Abstract: Lactobionic acid is a disaccharide formed from gluconic acid and galactose, and produced by oxidation of lactose. Productivity of lactobionic acid by microbial synthesis can be affected by various factors, and one of them is a presence of potassium, magnesium and manganese ions. In order to extend lactobionic acid production efficiency, it is necessary to increase the yield of lactobionic acid by optimising the fermentation conditions and available substrates for Pseudomonas taetrolens growth. The object of the research was to determinate the application of K2HPO4, MnSO4, MgSO4 × 7H2O salts in different concentration for effective lactose oxidation to lactobionic acid by Pseudomonas taetrolens. Pseudomonas taetrolens NCIB 9396 (NCTC, England) and Pseudomonas taetrolens DSM 21104 (DSMZ, Germany) were used for the study. The acid whey was used as the study object. The content of lactose in whey samples was determined using MilcoScanTM Mars (Foss, Denmark) and high performance liquid chromatography (Shimadzu LC 20 Prominence, Japan). The content of lactobionic acid in whey samples was determined using the high performance liquid chromatography. The impact of studied salts differs, Mn2+ and Mg2+ ions enhanced fermentation instead of K+ ions. Results approved that Mn2+ and Mg2+ ions are necessary for Pseudomonas taetrolens growth. The study results will help to improve the effectiveness of lactobionic acid production with Pseudomonas taetrolens NCIB 9396 and DSM 21104.
Abstract: Gluconic acid is one of interesting chemical products
in industries such as detergents, leather, photographic, textile, and
especially in food and pharmaceutical industries. Fermentation is an
advantageous process to produce gluconic acid. Mathematical
modeling is important in the design and operation of fermentation
process. In fact, kinetic data must be available for modeling. The
kinetic parameters of gluconic acid production by Aspergillus niger
in batch culture was studied in this research at initial substrate
concentration of 150, 200 and 250 g/l. The kinetic models used were
logistic equation for growth, Luedeking-Piret equation for gluconic
acid formation, and Luedeking-Piret-like equation for glucose
consumption. The Kinetic parameters in the model were obtained by
minimizing non linear least squares curve fitting.