Abstract: The objective of this study was to optimize the extraction conditions for phenolic compounds, total flavonoids, and antioxidant activity from Deglet-Nour variety. The extraction of active components from natural sources depends on different factors. The knowledge of the effects of different extraction parameters is useful for the optimization of the process, as well for the ability to predict the extraction yield. The effects of extraction variables, namely types of solvent (methanol, ethanol and acetone) and extraction time (1h, 6h, 12h and 24h) on phenolics extraction yield were evaluated. It has been shown that the time of extraction and types of solvent have a statistically significant influence on the extraction of phenolic compounds from Deglet-Nour variety. The optimised conditions yielded values of 80.19 ± 6.37 mg GAE/100 g FW for TPC, 2.34 ± 0.27 mg QE/100 g FW for TFC and 90.20 ± 1.29% for antioxidant activity were methanol solvent and 6 hours of time. According to the results obtained in this study, Deglet-Nour variety can be considered as a natural source of phenolic compounds with good antioxidant capacity.
Abstract: Antioxidants contribute to endogenous photoprotection
and are important for the maintenance of skin health. The study was carried out to compare the skin hydration and transepidermal
water loss (TEWL) effects of a stable cosmetic preparation
containing flavonoids, following two applications a day over a period
of tenth week. The skin trans-epidermal water loss and skin hydration
effect was measured at the beginning and up to the end of study period of ten weeks. Any effect produced was measured by Corneometer and TEWA meter (Non-invasive probe).
Two formulations were developed for this study design. Formulation one the control formulation in which no apple juice
extract( Flavonoids) was incorporated while second one was the active formulation in which the apple juice extract (3%) containing
flavonoids was incorporated into water in oil emulsion using Abil EM 90 as an emulsifier. Stable formulations (control and Active)
were applied on human cheeks (n = 12) for a study period of 10 weeks. Result of each volunteer of skin hydration and TEWL was
measured by corneometer and TEWA meter. By using ANOVA and Paired sample t test as a statistical evaluation, result of both base and
formulation were compared. Statistical significant results (p≤0.05)
were observed regarding skin hydration and TEWL when two creams, control and Formulation were compared. It showed that
desired formulation (Active) may have interesting application as an
active moisturizing cream on healthy skin.
Abstract: Ipomoea batatas (Sweetpotato) is currently ranked
sixth in the total world food production and are planted mainly for
their storage roots. The present study was undertaken to evaluate and
compare the antioxidant properties of the leaf and carotenoids extract
from the Ipomoea batatas var. Oren leaves. Total flavonoids in the
leaf extract was 144.6 ± 40.5 μg/g compared to 114.86 ± 4.35 μg/g
catechin equivalent in the carotenoids extract. Total polyphenols in
the leaf extracts (3.470 ± 0.024 GAE g/100g DW) was slightly higher
compared to carotenoids extract (2.994 ± 0.078 GAE g/100g DW).
The carotenoids extract marked a higher radical scavenging capacity
with the IC50= 491.86 μg/ml compared to leaf extract (IC50= 545.39
μg/ml). Concentration-dependent reducing activity was observed for
both extracts. Thus, the carotenoids extraction process retained most
of the antioxidant capacity from the leaves and can be made into
potential natural yellow dye with antioxidant property.
Abstract: Photoselective plastic films with thermic properties
are now available so that greenhouses clad with such plastics exhibit
a higher degree of “Greenhouse Effect” with a consequent increase in
night time temperature. In this study, we investigate the potential
benefits of a range of thermic plastic films used as greenhouse cover
materials on the vegetative and reproductive growth and development
of Iceberg lettuce (Lactuca sativa L). Transplants were grown under
thermic films and destructively harvested 4, 5, and 6 weeks after
transplanting. Thermic films can increase night temperatures up to 2
⁰C reducing the wide fluctuation in greenhouse temperature during
winter compared to the standard commercial film and consequently
increased the yield (leaf number, fresh weight, and dry weight) of
lettuce plants. Lettuce plants grown under Clear film respond to cold
stress by the accumulation of secondary products (phenolics, and
flavonoids).
Abstract: Sweet cherries (Prunus avium L.) contain various
phenolic compounds which contribute to total antioxidant activity.
Total polyphenols, tannins, flavonoids and anthocyanins, and
antioxidant capacity in a fruits of a number of selected sweet cherry
genotypes were investigated. Total polyphenols content ranged from
4.12 to 8.34 mg gallic acid equivantents/g dry fruit weight and total
tannins content ranged from 0.19 to 1.95 mg gallic acid equivalent/g
dry fruit weight. Total flavonoids were within the range 0.42-1.56 mg
of rutin equivalents/g dry fruit weight and total anthocyanins content
were between 0.35 and 0.69 mg cyanidin 3-glucoside equivalent/ g
dry fruit weight. Although sweet cherry fruits are a significant source
of different phenolic compounds, antioxidant activity of sweet
cherries is not related only with the total polyphenolics, flavonoids or
anthocyanins.
