toxicol. 67: 2115-2121, 2007.
[11] Y. Ishikawa "Medaka fish as a model for vertebrate developmental.
Genetics". Bioessays 22, 487-495, 2000.
[12] T. Iwamatsu, T. Ohta, E. Oshima and N. Sakai, "Oogenesis in the
medaka Oryzias latipes-stages of oocyte development". Zool. Sci. 5:
353-373, 1988.
[13] M. S. Izoquiredo, H. Fernandez-Palacios and A. G. J. Tacon, "Effect of
broodstock nutrition on reproductive performance of fish". Aquaculture
197: 25 - 42, 2001.
[14] M. Jang and J. M. Pezzuto, "Resveratrol blocks eicosanoid production
and chemicallyinduced cellular transformation: implications for cancer
chemoprevention". Pharmaceut. Biol. 36: 28-34, 1998.
[15] J. K. Kennedy, N. Korn and R. J. Thurston, "Prostaglandin levels in
seminal plasma and sperm extracts of the domestic turkey, and the
effects of cyclooxygenase inhibitors on sperm mobility". Reprod Bio.
Endocrinol. 9: 74-81, 2003.
[16] M. Kinoshita, K. Murata, K. Naruse and M. Tanaka, "Medaka
management". In: Medaka. Biology, management, and experimental
protocols M. Kinoshita, K. Murata, K. Naruse, M. Tanaka, Eds. USA:
Wiley-Blackwell, 2009, pp. 31-65.
[17] G. M. Rocha, L. F. Michea, E. M. Peters, M. Kirby, Y. Xu, D. R
Ferguson and M. B. Burg, "Direct toxicity of nonsteroidal antiinflammatory
drugs for renal medullary cells". Proc Natl Acad Sci 98:
5317-5322, 2001.
[18] J. R. Sargent, D. R. Tocher and J. G. Bell, "The Lipids". In: Fish
Nutrition J. E. Halver, R. Hardy, Eds. CA: San Diego, Academic Press,
2002, pp. 182-246.
[19] D. R. Valenzano, E. Terzibasi, T. Genade, A. Cattaneo, L. Domenici and
A. Cellerino, "Resveratrol prolongs lifespan and retards the onset of agerelated
markers in a short-lived vertebrate". Curr Biol. 7:16(3): 296-300,
2006.
[20] H. Yang and T. R. Tiersch, "Sperm motility initiation and duration in a
euryhaline fish, medaka (Oryzias latipes)". Theriogenology, 72(3): 386-
392, 2009.
@article{"International Journal of Biological, Life and Agricultural Sciences:55274", author = "Agata Kowalska and Radosław K. Kowalski and Zdzisław Zakęś", title = "The Effect of Selective Cyclooxygenase (COX) Inhibitors on Japanese Medaka (Oryzias latipes) Reproduction Parameters", abstract = "Our results showed that treatment with both
cyclooxygenase (COX1 or COX2) inhibitors impair reproduction
parameters of the medaka. Resveratrol (COX1 inhibitor) caused an
decrease in the number of spawning females at the first week of
feeding fish with experimental diets. In the group treated with NS-
398 (COX2 inhibitor) we found the lowest sperm velocity parameters
and decreased linearity of movement. The ovaries of the medaka fed
feed supplemented with Resveratrol or NS-398 were confirmed to
have a lower share of matured oocytes however during the
experiment (four weeks) the number of eggs spawned by females was
similar. Both inhibitors in fish diet (20 mg/kg body weight/day)
caused a decrease in the embryo survival. Our results revealed that
for the medaka female reproduction, activity of both COX enzymes
might be necessary whereas males reproduction competence, as
expressed by sperm motility parameters, might be related to COX2
activity.", keywords = "COX innibitors, medaka, reproduction parameters", volume = "5", number = "5", pages = "272-5", }
