Abstract: Antioxidants contribute to endogenous photoprotection
and are important for the maintenance of skin health. The study was carried out to compare the skin hydration and transepidermal
water loss (TEWL) effects of a stable cosmetic preparation
containing flavonoids, following two applications a day over a period
of tenth week. The skin trans-epidermal water loss and skin hydration
effect was measured at the beginning and up to the end of study period of ten weeks. Any effect produced was measured by Corneometer and TEWA meter (Non-invasive probe).
Two formulations were developed for this study design. Formulation one the control formulation in which no apple juice
extract( Flavonoids) was incorporated while second one was the active formulation in which the apple juice extract (3%) containing
flavonoids was incorporated into water in oil emulsion using Abil EM 90 as an emulsifier. Stable formulations (control and Active)
were applied on human cheeks (n = 12) for a study period of 10 weeks. Result of each volunteer of skin hydration and TEWL was
measured by corneometer and TEWA meter. By using ANOVA and Paired sample t test as a statistical evaluation, result of both base and
formulation were compared. Statistical significant results (p≤0.05)
were observed regarding skin hydration and TEWL when two creams, control and Formulation were compared. It showed that
desired formulation (Active) may have interesting application as an
active moisturizing cream on healthy skin.
Abstract: This study was designed to formulate,
pharmaceutically evaluate a topical skin-care cream (w/o emulsion)
of Aloe Vera versus its vehicle (Base) as control and determine their
effects on Stratum Corneum (SC) water content and Transepidermal
water loss (TEWL). Base containing no extract and a Formulation
containing 3% concentrated extract of Aloe Vera was developed by
entrapping in the inner aqueous phase of w/o emulsion (cream).
Lemon oil was incorporated to improve the odor. Both the Base and
Formulation were stored at 8°C ±0.1°C (in refrigerator), 25°C±0.1°C,
40°C±0.1°C and 40°C± 0.1°C with 75% RH (in incubator) for a
period of 4 weeks to predict their stability. The evaluation parameters
consisted of color, smell, type of emulsion, phase separation,
electrical conductivity, centrifugation, liquefaction and pH. Both the
Base and Formulation were applied to the cheeks of 21 healthy
human volunteers for a period of 8 weeks Stratum corneum (SC)
water content and Transepidermal water loss (TEWL) were
monitored every week to measure any effect produced by these
topical creams. The expected organoleptic stability of creams was
achieved from 4 weeks in-vitro study period. Odor was disappeared
with the passage of time due to volatilization of lemon oil. Both the
Base and Formulation produced significant (p≤0.05) changes in
TEWL with respect to time. SC water content was significantly
(p≤0.05) increased by the Formulation while the Base has
insignificant (p 0.05) effects on SC water content. The newly
formulated cream of Aloe Vera, applied is suitable for improvement
and quantitative monitoring of skin hydration level (SC water
content/ moisturizing effects) and reducing TEWL in people with dry
skin.