Abstract: Fruits and vegetables are the essentials of a healthy
diet, mainly because of their antioxidant properties contributing to
disease blockage especially for some certain types of cancer. Being a
favourite fruit, citrus are produced for economic and commercial
purposes worldwide. Particularly, lemon fruit (Citrus limon L.), has
an important place in export products of Turkey. Lemon has a great
importance on human nutrition with regard to being a source of
nutrients, flavonoids, vitamin C and minerals. It is used for food
flavouring and pickling and also processed for lemonade. By
processing citrus into fruit juices, consumption may increase and also
become easier. Like many fruits and vegetables lemons are cheap and
abundant during harvesting period, while they are quite expensive in
other seasons. Lemon juice and concentrate production allows
consumers to get benefits from lemon fruit in any time of the year.
Lemonade is getting in to the focus of consumers’ attention
preferring non-carbonated drinks. The demand of healthy, convenient
functional foods affects consumer trends through innovative
products. For this reason, lemonade could be enriched with different
natural herb extracts such as ginger (Zingiber officinale), linden (Tilia
cordata), and mint (Mentha piperita).
Abstract: The aim of the study was to investigate phytochemical
properties, antimicrobial activity and cytotoxicity of Aloe vera. The
phytochemical screening of the extracts of leaves of A. vera revealed
the presence of bioactive compounds such as alkaloids, tannins,
flavonoids phenolic compounds, and etc. with absence of cyanogenic
glycosides. Three different solvents such as methanol, ethanol and
Di-Methyl sulfoxide were used to screen the antimicrobial activity of
A. vera leaves against four human clinical pathogens by agar well
diffusion method. The maximum antibacterial activities were
observed in methanol extract followed by ethanol and Di-Methyl
sulfoxide. It was also found that remarkable antibacterial activities
with methanolic and ethanolic extracts of A. vera compared with the
standard antibiotic, tetracycline that was not active against E. coli
and S. boydii and supported the view that A. vera is a potent
antimicrobial agent compared with the conventional antibiotic.
Moreover, the brine shrimps (Artemia salina) toxicity test exhibited
LC50 value was 569.52 ppm. The resulting data indicated that the A.
vera plant have less toxic effects on brine shrimp. Hence, it is
signified that Aloe vera plant extract is safe to be used as an
antimicrobial agent.
Abstract: The beneficial effects of Si are mainly associated with
its high deposition in plant tissue and enhancing their strength and
rigidity. We investigated the role of Si against cadmium stress in
(Echium C) in house green condition. When the seventh leaves was
be appeared, plants were pretreated with five levels of Si: 0, 0.2, 0.5,
0.7and 1.5 mM Si (as sodium trisilicate, Na2(SiO2)3) and after that
plants were treated with two levels of Cd (30 and 90 mM). The
effects of Silicon and Cd were investigated on some physiological
and biochemical parameters such as: lipid peroxidation
(malondialdehyde (MDA) and other aldehydes, antocyanin and
flavonoid content. Our results showed that Cd significantly increased
MDA, other aldehydes, antocyanin and flavonoids content in
Echium and silicon offset the negative effect and increased tolerance
of Echium against Cd stress. From this results we concluded that Si
increase membrane integrity and antioxidative ability in this plant
against cd stress.
Abstract: Traditionally, Yemini Sidr honey has been reported to
cure liver problems, stomach ulcers, and respiratory disorders. In this
experiment, we evaluated Yemeni Sidr honey for its ability to protect
inflammations caused by acetic acid and formalin -induced writhing,
carrageenan and histamine-induced paw oedema in experimental rat
model. Hyperpyrexia, membrane stabilizing activity, and
phytochemical screening of the honey was also examined. Yemini
Sidr Honey at (100, 200 and 500 mg/kg) exhibited a concentration
dependant inhibition of acetic acid induced and formalin induced
writhing, paw oedema induced by carrageenan & histamine, and
hyperpyrexia induced by brewer's yeast, it also inhibited membrane
stabilizing activity. Phytochemical screenings of the honey reveal the
presence of flavonoids, steroid, alkaloids, saponins and tannins. This
study suggested that Yemeni Sidr honey possess very strong antiinflammatory,
analgesic and antipyretic effects and these effects
would be a result of the phytochemicals present.
Abstract: Erwinia carotovora var. carotovora is the main cause of soft rot in potatoes. Hyphaene thebaica was studied for biocontrol of E. carotovora which inhibited growth of E. carotovora on solid medium, a comparative study of classical and ultrasound-assisted extractions of Hyphaene thebaica fruit. The use of ultrasound decreased significant the total time of treatment and increase the total amount of crude extract. The crude extract was subjected to determine the in vitro, by a bioassay technique revealed that the treatment of paper disks with ultrasound extraction of Hyphaene thebaica reduced the growth of pathogen and produced inhibition zones up to 38mm in diameter. The antioxidant activity of ultrasound-ethanolic extract of Doum fruits (Hyphaene thebaica) was determined. Data obtained showed that the extract contains the secondary metabolites such as Tannins, Saponin, Flavonoids, Phenols, Steroids, Terpenoids, Glycosides and Alkaloids.