Abstract: Lanthanide-doped upconversion nanoparticles which can convert near-infrared lights to visible lights have attracted growing interest because of their great potentials in fluorescence imaging. Upconversion fluorescence imaging technique with excitation in the near-infrared (NIR) region has been used for imaging of biological cells and tissues. However, improving the detection sensitivity and decreasing the absorption and scattering in biological tissues are as yet unresolved problems. In this present study, a novel NIR-reflected multispectral imaging system was developed for upconversion fluorescent imaging in small animals. Based on this system, we have obtained the high contrast images without the autofluorescence when biocompatible UCPs were injected near the body surface or deeply into the tissue. Furthermore, we have extracted respective spectra of the upconversion fluorescence and relatively quantify the fluorescence intensity with the multispectral analysis. To our knowledge, this is the first time to analyze and quantify the upconversion fluorescence in the small animal imaging.
Abstract: In the paper the study of synthetic transmit aperture
method applying the Golay coded transmission for medical
ultrasound imaging is presented. Longer coded excitation allows to
increase the total energy of the transmitted signal without increasing
the peak pressure. Moreover signal-to-noise ratio and penetration
depth are improved while maintaining high ultrasound image
resolution. In the work the 128-element linear transducer array with
0.3 mm inter-element spacing excited by one cycle and the 8 and 16-
bit Golay coded sequences at nominal frequency 4 MHz was used. To
generate a spherical wave covering the full image region a single
element transmission aperture was used and all the elements received
the echo signals. The comparison of 2D ultrasound images of the
tissue mimicking phantom and in vitro measurements of the beef liver
is presented to illustrate the benefits of the coded transmission. The
results were obtained using the synthetic aperture algorithm with
transmit and receive signals correction based on a single element
directivity function.
Abstract: The last years have seen an increasing use of image analysis techniques in the field of biomedical imaging, in particular in microscopic imaging. The basic step for most of the image analysis techniques relies on a background image free of objects of interest, whether they are cells or histological samples, to perform further analysis, such as segmentation or mosaicing. Commonly, this image consists of an empty field acquired in advance. However, many times achieving an empty field could not be feasible. Or else, this could be different from the background region of the sample really being studied, because of the interaction with the organic matter. At last, it could be expensive, for instance in case of live cell analyses. We propose a non parametric and general purpose approach where the background is built automatically stemming from a sequence of images containing even objects of interest. The amount of area, in each image, free of objects just affects the overall speed to obtain the background. Experiments with different kinds of microscopic images prove the effectiveness of our approach.